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Full-Text Articles in Engineering
Expression Of Human Protein C In Mammary Tissue Of Transgenic Mammals , William H. Velander
Expression Of Human Protein C In Mammary Tissue Of Transgenic Mammals , William H. Velander
Department of Chemical and Biomolecular Engineering: Patents
Recombinant protein C characterized by a high percentage of active protein can be obtained in the milk of transgenic mammals that incorporate DNAs according to the present invention. Transgenic mammals of the present invention are produced by introducing into developing embryos DNA that encodes protein C, such that the DNA is stably incorporated in the DNA of germ line cells of the mature mammals and inherited in normal, mendelian fashion.
Effect Of Antibody Orientation On Immunosorbent, Anuradha Subramanian, William H. Velander
Effect Of Antibody Orientation On Immunosorbent, Anuradha Subramanian, William H. Velander
Papers in Biotechnology
The impact of antibody orientation on immunosorbent efficiency was quantitatively assessed. A pH-dependent murine monoclonal antibody (Mab) against human protein C (hPC), recombinant hPC (rhPC) and two different immobilization chemistries and matrices were used as model systems. The lysyl groups of the rhPC were covalently modified with an acetic acid ester of N-hydroxysuccinimide and this modified rhPC was used as a Fab masking agent (FMA). The FMA was used to mask the antigen bind-ing regions (Fab) of the Mab prior to and during covalent immobilization. Thereafter, the residual active sites of the support were in-activated and the FMA was removed. …
Affinity Purification Of Biologically Active Andinactive Forms Of Recombinant Human Protein C Produced In Porcine Mammary Gland, Kevin E. Van Cott, Barry Williams Department Of Chemical Engineering,Virginia Tech University, Blacksburg, Va 24061, Usa, William H. Velander, Francis C. Gwazdauskas, Tim Lee Jerome Holland Laboratory, American Red Cross, Rockville, Md 20855, Usa, Henryk Lubon, William N. Drohan
Affinity Purification Of Biologically Active Andinactive Forms Of Recombinant Human Protein C Produced In Porcine Mammary Gland, Kevin E. Van Cott, Barry Williams Department Of Chemical Engineering,Virginia Tech University, Blacksburg, Va 24061, Usa, William H. Velander, Francis C. Gwazdauskas, Tim Lee Jerome Holland Laboratory, American Red Cross, Rockville, Md 20855, Usa, Henryk Lubon, William N. Drohan
Papers in Biotechnology
Recombinant human protein C (rhPC) secreted in the milk of transgenic pigs was studied. 'Ikansgenes having different regulatory elements of the murine milk protein, whey acidic protein, were used with cDNA and genomic human protein C (hPC) DNA sequences to obtain lower and higher expressing animals. The cDNA pigs had a range of expression of about 0.1-0.5 g/l milk. Two different genomic hPC pig lines have expressed 0.3 and 1-2 g/l, respectively. The rhPC was first purified at yields greater than 60 per cent using a monoclonal antibody (mAb) to the activation site on the heavy chain of hPC. Subsequent …
Fabrication Of Double Side Fully Metallized Plated Thru-Holes, In Polymer Structures, Without Seeding Or Photoprocess, Ravi F. Saraf
Fabrication Of Double Side Fully Metallized Plated Thru-Holes, In Polymer Structures, Without Seeding Or Photoprocess, Ravi F. Saraf
Department of Chemical and Biomolecular Engineering: Patents
The present invention is directed to a process of a method for the full metallization of thru-holes in a polymer structure comprising the steps of applying a film-forming amount of a conductive polymer-metal composite paste to a metal cathode; bonding a patterned polymer structure to said paste; subjecting said polymer structure to an electrolytic plating bath for a time sufficient to fully metallize thru-hole surfaces in said patterned polymer structure and removing the structure from the cathode assembly. The fully metallized thru-hole polymer structure can then be cleaned and polished to produce a finished product.
Direct Chip Attachment (Dca) With Electrically Conductive Adhesives, Ravi F. Saraf
Direct Chip Attachment (Dca) With Electrically Conductive Adhesives, Ravi F. Saraf
Department of Chemical and Biomolecular Engineering: Patents
A simple process for card assembly by Direct Chip Attachment (DCA) uses electrically conductive adhesives. Two methods create the same intermediate wafer product with a layer of insulative thermoplastic and conductive thermoplastic bumps. After sawing or dicing the wafer to form the chips, the chips are adhered to chip carriers with conductive pads which match the conductive thermoplastic bumps, using heat and pressure. Chips may be easily removed and replaced using heat.
Sulfur Tolerant Bimetallic Zeolitic Reforming Catalysts, Gustavo F. Larsen
Sulfur Tolerant Bimetallic Zeolitic Reforming Catalysts, Gustavo F. Larsen
Department of Chemical and Biomolecular Engineering: Patents
New compositions of matter comprise a metal from the group consisting of platinum, rhodium and palladium, a metal from the first row of Group VIII of the Periodic Table and a nonacidic L-zeolite. A preferred composition is Pt--Ni/KL-zeolite. Such catalysts are prepared by coimpregnation of the zeolite with the metals. Methods of using the catalysts in reforming, aromatization or dehydrogenation are provided.
Polyelectrolyte Dna Conjugation And Genetic Transformation Of An Animal , William H. Velander
Polyelectrolyte Dna Conjugation And Genetic Transformation Of An Animal , William H. Velander
Department of Chemical and Biomolecular Engineering: Patents
Improved cellulosic beads for use as supports in bioaffinity chromatography are produced by dissolution of cellulose in a chaotropic cellulose solvent, formation of the dissolved cellulose into droplets, and immersion of the droplets into a non-solvent capable of solvent interchange with the cellulose solvent to form generally spherical porous cellulose beads of narrow particle size distribution. The beads formed are preferably made with cellulose having a degree of polymerization between 100 and 200, and the resulting beads when saturated with water without drying contain between 1% and 7% cellulose by weight and have a particle size of at least about …
Experimental And Theoretical Study Of Vibrations Of A Cantilevered Beam Using A Zno Piezoelectric Sensor, B. R. Jooste, Hendrik J. Viljoen, S. L. Rohde, N. F. J. Van Rensburg
Experimental And Theoretical Study Of Vibrations Of A Cantilevered Beam Using A Zno Piezoelectric Sensor, B. R. Jooste, Hendrik J. Viljoen, S. L. Rohde, N. F. J. Van Rensburg
Hendrik J. Viljoen Publications
Piezoelectric sensors can measure vibrations of solid structures very accurately. A model of a cantilevered beam, with a ZnO film on one side is presented. Both viscous and internal damping are considered. The output of the sensor is modeled and matched with experimental results by adjusting the damping parameters. A theoretical formulation for damage is introduced. Experimental results for a damaged beam confirm the shift in frequencies to lower values. The model is used to identify the extent of the damage.
Experimental And Theoretical Study Of Vibrations Of A Cantilevered Beam Using A Zno Piezoelectric Sensor, B. R. Jooste, Hendrik J. Viljoen, S. L. Rohde, N. F. J. Van Rensburg
Experimental And Theoretical Study Of Vibrations Of A Cantilevered Beam Using A Zno Piezoelectric Sensor, B. R. Jooste, Hendrik J. Viljoen, S. L. Rohde, N. F. J. Van Rensburg
Hendrik J. Viljoen Publications
Piezoelectric sensors can measure vibrations of solid structures very accurately. A model of a cantilevered beam, with a ZnO film on one side is presented. Both viscous and internal damping are considered. The output of the sensor is modeled and matched with experimental results by adjusting the damping parameters. A theoretical formulation for damage is introduced. Experimental results for a damaged beam confirm the shift in frequencies to lower values. The model is used to identify the extent of the damage.
Analysis Of Homogeneous Combustion In Monolithic Structures, Hendrik J. Viljoen
Analysis Of Homogeneous Combustion In Monolithic Structures, Hendrik J. Viljoen
Papers in Chemical Reactions
An analytical method is used to solve the problem of homogeneous combustion in a monolith.The solid phase acts as burner support. Heat is conducted much more effectively in the solid phase and preheating of the gas is more effective. The flame temperature exceeds the adiabatic value. Solutions are presented for different velocities and feed concentrations
Heat Transfer In Rarefied Gas At A Gas-Solid Interface, Yaşar Demirel, Satish C. Saxena
Heat Transfer In Rarefied Gas At A Gas-Solid Interface, Yaşar Demirel, Satish C. Saxena
Papers in Physical Chemistry
Modified expressions for the heat-transfer fluxes at the interface of a solid surface and a gas are given for different Knudsen-number regimes. These expressions for diatomic gases contain both a gas-reflection and a thermal accommodation coefficient. Parallel-plate, coaxial-cylindrical and concentric spherical surfaces are considered. A general expression for the thermal accommodation coefficient is based on experimental data. Sample numerical calculations of heat flux in different heat-transfer regimes are presented in terms of the reciprocal Knudsen number.