Engineering Commons^™

Effects Of Tethering Placement And Linker Variations On Antibody Stability On Surfaces, Rebecca Ellen Grawe

Theses and Dissertations

An antibody microarray consists of antibody bound to a surface. Antibody microarrays have great potential in many fields, particularly as a tool to detect antigens. Unfortunately, antibodies suffer from poor performance. A greater understanding of how antibodies interact with surfaces would improve microarray design and performance, but experimental methods fall short of being able to observe these interactions. Therefore molecular simulation has emerged as the primary method to study protein/surface interactions.The simulations here were coarse grain simulations performed using the model of Karanicolas and Brooks. Additionally, an advanced surface model was used that allows for different surface chemistries. PyMBAR analysis …

Nanoemulsions Within Liposomes For Cytosolic Drug Delivery To Multidrug-Resistant Cancer Cells, Jacob Brian Williams

Theses and Dissertations

Cancer cells that survive chemotherapy treatment often develop resistance to the administered chemotherapeutics, as well as to many other types of drugs, because the cancer cells increase their production of efflux pumps in the cell. This undesired phenomenon of resistance to cancer drugs is known as multidrug resistance. This work uses a novel drug carrier, called an eLiposome, to achieve cytosolic drug delivery to kill multidrug-resistant cancer cells. An eLiposome consists of a perfluoropentane (PFC5) emulsion droplet inside of a liposome. Folate attached to the eLiposome facilitates uptake into the cell. The PFC5 droplet is metastable at body temperature, but …

How A Systematic Approach To Uncertainty Quantification Renders Molecular Simulation A Quantitative Tool In Predicting The Critical Constants For Large N-Alkanes, Richard Alma Messerly

Theses and Dissertations

Accurate thermophysical property data are crucial for designing efficient chemical processes. For this reason, the Design Institute for Physical Properties (DIPPR 801) provides evaluated experimental data and prediction of various thermophysical properties. The critical temperature (T_c), critical density (ρ_c), critical pressure (P_c), critical compressibility factor (Z_c), and normal boiling point (T_b) are important constants to check for thermodynamic consistency and to estimate other properties. The n-alkane family is of primary interest because it is generally assumed that other families of compounds behave similarly to …

Characterization, Synthesis And Stabilization Of Au Based Bimetallic Catalysis For The Hydrochlorination Of Acetylene, Kerry Charles O'Connell

Theses and Dissertations

Polyvinyl chloride (PVC) is the third most-produced plastic polymer world-wide by volume after polypropylene and polyethylene. Vinyl chloride monomer, which is polymerized into PVC, is produced via mercuric chloride catalysts that deactivate as mercury sublimes into the atmosphere from the catalyst surface, resulting in substantial environmental concerns.

In an effort to rationally synthesize non-mercuric catalysts, strong electrostatic adsorption (SEA) was used to prepare highly dispersed and active gold nanoparticles over low point of zero charge (PZC) carbon to increase the active metal surface area. The use of <1.5nm gold clusters did not lead to large increases in activity as there was an increased degree of particle coalescence during reaction for activated carbon supported samples. A pronounced support effect was observed in which oxide supports completely prevented Au sintering, but was virtually inactive. This suggests that the active site for the hydrochlorination reaction involves the carbon surface.

The stabilization of carbon supported gold nanoparticles in the HCl-rich reaction environment was …

Adsorption Reversibility Of So2, No2, And No On 13x And 5a Zeolites, Peter Fairchild

Theses and Dissertations

The adsorption reversibility of gasses that are typically found in flue gas for coal based power plants, such as SO2, NO2, and NO, onto 13X and 5A zeolites was presently investigated. The four individual gas mixtures containing these species consisted of 74 ppm NO (balance He), 74 ppm NO2 (balance He), 42 ppm SO2 (balance He), and 500 ppm SO2 (balance N2), which were also consistent with the values that are found in flue gas. Another gas, 15.03 mol% CO2 (balance N2), was also used in this work to evaluate the role of exposing SO2 to the zeolite on their …

Polyphenols As Natural, Dual-Action Therapeutics For Alzheimer's Disease, Kayla M. Pate

Theses and Dissertations

Alzheimer’s disease (AD), the most common form of dementia, is characterized by extracellular plaques in the brain created when monomeric amyloid-β (Aβ) protein aggregates into fibrillar structures. Soluble Aβ aggregates, including oligomers, that form along the reaction pathway are believed to be the primary pathogenic species and have been shown to increase the production of reactive oxygen species (ROS). This upregulation of ROS is one suggested contributing factor of Aβ aggregate cytotoxicity and has proven capable of mediating cell signaling associated with Aβ aggregate-induced cellular responses.

Polyphenols have been suggested as a complimentary AD therapeutic based on epidemiological evidence that …

Development Of Highly Active And Stable Compressive Pt Cathode Catalysts For Polymer Electrolyte Membrane Fuel Cells, Taekeun Kim

Theses and Dissertations

With the limited fossil fuel reserve and increased power demand, polymer electrolyte membrane fuel cells (PEMFC) have been considered to be a promising alternative to the current energy consumption mode due to its high energy conversion, high efficiency, and zero emissions. However, high cost, poor stability, and sluggish kinetic for oxygen reduction reaction (ORR) of Pt/C cathode catalysts are obstacles for the commercialization of PEMFC for automotive application. The observed poor stability is attributed to a corrosion of carbon supports due to low pH, high temperature, and high anodic potentials (1.0-1.5 V) at the cathode interface during start-up/shutdown conditions. Electrochemical …

Development Of Pressure Swing Adsorption (Psa) Processor Co2 Capture From Flue Gas, Md. Atikur Rahman

Theses and Dissertations

Reducing the anthropogenic CO2 emissions and lowering the concentration of greenhouse gases in the atmosphere has become one of the most important environmental issues of recent times. To this end, the development of a cost effective pressure swing adsorption (PSA) process, utilizing commercially available 13X zeolite as the adsorbent, is underway to remove and concentrate CO2 in the flue gas of a coal-fired power plant. This systematic development effort has been carried out so far at the bench scale. It has included the following studies that defined and validated this PSA process both experimentally and via a PSA process model. …

Ultrathin Microporous Metal Oxide Coatings: Preparation By Molecular Layer Deposition, Characterization And Application, Zhuonan Song

Theses and Dissertations

Molecular layer deposition (MLD), a gas phase deposition technique, was applied to deposit conformal organic-inorganic hybrid coatings by conducting a series of sequential, self-limiting surface reactions on substrates with exquisite thickness control at the sub-nanometer level. Obtained organic-inorganic hybrid coatings can subsequently be converted into porous coatings by removing the organic compound. Potential of functional coatings and membranes prepared by MLD was explored for applications from adsorptive separation, water purification, to gas storage.

We demonstrated a new concept, pore misalignment, to continuously fine tune the molecular-sieving “gate” of 5A zeolite by adjusting the external porous Al2O3 MLD coating thickness. For …

Pvdf Membranes With Stable, Ultrathin Graphene Oxide (Go) Functional Coatings For Antifouling Oil/Water Separation Under Cross-Flow Condition, Lei Wang

Theses and Dissertations

Oil/water separation plays an important role in industrial wastewater treatment and environment protection. For the treatment of oily wastewater, especially for removing oil droplets with sizes in the micrometer range, ultrafiltration (UF) membrane technology is considered the most efficient method due to its high separation efficiency and relatively simple operational process. However, caused by the deposition of the oil droplets onto the membrane surface and into the membrane pores, the fouling of UF membranes shortens their service time and degrades their separation performance in practical applications.

In this work, graphene oxide (GO) was utilized as a novel coating material to …

Dynamic Liquefied Natural Gas (Lng) Processing With Energy Storage Applications, Farhad Fazlollahi

Theses and Dissertations

The cryogenic carbon capture™ (CCC) process provides energy- and cost-efficient carbon capture and can be configured to provide an energy storage system using an open-loop natural gas (NG) refrigeration system, which is called energy storing cryogenic carbon capture (CCC-ES™). This investigation focuses on the transient operation and especially on the dynamic response of this energy storage system and explores its efficiency, effectiveness, design, and operation. This investigation included four tasks.The first task explores the steady-state design of four different natural gas liquefaction processes simulated by Aspen HYSYS. These processes differ from traditional LNG process in that the CCC process vaporizes …

Thermal And Convective Loading Methods For Releasing Hydrophobic Therapeutics From Contact Lenses, Ryan Ruben Horne

Theses and Dissertations

This thesis investigates the feasibility of loading silicone hydrogel (SiHy) contact lenses with two different hydrophobic therapeutics, latanoprost and DMPC (1,2-dimyristoyl-sn-glycero-3-phosphocholine), for treatment of glaucoma and hyperemia respectively. The two methods of loading were 1) thermal loading in an aqueous medium and 2) convective loading in a solution of n-propanol. Dailies Total1® lenses prepared in this manner were tested for their loading and their release into artificial tears. Continuous release over 1-4 days at therapeutic levels is achievable from thermal loading of DMPC, convective loading of DMPC, and convective loading of latanoprost. The DMPC loading processes can be naturally integrated …

Nerve Regeneration Using Lysophosphatidylcholine And Nerve Growth Factor, Ryan Lavar Wood

Theses and Dissertations

Peripheral nerve damage affects hundreds of thousands of people every year. This study tested the effectiveness of using lysophosphatidylcholine (LPC) in combination with nerve growth factor (NGF) to increase the healing rate of damaged left sciatic nerves in female rats. The rats were randomly divided into eight groups: Sham, Right Sciatic, Crush, LPC, LPC-NGF, Crush- LPC, Crush-NGF, and Crush-LPC-NGF. The healing of the nerves was measured by monitoring gait, electrophysiological parameters (compound muscle action potential amplitudes and nerve conductance velocities) and morphological parameters (total fascicular area, total myelinated fiber counts, fiber densities, fiber diameters, and g-ratio). Gait and electrophysiological parameters …

Developing Modeling, Optimization, And Advanced Process Control Frameworks For Improving The Performance Of Transient Energy-Intensive Applications, Seyed Mostafa Safdarnejad

Theses and Dissertations

The increasing trend of world-wide energy consumption emphasizes the importance of ongoing optimization of new and existing technologies. In this dissertation, two energy–intensive systems are simulated and optimized. Advanced estimation, optimization, and control techniques such as a moving horizon estimator and a model predictive controller are developed to enhance the profitability, product quality, and reliability of the systems. An enabling development is presented for the solution of complex dynamic optimization problems. The strategy involves an initialization approach to large–scale system models that both enhance the computational performance as well as the ability of the solver to converge to an optimal …

An Analysis Of Capital Cost Estimation Techniques For Chemical Processing, Omar Joel Symister

Theses and Dissertations

This research serves to compare the use of the capital cost estimation software, Aspen Capital Cost Estimator (ACCE), with other capital cost estimating methods specifically the module costing technique outlined by Richard Turton et al. and also a factorial costing technique outlined by Gavin Towler and Ray Sinnott. This study will compare popular process equipment found in the chemical process industries. The relationship between the capacities of the equipment, as it relates to the cost as well as operational pressures and materials of construction (MOC), will also be obtained and compared. The results of this study may be used by …

Mitigating Transients And Azeotropes During Natural Gas Processing, Edris Ebrahimzadeh

Theses and Dissertations

Cryogenic carbon capture process can be used to efficiently eliminate CO2 emissions from fossil-fueled power plants. The energy-storing embodiment of cryogenic carbon capture (ES-CCC) integrates energy storage with cryogenic carbon capture and uses natural gas as a refrigerant. ES-CCC captures CO2 from slowly varying or steady-state sources even as it absorbs and replaces large amounts of energy on the grid for energy storage. These large transients occur in the LNG generation as the process moves through energy storing to energy recovery operations. Additionally, raw natural gas often includes CO2 that forms an azeotrope with ethane. Breaking this azeotrope and separating …

Aminoacyl-Trna Synthetase Production For Unnatural Amino Acid Incorporation And Preservation Of Linear Expression Templates In Cell-Free Protein Synthesis Reactions, Andrew Broadbent

Theses and Dissertations

Proteins—polymers of amino acids—are a major class of biomolecules whose myriad functions facilitate many crucial biological processes. Accordingly, human control over these biological processes depends upon the ability to study, produce, and modify proteins. One innovative tool for accomplishing these aims is cell-free protein synthesis (CFPS). This technique, rather than using living cells to make protein, simply extracts the cells' natural protein-making machinery and then uses it to produce protein in vitro. Because living cells are no longer involved, scientists can freely adapt the protein production environment in ways not otherwise possible. However, improved versatility and yield of CFPS protein …

Extracellular Matrix From Whole Porcine Heart Decellularization For Cardiac Tissue Engineering, Nima Momtahan

Theses and Dissertations

Heart failure is one of the leading causes of death in the United States. Every year in the United States, more than 800,000 people are diagnosed with heart failure and more than 375,000 people die from heart disease. Current therapies such as heart transplants and bioartificial hearts are helpful, but not optimal. Decellularization of porcine whole hearts followed by recellularization with patient-specific human cells may provide the ultimate solution for patients with heart failure. Great progress has been made in the development of efficient processes for decellularization, and the design of automated bioreactors. In this study, the decellularization of porcine …

The Influence Of Season, Heating Mode And Slope Angle On Wildland Fire Behavior, Jonathan R. Gallacher

Theses and Dissertations

Wildland fire behavior research in the last 100 years has largely focused on understanding the physical phenomena behind fire spread and on developing models that can predict fire behavior. Research advances in the areas of live-fuel combustion and combustion modeling have highlighted several weaknesses in the current approach to fire research. Some of those areas include poor characterization of solid fuels in combustion modeling, a lack of understanding of the dominant heat transfer mechanisms in fire spread, a lack of understanding regarding the theory of live-fuel combustion, and a lack of understanding regarding the behavior of flames near slopes. In …

Sustainable P2 Design For Batch-Based Specialty Chemical Manufacture, Brigitte Pastore

Theses and Dissertations

A case study has been conducted on the reduction of n-methyl-2-pyrrilodone (NMP) solvent waste in the manufacture of polyimide and polybenzoxazole precursors. The evaluation includes the environmental and economic assessment of solvent recovery and substitution strategies. A two-step distillation process proved effective in recovering 95% of the NMP at a purity of 99.97% from the aqueous solvent waste stream. Yearly operating costs were reduced by 83% and total life cycle emissions were reduced by 44%, due to reduction in virgin NMP use and hazardous waste disposal. With capital acquisition, the recovery option would still result in a net present value …

Design And Development Of A High-Temperature High-Pressure Rolling Ball Viscometer/Densimeter And Evaluation Of Star Polymer-Solvent Mixtures, Matthew Stanton Newkirk

Theses and Dissertations

Modern automotive applications such as transmission clutch plates, combustion chambers, diesel fuel injector tips, and axle gears and friction plates operate at temperatures that can exceed 250°C and pressures of 40,000 psia. Industrial practice is to add homopolymers and copolymers to base oils to modify bulk fluid viscosity and frictional properties for these demanding applications. However, designing polymeric additives for lubricants and predicting their performance is limited by the lack of available high-temperature high-pressure (HTHP) viscosity and density data needed to test contemporary lubricity models. Thus, a major objective of this thesis is the design, development, and commissioning of a …

Synthesis Of Well Dispersed Supported Metal Catalysts By Strong Electrostatic Adsorption And Electroless Deposition, John Meynard Macasero Tengco

Theses and Dissertations

Enhancing the catalytic performance of heterogeneous catalysts can be done by increasing active site count as well as modification of the physico-chemical characteristics of the catalyst material. For supported metal nanoparticles this can be achieved by decreasing particle size, thus increasing dispersion or metal utilization on the surface of the particles, while modification of metal properties can be attained by addition of a secondary metal that has a strong interaction to the primary metal, beneficial for a given reaction. In addition, the accessibility of the metal surface is necessary for reliable performance of catalysts. For carbon supported catalysts, Temperature Programmed …

Development Of A Pressure Swing Adsorption (Psa) Cycle For Co2 Capture From Flue Gas Using A 4-Bed Psa Apparatus, Joshua White

Theses and Dissertations

Pressure swing adsorption is an effective way to capture CO2 from flue gases in energy plants. Pressure swing adsorption utilizes an adsorbent’s selectivity to a specific compound to remove it from a feed stream. Zeolite 13X was used for this research to obtain a high purity and recovery of CO2 at a higher feed flow than previous research. The first part of the work was to use in house FORTRAN based Dynamic Adsorption Process Simulator (DAPS) that uses the finite difference method and the time adaptive Differential- Algebraic Equation (DAE) solver called DASPK to narrow down appropriate experiment criteria for …

Rational Design And Synthesis Of Pt/Silica-Alumina Metal-Acid Bifunctional Catalysts, Jadid Ettaz Samad

Theses and Dissertations

In the domain of rational catalyst design and synthesis, Strong Electrostatic Adsorption (SEA) has been applied with tremendous potential and use. In this study, SEA method has been advanced to thick slurries as well as to mixed oxide systems with a view to synthesizing stable, active and selective catalysts fine-tuned for specific applications.

The main objective was to synthesize metal-acid bifunctional catalysts with controlled ratio and proximity between metal and acid sites in order to analyze their effects on a bifunctional reaction. At first, the difference in charging behavior of silica and alumina in solution was used to selectively deposit …

Biodegradable Hybrid Tissue Engineering Scaffolds For Reconstruction Of Large Bone Defects, Danial Barati

Theses and Dissertations

Complex skeletal injuries and large bone fractures are still a significant clinical problem in US. Approximately 1.5 million Americans (veterans, their families, and civilians) every year suffer from bone loss due to traumatic skeletal injuries, infection, and resection of primary tumors that require extensive grafting to bridge the gap. The US bone graft market is over $2.2 billion a year. Due to insufficient mechanical stability, lack of vascularity, and inadequate resorption of the graft, patients with traumatic large skeletal injuries undergo multiple costly operations followed by extensive recovery steps to maintain proper bone alignment and length. Current strategies for repairing …

Solving Atomic Structures Using Statistical Mechanical Searches On X-Ray Scattering Derived Potential Energy Surfaces, Christopher James Wright

Theses and Dissertations

Engineering the next generation of materials, especially nanomaterials, requires a detailed understanding of the material’s underlying atomic structure. These structures give us better insight into structure-property relationships, allowing for property driven material design on the atomic level. Even more importantly, understanding structures in-situ will translate stimuli and responses on the macroscopic scale to changes on the nanoscale. Despite the importance of precise atomic structures for materials design, solving atomic structures is difficult both experimentally and computationally. Atomic pair distribution functions (PDFs) provide information on atomic structure, but the difficulty of extracting the PDF from x-ray total scattering measurements limits their …

Methane Separation And Purification Via Pressure Swing Adsorption, Lutfi Erden

Theses and Dissertations

Investigations on alternative energy sources have gained increasing attention due to significant shortage in the fossil fuel reserves and increase on the usage of internal combustion engines. Natural gas which mainly consists of Methane has a significant role in short term solution of this issue. Although methane as a greenhouse gas is over 20 times more potent by weight than carbon dioxide, if released directly into the atmosphere, it is a valuable energy source. However, methane is not extracted % 100 pure from the source. Natural gas generally contains many other contaminants such as water, other hydrocarbons, Carbon dioxide, Nitrogen, …

Mathematical Modeling Of Transport And Corrosion Phenomenon Inside High Temperature Molten Salt Systems, Bahareh Alsadat Tavakoli Mehrabadi

Theses and Dissertations

In today's world, alternative clean methods of energy are needed to meet growing energy demands. Concentrating solar thermal power, more commonly referred to as CSP, is unique among renewable energy generators because even though it is variable, like solar photovoltaic and wind, it can easily be coupled with thermal energy storage as well as conventional fuels, making it highly dispatchable. One challenge with concentrated solar power (CSP) systems is the potential corrosion of the alloys in the receivers and heat exchangers at high-temperature (700-1000 °C), which leads to a reduction of heat transfer efficiency and influences the systems durability. The …

Development Of Novel High-Throughput Methodologies To Evaluate The Thermal Stability Of High-Temperature Thin-Film Crystals For Energy Applications, Jonathan Kenneth Bunn

Theses and Dissertations

High temperature materials are used in a variety of energy applications such as jet turbines, high temperature sensors, and solid oxide fuel cells (SOFCs). Discovery of new materials and their optimization represents a difficult engineering challenge, as the materials have to be designed to have a high figure of merit during intense high temperature operations.

The large number of engineering considerations involved in the identification and optimization of such materials makes traditional one-by-one material investigations prohibitively time consuming. The time needed to explore large parameter domains can be significantly decreased through the use of high-throughput experimentation (HTE). HTE is an …

The Oxidation And Decoration Chemistry Of Platinum And Palladium Nanoparticles On Carbon Supports, Ritubarna Banerjee

Theses and Dissertations

Ultrasmall platinum and palladium nanoparticles supported on carbon are used in a wide variety of industrial catalytic processes including hydrogenation-dehydrogenation reactions, isomerization of hydrocarbons, ammonia and formic acid decomposition, the oxidation of carbon monoxide, alcohols and ammonia, and fuel cells. This dissertation covers three aspects of the chemistry of these ultrasmall, carbon supported nanoparticles.

In the first vein of work, the oft-observed discrepancy in Pd nanoparticle size estimation between chemisorption and other methods such as STEM and XRD is explored. It is demonstrated that lower-than-expected chemisorption uptake can stem from not only residual chloride, but also from the decoration of …