Engineering Commons^™

Multi-Criteria Decision Matrix Method In The Risk Analysis Of Biodiesel Production Processes, Kaylee Alles

Department of Chemical and Biomolecular Engineering: Theses and Student Research

Renewable fuel technologies aim to mitigate the non-renewability of fossil fuels, challenges with increased energy demand, and the climate impact of fossil fuel emissions. However, before investing in renewable technologies, there need to be decision strategies that assess and identify the best alternatives according to stakeholder priorities. There is also a concern about whether the technologies that are the “most sustainable” effectively meet the acceptable risk requirements of stakeholders. In response to this question, a risk-adapted multi-criteria decision model was developed and compared to a sustainability study that evaluated five renewable diesel technologies, including Green Diesel I, II, and III; …

Complete Modeling Of The Chondrogenic Environment Under Continuous Low-Intensity Ultrasound, Heather Newell

Department of Chemical and Biomolecular Engineering: Theses and Student Research

Articular cartilage is an avascular tissue that requires therapeutic intervention methods. This work answers the following: determine transducer operation to optimize the bioeffects; calculate the magnitude of pressure exerted on chondrocytes at an injury site; and confirm the theoretical findings by an animal model.

Earlier work has shown that cellular response to US is maximized at the resonance frequency of the cells. Resonance frequencies were calculated for chondrocytes in various layers. The latter configuration closest resembles in vivo conditions and the resonance occurred at 3.8±0.3 𝑀𝐻𝑧. The 3D model of US propagation in a rabbit knee was constructed from MRIs …

Finite Element Modelling And Exergy Analysis Of A Solar Thermal Hollow Fiber Vacuum Membrane Distillation System, Benjamin Shuldes

Department of Chemical and Biomolecular Engineering: Theses and Student Research

A finite element model was developed to investigate the performance of a vacuum membrane distillation module under various operating conditions and membrane parameters. Porosity, tortuosity, pore diameter, membrane thickness, and fiber length were varied along with feed temperature, velocity, and flow configuration. In all cases, boundary layer polarization phenomena were seen to inhibit the performance of the module. At certain conditions, for a 7.5 cm fiber, the reduction in permeate flux from 65 LMH (Liter/m²/h) at the inlet to below 45 LMH at the outlet of the fibers was observed. In most cases, salt concentration polarization was the …

Metabolic Modeling And Omics-Integrative Analysis Of Single And Multi-Organism Systems: Discovery And Redesign, Mohammad Mazharul Islam

Department of Chemical and Biomolecular Engineering: Theses and Student Research

Computations and modeling have emerged as indispensable tools that drive the process of understanding, discovery, and redesign of biological systems. With the accelerating pace of genome sequencing and annotation information generation, the development of computational pipelines for the rapid reconstruction of high-quality genome-scale metabolic networks has received significant attention. These models provide a rich tapestry for computational tools to quantitatively assess the metabolic phenotypes for various systems-level studies and to develop engineering interventions at the DNA, RNA, or enzymatic level by careful tuning in the biophysical modeling frameworks. in silico genome-scale metabolic modeling algorithms based on the concept of optimization, …

Creation And Application Of Various Tools For The Reconstruction, Curation, And Analysis Of Genome-Scale Models Of Metabolism, Wheaton L. Schroeder

Department of Chemical and Biomolecular Engineering: Theses and Student Research

Systems biology uses mathematics tools, modeling, and analysis for holistic understanding and design of biological systems, allowing the investigation of metabolism and the generation of actionable hypotheses based on model analyses. Detailed here are several systems biology tools for model reconstruction, curation, analysis, and application through synthetic biology. The first, OptFill, is a holistic (whole model) and conservative (minimizing change) tool to aid in genome-scale model (GSM) reconstructions by filling metabolic gaps caused by lack of system knowledge. This is accomplished through Mixed Integer Linear Programming (MILP), one step of which may also be independently used as an additional curation …

Inducing Increased Bioplastic Production In R. Palustris Cga009, Cameron Gilley, Dylan Hoppner, Brandi J. Brown

Department of Chemical and Biomolecular Engineering: Theses and Student Research

PHA’s (polyhydroxyalkanoates) are important bio polymers in different industries such as petroleum, medicine, and nano technology. In the microorganisms in which they are produced, they serve as an energy storage material by storing both carbon and usable electrons. This is useful in environments where the organisms are nutrient starved. PHA’s have a practical use especially in the medical field as bio-plastics because they are biodegradable and bio-compatible. Rhodopseudomonas. palustris, a common soil bacterium, is notable for its uncommon metabolic flexibility. Its diverse metabolism means that it can fix CO 2 and grow on many lignin based monomers in both aerobic …

Fabrication Of Polyvinylidene Fluoride Hollow Fiber Membranes For Membrane Distillation, Abdullah Al Balushi

Department of Chemical and Biomolecular Engineering: Theses and Student Research

Desalination technologies can help humanity tap into the most abundant source of water on earth, seawater; however, desalination is an energy-demanding process. Most of the desalination plants worldwide use conventional energy resources; therefore, desalination leaves a large carbon footprint. Solar energy is an available source of energy that can be harvested and integrated into desalination systems.

Membrane distillation (MD) is an emerging purification technology that many offers many advantages over traditional desalination systems. For starters, it can utilize low-grade thermal energy to drive the separation, therefore, it can be suitably integrated into the solar-thermal energy scheme. Additionally, MD can be …

Bioproduction Of Adipic Acid Using Engineered Pseudomonas Putida Kt2440 From Lignin-Derived Aromatics, Howard Willett

Department of Chemical and Biomolecular Engineering: Theses and Student Research

Current industrial synthesis of adipic acid is nonrenewable and depends on a carcinogenic starting material, benzene. Biocatalysis with an engineered microorganism could turn a renewable feedstock into a value-added chemical such as adipic acid. Here we engineered P. putida KT2440 to transform lignin-derived aromatics, coumarate and ferulate, into adipic acid. Lignin is a recalcitrant plant biopolymer burned for thermal energy. Conversion of lignin into a value-added chemical will improve the efficiency of lignocellulose processing plants. The best performing engineered KT2440 strain produces 2.52 mM adipate at a 9.5% (mole/mole) yield. This was achieved by the genetic insertion of non-natural biosynthetic …

Modeling The Interplay Between Photosynthesis, Co2 Fixation, And The Quinone Pool In A Purple Non-Sulfur Bacterium, Adil Alsiyabi

Department of Chemical and Biomolecular Engineering: Theses and Student Research

Rhodopseudomonas palustris CGA009 is a purple non-sulfur bacterium (PNSB) that can fix CO₂ and nitrogen or break down organic compounds for its carbon and nitrogen requirements. Light, inorganic, and organic compounds can all be used for its source of energy. Excess electrons produced during its metabolic processes can be exploited to produce hydrogen gas or biodegradable polyesters (polyhydroxybutyrate). A genome-scale metabolic model of the bacterium was reconstructed to study the interactions between photosynthesis, carbon dioxide fixation, and the redox state of the quinone pool. A comparison of model-predicted flux values with published in vivo MFA fluxes resulted in predicted …

Fibrinogen, Factor Xiii And Fibronectin: A Biophysical And Kinetic Characterization Of Their Interactions, Frank Fabian

Department of Chemical and Biomolecular Engineering: Theses and Student Research

The development of recombinant-based liquid fibrin tissue sealants having enhanced hemostatic and wound healing properties will involve understanding as yet not well characterized interactions between fibrinogen, fibrin (Fbn) factor XIII, thrombin and fibronectin. We study these phenomena in the context of comparing plasma derived fibrinogen to recombinant fibrinogen (rFI) produced in the milk of transgenic cows. An abundance of purified γγ and γγ’ FI subspecies enables detailed study of γγ or γγ’ biomonomer and their respective Fbn biopolymer formation as having different substrate behaviors of activated plasma derived factor XIII (pFXIIIa₂b₂). High pressure size exclusion (HPSEC) …

Sustainable Clean Coal Technology With Power And Methanol Production, Xiaomeng Wang

Department of Chemical and Biomolecular Engineering: Theses and Student Research

The coal-based chemical process is still indispensable in modern society due to the worldwide vast reserves and popular price of coal. Power generation and chemical production from coal still play an important role in the global chemical industrial market. Electricity generation and chemical production from coal is still the trend as long as the coal is plentiful and inexpensive. Modern chemical industry aims at sustainability and hence the development of clean coal technologies is critical.

Coal-based methanol economy, as an attractive liquid transportation fuel as well as an essential intermediate chemical feedstock, can fill a possible gap between declining fossil …

Sustainability Assessment For Energy Systems And Chemical Process Industries, Michael J. Matzen

Department of Chemical and Biomolecular Engineering: Theses and Student Research

Sustainability has become an important factor in the chemical process and energy industries with a strong drive for process improvements towards more environmentally conscious solutions. However, there are many ways of defining sustainability and even more ways of trying to determine how sustainable a process is. This work looks into applying a conjunction of tools including; process simulation, multi-criteria decision matrices and life-cycle assessment to more quantitatively determine sustainability metrics. We have applied these tools for the production of electricity, methanol and dimethyl ether. A novel method of electricity production, in chemical looping combustion (CLC), was used that inherently involves …

Economic And Sustainability Aspects Of Chemical Process Industries By Thermodynamics Analysis, Mahdi H. Alhajji

Department of Chemical and Biomolecular Engineering: Theses and Student Research

Economics and sustainability improvements of some chemical processes are assessed after the retrofits suggested by thermodynamic analysis and energy analyzer. The main objective is to explore the scope of reducing the energy consumption and CO₂ emissions for a more sustainable operation in chemical process industries. Thermodynamic analysis is carried out by applying the thermal analysis capability of ‘Column Targeting Tool’ to address the ‘energy intensity,’ and/or the ‘Energy Analyzer’ to design and improve the performance of the heat exchanger network system for process heat integration. CTT is based on the ‘Practical Near-Minimum Thermodynamic Condition’ representing a close to practical …

Titanium Dioxide Nanoparticles Trigger Loss Of Hepatic Function And Perturbation Of Mitochondrial Dynamics In Primary Hepatocytes, Vaishaali Natarajan

Department of Chemical and Biomolecular Engineering: Theses and Student Research

Titanium dioxide (TiO₂) nanoparticles are one of the most highly manufactured nanomaterials in the world with applications in copious industrial and consumer products. The liver is a major accumulation site for many nanoparticles, including TiO₂, directly through intentional ingestion or indirectly through increased environmental contamination and unintentional ingestion via water, food or animals. Growing concerns over the current usage of TiO₂ coupled with the lack of mechanistic understanding of its potential health risk is the motivation for this study. Here we determined the toxic effect of three different TiO₂ nanoparticles (commercially available rutile, anatase …

Engineering Of Lipid Nanoparticles For Advanced Drug Delivery Applications, Stephen L. Hayward

Department of Chemical and Biomolecular Engineering: Theses and Student Research

‘Nanomedicine’, the application of nanotechnology principles to the field of medicine, has stimulated the development of nano-platforms for next generation drug delivery. By exploiting nanoscale properties of materials to selectively alter intrinsic characteristics of therapeutics, researchers have improved the efficacy and pharmacokinetic profiles for a variety of drug types. Despite preliminary commercial and clinical success, there still remains a need to develop an improved delivery platform that can provide high cargo entrapment, efficient intracellular delivery, evasion of intracellular degradation pathways, and provide cell population specific targeting.

In this study we engineered a nanocarrier system composed of a core bilayer structure …

Low Molecular Weight Glucosamine/L-Lactide Copolymers As Potential Carriers For The Development Of A Sustained Rifampicin Release System: Mycobacterium Smegmatis As A Tuberculosis Model, Jorge Ragusa

Department of Chemical and Biomolecular Engineering: Theses and Student Research

Tuberculosis, a highly contagious disease, ranks as the second leading cause of death from an infectious disease, and remains a major global health problem. In 2013, 9 million new cases were diagnosed and 1.5 million people died worldwide from tuberculosis. This dissertation aims at developing a new, ultrafine particle-based efficient antibiotic delivery system for the treatment of tuberculosis. The carrier material to make the rifampicin (RIF)-loaded particles is a low molecular weight star-shaped polymer produced from glucosamine (molecular core building unit) and L-lactide (GluN-LLA). Stable particles with a very high 50% drug loading capacity were made via electrohydrodynamic atomization. Prolonged …

Production Of Recombinant Human Coagulation Factor Ix By Transgenic Pig, Weijie Xu

Department of Chemical and Biomolecular Engineering: Theses and Student Research

Hemophilia B is the congenital bleeding disorder caused by deficiency in functional coagulation factor IX (FIX) and about 28,000 patients worldwide in 2012. And current treatment is restricted to protein-replacement therapy, which required FIX concentrates for patients’ life-time. Approximately 1 billion units FIX were consumed in 2012. However, still about 70-80% patients, mostly in developing countries, received inadequate or no treatment because of the unavailable and/or unaffordable FIX concentrates. Considering safety reasons, e.g. transmission of blood-borne diseases, the recombinant human FIX (rFIX) is recommended other than the plasma-derived FIX. However, only one rFIX is currently available on the market. The …

Opto-Electronic Devices With Nanoparticles And Their Assemblies, Chieu Van Nguyen

Department of Chemical and Biomolecular Engineering: Theses and Student Research

Nanotechnology is a fast growing field; engineering matters at the nano-meter scale. A key nanomaterial is nanoparticles (NPs). These sub-wavelength (< 100nm) particles provide tremendous possibilities due to their unique electrical, optical, and mechanical properties. Plethora of NPs with various chemical composition, size and shape has been synthesized. Clever designs of sub-wavelength structures enable observation of unusual properties of materials, and have led to new areas of research such as metamaterials. This dissertation describes two self-assemblies of gold nanoparticles, leading to an ultra-soft thin film and multi-functional single electron device at room temperature. First, the layer-by-layer self-assembly of 10nm Au nanoparticles and polyelectrolytes is shown to behave like a cellular-foam with modulus below 100 kPa. As a result, the composite thin film (~ 100nm) is 5 orders of magnitude softer than an equally thin typical polymer film. The thin film can be compressed reversibly to 60% strain. The extraordinarily low modulus and high compressibility are advantageous in pressure sensing applications. The unique mechanical properties of the composite film lead to development of an ultra-sensitive tactile imaging device capable of screening for breast cancer. On par with human finger sensitivity, the tactile device can detect a 5mm imbedded object up to 20mm below the surface with low background noise. The second device is based on a one-dimensional (1-D) self-directed self-assembly of Au NPs mediated by dielectric materials. Depending on the coverage density of the Au NPs assembly deposited on the device, electronic emission was observed at ultra-low bias of 40V, leading to low-power plasma generation in air at atmospheric pressure. Light emitted from the plasma is apparent to the naked eyes. Similarly, 1-D self-assembly of Au NPs mediated by iron oxide was fabricated and exhibits ferro-magnetic behavior. The multi-functional 1-D self-assembly of Au NPs has great potential in modern electronics such as solid state lighting, plasma-based nanoelectronics, and memory devices.

Adviser: Ravi F. Saraf

Pharmacokinetic Characterization Of Procoagulation Proteins, Nicholas C. Vanderslice

Department of Chemical and Biomolecular Engineering: Theses and Student Research

The cessation of bleeding in mammals occurs due to a well-conserved sequence of protein activation known as the coagulation cascade. However, people who have a deficiency in one or more proteins in this cascade, whether due to genetics or blood loss, struggle to maintain hemostasis. In order to aid patients in the restoration of hemostasis, exogenous proteins are often administered in response to bleeding events. However, these proteins are limited and costly due to limited supply of donor blood available for the production of plasma-derived proteins and the high cost of mammalian cell bio-reactors required for the production of recombinant …

Development And Utilization Of A Pair Of Sol-Gel Entrapped Lipases For Biodiesel Production From High Free Fatty Acid Oils, Cory M. Schwartz

Department of Chemical and Biomolecular Engineering: Theses and Student Research

Biodiesel, which consists of fatty acid alkyl esters, is one of the most widely adopted and successful renewable fuels. Traditional physiochemical biodiesel production methods require high cost refined feedstocks, and so alternative methods of catalysis and feedstocks have been explored. This research investigated the use of polysiloxane entrapped lipases to catalyze the production of biodiesel from low cost feedstocks.

In this work, lipase from Burkholderia cepacia (lipase PS) and lipase B from Candida antarctica (CalB) were separately entrapped using sol-gel chemistry. Optimal reaction conditions for the esterification of free fatty acids by immobilized CalB with methanol were determined. Immobilized CalB …

Utilization Of Wet Corn Distillers Grain For The Production Of Cellulase Enzymes And Value Added Products, Hunter R. Flodman

Department of Chemical and Biomolecular Engineering: Theses and Student Research

Distillers grain, a byproduct from the dry grind corn ethanol industry, is an industrially available, abundant, preprocessed material comprised primarily of cellulose, hemicellulose, protein, oil, and minerals. This research investigated the utilization of distillers grain as a substrate for solid-state fermentation (SSF). SSF is the growth of microorganisms on a moist, solid substrate in the absence of free water. The substrate acts as both the nutrient source and as a solid support for the microorganisms, typically filamentous fungi. SSF has the unique ability to convert agricultural byproducts into valuable bioproducts such as enzymes and organic acids without hydrolyzing polysaccharides to …

Optimization Of Biodiesel Production Plants, Nghi T. Nguyen

Department of Chemical and Biomolecular Engineering: Theses and Student Research

A conventional biodiesel plant utilizing two distillation columns to purify unreacted reactants and products is considered in this study. Thermodynamic analyses are used to assess the performance of the existing distillation columns, and reduce the costs of operation by appropriate retrofits in a biodiesel production plant. After the retrofits, the overall exergy loss for the two columns has decreased from 2430.87 kW to 1674.12 kW.

A reactive distillation is developed for esterification of lauric acid with methanol using equilibrium and nonequilibrium models. Equilibrium modeling dominated during last few decades due to their straightforward mathematical modeling. In reality, separation depends on …

Fabrication And Characterization Of Thermomechanically Processed Sulfur And Boron Doped Amorphous Carbon Films, Lonnie Carlson

Department of Chemical and Biomolecular Engineering: Theses and Student Research

Small scale, high power density, reliable, and long-life power supplies would be useful or even critical for space missions or the growing number of microdetectors, microsensors, and miniature vehicles. Alpha or beta particle voltaic devices could satisfy these requirements but have been shown to degrade quickly due to radiation damage. Amorphous carbon (a-C) PN junctions or PIN devices could provide radiation hardness and sufficiently high efficiency. As the range of alpha and beta particles in a-C is ~20-120μm, much thicker films than are typical are needed to maximize collection of the particle energy.

In this work, the fabrication of thermomechanically …

Purification Of Fibrinogen From Human Plasma, Ayman E. Ismail

Department of Chemical and Biomolecular Engineering: Theses and Student Research

A solvent detergent treated fibrinogen was purified from human plasma by cryoprecipitation (cryo) followed by chemical precipitation using ethanol (EtOH) or ammonium sulfate (AS) as precipitating agents. Amounts of fibronectin (FN), factor XIII A-subunit (FXIIIA), factor XIII b-subunit (FXIIIB), and alpha₂-antiplasmin (α₂-AP) in the isolated fibrinogen were quantified. Thromboelastography (TEG) analysis was used to evaluate the clot strength of the isolated fibrinogen and to determine the ability of the ethanol and ammonium sulfate precipitations to eliminate the solvent detergent. Sodium dodecylsulfate-polyacrylamide gel analysis indicated that fibrinogen produced by each of these precipitation methods had similar purity. …

Technological Aspects Of Molecular Diagnosis Of Bacterial Infectious Diseases, Christine S. Booth

Department of Chemical and Biomolecular Engineering: Theses and Student Research

The polymerase chain reaction (PCR) is continually growing in its application, particularly in the field of molecular diagnosis of disease from clinical specimens. The main focus has been in the detection and identification of pathogens. However, quantitative PCR is increasingly utilized to determine initial pathogen load. A well-designed PCR protocol is required in all of these instances. Just as importantly, in the context of disease diagnosis; is the design of the sample processing methodology. The ideal method should concentrate the DNA and effectively isolate a high-quality DNA product, free of PCR inhibitors, while also being simple, reproducible and safe.

The …

Real-Time Characterization Of Ultra-Thin Organic Layers Via Simultaneous Spectroscopic Ellipsometry And Piezoelectric Nanogravimetry, Keith B. Rodenhausen Jr.

Department of Chemical and Biomolecular Engineering: Theses and Student Research

Analysis techniques are needed to determine the quantity and structure of materials composing an organic layer that is below an optical ultra-thin film limit and in a liquid environment. Neither optical nor acoustical techniques can independently distinguish between thickness and porosity of ultra-thin films due to parameter correlation. A combined optical and acoustical approach yields sufficient information to determine both thickness and porosity. The author describes application of the combinatorial approach to measure single or multiple organic layers when the total layer thickness is small compared to the wavelength of the probing light. The instrumental setup allows for simultaneous in-situ …

Nanoparticle Necklace Network Arrays Exhibiting Room Temperature Single-Electron Switching, Jennifer L. Kane

Department of Chemical and Biomolecular Engineering: Theses and Student Research

A single nanoparticle is one of the most sensitive electronic devices for sensing chemicals in a gas or liquid. The conductivity of a single Au nanoparticle is significantly modulated by the binding of a molecule that alters charge by just one electron. However, the single-electron sensitivity requires cryogenic temperatures and interconnection is not easy. A patterned two-dimensional network of one-dimensional nanoparticle necklaces made up of 10 nm Au particles are fabricated and shown to exhibit similar single-electron effect at room temperature. Furthermore, the long range conductivity of over 10’s of microns makes the structure easy to self-assemble onto conventional microelectronics …

Expression And Characterization Of Protein Chimera With Embedded Activated Protein C Generation, Minjeong Schneider

Department of Chemical and Biomolecular Engineering: Theses and Student Research

Increasing demand of medical implants has lead researchers to develop biomaterial surfaces that offer improved performance and lower thrombogenecity. There have been attempts to model biomaterial surfaces after the native endothelium as it represents an optimal non-thrombotic surface. Typically, when biomaterials are contacted with blood, they often invoke the activation of clotting cascades. Thus, to overcome surface mediated thrombotic events, proteins and molecules with anticoagulant attributes of the endothelium have been immobilized onto biomaterial surfaces. Protein C (PC) plays a main role in blood coagulation and acts as an anticoagulant when it is converted into activated protein C (APC). Activation …

De Novo Gene Synthesis By Rapid Polymerase Chain Assembly Coupled With Immunoaffinity Purification: A Novel Process And Workstation, Joel R. Termaat

Department of Chemical and Biomolecular Engineering: Theses and Student Research

The de novo synthesis of genes is emerging as a powerful tool in biotechnology. The ability to synthesize genes of any desired sequence opens the door to seemingly unlimited research possibilities. Major advances have been made recently in de novo gene synthesis, with Polymerase Chain Assembly (PCA) routinely used to construct functional sequences from short single-stranded oligonucleotides. However, current PCA techniques are lacking in speed and fidelity. Additionally, substantial undesired reactants/products are present in the final reaction. A novel process and accompanying workstation has been developed that incorporates rapid PCA synthesis coupled with subsequent affinity purification of the synthesis mixture. …

An Integrated Approach For Phytate Degradation And Recovery Of Myo-Inositol And Phosphate As Valued-Added Products From The By-Products Of Corn Ethanol Industry, Jun Dang

Department of Chemical and Biomolecular Engineering: Theses and Student Research

An integrated process was developed to hydrolyze the phytates in light steep water (LSW) and to simultaneously isolate inorganic phosphate (Pi) and myo-inositol products. The proposed integrated process is helpful in resolving the environmental and nutritional concerns in the use of corn gluten feed (CGF) in the animal diets. This process comprised of partial and total hydrolysis of LSW and intermediate anion exchange separation technique. The phytates in LSW were initially degraded to negatively charged myo-inositol phosphates (InsP₂ - InsP₅). The optimized experimental parameters for the partial hydrolysis of LSW were determined to be 2 h hydrolysis …