Engineering Commons^™

Resolution Limit For Dna Barcodes In The Odijk Regime, Yanwei Wang, Wes F. Reinhart, Douglas R. Tree, Kevin D. Dorfman

Faculty Publications

We develop an approximation for the probability of optically resolving two fluorescent labels on the backbone of a DNA molecule confined in a nanochannel in the Odijk regime as a function of the fluorescence wavelength, channel size, and the properties of the DNA (persistence length and effective width). The theoretical predictions agree well with equivalent data produced by Monte Carlo simulations of a touching wormlike bead model of DNA in a high ionic strength buffer. Although the theory is only strictly valid in the limit where the effective width of the nanochannel is small compared with the persistence length of …