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Full-Text Articles in Engineering
Identification Of The [Fefe]-Hydrogenase Responsible For Hydrogen Generation In Thermoanaerobacterium Saccharolyticum And Demonstration Of Increased Ethanol Yield Via Hydrogenase Knockout, A. Joe Shaw, David A. Hogsett, Lee R. Lynd
Identification Of The [Fefe]-Hydrogenase Responsible For Hydrogen Generation In Thermoanaerobacterium Saccharolyticum And Demonstration Of Increased Ethanol Yield Via Hydrogenase Knockout, A. Joe Shaw, David A. Hogsett, Lee R. Lynd
Dartmouth Scholarship
Three putative hydrogenase enzyme systems in Thermoanaerobacterium saccharolyticum were investigated at the genetic, mRNA, enzymatic, and phenotypic levels. A four-gene operon containing two [FeFe]-hydrogenase genes, provisionally termed hfs (hydrogenase-Fe-S), was found to be the main enzymatic catalyst of hydrogen production. hfsB, perhaps the most interesting gene of the operon, contains an [FeFe]-hydrogenase and a PAS sensory domain and has several conserved homologues among clostridial saccharolytic, cellulolytic, and pathogenic bacteria. A second hydrogenase gene cluster, hyd, exhibited methyl viologen-linked hydrogenase enzymatic activity, but hyd gene knockouts did not influence the hydrogen yield of …
Quantifying Total And Sustainable Agricultural Biomass Resources In South Dakota—A Preliminary Assessment, Kurt A. Rosentrater, Dennis Todey, Russell Persyn
Quantifying Total And Sustainable Agricultural Biomass Resources In South Dakota—A Preliminary Assessment, Kurt A. Rosentrater, Dennis Todey, Russell Persyn
Kurt A. Rosentrater
Conversion of biomass is considered the next major advance in biorenewable fuels, energy, and products. Wholesale conversion to biomass utilization could result in removal of current crop residues from agricultural fields (on prime agricultural lands) or even implementation of different crops and cropping strategies (i.e., switchgrass on marginal lands). To date, the driver for biomass processing has been economics and limitations on the conversion of the lignocellulose. Over the last forty years significant investments and resultant changes in management practices in the agricultural sector have focused on soil and water conservation. One of the major efforts has focused on conservation-till …