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Articles 1 - 16 of 16
Full-Text Articles in Engineering
Development Of A Low-Cost, Open Source Miniature Rotary Cell Culture System To Simulate Microgravity Within An Irradiated Environment, Elizabeth Vargis, Jr Dennison
Development Of A Low-Cost, Open Source Miniature Rotary Cell Culture System To Simulate Microgravity Within An Irradiated Environment, Elizabeth Vargis, Jr Dennison
Browse all Datasets
A major challenge for astronauts in long-duration space travel is combatting the hazardous spaceflight environment caused by microgravity and increased levels of ionizing radiation. Microgravity damages cellular DNA by increasing the production of harmful reactive oxygen species, while ionizing radiation damages DNA by creating double-stranded DNA (dsDNA) breaks. Cellular damage due to microgravity and radiation has been investigated using ground-based models, but most models consider microgravity and ionizing radiation alone, or asynchronously. Synchronous modeling better mimics spaceflight conditions and can be used to understand the combined effects of microgravity and ionizing radiation. However, commercially available devices to model microgravity and …
A Multi-Scale Approach To Study Biochemical And Biophysical Aspects Of Resveratrol On Diesel Exhaust Particle-Human Primary Lung Cell Interaction, Wei Zhang, Qifei Li, Mingjie Tang, Han Zhang, Xiaoping Sun, Sige Zou, Judy L. Jensen, Theodore G. Liou, Anhong Zhou
A Multi-Scale Approach To Study Biochemical And Biophysical Aspects Of Resveratrol On Diesel Exhaust Particle-Human Primary Lung Cell Interaction, Wei Zhang, Qifei Li, Mingjie Tang, Han Zhang, Xiaoping Sun, Sige Zou, Judy L. Jensen, Theodore G. Liou, Anhong Zhou
Biological Engineering Faculty Publications
Diesel exhaust particles (DEPs) are major air pollutants that lead to numerous human disorders, especially pulmonary diseases, partly through the induction of oxidative stress. Resveratrol is a polyphenol that ameliorates the production of reactive oxygen species (ROS) and delays aging-related processes. Herein we studied the cytoprotective effect of resveratrol on DEP-exposed human lung cells in a factorial experimental design. This work investigates biophysical features including cellular compositions and biomechanical properties, which were measured at the single-cell level using confocal Raman microspectroscopy (RM) and atomic force microscopy (AFM), respectively. Principal component analysis (PCA), hierarchical cluster analysis (HCA) and partial least square …
Milk Oligopeptide Inhibition Of (Α)-Tocopherol Fortified Linoleic Acid Oxidation, Haina Yuan, Jinyan Gong, Kun Tang, Jinge Huang, Gongnian Xiao, Jianmin Lv
Milk Oligopeptide Inhibition Of (Α)-Tocopherol Fortified Linoleic Acid Oxidation, Haina Yuan, Jinyan Gong, Kun Tang, Jinge Huang, Gongnian Xiao, Jianmin Lv
Biological Engineering Student Research
This study investigated the effect of milk oligopeptides and (α)-tocopherol on inhibition of linoleic acid oxidation using Fe2+-vitamin C induced linoleic acid oxidation model through analysis of malondialdehyde, peroxide value, and conjugated diene and triene in the model. The alteration of milk oligopeptides maximal absorption wavelength, fluorescent feature, and secondary structure were further investigated to elucidate the interactions between milk oligopeptide and (α)-tocopherol that altered the inhibitory effect of linoleic acid oxidation. Results showed that Pro-Tyr-Tyr-Ala-Lys (PYYAK) and Ile-Pro-Ile-Gln-Tyr (IPIQY) with (α)-tocopherol significantly inhibited the oxidation of linoleic acid and reduced the formation of malondialdehyde by 38% and …
Monitoring Silane Sol-Gel Kinetics With In-Situ Optical Turbidity Scanning And Dynamic Light Scattering, Abul Bashar Mohammad Giasuddin, David W. Britt
Monitoring Silane Sol-Gel Kinetics With In-Situ Optical Turbidity Scanning And Dynamic Light Scattering, Abul Bashar Mohammad Giasuddin, David W. Britt
Biological Engineering Faculty Publications
Organosilanes (e.g., R’-SiOR3) provide hydrophobic functionality in thin-film coatings, porous gels, and particles. Compared with tetraalkoxysilanes (SiOR4), organosilanes exhibit distinct reaction kinetics and assembly mechanisms arising from steric and electronic properties of the R’ group on the silicon atom. Here, the hydrolysis and condensation pathways of n-propyltrimethoxy silane (nPM) and a tri-fluorinated analog of nPM, 3,3,3-trifluoropropyl trimethoxy silane (3F), were investigated under aqueous conditions at pH 1.7, 2.0, 3.0, and 4.0. Prior to hydrolysis, 3F and nPM are insoluble in water and form a lens at the bottom (3F) or top (nPM) of the solutions. This …
Workshop On Convergence In Biological Engineering, Keith Roper
Workshop On Convergence In Biological Engineering, Keith Roper
Funded Research Records
No abstract provided.
Application Of Micro-Scale 3d Printing In Pharmaceutics, Andrew Kjar, Yu Huang
Application Of Micro-Scale 3d Printing In Pharmaceutics, Andrew Kjar, Yu Huang
Biological Engineering Faculty Publications
3D printing, as one of the most rapidly-evolving fabrication technologies, has released a cascade of innovation in the last two decades. In the pharmaceutical field, the integration of 3D printing technology has offered unique advantages, especially at the micro-scale. When printed at a micro-scale, materials and devices can provide nuanced solutions to controlled release, minimally invasive delivery, high-precision targeting, biomimetic models for drug discovery and development, and future opportunities for personalized medicine. This review aims to cover the recent advances in this area. First, the 3D printing techniques are introduced with respect to the technical parameters and features that are …
Discovery And Engineering Of An Endophytic Pseudomonas Strain From Taxus Chinensis For Efficient Production Of Zeaxanthin Diglucoside, Ozkan Fidan, Jixun Zhan
Discovery And Engineering Of An Endophytic Pseudomonas Strain From Taxus Chinensis For Efficient Production Of Zeaxanthin Diglucoside, Ozkan Fidan, Jixun Zhan
Biological Engineering Faculty Publications
Background
Endophytic microorganisms are a rich source of bioactive natural products. They are considered as promising biofertilizers and biocontrol agents due to their growth-promoting interactions with the host plants and their bioactive secondary metabolites that can help manage plant pathogens. Identification of new endophytes may lead to the discovery of novel molecules or provide new strains for production of valuable compounds.
Results
In this study, we isolated an endophytic bacterium from the leaves of Taxus chinensis, which was identified as Pseudomonas sp. 102515 based on the 16S rRNA gene sequence and physiological characteristics. Analysis of its secondary metabolites revealed …
Modified Substrate Specificity Of A Methyltransferase Domain By Protein Insertion Into An Adenylation Domain Of The Bassianolide Synthetase, Fuchao Xu, Russell Butler, Kyle M. May, Megi Rexhepaj, Dayu Yu, Jiachen Zi, Yi Chen, Yonghong Liang, Jia Zeng, Joan Hevel, Jixun Zhan
Modified Substrate Specificity Of A Methyltransferase Domain By Protein Insertion Into An Adenylation Domain Of The Bassianolide Synthetase, Fuchao Xu, Russell Butler, Kyle M. May, Megi Rexhepaj, Dayu Yu, Jiachen Zi, Yi Chen, Yonghong Liang, Jia Zeng, Joan Hevel, Jixun Zhan
Chemistry and Biochemistry Faculty Presentations
Background: Creating designer molecules using a combination of select domains from polyketide synthases and/or nonribosomal peptide synthetases (NRPS) continues to be a synthetic goal. However, an incomplete understanding of how protein-protein interactions and dynamics affect each of the domain functions stands as a major obstacle in the field. Of particular interest is understanding the basis for a class of methyltransferase domains (MT) that are found embedded within the adenylation domain (A) of fungal NRPS systems instead of in an end-to-end architecture.
Results: The MT domain from bassianolide synthetase (BSLS) was removed and the truncated enzyme BSLS-ΔMT was recombinantly expressed. The …
Microwave Assisted Sol-Gel Synthesis Of Silica-Spider Silk Composites, Abul Bashar Mohammad Giasuddin, David W. Britt
Microwave Assisted Sol-Gel Synthesis Of Silica-Spider Silk Composites, Abul Bashar Mohammad Giasuddin, David W. Britt
Biological Engineering Faculty Publications
This study introduces a simple and environmentally friendly method to synthesize silica-protein nanocomposite materials using microwave energy to solubilize hydrophobic protein in an aqueous solution of pre-hydrolyzed organo- or fluoro-silane. Sol-gel functionality can be enhanced through biomacromolecule incorporation to tune mechanical properties, surface energy, and biocompatibility. Here, synthetic spider silk protein and organo- and fluoro-silane precursors were dissolved and mixed in weakly acidic aqueous solution using microwave technology. Scanning electron microscopy (SEM) and Atomic force microscopy (AFM) images revealed the formation of spherical nanoparticles with sizes ranging from 100 to 500 nm depending, in part, on silane fluoro- or organo-side …
Unravel The Cellular Biophysical Dynamics Of Spatial Constraint-Induced Membrane Blebbing And 3d Migration Using A Microfluidic Platform And Data-Driven Mathematical Modeling, Yu Huang
Funded Research Records
No abstract provided.
Use Of Surface-Enhanced Raman Scattering (Sers) Probes To Detect Fatty Acid Receptor Activity In A Microfluidic Device, Han Zhang, Wei Zhang, Lifu Xiao, Yan Liu, Timothy A. Gilbertson, Anhong Zhou
Use Of Surface-Enhanced Raman Scattering (Sers) Probes To Detect Fatty Acid Receptor Activity In A Microfluidic Device, Han Zhang, Wei Zhang, Lifu Xiao, Yan Liu, Timothy A. Gilbertson, Anhong Zhou
Biological Engineering Faculty Publications
In this study, 4-mercaptobenzoic acid (MBA)-Au nanorods conjugated with a GPR120 antibody were developed as a highly sensitive surface-enhanced Raman spectroscopy (SERS) probe, and were applied to detect the interaction of fatty acids (FA) and their cognate receptor, GPR120, on the surface of human embryonic kidney cells (HEK293-GPRR120) cultured in a polydimethylsiloxane (PDMS) microfluidic device. Importantly, the two dominant characteristic SERS peaks of the Raman reporter molecule MBA, 1078 cm−1 and 1581 cm−1, do not overlap with the main Raman peaks from the PDMS substrate when the appropriate spectral scanning range is selected, which effectively avoided the …
Improved Production Of Antifungal Angucycline Sch47554 By Manipulating Three Regulatory Genes In Streptomyces Sp. Scc-2136, Ozkan Fidan, Riming Yan, Du Zhu, Jixun Zhan
Improved Production Of Antifungal Angucycline Sch47554 By Manipulating Three Regulatory Genes In Streptomyces Sp. Scc-2136, Ozkan Fidan, Riming Yan, Du Zhu, Jixun Zhan
Biological Engineering Faculty Publications
Sch47554 and Sch47555 are two angucyclines with antifungal activities against various yeasts and dermatophytes from Streptomyces sp. SCC‐2136. The schgene cluster contains several putative regulatory genes. Both schA4 and schA21were predicted as the TetR family transcriptional regulators, whereas schA16shared significant similarity to the AraC family transcriptional regulators. Although Sch47554 is the major product of Streptomyces sp. SCC‐2136, its titer is only 6.72 mg/L. This work aimed to increase the production of this promising antifungal compound by investigating and manipulating the regulatory genes in the Sch47554 biosynthetic pathway. Disruption of schA4and schA16 led to a significant increase …
Develop A 3d Neurological Disease Model Of Human Cortical Glutamatergic Neurons Using Micropillar-Based Scaffolds, Cheng Chen, Xin Dong, Kai-Heng Fang, Fang Yuan, Yao Hu, Min Xu, Yu Huang, Xixiang Zhang, Danjun Fang, Yan Liu
Develop A 3d Neurological Disease Model Of Human Cortical Glutamatergic Neurons Using Micropillar-Based Scaffolds, Cheng Chen, Xin Dong, Kai-Heng Fang, Fang Yuan, Yao Hu, Min Xu, Yu Huang, Xixiang Zhang, Danjun Fang, Yan Liu
Biological Engineering Faculty Publications
Establishing an effective three-dimensional (3D) in vitro culture system to better model human neurological diseases is desirable, since the human brain is a 3D structure. Here, we demonstrated the development of a polydimethylsiloxane (PDMS) pillar-based 3D scaffold that mimicked the 3D microenvironment of the brain. We utilized this scaffold for the growth of human cortical glutamatergic neurons that were differentiated from human pluripotent stem cells. In comparison with the 2D culture, we demonstrated that the developed 3D culture promoted the maturation of human cortical glutamatergic neurons by showing significantly more MAP2 and less Ki67 expression. Based on this 3D culture …
Identification Of New Glutamate Decarboxylases From Streptomyces For Efficient Production Of Γ-Aminobutyric Acid In Engineered Escherichia Coli, Haina Yuan, Hongbo Wang, Ozkan Fidan, Yong Qin, Gongnian Xiao, Jixun Zhan
Identification Of New Glutamate Decarboxylases From Streptomyces For Efficient Production Of Γ-Aminobutyric Acid In Engineered Escherichia Coli, Haina Yuan, Hongbo Wang, Ozkan Fidan, Yong Qin, Gongnian Xiao, Jixun Zhan
Biological Engineering Faculty Publications
Background
Gamma (γ)-Aminobutyric acid (GABA) as a bioactive compound is used extensively in functional foods, pharmaceuticals and agro-industry. It can be biosynthesized via decarboxylation of monosodium glutamate (MSG) or L-glutamic acid (L-Glu) by glutamate decarboxylase (GAD; EC4.1.1.15). GADs have been identified from a variety of microbial sources, such as Escherichia coli and lactic acid bacteria. However, no GADs from Streptomyces have been characterized. The present study is aimed to identify new GADs from Streptomyces strains and establish an efficient bioproduction platform for GABA in E. coli using these enzymes.
Results
By sequencing and analyzing the genomes of three Streptomycesstrains, …
Improving Wastewater Treatment Using Algal Biofilms And Bioenergy, Nathan Guymon
Improving Wastewater Treatment Using Algal Biofilms And Bioenergy, Nathan Guymon
Research on Capitol Hill
This project represents a collaboration between Utah State University, Central Valley Water Reclamation Facility (CVWRF), and WesTech Engineering, Inc. The goal of the project is to help water treatment plants meet state nutrient standards and reduce operating costs. This is being accomplished in three major sections:
1. Wastewater treatment – microalgae is grown to remove nitrogen and phosphorus from water.
2. Biogas generation – the microalgae is harvested and processed to produce methane gas used to generate power.
3. Fertilizer production – nutrients are also collected from the wastewater and turned into high-value fertilizer.
By creating systems that remove nutrients …
Muscle Atrophy Marker Expression Differs Between Rotary Cell Culture System And Animal Studies, Charles P. Harding, Elizabeth Vargis
Muscle Atrophy Marker Expression Differs Between Rotary Cell Culture System And Animal Studies, Charles P. Harding, Elizabeth Vargis
Biological Engineering Faculty Publications
Muscular atrophy, defined as the loss of muscle tissue, is a serious issue for immobilized patients on Earth and for humans during spaceflight, where microgravity prevents normal muscle loading. In vitro modeling is an important step in understanding atrophy mechanisms and testing countermeasures before animal trials. The most ideal environment for modeling must be empirically determined to best mimic known responses in vivo. To simulate microgravity conditions, murine C2C12 myoblasts were cultured in a rotary cell culture system (RCCS). Alginate encapsulation was compared against polystyrene microcarrier beads as a substrate for culturing these adherent muscle cells. Changes after culture …