Engineering Commons^™

Analysis Of Tissue Engineering Strategies For Bone-Related Applications, Timothy Buirkle

Theses

Over the past few decades, tissue engineering approaches have pushed their way to the forefront of biotechnological research and development, serving a wide variety of applications. This research utilizes quantitative methods to analyze the outcome of two bone tissue engineering studies. In an in vivo study, the use of Negative Pressure Wound Therapy (NPWT) in combination with stem cell-loaded scaffolds was investigated for the repair of large bone defects. Quantitative histological analysis was performed by determining both the percentage of cells proliferating (BrdU), as well as cells expressing bone-specific markers of alkaline phosphatase (A L P) in the defect. Total …