Engineering Commons^™

A Rapid Co-Culture Stamping Device For Studying Intercellular Communication, Amin Hassanzadeh-Barforoushi, Jonathan Shemesh, Nona Farbehi, Mohsen Asadnia, Guan H. Yeoh, Richard P. Harvey, Robert E. Nordon, Majid E. Warkiani

Research outputs 2014 to 2021

Regulation of tissue development and repair depends on communication between neighbouring cells. Recent advances in cell micro-contact printing and microfluidics have facilitated the in-vitro study of homotypic and heterotypic cell-cell interaction. Nonetheless, these techniques are still complicated to perform and as a result, are seldom used by biologists. We report here development of a temporarily sealed microfluidic stamping device which utilizes a novel valve design for patterning two adherent cell lines with well-defined interlacing configurations to study cell-cell interactions. We demonstrate post-stamping cell viability of > 95%, the stamping of multiple adherent cell types, and the ability to control the seeded …