Engineering Commons^™

The Feasibility Of Imaging Myocardial Ischemic/Reperfusion Injury Using 99mTc-Labeled Duramycin In A Porcine Model, Lei Wang, Feng Wang, Wei Fang, Steven E. Johnson, Said H. Audi, Michael Zimmer, Thomas A. Holly, Daniel C. Lee, Bao Zhu, Haibo Zhu, Ming Zhao

Biomedical Engineering Faculty Research and Publications

When pathologically externalized, phosphatidylethanolamine (PE) is a potential surrogate marker for detecting tissue injuries. ^99mTc-labeled duramycin is a peptide-based imaging agent that binds PE with high affinity and specificity. The goal of the current study was to investigate the clearance kinetics of ^99mTc-labeled duramycin in a large animal model (normal pigs) and to assess its uptake in the heart using a pig model of myocardial ischemia–reperfusion injury.

Methods

The clearance and distribution of intravenously injected ^99mTc-duramycin were characterized in sham-operated animals (n = 5). In a closed chest model of myocardial ischemia, coronary occlusion was induced …

In Vivo Detection Of Hyperoxia-Induced Pulmonary Endothelial Cell Death Using 99mTc-Duramycin, Said H. Audi, Elizabeth R. Jacobs, Ming Zhao, David L. Roerig, Steven T. Haworth, Anne V. Clough

Biomedical Engineering Faculty Research and Publications

Introduction

^99mTc-duramycin, DU, is a SPECT biomarker of tissue injury identifying cell death. The objective of this study is to investigate the potential of DU imaging to quantify capillary endothelial cell death in rat lung injury resulting from hyperoxia exposure as a model of acute lung injury.

Methods

Rats were exposed to room air (normoxic) or > 98% O₂ for 48 or 60 hours. DU was injected i.v. in anesthetized rats, scintigraphy images were acquired at steady-state, and lung DU uptake was quantified from the images. Post-mortem, the lungs were removed for histological studies. Sequential lung sections were immunostained …