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A Novel Technique To Characterize The Surface Hydrophobicity Of Proteins Using Inverse Liquid Chromatography, Dilip Sethi, Sarah Hedberg, Daryl Williams
A Novel Technique To Characterize The Surface Hydrophobicity Of Proteins Using Inverse Liquid Chromatography, Dilip Sethi, Sarah Hedberg, Daryl Williams
Biological and Pharmaceutical Complex Fluids III: Protein Self-Assembly, Rheology and Interfacial Properties
This work presents a novel technique to characterize the surface hydrophobicity and other surface properties of proteins. The surface properties of industrial and therapeutic proteins are key to understanding their behavior in-situ, in the laboratory and in processes. Protein surface hydrophobicity is a marker for three dimensional structure, stability and function. Inverse Liquid Chromatography of Proteins (ILCP) with small molecule hydrophobic probes can be used to obtain direct measurements of the surface hydrophobicity of column resin-bound proteins using changes in probe retention behavior. The dimensionless hydrophobicity factor (Hf) for Lysozyme and BSA was obtained with changing pH …