Open Access. Powered by Scholars. Published by Universities.®
- Keyword
Articles 1 - 3 of 3
Full-Text Articles in Engineering
The Effects Of Ultrashort Pulsed Electric Fields On The Fluorescent Molecule Bodipy Fl C5 Ceramide, Rachael Sara Shevin
The Effects Of Ultrashort Pulsed Electric Fields On The Fluorescent Molecule Bodipy Fl C5 Ceramide, Rachael Sara Shevin
Electrical & Computer Engineering Theses & Dissertations
Nanosecond pulsed electric fields are able to manipulate internal cell organelles by taking advantage of the small but finite time required for charge to establish across a capacitive barrier. Pulsed electric fields also affect macromolecular structures such as enzymes and proteins. Application of a single nanosecond pulsed electric field decreases the fluorescence of a commercially available biological dye Bodipy FL C5 Ceramide, used to observe the Golgi apparatus of living cells. The effect is seen when the fluorophore is exposed to an electric field both in vitro and when incorporated into the Golgi of a living Jurkat cell. Observation …
Temporal Calcium Response Of Jurkat Cells To Nanosecond Pulsed Electric Fields, Shaka Shakar Scarlett
Temporal Calcium Response Of Jurkat Cells To Nanosecond Pulsed Electric Fields, Shaka Shakar Scarlett
Electrical & Computer Engineering Theses & Dissertations
Nanosecond pulsed electric fields (nsPEF) of megavolt per meter field strengths can interact with subcellular structures [1] and consequently trigger a variety of biological events. The exposure can induce apoptosis, as well as an array of differential "sub-lethal effects." One of the most sensitive and immediate responses is the increase in intracellular calcium concentration in pulsed cells [2], [3][4].We investigated the response of Jurkat cells (a human leukemic cell line) to nsPEFs of 60 ns duration and field strengths from 25 tol00 kV/cm. The development of the induced calcium response in individual cells was studied by spinning-disc confocal microscopy with …
Diverse Effects Of Nanosecond Pulsed Electric Fields On Mammalian Cells, Jody Anne White
Diverse Effects Of Nanosecond Pulsed Electric Fields On Mammalian Cells, Jody Anne White
Theses and Dissertations in Biomedical Sciences
The continuing effort to manipulate cell-signaling pathways for therapeutic benefit has lead to the exploration of electric field effects on cells. Current electric field applications include electroporation of the plasma membrane for introduction of drugs, genes, or other macromolecules into cells. Modeling of how these pulsed electric fields affect cells depicts the cell as an excitable circuit. In this model, the electric fields, administered in short pulses to a cell, charge the plasma and internal membranes, which act as dielectric layers, and between these the cytoplasm acts as a conductive medium. The pulse lengths of this treatment are traditionally in …