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Full-Text Articles in Engineering
Novel Platforms For Large-Scale Adherent Culture Of Mammalian Cells, Ashkan Yekrangsafakar
Novel Platforms For Large-Scale Adherent Culture Of Mammalian Cells, Ashkan Yekrangsafakar
LSU Doctoral Dissertations
With recent advances in biotechnology, there is a strong and urgent need for robust platforms to culture mammalian cells on a large scale to produce biopharmaceuticals. To this end, various bioreactors have been developed over the past decades, but their capacity and efficiency are often limited by insufficient mass transfer rate and excessive shear stress. In this work, multiple novel bioreactors for the large-scale adherent culture of anchorage-dependent cells were developed.
Hollow MicroCarriers (HMC) was developed as an alternative solution for the microcarrier-based culture system in a stirred-tank bioreactor. In the conventional microcarrier technique, cells are exposed to the harmful …
Design And Fabrication Of A Low-Cost, Portable, Battery-Operated Surface Enhanced Raman Scattering (Sers) Optical Device, Blessing Adewumi
Design And Fabrication Of A Low-Cost, Portable, Battery-Operated Surface Enhanced Raman Scattering (Sers) Optical Device, Blessing Adewumi
LSU Doctoral Dissertations
Raman Spectroscopy is a time-honored, non-invasive method for analyzing and identifying the molecular composition of materials. However, unenhanced Raman Spectroscopy has extremely low sensitivity which limits its sensing capability. SERS brings rough nano-metallic surfaces in contact with the material molecules to enormously enhance the Raman signals.
The sensitivity of SERS can be exploited in probe applications where the spectrometer needs to be brought near the specimen. For example, a long optical fiber coupled to a SERS device can be used to characterize and identify easy-to-reach cancerous tissues in organisms. Unfortunately, background signals in a long fiber can easily mask any …