Engineering Commons^™

Development Of Polymer-Based In-Plane Nanopore For Dna Sequencing, Zheng Jia

LSU Doctoral Dissertations

Mechanically robust solid-state nanopores have the potential to be the next generation DNA sensing platforms. However, mass production and limited base-calling accuracy are the hurdles for solid-state nanopore based DNA sensing. In order to solve these problems, a polymer dual-nanopore device fabricated via high throughput nanoimprint lithography (NIL) was proposed to sequence DNA by time-of-flight (ToF) measurement. As a proof of concept, this study presents mononucleotides discrimination via ToF measurement using polymer in-plane dual-nanopore device.

First, fabrication of polymer in-plane nanopore with controllable dimensions was studied in consideration of experimental conditions and materials selection. Then, surface charge density effect on …

Computational Aerodynamics And Anatomical Characterization Of Laryngotracheal Stenosis In Children, William Poynot

LSU Master's Theses

Laryngotracheal stenosis (LTS) is a health condition in which an obstruction in the upper trachea can cause breathing difficulties and increased incidence of infection, among other symptoms. Occurring most commonly due to intubation in infants, LTS often requires corrective surgery. Currently, clinical methods of assessing the blockage region are simplistic and subjective, and it is challenging to determine the most effective surgical strategy for any given patient. In the present work, a comprehensive methodology is proposed for characterizing the stenosis region both in terms of its anatomical parameters and its corresponding aerodynamic properties. The combination of computational fluid dynamics (CFD) …

Experimental, Analytical, And Numerical Evaluation Of The Mechanical Properties Of The Brain Tissue, Aref Samadidooki

LSU Doctoral Dissertations

A true understanding of the mechanisms behind most of the brain diseases is still out of reach. For several years, the interest of scientists has been focused on the genetic and biological causes, however, recent studies unraveled the importance of the biomechanics of the brain growth, folding, impact resistance, and deformation on its pathological conditions. While, a wide range of different methods have been used for characterization of the mechanical properties of the brain at the tissue level, the obtained results from different studies are extremely scattered and sometimes in contrast to one another. Since the brain tissue is extremely …

Microfluidic Technology And Application In Urinal Analysis, Jiwen Xiang

LSU Doctoral Dissertations

Microfluidic technology offers numerous advantages in minimizing and integrating the traditional assays. However, the lack of efficient control components of the microfluidic systems has been hindering the widely commercialization of the technology. The research work in this dissertation focused on the development of effective control components for microfluidic applications.

A linear peristaltic pump was firstly designed, fabricated, and tested for conventional microfluidics by synchronously compressing the microfluidic channel with a miniature cam-follower system in Chapter 2. The miniature cam-follower system and microfluidic chip was prototyped using three-dimensional (3D) printing technology and soft lithography technology. Results from experimental test showed that …