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Full-Text Articles in Engineering
Characterization And Optimization Of A Chromatographic Process Based On Ethylenediamine-N,N,N′,N′-Tetra(Methylphosphonic) Acid-Modified Zirconia Particles, Sabyasachi Sarkar, Peter W. Carr, Clayton V. Mcneff, Anuradha Subramanian
Characterization And Optimization Of A Chromatographic Process Based On Ethylenediamine-N,N,N′,N′-Tetra(Methylphosphonic) Acid-Modified Zirconia Particles, Sabyasachi Sarkar, Peter W. Carr, Clayton V. Mcneff, Anuradha Subramanian
Papers in Biotechnology
The primary objective of work was to characterize, optimize and model a chromatographic process based on ethylenediamine-N,N,N′,N′-tetra(methylphosphonic) acid (EDTPA)-modified zirconia particles. Zirconia particles were produced by spray-drying colloidal zirconia. Zirconia spheres produced were further classified, calcined and modified with EDTPA to yield a solid-phase support for use in bio-chromatography (r_PEZ). Specifically, the ability of r_PEZ to selectively bind and enrich IgG, IgA, and IgM from biological fluids was evaluated and demonstrated. To better understand the force of interaction between the IgG and the r_PEZ, the equilibrium disassociation constant (Kd) was determined by static binding isotherms, as a function of temperature …
Transgenic Non-Human Mammals Expressing Human Coagulation Factor Viii And Von Willebrand Factor, Henryk Lubon, William N. Drohan, William H. Velander
Transgenic Non-Human Mammals Expressing Human Coagulation Factor Viii And Von Willebrand Factor, Henryk Lubon, William N. Drohan, William H. Velander
Papers in Biotechnology
A non-human transgenic mammalian animal, as described above, contains an exogenous double stranded DNA sequence stably integrated into the genome of the animal, which comprises cis-acting regulatory units operably linked to a DNA sequence encoding human Factor VIII protein and a signal peptide, where the cis-acting regulatory units are active in mammary gland cells and the signal peptide is active in directing newly expressed Factor VIII into the milk of the animal. The promoter may be a milk protein promoter such as for whey acidic protein, casein, lactalbumin, or beta-lactoglobulin promoter. The transgenic mammals are preferably farm animals, for example, …
Pichia Pastoris Fermentation With Mixed-Feeds Of Glycerol And Methanol: Growth Kinetics And Production Improvement, Wenhui Zhang, Karen J. Hywood Potter, Bradley A. Plantz, Vicki L. Schlegel, Leonard A. Smith, Michael M. Meagher
Pichia Pastoris Fermentation With Mixed-Feeds Of Glycerol And Methanol: Growth Kinetics And Production Improvement, Wenhui Zhang, Karen J. Hywood Potter, Bradley A. Plantz, Vicki L. Schlegel, Leonard A. Smith, Michael M. Meagher
Papers in Biochemical Engineering
Fed-batch fermentation of a methanol utilization plus (Mut+) Pichia pastoris strain typically has a growth phase followed by a production phase (induction phase). In the growth phase glycerol is usually used as carbon for cell growth while in the production phase methanol serves as both inducer and carbon source for recombinant protein expression. Some researchers employed a mixed glycerol-methanol feeding strategy during the induction phase to improve production, but growth kinetics on glycerol and methanol and the interaction between them were not reported. The objective of this paper is to optimize the mixed feeding strategy based on growth kinetic studies …