Health and Physical Education Commons^™

Is The Skeletal Muscle Hypoxic Response To Exercise Different Between Endurance Trained And Untrained Individuals?, Roberto C. Nava, Zachary Fennel, Zachary Mckenna, Quint Berkemeier, Flávio De Castro Magalhães, Michael Deyhle, Fabiano Amorim, Christine Mermier

Health, Exercise, and Sports Sciences ETDs

It was previously hypothesized that endurance exercise training blunts the hypoxia inducible factor 1 (HIF-1) response to acute exercise. We investigated whether training status influences the skeletal muscle HIF-1 response to acute exercise. 7 endurance athletes and 8 untrained controls performed a bout of cycling exercise. Skeletal muscle protein levels of HIF-1α and its inhibitors, prolyl hydroxylase 2 (PHD2), factor inhibiting HIF (FIH) and von Hippel-Lindau (VHL) were measured pre, post and 3h post-exercise. HIF-1 target gene expression was assessed via rtPCR and RNA-sequencing. Baseline protein levels of FIH and PHD2 were greater in the trained group. The exercise-induced increase …