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Design Of A Robust Undergraduate Biochemistry Laboratory Course Based On A Modified And Expanded Bovine Serum Albumin Purification Scheme, Odutayo O. Odunuge, Nicholas Cheatwood, John Mullins, Michele Harris
Design Of A Robust Undergraduate Biochemistry Laboratory Course Based On A Modified And Expanded Bovine Serum Albumin Purification Scheme, Odutayo O. Odunuge, Nicholas Cheatwood, John Mullins, Michele Harris
Symposium on Arts and Research
The ASBMB curriculum for an undergraduate degree recommends a set of skills that can be acquired only through laboratory courses and research experience. Based on a previously reported purification scheme for BSA (Odunuga and Shazhko, 2013), we designed a robust, reproducible, cost-effective, safe and enquiry-based undergraduate biochemistry laboratory course that encompasses many of the skill-sets recommend-ed in the ASBMB curriculum. Our work not only modi-fied certain steps in the scheme, it also included addi-tional steps to enhance student learning and skill ac-quisition. Salt precipitation, ion exchange and size ex-clusion chromatography were employed by students to purify BSA from cow plasma. …