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Bacterial Community Profiling Of The Eastern Oyster (Crassostrea Virginica): Comparison Of Culture-Dependent And Culture-Independent Outcomes, Kenneth J. La Valley, Steve Jones, Marta Gomez-Chiarri, Joseph Dealteris, Michael A. Rice

Michael A Rice

Tissue-associated bacterial community profiles generated using a nested polymerase chain reaction–denaturing gradient gel electrophoresis (DGGE) approach and culture-dependent and culture-independent isolation techniques were compared. Oyster samples were collected from 2 harvest areas along the coast of Maine, in the United States. Profiles from both isolation strategies were evaluated using Sorensen’s index of similarity and cluster analysis of gel banding patterns. Cultureindependent profiles were further evaluated using the Shannon diversity index. In general, the culture-dependent strategy resulted in a greater number of bands within a profile. BacterialDGGEprofiles were found to be highly similar within an isolation strategy, with a higher degree …