Open Access. Powered by Scholars. Published by Universities.®
- Keyword
-
- Humans (3)
- Adolescent (2)
- Antigens, Viral (2)
- CD8-Positive T-Lymphocytes (2)
- Child (2)
-
- Dengue (2)
- Dengue Virus (2)
- Epitopes, T-Lymphocyte (2)
- Female (2)
- Interferon-gamma (2)
- Thailand (2)
- Viral Envelope Proteins (2)
- *Immunologic Memory (1)
- Adoptive Transfer (1)
- Aging (1)
- Animals (1)
- Antibodies, Viral (1)
- Antigens, CD27 (1)
- Asymptomatic Infections (1)
- B-Lymphocytes (1)
- Chemokine CCL4 (1)
- Child, Preschool (1)
- Cohort Studies (1)
- Cross Reactions (1)
- Enzyme-Linked Immunosorbent Assay (1)
- Enzyme-Linked Immunospot Assay (1)
- Hemagglutination Inhibition Tests (1)
- Human Experimentation (1)
- Immunity, Cellular (1)
- Immunodominant Epitopes (1)
- File Type
Articles 1 - 6 of 6
Full-Text Articles in Arts and Humanities
Development Of Antigen-Specific Memory Cd8+ T Cells Following Live-Attenuated Chimeric West Nile Virus Vaccination, Heidi Smith, Thomas Monath, Pamela Pazoles, Alan Rothman, Diane Casey, Masanori Terajima, Francis Ennis, Farshad Guirakhoo, Sharone Green
Development Of Antigen-Specific Memory Cd8+ T Cells Following Live-Attenuated Chimeric West Nile Virus Vaccination, Heidi Smith, Thomas Monath, Pamela Pazoles, Alan Rothman, Diane Casey, Masanori Terajima, Francis Ennis, Farshad Guirakhoo, Sharone Green
Sharone Green
ChimeriVax-WN02 is a novel live-attenuated West Nile virus (WNV) vaccine containing modified WNV premembrane (prM) and envelope (E) sequences inserted into the yellow fever 17D vaccine genome. We investigated the induction and evolution of CD8(+) T cell responses to a WNV envelope epitope, which is a dominant target in naturally infected HLA-A*02-positive individuals. WNV epitope-specific CD8(+) T cells were detected by HLA tetramer staining in 22 of 23 donors tested, with peak frequencies occurring between days 14 and 28. WNV epitope-specific T cells evolved from an effector phenotype to a long-lived memory phenotype. In the majority of donors, CD8(+) T …
Determinants Of Inapparent And Symptomatic Dengue Infection In A Prospective Study Of Primary School Children In Kamphaeng Phet, Thailand, Timothy P. Endy, Kathryn B. Anderson, Ananda Nisalak, In-Kyu Yoon, Sharone Green, Alan L. Rothman, Stephen J. Thomas, Richard G. Jarman, Daniel H. Libraty, Robert V. Gibbons
Determinants Of Inapparent And Symptomatic Dengue Infection In A Prospective Study Of Primary School Children In Kamphaeng Phet, Thailand, Timothy P. Endy, Kathryn B. Anderson, Ananda Nisalak, In-Kyu Yoon, Sharone Green, Alan L. Rothman, Stephen J. Thomas, Richard G. Jarman, Daniel H. Libraty, Robert V. Gibbons
Sharone Green
BACKGROUND: Dengue viruses are a major cause of morbidity in tropical and subtropical regions of the world. Inapparent dengue is an important component of the overall burden of dengue infection. It provides a source of infection for mosquito transmission during the course of an epidemic, yet by definition is undetected by health care providers. Previous studies of inapparent or subclinical infection have reported varying ratios of symptomatic to inapparent dengue infection. METHODOLOGY/PRINCIPAL FINDINGS: In a prospective study of school children in Northern Thailand, we describe the spatial and temporal variation of the symptomatic to inapparent (S:I) dengue illness ratio. Our …
B-Cell Responses During Primary And Secondary Dengue Virus Infections In Humans, Anuja Mathew, Kim West, Siripen Kalayanarooj, Robert Gibbons, Anon Srikiatkhachorn, Sharone Green, Daniel Libraty, Smita Jaiswal, Alan Rothman
B-Cell Responses During Primary And Secondary Dengue Virus Infections In Humans, Anuja Mathew, Kim West, Siripen Kalayanarooj, Robert Gibbons, Anon Srikiatkhachorn, Sharone Green, Daniel Libraty, Smita Jaiswal, Alan Rothman
Sharone Green
Low-avidity serotype-cross-reactive antibodies are hypothesized to play a key role in triggering severe disease in patients with secondary dengue virus (DENV) infection. However, there is little systematic information about the frequency, avidity, and cross-reactivity of DENV-specific B cells in individuals experiencing primary instead of secondary infection. We compared DENV-specific B-cell responses in a cohort of Thai children with primary or secondary DENV infection. B cells specific for DENV precursor membrane protein, envelope (E) protein, and nonstructural protein 1 were detectable in immune peripheral blood mononuclear cells with the highest frequencies of DENV E-specific B cells detected in patients experiencing primary …
Long Term Recall Of Memory Cd8 T Cells In Mice To First And Third Generation Smallpox Vaccines, Sharone Green, Francis Ennis, Anuja Mathew
Long Term Recall Of Memory Cd8 T Cells In Mice To First And Third Generation Smallpox Vaccines, Sharone Green, Francis Ennis, Anuja Mathew
Sharone Green
Since long-term immunity is a critical component of any effective vaccine, we compared over a 15-month period, the strength, durability and specificity of immunity of an attenuated smallpox vaccine Modified Vaccinia Ankara (MVA) to the New York City Board of Health (NYCBH) vaccine. The frequencies of CD8(+) T cells to an immunodominant CD8 T cell epitope B8R(20-27) remained remarkably stable in mice given either MVA or NYCBH. Both groups were also protected from a lethal intranasal challenge with Western Reserve strain of vaccinia virus (VACV-WR). Cytokine responses to virus-specific peptides were detectable with significant boosting upon challenge. Expression of most …
T-Cell Responses To Dengue Virus In Humans, Ichiro Kurane, Takaji Matsutani, Ryuji Suzuki, Tomohiko Takasaki, Siripen Kalayanarooj, Sharone Green, Alan L. Rothman, Francis A. Ennis
T-Cell Responses To Dengue Virus In Humans, Ichiro Kurane, Takaji Matsutani, Ryuji Suzuki, Tomohiko Takasaki, Siripen Kalayanarooj, Sharone Green, Alan L. Rothman, Francis A. Ennis
Sharone Green
Dengue virus (DENV) is a leading cause of morbidity and mortality in most tropical and subtropical areas of the world. Dengue virus infection induces specific CD4+CD8- and CD8+CD4- T cells in humans. In primary infection, T-cell responses to DENV are serotype cross-reactive, but the highest response is to the serotype that caused the infection. The epitopes recognized by DENV-specific T cells are located in most of the structural and non-structural proteins, but NS3 is the protein that is most dominantly recognized. In patients with dengue hemorrhagic fever (DHF) caused by secondary DENV infection, T cells are highly activated in vivo. …
Cross-Reactivity And Expansion Of Dengue-Specific T Cells During Acute Primary And Secondary Infections In Humans, Heather L. Friberg-Robertson, Hema Sundara Bashyam, Tomoko Toyosaki-Maeda, James A. Potts, Thomas C. Greenough, Siripen Kalayanarooj, Robert V. Gibbons, Ananda Nisalak, Anon Srikiatkhachorn, Sharone Green, Henry A. F. Stephens, Alan L. Rothman, Anuja Mathew
Cross-Reactivity And Expansion Of Dengue-Specific T Cells During Acute Primary And Secondary Infections In Humans, Heather L. Friberg-Robertson, Hema Sundara Bashyam, Tomoko Toyosaki-Maeda, James A. Potts, Thomas C. Greenough, Siripen Kalayanarooj, Robert V. Gibbons, Ananda Nisalak, Anon Srikiatkhachorn, Sharone Green, Henry A. F. Stephens, Alan L. Rothman, Anuja Mathew
Sharone Green
Serotype-cross-reactive memory T cells responding to secondary dengue virus (DENV) infection are thought to contribute to disease. However, epitope-specific T cell responses have not been thoroughly compared between subjects with primary versus secondary DENV infection. We studied CD8(+) T cells specific for the HLA-A*1101-restricted NS3(133) epitope in a cohort of A11(+) DENV-infected patients throughout acute illness and convalescence. We compared the expansion, serotype-cross-reactivity, and activation of these cells in PBMC from patients experiencing primary or secondary infection and mild or severe disease by flow cytometry. Our results show expansion and activation of DENV-specific CD8(+) T cells during acute infection, which …