Digital Commons Network^™

Resolving Intra-Tumoral Heterogeneity Of Breast Cancer Using Single-Cell Multi-Omics, Yun Yan

Dissertations and Theses (Open Access)

Breast cancer is a highly heterogeneous disease characterized by diverse genetic, transcriptional, and epigenetic landscapes. Understanding how intra-tumoral heterogeneity (ITH) influences therapeutic response and drives tumor evolution remains a central challenge in cancer biology. Here, we leverage single-cell multi-omics technologies to dissect the cell states and gene expression programs underlying breast cancer heterogeneity, with a focus on triple-negative breast cancer (TNBC) and estrogen receptor-positive (ER+) breast cancer.

In the first part, we applied single-cell RNA sequencing and spatial transcriptomics to profile over 400,000 cells from 101 treatment-naïve TNBC patients. We identified 4 archetypes at the patient level and 13 metaprograms …

Facilitating The Clinical Translation Of Flash Radiotherapy Through Dosimetry Development And Beam Parameter Optimization, Kevin Liu

Dissertations and Theses (Open Access)

Advisory Professor: Emil Schüler, PhD

Radiation therapy (RT) is a crucial component of curative cancer therapy, with a majority of cancer patients in the United States receiving RT as part of treatment. The goal of RT is to maximize the therapeutic index in curing disease while minimizing any associated normal-tissue complications¹. Recently, ultra-high dose-rate (UHDR) RT (mean dose rates ≥40 Gy/s for a total duration of ≤200 ms) has been reported to selectively spare normal tissues and organs while maintaining an isoeffective tumoricidal effect compared to conventional (CONV) dose rate RT in a variety of in vivo preclinical …

Structural Functional Investigation Of Hap40, Amanda M. Solbach

Dissertations and Theses (Open Access)

STRUCTURAL FUNCTIONAL INVESTIGATION OF HAP40

Amanda Marie Solbach

Dissertation Advisor: Sheng Zhang, Ph.D.

Huntington’s disease (HD) is a devastating neurodegenerative disorder caused by an expanded CAG trinucleotide repeat in the Huntingtin (HTT) gene, resulting in a mutant huntingtin (mHTT) protein with an elongated polyglutamine tract. While the genetic basis of HD is well established, the mechanisms underlying mHTT toxicity and its impact on cellular function remain incompletely understood. Recent discoveries implicate Huntingtin-associated protein 40 (HAP40) as a critical regulator of HTT stability and intracellular function. However, the physiological and pathological significance of this interaction has not been fully explored.

This …

Modeling Renal Anomalies Associated With Li-Fraumeni Patients: A Novel Role For P53 In Kidney Development, Alejandra Davilavaladez

Dissertations and Theses (Open Access)

Li-Fraumeni syndrome (LFS) is a heritable disorder caused by germline mutations in the TP53 gene that result in an increased risk of cancer. The TP53 tumor suppressor gene regulates cell division and prevents the accumulation of cells that may become cancerous. LFS patients are susceptible to various types of cancers, including osteosarcoma, soft tissue sarcoma, acute leukemia, and adrenal cortical tumors, as well as breast and brain cancer. Additionally, these patients have an increased risk of developing kidney, stomach, colon, pancreas, esophagus, lung, and gonadal germ cell cancers. Prior studies indicate that p53 is involved in kidney development. However, there …

Long-Chain Fatty Acids Promote Candida Albicans Gut Colonization By Restructuring The Fungal Cell Surface, Musfirat Shubaita

Dissertations and Theses (Open Access)

Long-chain fatty acids promote Candida albicans gut colonization by restructuring the fungal cell surface

Musfirat Shubaita, BS

Advisory Professor: J. Christian Perez, Ph.D.

The yeast Candida albicans is a facultative anaerobe residing in the human digestive tract. Although fungal genetic determinants of mammalian host colonization have been identified, little is known about signals and molecules in the intestinal environment that influence C. albicans proliferation. Previously unpublished data from the Perez lab showed that oleic acid, one of the most abundant long-chain fatty acids in nature, promotes C. albicans colonization of the murine intestine. The goal of this research is to …

The Effects Of A Tier 2 Intervention On The Behavior Of Elementary School Students Participating In Second Step: Enhancing A Schoolwide Social-Emotional Learning Curriculum, Charlotte Annalee Kennington

Theses and Dissertations

After reviewing the current research literature on the effects of social-emotional learning (SEL) on the behavior of elementary-age children, it was clear that there was limited research data on the effects of Tier 2 interventions designed to augment the effects of schoolwide SEL programs. Specifically, to what extent could a brief, group-delivered intervention improve the academic engagement, problem behavior, and socially appropriate comments of fifth-grade students receiving schoolwide SEL through Second Step in a traditional public school? Three fifth-grade students were recruited to participate in this study who all engaged in frequent problem behavior during Second Step lessons and throughout …

Executive Committee - Agenda, 08/01/2025, Academic Senate

Academic Senate Agendas

No abstract provided.

The Experiences Of Elite, Female Club Volleyball Players – An Interpretative Phenomenological Analysis, Chelsea Brehm

Dissertations

The purpose of this qualitative study was to investigate the lived experiences of female athletes participating in national-level club volleyball in a Midwestern U.S. state. Amid increasing sport privatization and a growing emphasis on early sport specialization, female athletes in club volleyball are navigating complex pressures tied to elite competition, college recruitment, and psychosocial well-being. Using Interpretative Phenomenological Analysis (IPA), this study sought to uncover how athletes experience and make meaning of their participation in this highly demanding sport structure.

Data collection included a demographic survey distributed to national-level athletes (n = 66) and in-depth, semistructured interviews with five female …

Drug Delivery, Disulfide Crosslinked Hydrogel Coating For Polydimethylsiloxane (Pdms) Implant Materials, Paul A. Contos, Paul Anthony Contos

Master's Theses

Though polydimethylsiloxane (PDMS) is often used in medical devices due to its desirable mechanical properties and biocompatibility, its surface is often prone to bacterial adhesion. This is especially a problem for catheters, where bacteria can adhere and form biofilms that can in turn result in Urinary Tract Infections (UTI). In order to deter the presence of UTI causing bacteria on PDMS catheters, methods were developed to formulate a disulfide crosslinked hydrogel coating and create a methodology to allow adhesion to a PDMS substrate. The hydrogel coating discussed in this paper will be synthesized by chemically crosslinking stimuli-responsive vesicles, that carry …

Internal Funding Newsletter, Academic Year 2024-2025, Office Of Research And Creative Activity, University Of Nebraska At Omaha

Internal Funding Newsletters

The University of Nebraska at Omaha is committed to educating people of the world and the Office of Research and Creative Activity is proud to contribute to UNO?s exceptional education, groundbreaking research, and the life-long success of students and alumni. UNO innovates for the public good through pragmatic and impactful research and discovery. This year's programs provided over $750,000 in awards for students and faculty. These projects included recording a new album of music for a saxophone, flute, and piano trio, understanding college campus bicycle theft, studying student perceptions of the difficulty of organic chemistry concepts, evaluating a therapy program …

Revolutionizing Digital Privacy Education For Older Adults: Enhanced Interventions And Ai-Assisted Learning Strategies, Heba Aly

All Dissertations

As older adults increasingly engage with digital platforms, they face unique privacy risks stemming from limited digital literacy, reduced trust in AI technologies, and constrained access—especially in rural or underserved communities. While digital tools offer benefits like social connection and information access, current privacy education efforts often neglect the needs of older adults. This dissertation addresses this gap by developing, testing, and refining digital privacy education interventions tailored for older adults, with a focus on trust, personalization, and AI-assisted learning.

Study 1 evaluates multiple instructional modalities across age groups, revealing older adults prefer structured videos and interactive tutorials, while younger …

A Study On The Propagation And Exploitation Of Structured Light In Underwater Turbulence, Jaxon P. Wiley

All Dissertations

The development and optimization of optical systems will play a pivotal role in the continued exploration and exploitation of the world’s underwater environments. These systems offer advantages in many sectors, and includes applications in areas such as high-speed communication, advanced sensing and imaging, and environmental characterization and monitoring. Underwater environments offer a plethora of challenges, however, and mitigating these obstacles remains an arduous task. In this work, the inherent advantages of structured light are leveraged to optimize optical system performance through non-ideal underwater conditions. Additionally, fundamental relationships between the generation of specified structured modes and their interactions with complex environments …

Multiple View Neural Regression Of A Facial Shape Model, Xiang Li

All Dissertations

Creating re-topologized 3D facial meshes is a critical step in high-quality facial animation pipelines, yet it remains a labor-intensive and time-consuming task. Traditional approaches typically rely on multiview stereo reconstruction and specialized photometric environments to acquire accurate geometric and reflectance data under controlled conditions. This dissertation presents work toward more efficient capture of production-ready meshes including (1) developmental aspects of VarIS, a custom-designed light sphere capable of capturing high-resolution stereo geometry and reflectance maps—including diffuse, specular, and normal components under programmable illumination; (2) a study of the effects of camera parameters on automatic 2D and 3D landmarking methods, (3) methods …

Designing Multilingual (Maker) Spaces: Exploring The Intersections Of Play, Posthumanism, And Pre-Kindergarten, Victoria G. Pennington

All Dissertations

In this dissertation, I explore the posthuman play literacies of multilingual makerspaces with four-year-olds, emphasizing the need for such a study while situating my arguments within the existing literature and outlining my methodological approach. Chapter One critiques the public 4-year-old kindergarten systems, arguing institutionalized cultural and linguistic ideologies restrict multilingual learners’ (MLs) opportunities for meaning-making. I draw on the raciolinguistic perspective (Flores & Rosa, 2015) and its intersection with a posthumanist paradigm (Braidotti, 2013), framing my research questions as lines of inquiry to guide the study. Key posthumanist literacies concepts are defined, highlighting the entanglement of literacies, discourses, materials, and …

Engaging Minds: Assessing The Impact Of Arts-Integration On Science Achievement And Engagement Using Planned Experimentation, Fonda G. Dupre

All Dissertations

Elementary science education is essential for fostering inquiry, critical thinking, and problem-solving, yet it is often reduced or deprioritized due to the pressures of high-stakes testing in reading and mathematics. Despite federal calls for a well-rounded education under the Every Student Succeeds Act (2015), instructional time for science remains limited in many schools, particularly in under-resourced and high-poverty contexts. This reduction of science instruction time exacerbates achievement gaps and limits student engagement and interest in STEM fields.

This planned experiment investigates whether Arts-Integrated science instruction can increase student engagement and improve academic achievement among fourth-grade students at Foothills Academy of …

Influence Of Experience And Risk With Natural Hazards On Geoscience Interest And Career Expectations, Shannon Conner

All Dissertations

Understanding student interest in geoscience is important, as it has the potential to inform departmental decisions such as the selection of classroom examples and marketing strategies to attract new geoscience majors. Recruitment to geoscience is critical to address future social and environmental challenges. Our work may also provide insight into how to approach persistent recruitment problems within departments, such as student enrollment and the lack of diversity within the geosciences. This work involves developing a geoscience interest scale, integrating geospatial and educational data sets related to hazard risk and interest, and an exploring career motivations as related to natural hazard …

Exploring The Effects Of Remote Work On Leadership Pipelines: With A Focus On Professional Women And Minorities, Mark S. Andrekovich

All Dissertations

I analyzed data from five U.S.-based companies to understand how remote work models affect leadership advancement. Using Role Congruity, Status Characteristics, and Intersectionality theories, I analyzed five hypotheses about the impact of remote work on leadership pipelines. Two of the five hypotheses were supported, revealing bias patterns rather than issues related to remote work. For instance, white employees had greater access to remote work opportunities than non-white employees, who had fewer promotions despite similar performance scores. Contrary to expectations, I found that remote work does not inherently affect promotional advancements. Via a post hoc analysis, I found that hybrid professionals …

Transforming Childhood Adversity Into Executive Excellence: Impact On Firm Performance, Kimberly Byars

All Dissertations

Many successful business leaders have overcome difficult childhoods, yet little research has examined how early hardships might actually contribute to leadership effectiveness. This study investigated whether CEOs who experienced childhood adversity can transform these difficult experiences into strengths that improve their companies' performance.

The research surveyed 165 business owners across two groups: general business owners and automotive dealership owners. Participants answered questions about their childhood experiences, their ability to grow from trauma, and various psychological traits like grit, mental toughness, resilience, and growth mindset. Company performance data was also collected.

The findings challenge conventional wisdom that difficult childhoods necessarily harm …

Organizational Metamorphosis: When Artificial Intelligence Transforms Companies Into New Economic Life Forms, Allan M. Glass

All Dissertations

Traditional theories of organizational change fall short in explaining how artificial intelligence (AI) transforms organizations. This grounded theory study, based on interviews with 34 organizational leaders, identifies a novel phenomenon: organizational metamorphosis - the irreversible transformation of organizations into fundamentally new entities through deep integration with AI as a cognitive partner.

The metamorphosis process unfolds in three phases. Individual Awakening occurs when leaders experience powerful interactions with AI, prompting a rapid shift from skepticism to advocacy. Once a critical mass of leaders undergo this shift, it initiates Organizational Restructuring, characterized by reimagined work design, boundary dissolution, and altered temporal rhythms. …

Evaluating The Impact Of Medicaid Expansion On Non-Small Cell Lung Cancer Care And Health Disparities, Marvin Idoteyin Okon

All Dissertations

Persistent disparities in non-small cell lung cancer (NSCLC) outcomes reflect broader structural inequities shaped by race, place, and socioeconomic conditions. This dissertation examined trends in NSCLC outcomes over the past two decades and evaluated the impact of Medicaid Expansion (ME) under the Affordable Care Act on improving outcomes and reducing disparities, especially in marginalized communities.

Using nationally representative datasets, three interrelated studies were conducted. The first assessed trends in NSCLC incidence, early-stage diagnosis, and 3-year survival from 2002-2021, stratified by metropolitan status, deprivation (Social Deprivation Index), and segregation (Index of Concentration at the Extremes). While incidence declined and early-stage diagnosis …

Melts To Solutions: Structure, Dynamics, And Response Of Polyelectrolytes, Shalika Meedin

All Dissertations

The current research elucidates the changes in the structure and motion of polyelectrolytes as they are perturbed by solvents and shear flows. Polyelectrolytes are macromolecules whose backbone consists of long, predominantly hydrocarbon chains, substituted by ionizable groups, which tend to dissociate into ions in polar solvents. Their properties result from a delicate balance of elastic energy that arises from the polymeric backbone and electrostatic forces, which stem from the ionizable groups. Though polyelectrolytes have an immense potential to propel numerous technologies, from clean energy to drug delivery systems, understanding the delicate balance between the properties of the polymer backbone and …

Bringing To Life Our Mission And Vision: The Role Of Professional Development In Instructional Technology, Katherine I. Britton

All Dissertations

Despite improved access to digital tools and increased demand to prepare students for the 21st-century workforce, K-12 educators still have difficulty balancing technology, pedagogy, and content in the classroom. In the rural school district of Pleasant County, there is variation in the utilization of instructional technology to engage students as content creators, collaborators, critical thinkers, and communicators. This problem was substantiated as evidenced by the study of the Effective Learning Observation Tool, which identified the domain of engaging, learning-centered instruction as an area of opportunity for the district, with the lowest score in digital learning (Cognia, 2023). In this study, …

Accurate Temporal Integration Schemes For Nonlinear Adsorption Problems, Evan D. Butterworth

All Dissertations

We consider a nonlinear transport problem to model the chromatography process of high-capacity multimodal membranes. Robust and efficient algorithms that simulate these bioseparation processes are critical to developing therapeutics for various chronic illnesses and infectious diseases. However, much of the current methodology focuses on stabilization and linearization techniques, often implementing low-order time-discretizations and linearized adsorption, resulting in inefficiencies and inaccuracies in the numerical solution. Utilizing Rothe's method, we develop various time-discretization schemes coupled with the finite element method to solve the fully implicit problems. Stability and solvability results are presented for several methods. Through multiple high-level software implementations paired with …

Towards Securing Ai Systems: Investigating Threats In Multimodal Autonomous Driving & Rag Systems, Saket Sanjeev Chaturvedi

All Dissertations

Artificial Intelligence (AI) systems have become central to high-stakes applications such as autonomous driving and language-based decision support. As their deployment accelerates, ensuring the security and trustworthiness of these systems becomes paramount. Among the most stealthy and potent threats are backdoor attacks, where models behave as expected under normal conditions but exhibit malicious behavior when triggered by specific inputs, either digital or physical.

This thesis investigates novel backdoor and adversarial vulnerabilities across two emerging classes of AI architectures: (1) multimodal 3D object detection systems that fuse LiDAR and camera data, and (2) Retrieval-Augmented Generation (RAG) systems that pair large language …

Constructing Code-Based Zero-Knowledge Proofs Leveraging Generic Errors And Bounded Vectors, Freeman Slaughter

All Dissertations

Quantum computing is developing at an expeditious rate, and once fully scalable quantum computers become realized, classical cryptographic systems face obsolescence. This approaching peril has prompted a paradigm shift away from pre-quantum cryptography and towards post-quantum primitives, such as those that arise from the field of coding theory. Among these, zero-knowledge proofs have emerged as a dynamic tool instrumental in constructing quantum-resilient digital signature schemes.

We being by introducing HammR, a pre-quantum zero-knowledge proof protocol designed to verify Hamming weight and entry constraints of error vectors, and comprehensively establish its security. Subsequently, we extend HammR to the multi-party computation setting, …

Emotional Safety In Pediatrics: The Impact Of Child Life Services On Healthcare Professionals’ Perceptions Of Their Own, Patients’, And Families’ Outpatient Experiences, Jenni Davis

All Dissertations

Medical visits, regardless of setting, are stressful for children and their families. Care that focuses on the emotional safety of children and families can help reduce this stress. However, not all staff feel prepared to provide emotionally safe care. Child life specialists (CLSs) are specifically trained to support emotional needs during healthcare encounters. Their presence may help staff stress as well, not just stress for children and families.

This study used three surveys to understand stress among healthcare staff working in pediatric outpatient clinics and surgery settings, and how support from a CLS affects staff, children, and families. All staff …

Exploring The Socio-Cultural Dynamics Of Nocturnal Urban Landscapes: A Case Study, Jie Kong

All Dissertations

This dissertation explores nighttime social leisure activities in public parks within urban working- and middle-class communities. As cities confront climate change, shifting work schedules, and post-pandemic lifestyles, neighborhood parks have gained renewed importance after dark, especially in areas where households have limited private space. These non-commercial nighttime uses are often overlooked in planning, design, and built environment studies, yet they play a vital role in fostering cultural identity, social connection, and community well-being.

Focusing on Williamsburg and Bushwick in Brooklyn, New York, this research investigates how public parks are used at night, by whom, and for what purposes. New York …

Are They Ready? Impacting Kindergarten Readiness Through The Science Of Reading, Traci T. Boyles

All Dissertations

The purpose of this research study was to investigate how Science of Reading (SOR) professional development for preschool teachers, implemented through collaborative teaching and assessment cycles, influences the kindergarten readiness of preschool students.

Students from impoverished families start kindergarten at a considerable disadvantage regarding literacy. Literacy-focused curricula and professional development for teachers strongly affect reading outcomes. Multi-Tiered Systems of Support (MTSS) provides a systemic framework for data-based problem solving and decision-making. The MTSS framework aligns literacy interventions with tiered supports to ensure all students receive the assistance they need to achieve reading proficiency.

The South Carolina Child Early Reading Development …

Online Multiobjective Optimization, Kristen Joyce

All Dissertations

Online optimization (OO) is an iterative process of decision making under uncertainty. At every step, a decision is made before the outcome of this decision is known. For the online optimization model, the objective function is unknown at the time the decision is being made. It is very likely that the taken decision is not optimal, so the decision maker incurs a loss, called regret, in every iteration. The goal of the online optimization algorithm is to compute a decision at every step so that the overall regret cost is minimized. In particular, the average regret produced by an ideal …

Nature Playscape: Investigating Children’S Play Behavior And Environmental Affordances In A School Playground, Sara E. Billie

All Dissertations

Existing research demonstrates that the physical environment can either hinder or enhance children’s play behavior. Diverse outdoor settings are especially effective in fostering motivation to play, contributing to children’s physical, social, and cognitive development. In response to the growing belief that outdoor play is both a necessity and a right for young children, particularly amidst concerns about nature deficit disorder and the rise of urban living among children, this study investigates the concept of nature playscapes, or naturalized playgrounds, as a vehicle for reconnecting children with the natural world. While contemporary playground design often struggles to balance structure with creativity, …