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Articles 30601 - 30630 of 1823802
Full-Text Articles in Entire DC Network
Implementing A Problem-Solving Framework In Physics Tutorials For Upper-Division Electricity And Magnetism Students, James C. Hecht
Implementing A Problem-Solving Framework In Physics Tutorials For Upper-Division Electricity And Magnetism Students, James C. Hecht
Undergraduate Honors Theses
We study the effects of implementing a problem-solving framework in tutorials intended for use in upper-division electricity and magnetism courses. These tutorials were integrated into an electricity and magnetism course in Fall 2024, and were compared alongside a set of tutorials on the same topics which did not include the problem-solving framework. We performed a mixed-methods analysis on exam scores, interviews, and survey responses from students who were in the class. Students seem to prefer the tutorials that implement the problem-solving framework, and these tutorials seem to assist students with conceptual understanding somewhat; however, the qualitative data on conceptual understanding …
Evaluation Of Nurses' Leadership Self-Efficacy In Belize, Ruth Rosalee Reina Jones
Evaluation Of Nurses' Leadership Self-Efficacy In Belize, Ruth Rosalee Reina Jones
Walden Dissertations and Doctoral Studies
Nursing leadership in Belize is lacking but crucial to the well-being of patients, families, and the healthcare team. The purpose of this non-experimental, descriptive, comparative study was to a) determine the difference in leadership self-efficacy for current nurse leaders with three years or less of leadership experience compared to current nurse leaders with more than three years of leadership experience in Belize, and b) examine the differences in leadership self-efficacy between nurses in a leadership position and nurses not in a leadership position in Belize. The theories supporting the study were Burns’ transformational leadership theory and leadership self-efficacy, based on …
The Lumberjack, March 25, 2026
The Lumberjack, March 25, 2026
Cal Poly Humboldt's Student Newspaper
The student newspaper of Humboldt State University.
Fs Agenda 2026-03-25, Northern Illinois University
Fs Agenda 2026-03-25, Northern Illinois University
Faculty Senate Archives
No abstract provided.
Employability Skills Development In Secondary Education, Mark D. Sims
Employability Skills Development In Secondary Education, Mark D. Sims
Morehead State Theses and Dissertations
A capstone submitted in partial fulfillment of the requirements for the degree of Doctor of Education in the Ernst and Sara Lane Volgenau College of Education at Morehead State University by Mark D. Sims on March 25, 2026.
A Scoping Review Of Gender-Based Violence Interventions Conducted In Afghanistan, Wahiba Abu-Ras, Basil H. Aboul-Enein, Patricia J. Kelly, Dilshod Achilov
A Scoping Review Of Gender-Based Violence Interventions Conducted In Afghanistan, Wahiba Abu-Ras, Basil H. Aboul-Enein, Patricia J. Kelly, Dilshod Achilov
College of Nursing Faculty Papers & Presentations
BACKGROUND: Gender-based violence (GBV) remains one of the most pervasive human rights and public health challenges worldwide, with disproportionate impacts on women and girls in conflict-affected and fragile states. Afghanistan represents one of the most restrictive environments for women's rights, where structural, cultural, and political barriers severely limit the implementation and evaluation of GBV-prevention programs. This scoping review aims to identify and critically appraise GBV prevention and response interventions implemented in Afghanistan and assess their contribution to advancing gender and health equity.
METHODS: Following PRISMA-ScR guidelines and the PICOS framework, nine databases were systematically searched for studies published between 2010 …
Electrochemical Sensors And Processes In Molten Salts: Chlorine Production, Electroanalytical Chemistry, And Thin-Layer Electrodes, Tyler Williams
Electrochemical Sensors And Processes In Molten Salts: Chlorine Production, Electroanalytical Chemistry, And Thin-Layer Electrodes, Tyler Williams
Theses and Dissertations
Molten salt electrochemistry has historically played a substantial role in the metallurgical industry, and this trend is only accelerating in areas of green steel, direct ore processing, and rare-earth processing. In addition, the recent surge of interest in molten salt nuclear reactors has provided an additional application for molten salt electrochemical processes, including the reprocessing of used nuclear fuels and the development of electrochemical sensors for nuclear applications. In this work, the development of a chloride volatility separation process for used nuclear fuel is advanced; electroanalytical methods are improved for identifying and quantifying unknown analytes with and without the presence …
Efficacy Of Individualised Homoeopathic Medicines As An Adjunct To Conventional Iron And Folic-Acid Supplementation In Iron Deficiency Anaemia Among Reproductive-Aged Women: Single-Blind, Randomised, Placebo-Controlled Trial, Avidipta Hazra, Tanmay Sarkar, Debarsi Das, Rakesh Ghosh, Chandrani Ghosh
Efficacy Of Individualised Homoeopathic Medicines As An Adjunct To Conventional Iron And Folic-Acid Supplementation In Iron Deficiency Anaemia Among Reproductive-Aged Women: Single-Blind, Randomised, Placebo-Controlled Trial, Avidipta Hazra, Tanmay Sarkar, Debarsi Das, Rakesh Ghosh, Chandrani Ghosh
Indian Journal of Research in Homoeopathy
Background: Iron deficiency anaemia (IDA) is a major public health concern affecting women of reproductive age globally. If left untreated, it can cause various health issues including fatigue, shortness of breath, abnormal heart rhythms, chest pain, and complications during pregnancy. Objectives: To evaluate the efficacy of individualised homoeopathic medicines (IHMs) with iron and folic acid supplement (IFS) in treatment of iron deficiency among reproductive-aged women, in comparison with placebos with IFS. Methods: Single-blind, randomised, placebo-controlled clinical trial was conducted over total six months at the outpatient-department, of which participants received interventions and outcome assessments for three months. Reproductive women (15–49 …
Investigation Of Molecular Footprints In Ultra-High Diluted Homoeopathic Solutions Of Arnica Montana And Rhus Toxicodendron Through Combined Infrared And Impedance Spectroscopy, Sharbadeb Kundu, Usha Rani Dash, Chirasree Roychaudhuri, Aindrila Roy, Sanatan Chattopadhyay, Rathin Chakravarty, Debadatta Nayak, Subhash Kaushik, Amit Roy Chowdhury, Ananya Barui, Subhadip Chakraborty
Investigation Of Molecular Footprints In Ultra-High Diluted Homoeopathic Solutions Of Arnica Montana And Rhus Toxicodendron Through Combined Infrared And Impedance Spectroscopy, Sharbadeb Kundu, Usha Rani Dash, Chirasree Roychaudhuri, Aindrila Roy, Sanatan Chattopadhyay, Rathin Chakravarty, Debadatta Nayak, Subhash Kaushik, Amit Roy Chowdhury, Ananya Barui, Subhadip Chakraborty
Indian Journal of Research in Homoeopathy
Background: The study of ultra-high diluted (UHD) solutions is a compelling and controversial domain in modern science. Despite numerous investigations and hypotheses, there is a lack of rigorous, reproducible empirical evidence supporting the existence of molecular footprints beyond the Avogadro's limit. Objective: This study conducted a detailed investigation of the molecular and electrical properties, focusing on hydrogen bonding and impedance to understand the fundamental nature of UHD solutions. Materials and methods: Combined impedance and infrared spectroscopic investigations were performed to probe the structural behaviour of relevant water networks at the molecular level of UHD alcoholic extract of Arnica montana (AM) …
Non-Invasive Treatment For Indolent Chronic Venous Ulcer Using Individualised Homoeopathy: An Evidence-Based Case Series, Vinitha Edavattath Ramanan, Geethu Kp
Non-Invasive Treatment For Indolent Chronic Venous Ulcer Using Individualised Homoeopathy: An Evidence-Based Case Series, Vinitha Edavattath Ramanan, Geethu Kp
Indian Journal of Research in Homoeopathy
Background: Venous ulcers account for 60–70% of all leg ulcers. They are irregular ulcers with sloping edges mainly seen above the medial malleolus associated with oedema, lipodermatosclerosis and hemosiderosis. Conventional medicine mentions chronic venous ulcers as a surgical disease. It usually results in further co-morbidities and reduces the patient's quality of life. Homoeopathic literature describes many medicines for the treatment of varicose ulcers. This case series demonstrates the role of homoeopathic treatment in the complete healing of venous ulcers non-invasively. Case Summary: This is a case series of five patients suffering from chronic venous ulcers resistant to conventional external medications …
Panic Disorder Treated With Individualised Homoeopathic Medicines: A Case Series, Hima Bindu Ponnam, Gr Kiranmayee Rompicherla
Panic Disorder Treated With Individualised Homoeopathic Medicines: A Case Series, Hima Bindu Ponnam, Gr Kiranmayee Rompicherla
Indian Journal of Research in Homoeopathy
Introduction: Panic disorder is one category of generalised anxiety disorder that is commonly reported in a majority of the outpatient departments of primary care hospitals, especially in the COVID-19 post-pandemic era. Although its prevalence is lower compared to other anxiety disorders, panic disorder remains a serious concern, as it often leads to cardiorespiratory emergencies; therefore, prompt recognition and timely management are crucial. Since the first-line pharmacological interventions of modern medicine are expensive and associated with certain undesirable side effects, the homoeopathic system of medicine appears to be a safer and more economical alternative in such cases. Keeping this in view, …
Homoeopathy During One Hundred Years Of Lmhi (1925–2025) – Part 2: Historical Perspectives* (Continued From Part 1, Published In The Last Issue)1, Josef M. Schmidt
Homoeopathy During One Hundred Years Of Lmhi (1925–2025) – Part 2: Historical Perspectives* (Continued From Part 1, Published In The Last Issue)1, Josef M. Schmidt
Indian Journal of Research in Homoeopathy
Taking Hahnemann in his entirety, as a paradigmatic example of a true homoeopath, as a sound basis for a proper understanding of Homoeopathy, the ``scientific-critical'' school of homoeopaths seems to miss one constitutive part of Homoeopathy: its spiritual embedment into a greater frame of thinking and feeling. In fact, the greatest successes of Homoeopathy, in terms of popularisation, institutionalisation and implementation into social and cultural practice, were achieved in countries and periods open towards spiritual dimensions. This was the case in North America at the end of the 19th century, when James Tyler Kent propagated a Hahnemannian Homoeopathy inspired …
Fundamental Research Principles In Homoeopathy: Bridging Empirical And Clinical Evidence, Himanshu Shekhar, Parth Aphale, Shashank Dokania
Fundamental Research Principles In Homoeopathy: Bridging Empirical And Clinical Evidence, Himanshu Shekhar, Parth Aphale, Shashank Dokania
Indian Journal of Research in Homoeopathy
Letter to Editor
Supplemental Data For Gulf Coast Walleye Edna Survey, Kevin W. Jones
Supplemental Data For Gulf Coast Walleye Edna Survey, Kevin W. Jones
Research Data
This file contains the full eDNA collection and amplification dataset for:
Jones, K.W. (2026). Development and application of an eDNA protocol for the detection of Gulf Coast Walleye (Sander vitreus) in Mississippi [Thesis submitted for publication]. Department of Wildlife, Fisheries, and Aquaculture, Mississippi State University.
Multi-Modal Tensor Fusion For Alzheimer’S Disease Recognition, Mason Li, Tiffany Le, Jiajing Huang, Yuxin Wen
Multi-Modal Tensor Fusion For Alzheimer’S Disease Recognition, Mason Li, Tiffany Le, Jiajing Huang, Yuxin Wen
Engineering Faculty Articles and Research
Accurate and early diagnosis of Alzheimer’s disease (AD) is critical for effective intervention, disease monitoring, and patient care. Traditional diagnostic approaches rely on a single modality, such as clinical assessments, neuroimaging, or genetic markers, which may fail to capture the complex, multifaceted nature of AD. Multimodal learning has therefore been explored to integrate complementary information across data sources. However, conventional fusion strategies, including early feature concatenation and late decision-level fusion, often model modalities independently and fail to capture high-order cross-modal interactions. To address these limitations, we propose a multimodal tensor fusion network (MTFN) that integrates heterogeneous data sources, including visual …
A Grounded Theory Study Of Parent Social Support For Peer-Victimized Children, Madeline Glawe
A Grounded Theory Study Of Parent Social Support For Peer-Victimized Children, Madeline Glawe
Dissertations (1934 -)
Peer-victimization/bullying (PVB) is a common adverse childhood experience associated with significant psychosocial consequences including mental health concerns (e.g., internalizing problems, anxiety, depression), behavioral problems, and poorer academic outcomes. Although existing research has established that parental social support serves as a protective factor for victimized children, little research has explored how parents provide support over time, how their internal experiences shape their provision of support, and how parents make decisions about the support they are delivering. This dissertation used Constructivist Grounded Theory to explore the process through which parents provided social support to children experiencing PVB. Semi-structured interviews were conducted with …
"What Is Called Rest, Is Not": An Iqbalian Response To Goodson, Shifa Amina Noor
"What Is Called Rest, Is Not": An Iqbalian Response To Goodson, Shifa Amina Noor
Journal of Scriptural Reasoning
No abstract provided.
The Evolution Of Self-Awareness In Conscious Organisms, Robert A. Dielenberg
The Evolution Of Self-Awareness In Conscious Organisms, Robert A. Dielenberg
Journal of Conscious Evolution
Self-awareness in conscious organisms remains an enduring puzzle for philosophers and neuroscientists. This paper proposes a tripartite evolutionary model tracing its emergence through three sequential types: the sensory self (primitive egocentric, valence-tagged awareness of sensory states), the bodily self (external objectification of the body as a distinct entity, evidenced by mirror self-recognition in select species), and the cognitive self (internal objectification of the mind enabling recursive self-transformation). Adopting a monist framework, the model treats consciousness as the biological substrate of delayed neural apperception (the “Delayed Present”), within which selves emerge as increasingly sophisticated models. It distinguishes non-human metacognition—implicit, online regulation—from …
Atrial Fibrillation In Athletes: Mechanisms, Management, And Future Directions, Ibrahim Alameh, Ghina Al Fout, Michael D. Ezekowitz, Mohammed Kamareddine
Atrial Fibrillation In Athletes: Mechanisms, Management, And Future Directions, Ibrahim Alameh, Ghina Al Fout, Michael D. Ezekowitz, Mohammed Kamareddine
Department of Medicine Faculty Papers
BACKGROUND: High-volume endurance training may shift exercise from cardioprotective to arrhythmogenic, increasing the risk of atrial fibrillation (AF) in healthy athletes.
CASE SUMMARY: A 38-year-old marathon runner with a 15-year history of high-intensity endurance training presented with episodic palpitations and reduced athletic performance. Electrocardiogram showed paroxysmal AF; echocardiography revealed mild left atrial enlargement without structural heart disease. Symptoms worsened during the recovery phase after runs, suggesting vagally mediated AF. After unsuccessful flecainide therapy, he underwent pulmonary vein isolation. Twelve months later, he remained free of recurrent AF on Holter monitoring and successfully resumed endurance training following an 8-week recovery period. …
Spatial Narrative Beyond The Proscenium: Set Design As A Tool For Wellness, Christina Griffey
Spatial Narrative Beyond The Proscenium: Set Design As A Tool For Wellness, Christina Griffey
LSU Master's Theses
Inspired by a health crisis that led to spending time in medical settings, this thesis examines how our surroundings impact our well-being. By developing a framework that explores the topics of storytelling, space, place, theatre, and expanded scenography, I propose that set design can be used as a tool for wellness. I explore several disciplines through the lens of set design and highlight the disciplines, professionals, and designers that are contributing to the creation of therapeutic environments using scenographic practices.
Qualitative Versus Quantitative Analysis Of Entheses: Using An Ordinal Scale And 3d Scanned Metrics To Understand Entheseal Differences In The Charity Hospital Cemetery (16or108), Gillian Stewart
LSU Master's Theses
Using a sample from New Orleans’ Charity Hospital Cemetery (16OR108), an analysis between ordinal scores and digital quantitative analysis (DQA) measurements of the entheses (muscle attachment sites) was performed. The skeletal elements (n = 235) examined were the humerus, radius, femur, and tibia. The entheses were 3D scanned using an Artec Space Spider scanner and processed with the Artec Studio Professional© v.2018 and Geomagic Wrap® v.2021 software. Entheses were analyzed by: left and right sides, male and female, and distribution of scores across elements. The analyses yielded significant differences between proliferative and erosive score distribution. Scores between elements exhibited variation. …
“Why Does My White Coat Feel So Tight?”: A Program Evaluation Of Well-Being Support For Physician Assistant Students, Danielle Castro
“Why Does My White Coat Feel So Tight?”: A Program Evaluation Of Well-Being Support For Physician Assistant Students, Danielle Castro
USF Tampa Graduate Theses and Dissertations
This dissertation presents a program evaluation of the Wellness Initiatives (WI) Program in the University of South Florida’s Physician Assistant (PA) Program, an accelerated 24-month graduate curriculum. The problem of practice is that PA students reported burnout, feeling overwhelmed, and difficulty navigating support within a year-round structure that did not consistently align with centralized university services. Guided by Theory of Change as the theoretical framework and Improvement Science as the conceptual framework, the evaluation examined how iterative wellness supports shaped students’ perceptions of well-being across six implementation cycles (May 2023–March 2025). A convergent parallel mixed methods design integrated survey data …
Mentoring Mentalities Made Manifest: Toward A Better Understanding Of Secondary Teacher Mentor Professional Learning, Practice, And Self-Efficacy, Maggie L. Wilson
Mentoring Mentalities Made Manifest: Toward A Better Understanding Of Secondary Teacher Mentor Professional Learning, Practice, And Self-Efficacy, Maggie L. Wilson
USF Tampa Graduate Theses and Dissertations
Teacher shortages are becoming increasingly problematic nationwide. Teacher mentoring has been found to be an effective method of curbing teacher attrition, but little attention has been addressed to ensuring that teacher mentors are receiving the most relevant professional learning experiences to bolster their self-efficacy and mentoring practice. This qualitative study, drawing on research traditions such as grounded theory and case study, seeks to illuminate the mentor self-efficacy and practice of secondary teacher mentors and to understand the role their professional learning plays in how and how well they enact their mentor roles. Through qualitative interviews, including artifact-based interviews, this study …
What Does Next-Generation Mass Spectrometry Offer For Proteomics? A Comprehensive Platform Comparison, Filipa Blasco Tavares Pereira Lopes, Daniela Schlatzer, Tara Sudhadevi, Anantha Harijith, Marzieh Ayati, Mehmet Koyutürk, Mark R. Chance
What Does Next-Generation Mass Spectrometry Offer For Proteomics? A Comprehensive Platform Comparison, Filipa Blasco Tavares Pereira Lopes, Daniela Schlatzer, Tara Sudhadevi, Anantha Harijith, Marzieh Ayati, Mehmet Koyutürk, Mark R. Chance
Computer Science Faculty Publications
Next-generation mass spectrometry platforms (Orbitrap Astral, timsTOF Ultra) are reshaping proteomics by enhancing analytical depth and sensitivity. We compared these platforms against Orbitrap Exploris 480 using neonatal mouse lung tissues from a bronchopulmonary dysplasia model (n = 12), employing four acquisition strategies: Exploris 480 DDA/DIA, Astral HR-DIA, and timsTOF Ultra DIA-PASEF. All platforms identified ∼4000 proteins in common, with 98% proteome coverage of data-dependent acquisition (DDA) identifications using data-independent (DIA) methods and 92% concordance between next-generation systems. Orbitrap Astral and timsTOF Ultra quantified >225,000 peptides and 13,000 proteins, representing ∼800% and ∼300% greater depth than Exploris 480 DDA, respectively. …
Scarcity Rewritten: Revenue Tracking As A Catalyst For Growth Mindset And Financial Control, Christian M. Pearson
Scarcity Rewritten: Revenue Tracking As A Catalyst For Growth Mindset And Financial Control, Christian M. Pearson
Undergraduate Honors Theses
While revenue tracking is a widely recognized “best practice” for businesses in developed economies, it is far from a universal practice. In Cambodia and other emerging markets, many small business owners do not track revenue or expenses, which may limit their ability to make sound financial decisions and reinforce psychological responses consistent with chronic resource scarcity. This article examines the effects of a business support intervention in which Cambodian business owners participated in an 8-week program designed to help them calculate profits by tracking revenue, variable costs, and fixed costs. Findings from a randomized field experiment show that business owners …
Quantifying Regional Variation Of Lipid Metabolism In The Brain, Martin D. Sorensen
Quantifying Regional Variation Of Lipid Metabolism In The Brain, Martin D. Sorensen
Undergraduate Honors Theses
Apolipoprotein E gene (APOE) and its associated isoforms are the most significant genetic risk factors for Alzheimer’s disease (AD). The common alleles, APOE2/3/4, exhibit a protective/neutral/risk-inducing impact on sporadic AD development respectively. Our lab’s research focuses on APOE modification of the brain proteome and lipidome, with an emphasis on regional regulation and interest in assessing how APOE4 affects regulation differently between distinct regions of the brain. This thesis presents an analysis of cryosectioned brain samples harvested from young APOE knock-in mice. Using metabolic labeling with deuterated water, we measure turnover and dietary sourcing of lipids. We observe significant differences in …
Homoeopathic Management Of Mania With Psychotic Symptoms-A Case Report, R. Bhuvaneswari, Sakthivel V
Homoeopathic Management Of Mania With Psychotic Symptoms-A Case Report, R. Bhuvaneswari, Sakthivel V
Indian Journal of Research in Homoeopathy
Introduction: Manic episode (ICD-F30.2) is typically characterised by distinct period of abnormally and persistently elevated, expansive, or irritable mood, along with abnormally and persistently increased goal-directed activity or energy, lasting at least 1 week and causing significant disruption in occupational and social functioning. The lifetime risk of experiencing manic episodes is approximately 0.8–1%. These episodes typically recur in three to four-month cycles. This case report aims to highlight significance of individualised homoeopathic treatment in managing mania with psychotic symptoms over a 41-month period without relapse, and without the use of conventional medication. Case Summary: An 18-year-old female was brought …
Holistic Approach Of Homoeopathy In Treatment Of Multimorbidity: A Case Report, Divya Verma, Nitin Kumar Saklani, Arun Kumar
Holistic Approach Of Homoeopathy In Treatment Of Multimorbidity: A Case Report, Divya Verma, Nitin Kumar Saklani, Arun Kumar
Indian Journal of Research in Homoeopathy
Introduction: Multimorbidity, a rise in all ages, is a challenge for health care systems that are focused on single-disease management. Homoeopathy has shown competence in the management of chronic diseases, whether they occur as a single ailment or as multimorbidities. Case Summary: A 25-year-old female suffering from symptoms of PCOS, like irregular menses, secondary dysmenorrhea and secondary amenorrhea, along with chronic migraine visited the OPD. She also developed renal calculi during the previous homoeopathic treatment. Her condition improved through homoeopathic medicines Pulsatilla and Lycopodium. The case is reported according to HOM-CASE guidelines. The assessment of causal attribution of homoeopathic …
First-Order Axial Perturbation Of The Reissner–Nordström Metric Against A Possible Parity-Violating Gravity Background, Abhishek Rout, Brett David Altschul
First-Order Axial Perturbation Of The Reissner–Nordström Metric Against A Possible Parity-Violating Gravity Background, Abhishek Rout, Brett David Altschul
Faculty Publications
We study axial perturbations of Reissner–Nordström black holes within the general frame-work of parity-violating modified gravity theories. We derive the governing equations for a class of frame-dragging perturbations, focusing on the symmetry structure and radial dependence of the perturbed metric component, describing its behavior across three distinct regions: near the singularity (r → 0), between the inner and outer Reissner–Nordström horizons (r− < r < r+), and in the asymptotic exterior regime (r → ∞). Using a combination of analytical and numerical methods, we analyze the solutions for varying black hole charge-to-mass ratios (Q/M) and angular momentum parameters (l). Key findings include the suppression of perturbations by the electromagnetic field for higher Q/M; the emergence of radial resonance-like behavior for specific l values; and a high degree of symmetry for solutions in the extremal limit (Q/M ∼ 1), attributed to the AdS2 × S 2 near-horizon geometry. The WKB approximation is employed to study the high-l regime, revealing quantized radial resonance modes and singular behavior in the extremal limit. Additionally, we explore the role of boundary conditions and the possibility of a Chern–Simons field Θ as the source of the parity violation, showing that consistency and the behavior of the perturbations under time reversal demand a constant field (and thus no actually observable Chern–Simons effects) at leading order. These results provide a basis for further analysis of the stability and dynamical properties of charged black holes in parity-violating theories, with potential experimental signatures in gravitational wave observations.
Modulation Of Biomolecular Aggregate Morphology And Condensate Infectivity., Josephine C Ferreon, Kyoung-Jae Choi, My Diem Quan, Phoebe S Tsoi, Cristopher C Ferreon, Ulas Coskun, Shih-Chu Jeff Liao, Allan Chris M Ferreon
Modulation Of Biomolecular Aggregate Morphology And Condensate Infectivity., Josephine C Ferreon, Kyoung-Jae Choi, My Diem Quan, Phoebe S Tsoi, Cristopher C Ferreon, Ulas Coskun, Shih-Chu Jeff Liao, Allan Chris M Ferreon
Faculty, Staff and Students Publications
Neurodegenerative diseases feature diverse pathological protein aggregates, including Lewy bodies in Alzheimer's disease (AD) and skein-like filaments in amyotrophic lateral sclerosis (ALS). The physical mechanisms underlying this morphological diversity remain unclear. Here, we demonstrate that aggregation of the prion-like domain of hnRNPA1 (A1PrD), implicated in AD and ALS, is driven by solution composition and phase transition dynamics. Utilizing 3D timelapse and fluorescence lifetime imaging microscopy, we show that solution conditions modulate phase separation, gelation, and fibrillation, resulting in distinct structures such as fibril, gel, and starburst morphologies. Homotypic and heterotypic interactions between A1PrD and RNA were observed to shift the …