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Articles 19171 - 19200 of 1817890
Full-Text Articles in Entire DC Network
Ile Idasile Aso: Creating Spaces That Promote The Creative Economy In Lagos., Ifeoluwa S. Gandonu
Ile Idasile Aso: Creating Spaces That Promote The Creative Economy In Lagos., Ifeoluwa S. Gandonu
Bachelor of Architecture Theses - 5th Year
Ilé Ìdásílẹ̀ Aṣọ directly translates to “House of Cloth Creation”. Yoruba fashion is more than attire; it is a cultural system that encodes identity, resilience, and social belonging through fabric. Every textile from the indigo-dyed adire of Osogbo to the ceremonial aso-oke woven in Abeokuta carries a story of community, heritage, and continuity. These materials serve not merely as garments but as living archives that express the Yoruba worldview through color, pattern, and form. Just as fashion weaves meaning into cloth, architecture can weave culture into space. Both disciplines involve composition, layering, and adaptation, reflecting how societies construct and express …
From Camps To Communities: A Spatial System For Refugee Recovery, Integration, And Independence, Neeya Yahaya
From Camps To Communities: A Spatial System For Refugee Recovery, Integration, And Independence, Neeya Yahaya
Bachelor of Architecture Theses - 5th Year
Global displacement has reached unprecedented levels, with over 114 million people forcibly displaced worldwide (UNHCR, 2024). Although refugee camps are intended as temporary emergency responses, the average duration of displacement has extended to approximately 17 years. During this time, displaced populations are typically housed in rural, border-adjacent settlements with limited access to infrastructure, economic systems, and social services. Designed for short-term survival, these environments often fail to support long-term recovery, resulting in prolonged dependency and continued exposure to instability.
This research defines this condition as temporary permanence: refugees remain in camps for years or decades in environments not designed to …
Piece By Peace: Reimagining Healing Spaces For Us Veterans, Gerdon Chavez
Piece By Peace: Reimagining Healing Spaces For Us Veterans, Gerdon Chavez
Bachelor of Architecture Theses - 5th Year
The study of U.S. Veterans mental illness presents a unique opportunity to explore how architecture can respond to the psychological, emotional and economic struggles through spaces that provide solutions beyond conventional institutional healthcare models. Findings suggest that veterans continue to experience complex forms of trauma, including but not limited to, post-traumatic stress disorder (PTSD) and traumatic brain injury (TBI) and existing clinical spaces are often disconnected, making the reintegration back into society harder. This study will examine how architecture can shift its focus from housing a facility to becoming part of the process by integrating housing, therapeutic spaces and community …
Building For Shared Experience:Common Ground Through Interaction, Belonging & Elective Learning At Clarkston High School, Tiana Mcbride
Building For Shared Experience:Common Ground Through Interaction, Belonging & Elective Learning At Clarkston High School, Tiana Mcbride
Bachelor of Architecture Theses - 5th Year
This thesis explores how adaptive reuse and spatial organization can transform a typical high school experience into spaces that support collective belonging and organic interaction. Located in Clarkston, Georgia, a city recognized for its diverse immigrant populations, the project expresses the transition from literal cultural representation to the formation of unified space, without assimilation.
The proposal reuses the existing structural framework of a former school building. Reorganized through a central atrium strategy with the addition of elective class learning to facilitate the natural gathering of students within the spaces they love and choose. Elective programs, commons, library spaces, and shared …
Getting To The Heart Of The Problem: Blood Pressure Management Through Medication Adherence, Mallory G. Livengood
Getting To The Heart Of The Problem: Blood Pressure Management Through Medication Adherence, Mallory G. Livengood
Evidence-Based Practice Project Reports
Clinical hypertension affects one in four adults globally, making it the most common chronic condition. Despite its widespread impact, hypertension can be prevented, as it represents the most modifiable risk factor in preventing cardiovascular disease. The primary purpose of this evidence-based project was to determine whether a one-month follow-up with a healthcare provider improved medication adherence through enhanced hypertension management. Twelve eligible participants were selected from a primary clinic located in Northwest Indiana, based on their newly initiated anti-hypertensive medications or who required a medication adjustment due to poor hypertension management. Each participant’s preferred method of communication was noted, and …
Breastfeeding Promotion For Infants With Neonatal Abstinence Syndrome (Nas), Olivia Czerwonka
Breastfeeding Promotion For Infants With Neonatal Abstinence Syndrome (Nas), Olivia Czerwonka
Evidence-Based Practice Project Reports
Neonatal abstinence syndrome (NAS) is the exposure in utero to drugs with the incidence in the United States increasing from 4 to 6.3 per 1000 births (Wei et al., 2023). The purpose of this quality improvement project was to implement a breastfeeding support intervention to increase breastfeeding frequency and reduce hospital length of stay of newborns with NAS. A tailored multicomponent guideline based upon the current Ten Steps to Successful Breastfeeding as established by WHO and UNICEF (2017) was implemented. Mothers of infants diagnosed with NAS were recruited using convenience sampling from a Northwest Indiana Hospital. Key stakeholders within the …
The Case For A Measured Approach To Second-Generation Litigation Finance Reform, Robert Weber
The Case For A Measured Approach To Second-Generation Litigation Finance Reform, Robert Weber
Buffalo Law Review
For the past decade and a half, third-party litigation finance (TPLF) has become an important part of the U.S. civil litigation system, helping litigants (especially plaintiffs) relieve litigation-related financial stresses and prosecute claims to enforce their legal rights. In response to the industry’s appearance, a first generation of TPLF regulation unfolded from roughly 2010 until 2022. This first-generation reform program was a state law phenomenon, with a dozen or so states enacting a series of tentative and piecemeal reforms. Over the past three years, and in response to intense policy demands of business groups like the Institute for Legal Reform …
Hey Siri—Can The Police Search My Period Data?, Tyonna J. Acoff
Hey Siri—Can The Police Search My Period Data?, Tyonna J. Acoff
Buffalo Law Review
No abstract provided.
“Justice” By Deception: The Immigration Regime’S Longstanding Reliance On Fraud And Coercion, Lori A. Nessel
“Justice” By Deception: The Immigration Regime’S Longstanding Reliance On Fraud And Coercion, Lori A. Nessel
Buffalo Law Review
No abstract provided.
Improved Nutritional Value Of Spinach (Spinacia Oleracea L.) Through Moderate Salinity-Induced Regulation Of Vitamin C And Folate Pathways, Laleh Ahmadi, Hanieh Mohajjel Shoja, Elham Mohajel Kazemi, Maryam Kolahi
Improved Nutritional Value Of Spinach (Spinacia Oleracea L.) Through Moderate Salinity-Induced Regulation Of Vitamin C And Folate Pathways, Laleh Ahmadi, Hanieh Mohajjel Shoja, Elham Mohajel Kazemi, Maryam Kolahi
Turkish Journal of Botany
Soil salinity is a major abiotic stress affecting over 20% of cultivated and 33% of irrigated lands worldwide, significantly limiting crop productivity and nutrient biosynthesis. Vitamin C and folate are crucial antioxidants in plant stress tolerance and human nutrition; however, their regulation under salt stress remains unclear, particularly in leafy vegetables. This study evaluated the effects of different salinity levels (0, 50, 150, 250, and 350 mM NaCl) on physiological traits, antioxidant activity, vitamin C and folate accumulation, and the expression of related biosynthetic genes in spinach (Spinacia oleracea L.). High salinity (250–350 mM) reduced growth, leaf area, and pigment …
Identification Of Qtls And Molecular Markers Associated With Gray Mold Resistance In Grape, İlknur Polat, İbrahi̇m Çeli̇k, Onur Ergönül, Asli Polat, İlker Kurbetli̇, Görkem Sülü, Ayfer Daldal, Hali̇l İbrahi̇m Uzun
Identification Of Qtls And Molecular Markers Associated With Gray Mold Resistance In Grape, İlknur Polat, İbrahi̇m Çeli̇k, Onur Ergönül, Asli Polat, İlker Kurbetli̇, Görkem Sülü, Ayfer Daldal, Hali̇l İbrahi̇m Uzun
Turkish Journal of Botany
Grape (Vitis vinifera L.) is an economically important fruit that is both consumed fresh from the vine and used in the production of raisins, wine, and vinegar. One of the main factors limiting grape production is gray mold, caused by the fungal pathogen Botrytis cinerea. The cultivation of mold-resistant grape cultivars represents the most effective strategy for disease management; however, molecular tools to aid in the breeding of B. cinerea-resistant grapes remain limited. The present study maps the quantitative trait loci (QTLs) associated with B. cinerea resistance in grape using an F1 population derived from a reciprocal cross between the …
Type-1 Ryanodine Receptor Plays An Important Role In Cardiac Hypertrophy And Heart Failure By Increasing Type-2 Ryanodine Receptor-Mediated Calcium Release, Yong-Xiao Wang, Ed Wilson Santos, Sarahann Mistretta, Yuexing Yuan, Harold A. Singer, Shey-Shing Sheu, Yun-Min Zheng
Type-1 Ryanodine Receptor Plays An Important Role In Cardiac Hypertrophy And Heart Failure By Increasing Type-2 Ryanodine Receptor-Mediated Calcium Release, Yong-Xiao Wang, Ed Wilson Santos, Sarahann Mistretta, Yuexing Yuan, Harold A. Singer, Shey-Shing Sheu, Yun-Min Zheng
Center for Translational Medicine Faculty Papers
Type-1 ryanodine receptor (RyR1) is essential for skeletal muscle contraction. This Ca2+ release channel is expressed in cardiac myocytes; however, its function remains elusive. Cardiac-specific RyR1 overexpression (OE) mice were generated under the cardiac-specific Myh6 promoter. Cardiac hypertrophy (CH), cardiac functions, and mechanistic changes in RyR1 OE and control (wildtype, WT) mice were assessed using hematoxylin and eosin staining, echocardiography, electrocardiogram, quantitative RT-PCR, Western blotting, [3H]-ryanodine binding assay, confocal microscope, ROS dye Amplex Red and 2′,7′-dichlorofluorescein diacetate. RyR1 OE mice had increased whole heart, left ventricular weight, and left ventricular wall thickness, but decreased cardiac output and …
Choosing A Primary Care Provider: A Formative Qualitative Study To Identify Patient Preferences, Chris Gillette, Jan Ostermann, Sarah O'Neal, Aylin A. Aguilar, Kandice Lacci-Reilly, Jessica Valente, Chris Everett, Robin Gibson, Sonia Crandall, Laiton Steele
Choosing A Primary Care Provider: A Formative Qualitative Study To Identify Patient Preferences, Chris Gillette, Jan Ostermann, Sarah O'Neal, Aylin A. Aguilar, Kandice Lacci-Reilly, Jessica Valente, Chris Everett, Robin Gibson, Sonia Crandall, Laiton Steele
Journal of Patient-Centered Research and Reviews
Purpose: (1) Identify what characteristics patients evaluate when choosing their primary care provider (PCP), (2) characterize how provider characteristics factor into patients’ choice of PCP, and (3) develop a list of features for a quantitative preference study in the form of a discrete choice experiment (DCE).
Methods: We used a cross-sectional, qualitative study to accomplish the objectives. We recruited participants from an internal medicine clinic and the general public in North Carolina. We used convenience and purposive sampling to recruit participants as a single sample. All participants completed a semistructured interview. We then used a reflexive thematic analysis to analyze …
Paving The Way In New Unh Supply Chain Management Program, Aaron Sanborn
Paving The Way In New Unh Supply Chain Management Program, Aaron Sanborn
UNH Today Archive
No abstract provided.
Incorporating The Use Of Puppets And Puppetry-Based Approaches In Nutrition: A Scoping Review Of Interventions, Basil H. Aboul-Enein, Nada Benajiba, Majd Jan, Sarah Ali Alasmari, Patricia J. Kelly, Stephen F. Gambescia, Elizabeth Dodge
Incorporating The Use Of Puppets And Puppetry-Based Approaches In Nutrition: A Scoping Review Of Interventions, Basil H. Aboul-Enein, Nada Benajiba, Majd Jan, Sarah Ali Alasmari, Patricia J. Kelly, Stephen F. Gambescia, Elizabeth Dodge
College of Nursing Faculty Papers & Presentations
Puppetry-based activities could serve as a low cost and manageable intervention in improving health-related outcomes. This review aims to identify the research to date and gaps in practice of the role and application of puppetry interventions in nutrition education. A scoping review was conducted using the PICOS framework and PRISMA-ScR guidelines, identifying studies across eight databases between January, 1980 and July, 2025.Twenty-five studies were identified, with the majority (n = 19) aimed at pre-school and school-aged children. Fifteen studies used quasi-experimental, pre-post designs. The use of hand and finger puppets was common, as well as videos and puppet theatre. …
Leveraging Mobile Health To Prevent Coronary Artery Disease Through Physical Activity Promotion Among Underserved Youth, Alina Yang, Jessh M. Mavoungou, Matthew C. Miller, Zarmina Amin, Zan Gao
Leveraging Mobile Health To Prevent Coronary Artery Disease Through Physical Activity Promotion Among Underserved Youth, Alina Yang, Jessh M. Mavoungou, Matthew C. Miller, Zarmina Amin, Zan Gao
International Journal of Physical Activity and Health
Coronary artery disease (CAD) develops earlier than often recognized, with underserved youth facing disproportionate cardiometabolic risk. Low participation in moderate-to-vigorous physical activity (PA) is a key driver. This viewpoint explores how mobile health (mHealth) technologies can serve as tools to increase PA engagement among underserved youth, guided by a combined Self-Determination Theory and RE-AIM (Reach, Effectiveness, Adoption, Implementation, and Maintenance) framework. It highlights current barriers to mHealth adoption, limited cultural relevance, engagement challenges, and accessibility gaps, and presents key considerations for designing culturally informed, age-tailored, and youth-centered mHealth interventions. Overall, the paper calls for a more inclusive and targeted approach …
The Mediating Effect Of Physical Activity On Mental Health Among Sexual Minority Adolescents During Covid-19, You Fu, Ryan D. Burns, Hayley Almes, Xiangren Yi, Nora Constantino
The Mediating Effect Of Physical Activity On Mental Health Among Sexual Minority Adolescents During Covid-19, You Fu, Ryan D. Burns, Hayley Almes, Xiangren Yi, Nora Constantino
International Journal of Physical Activity and Health
Purpose: Adolescents identifying as a sexual minority frequently experience mental health issues exacerbated by the COVID-19 pandemic. Physical activity (PA) has numerous benefits for mental health and emotional development. The purpose of this study was to examine the mediating effect of PA on the association between sexual minority status and mental health among adolescents during COVID-19. Methods: A sample of adolescents (grades 9-12) completed the online Adolescent Behaviors and Experiences Survey (ABES; N = 7677; 49.6% male) in 2021. The independent variable in this study was a recorded binary response item asking about sexual orientation. The dependent variable was a …
Climate Change Attributable Flooding And Post-Disaster Housing Market Dynamics: Evidence From Hurricane Harvey, Kaylynn Larrison
Climate Change Attributable Flooding And Post-Disaster Housing Market Dynamics: Evidence From Hurricane Harvey, Kaylynn Larrison
LSU Doctoral Dissertations
Climate change is increasing the frequency and severity of extreme weather events, yet the social consequences of climate change attributable impacts remain relatively underexplored. This dissertation examines whether flooding attributable to climate change was associated with distinct post-disaster recovery outcomes following Hurricane Harvey in Harris County, Texas. To explore this link, parcel-level flood model outputs are integrated with Harris County Appraisal District parcel data and OpenFEMA records on tract-level flood insurance policies and post-Harvey aid. Response variables are operationalized as change scores to capture differences between pre- and post-storm conditions and outcomes are measured over a five-year post-storm period. Across …
Evaluating The Impact Of A Standardized Intimate Partner Violence Screening Tool In A Clinical Setting, Dylan Bogucki
Evaluating The Impact Of A Standardized Intimate Partner Violence Screening Tool In A Clinical Setting, Dylan Bogucki
Evidence-Based Practice Project Reports
Intimate partner violence (IPV) is defined as any act of violence, including physical, sexual, stalking, or psychological aggression by an intimate partner, former or current. Despite being preventable, IPV remains a leading nonobstetric cause of morbidity and mortality among women, which highlights the need for systematic screening in clinical settings. The purpose of this quality improvement project is to identify women who have previously experienced or are currently experiencing IPV and to connect them with appropriate resources. Twenty-one studies were identified, all demonstrating moderate to high methodological quality and supporting the need for routine IPV screening in women’s health settings. …
Redefining Skincare For Sensitive Lives, Ashlyn G. Sierra Ms
Redefining Skincare For Sensitive Lives, Ashlyn G. Sierra Ms
Honors College Theses
This study outlines how a skincare company that is both ethically and effectively developed for people suffering from eczema could create accessible, affordable products as well as maintain its commitment to being inclusive. The study uses scientific data regarding eczema in order to examine why certain aspects of skin care are helpful or dangerous when it comes to individuals who suffer from eczema, in addition, it provides an assessment of current skin care product offerings that are designed for people with eczema. Using the Wise Leadership Framework, the study presents a hypothetical business plan for a new, eczema-based skin care …
The Lone Star Luminarias: 1880–1980, Dolores Atencio, Tracy C. Almanzán
The Lone Star Luminarias: 1880–1980, Dolores Atencio, Tracy C. Almanzán
The Scholar: St. Mary's Law Review on Race and Social Justice
The Lone Star Luminarias recovers and documents the lives, careers, and legal contributions of the first Latina lawyers licensed to practice law in the State of Texas. This article is a series of articles based upon the original research conducted by Visiting Scholar, Dolores Atencio (2016–2022) to identify and document the accomplishments of the first generation of Latina attorneys in the country, circa 1880–1980, Luminarias. The Luminarias Study involved archival research of 167 law
schools across the country, and a review of bar records, state bar records, past and contemporary news accounts, oral histories, and other records such as U.S. …
Left Behind: How The Ssi Program Fails To Support Young People With Disabilities, Caitlyn B. Acosta
Left Behind: How The Ssi Program Fails To Support Young People With Disabilities, Caitlyn B. Acosta
The Scholar: St. Mary's Law Review on Race and Social Justice
The Supplemental Security Income (SSI) Program was created to protect people with disabilities from extreme poverty. However, its outdated rules and punitive structures trap millions in cycles of poverty and exclusion. For young adults with disabilities, the SSI system worsens existing barriers by limiting income and savings, discouraging work, and fostering dependence instead of independence. Drawing on personal narratives, historical context, and current policies, this Comment highlights how SSI reflects a long legacy of ableism and systemic neglect. The stories of individuals who have been denied dignity, family stability, and community belonging illustrate that reform is not just about policy, …
Statutes Of Unjust Limitations: An Argument In Favor Of Increasing The Statute Of Limitations For Civil Cases Of Adult Sex Crimes In Texas, Athina E. Castañon
Statutes Of Unjust Limitations: An Argument In Favor Of Increasing The Statute Of Limitations For Civil Cases Of Adult Sex Crimes In Texas, Athina E. Castañon
The Scholar: St. Mary's Law Review on Race and Social Justice
This Comment argues that Texas’s current five-year statute of limitations for civil claims arising from adult sex crimes is unjust and misaligned with modern trauma research. While Texas has enacted meaningful legislation for child survivors, adult survivors who experience sexual violence after the age of eighteen remain subjected to an arbitrarily short filing window that fails to account for the psychological, social, and economic barriers to reporting and litigation. Drawing on historical context, trauma-informed research, and a comparative analysis of reform efforts, this Comment demonstrates how rigid statutes of limitations disproportionately harm survivors and undermine access to civil justice.
Development And Psychometric Analysis Of An Instrument To Assess Social Stigma Associated With Parkinson's Disease, Ana Lourdes Guerra-Anzaldo, Arturo Emmanuel Espinosa-Picos, Karen Itzel Sánchez-Ramírez, Ana Jimena Hernández-Medrano, Amin Cervantes-Arriaga, Ariadna Domínguez-García, Mayela Rodríguez-Violante
Development And Psychometric Analysis Of An Instrument To Assess Social Stigma Associated With Parkinson's Disease, Ana Lourdes Guerra-Anzaldo, Arturo Emmanuel Espinosa-Picos, Karen Itzel Sánchez-Ramírez, Ana Jimena Hernández-Medrano, Amin Cervantes-Arriaga, Ariadna Domínguez-García, Mayela Rodríguez-Violante
Journal of Patient-Centered Research and Reviews
Purpose: To develop and report the psychometric properties of a comprehensive scale designed to measure stereotypes and attitudes of the general population towards people with Parkinson’s disease (PwP).
Methods: The development and analysis of the Parkinson's Disease Stigma Perception scale (PDSP) included four phases: 1) literature review, 2) item development, 3) tests of validity and reliability, and 4) dissemination of a preliminary version. Internal consistency was assessed using Cronbach's alpha, exploratory factor analysis (EFA) to explore underlying structures, and confirmatory factor analysis to test the model's fit.
Results: Six (2%) of the 294 survey respondents were disqualified because of incomplete …
The Compassionate Heart Patient Questionnaire: Validation And Reliability Of A Novel Measure Of Relationship-Centered Care, Dustin Willis, Everett Lohman, Lida Gharibvand, Andrew Sullivan, Lee Berk
The Compassionate Heart Patient Questionnaire: Validation And Reliability Of A Novel Measure Of Relationship-Centered Care, Dustin Willis, Everett Lohman, Lida Gharibvand, Andrew Sullivan, Lee Berk
Journal of Patient-Centered Research and Reviews
Purpose: To establish the validity and reliability of the Compassionate HEART Patient Questionnaire (CHPQ) for measuring relationship-centered care and exploring associations between patient ratings and mood state changes in patients with persistent pain.
Methods: Thirty-nine adults with persistent pain (> 3 months) completed pre-/post-interaction Profile of Mood States assessments and rated providers using the Consultation and Relational Empathy (CARE) measure and the novel CHPQ following physical therapy evaluations. Psychometric properties were assessed through factor analysis, concurrent validity (correlation with CARE), and internal reliability (Cronbach's alpha). Pre-post mood changes were analyzed using Wilcoxon signed-rank test.
Results: The CHPQ demonstrated good internal …
Landscapes Of Home And Maps Of The Heart: Using Art To Understand The Meaning Of Home For First And Second Generation Immigrants In Worcester Massachusetts, Sophie Sundaram
Fenwick Scholar Program
Landscapes of Home and Maps of the Heart: Using Art to Understand the Meaning of Home for First and Second Generation Immigrants in Worcester Massachusetts explores how individuals with histories of migration understand and experience “home,” and how those understandings can be translated into visual form through portraiture. Developed through conversations with Worcester community members, the project combines studio practice with methodological approaches drawn from sociological and anthropological fieldwork, including participant interviews, relational research practices, and community-based engagement.
Grounded in Worcester, a city shaped by multiple waves of migration and cultural diversity, the project investigates how senses of home are …
Oregon State Rank Assessment For Wiens' Dwarf Mistletoe (Arceuthobium Abietinum Ssp. Wiensii), Lindsey K. Wise
Oregon State Rank Assessment For Wiens' Dwarf Mistletoe (Arceuthobium Abietinum Ssp. Wiensii), Lindsey K. Wise
Institute for Natural Resources Publications
Oregon state conservation status assessment for Wiens' dwarf mistletoe (Arceuthobium abietinum ssp. wiensii) using NatureServe methodology, 2026.
Investigation Of New Surfaces For Pressure Injury Prevention, Jeremy Choh
Investigation Of New Surfaces For Pressure Injury Prevention, Jeremy Choh
Mechanical Engineering and Materials Science Independent Study
Pressure injuries remain as a large challenge for the health care industry with hospitals responsible for more than half of the pressure injuries cases. [1] Current methods for pressure injury prevention are labor intensive or expensive. The use of hydrogels and diaelectric materials with reversible mechanical properties were investigated to generate 32 mm Hg pressure relief for pressure injury prevention. However, it was determined that the mechanical properties of these materials were inefficient in mechanical strength, activation time, or activation energy to be suitable for health care purposes. The use of low intensity ultrasound therapy was studied instead. A single …
The Impact Of Trump Administration Immigration Policies On Connecticut Educators And Families, Elena C. Bielesz
The Impact Of Trump Administration Immigration Policies On Connecticut Educators And Families, Elena C. Bielesz
Honors Scholar Theses
Anti-immigrant rhetoric, policies, and practices, which have grown increasingly prevalent due to the current national political climate, have adverse effects on the mental health of immigrant families and the teachers that support them in Connecticut. This study seeks to represent the opinions of Connecticut K-12 MLL (Multilingual Learners) Education Teachers and Administrators whose daily lives are impacted by the anti-immigrant and anti-refugee policies and procedures. The study employed a human subjects-based approach supported by a review of related literature. MLL Educators from around the state of Connecticut were interviewed. These interviews found that while immigration-based trauma and stressors are common …
Volume Electron Microscopy Reveals Heterogeneity Of The Hemostatic Response In Veins And Arteries, Maurizio Tomaiuolo, Meghan E. Roberts, Jenna R. Severa, Christopher D. Mansi, Brenna B. Y. Mathers, Anna Mannix, Trace A Christensen, Lawrence F Brass, Talid Sinno, Timothy J. Stalker
Volume Electron Microscopy Reveals Heterogeneity Of The Hemostatic Response In Veins And Arteries, Maurizio Tomaiuolo, Meghan E. Roberts, Jenna R. Severa, Christopher D. Mansi, Brenna B. Y. Mathers, Anna Mannix, Trace A Christensen, Lawrence F Brass, Talid Sinno, Timothy J. Stalker
Cardeza Foundation for Hematologic Research
Intravital imaging studies have provided insights into the spatial and temporal variations of platelet activation and thrombin generation that occur during hemostasis; however, these studies are generally limited to small vessels due to the practical limitations of imaging in thicker tissues. Recent advances in cleared tissue fluorescence imaging as well as volume electron microscopy (vEM) coupled with machine learning-based image segmentation provide an opportunity for analysis of the 3-dimensional structure of complex tissues. We utilized these technologies to examine hemostatic plugs from murine jugular veins and carotid arteries to investigate the spatial distribution of platelet activation and biochemical responses in …