Digital Commons Network^™

Mapping The Conformational Landscape Of Dna Minicircles Through Atomic Force Microscopy And Shape Space Analysis, Laura Wiggins, Tobias A Firth, Max C Gamill, Thomas E Catley, Agnes Noy, Jonathan M Fogg, Lynn Zechiedrich, Alice L B Pyne

Faculty, Staff and Students Publications

The structural dynamics of DNA underpin essential biological processes, yet conventional structural biology methods often obscure conformational heterogeneity through ensemble averaging. Atomic force microscopy (AFM) provides single-molecule topographical maps capable of capturing both local and global variation, but extracting quantitative insight from these images remains challenging. Here, we introduce an automated framework that reduces AFM data to spline representations of the DNA backbone and applies cyclic Procrustes analysis to quantify shape similarity across ensembles. Using purified topoisomers of 339 bp DNA minicircles ranging from relaxed to highly negatively supercoiled, we resolved and measured the relative abundance of conformational states across …

Human Exposure To Spirochete-Infected Ornithodoros Turicata In A Residential Setting In The Austin, Texas Metropolitan Area, Serhii Filatov, Bonny Mayes, Sarah M Gunter, Alexander R Kneubehl, Job E Lopez

Faculty, Staff and Students Publications

A central Texas resident found an engorged Ornithodoros turicata tick attached to their ankle while at home. The tick tested positive for Borrelia turicatae, an agent for soft tick-borne relapsing fever. Three weeks earlier, the patient started experiencing febrile episodes, rash, and nausea, yet the etiological agent went undiagnosed.

Na-Gst-1 Adsorbed On Alhydrogel Co-Administered With Different Toll-Like Receptor Agonists In Hookworm-Naive Adults Using A Controlled Human Infection Model In The Usa: A Phase 2, Double-Blind, Randomised Controlled Trial, Caroline K Dirosato, Elissa M Malkin, Sean M Lee, Guacyara Erwin, Lara Hoeweler, Larissa Scholte, Maria Elena Bottazzi, David I Pritchard, Peter J Hotez, Jeffrey M Bethony, David J Diemert

Faculty, Staff and Students Publications

Background: Necator americanus glutathione S-transferase-1 (Na-GST-1) performs a crucial enzymatic step in hookworm blood feeding and is a leading target for vaccine development. Phase 1 trials in Brazil, Gabon, and the USA of recombinant Na-GST-1 adsorbed on Alhydrogel (Na-GST-1/Al), co-administered with or without AP 10-701 (Toll-like receptor [TLR] 4 agonist) or CpG 10104 (TLR9 agonist), showed the vaccine was well tolerated, safe, and immunogenic. A controlled human hookworm infection (CHHI) model was previously developed to evaluate the effect of candidate hookworm vaccines on infection. We aimed to assess the effect of vaccination with three different Na-GST-1 vaccine formulations on subsequent …

Blinded, Bias-Controlled Multi-Rater Evaluation Of Human-Versus-Ai Brain Metastasis Segmentation Using A Hybrid Foundation-Model Framework, Yiding Han, Enze Zhu, Mhd Hasan Ai Mekdash, Omar Awad, Piyush Pathak, Shixiao Liang, Daniel Allan Hamstra, Xizhe Zhang, Zaid Ali Siddiqui, Baozhou Sun

Faculty, Staff and Students Publications

Background: Accurate segmentation of brain metastases (BM) is essential for diagnosis, stereotactic radiosurgery planning, and longitudinal assessment. However, manual contouring is time-intensive, limiting clinical scalability, and exhibits substantial inter-observer variability. This variability complicates objective assessment of automated segmentation methods and challenges interpretation of model performance.

Purpose: To address these limitations, we developed TUM-SAM, a hybrid foundation-model framework for fully automated BM segmentation, and introduced a bias-controlled, blinded multi-rater evaluation paradigm to determine whether AI-based BM segmentation has reached expert-level performance and whether AI-generated contours are preferred by human experts under unbiased assessment.

Methods: TUM-SAM integrates nnU-Net-based lesion detection with a …

Quality Domains In Home-Based Pediatric Hospice And Palliative Care: Adolescent And Young Adult Perspectives, Daniel H Grossoehme, Claire A Crawford, Jhansi Chandra Ellakula, Toluwalase Ajayi, Justin N Baker, Sarah Friebert, Lisa Humphrey, Jillann Jarrell, Rachel Thienprayoon, Pamela S Hinds

Faculty, Staff and Students Publications

Background: Current United States palliative care practice guidelines are intended to cover care across all age groups. Previous research with pediatric providers and caregivers concluded that pediatric hospice and palliative care have unique attributes. Not known is how adolescent hospice and palliative care patients understand quality palliative care. The purpose of this study was to characterize and define quality of care domains as described by adolescent and young adult patients receiving home-based hospice and palliative care.

Methods: Semi-structured interviews with 10-26 year-olds who received home-based hospice and/or palliative care visits in the prior three years at six diverse sites in …

Biphasic Sleep Is A Feature Of Sleep Disturbance In Syngap1-Rd, Aida Doucoure, Het Patel, Mark A Abboud, Alice Sperry, Sarah K Wanigatunga, Deana Crocetti, Heather Volk, Adam P Spira, Stewart H Mostofsky, Vaishnav Krishnan, Constance Smith-Hicks

Faculty, Staff and Students Publications

Study objectives: Sleep problems are common in children with SYNGAP1-Related Disorder (SYNGAP1-RD). The use of devices that objectively estimate sleep are complicated by co-occurring sensory disorders in this population. We examined the feasibility and validity of wrist actigraphy to examine sleep and rest-activity rhythms (RAR).

Methods: Data from five children with SYNGAP1-RD and 42 typically developing children were analyzed. All children were asked to wear the Actiwatch-2 for 14 continuous days and caregivers were asked to complete a sleep diary and the Children Sleep Health Questionnaire (CSHQ). Parametric (alpha, beta, acrophase, amplitude, up/down mesor, mesor), nonparametric (intradaily variability, interdaily stability, …

Development And Evaluation Of An Automated Report-Based Chart Checking Tool In External Beam Radiotherapy, Andrew Wroe

All Publications

Background: Computerized radiotherapy chart checking tools have revolutionized the initial physics chart review process as they offer verification of large amounts of plan parameters in seconds and allow physicists to concentrate on high-skill-level items that are challenging for automation. Both commercialized and in-house chart check solutions typically rely on access to the patient data within the live SQL database of the radiation oncology information system (ROS). This is a complex task potentially posing risks to both database integrity and overall system performance when accessed during clinic operation hours.

Purpose: The aim of this study was to develop and test a …

Thermal Transport Tuning Via Nanoscale Hierarchical Frameworks For Fe2val Thermoelectric Materials, Apoorva Pradip Joshi

Dartmouth College Master’s Theses

Waste heat represents a vast, untapped energy resource. Thermoelectric materials offer a promising route to harvest this energy by directly converting thermal gradients into electricity. The Heusler alloy Fe2VAl is a prime candidate for such applications because it is non-toxic and cost-effective. However, its intrinsically-high thermal conductivity severely limits performance. A detailed understanding of thermal transport mechanisms is therefore essential for improving its thermoelectric efficiency. This work examines the effect of atomic disorder on the thermal transport behaviour of Fe2VAl. We use germanium doping to introduce atomic-scale disorder and demonstrate a substantial reduction in thermal conductivity, from 28 W/m-K in …

Animal Assisted Interventions For Older Adults Experiencing Cognitive Impairment, Preeti Bachu

Psychology

As individuals age, they often experience gradual declines in cognitive abilities that ultimately affect their quality of life and independence. Severe cognitive decline is not necessarily a natural or universal part of the aging process and tends to be exacerbated by various neurodegenerative diseases, most notably Alzheimer's disease (Colita et al., 2024). There have been promising outcomes of animal assisted interventions in improving the conditions of several populations and spaces, including educational, rehabilitative, and therapeutic settings. Animal assisted therapy specifically is delivered by a qualified or licensed health professional. Previous research has shown a large benefit in long-term memory, verbal …

Examining The Effectiveness Of Nature Metaphor Patterns For Cultivating Intimacy, Acceptance, Relationship Satisfaction, Self-Compassion, And Positive Communication In Couples, Jenna Rice, Sarah Fraher, Elizabeth Engelkamp, Melissa Mcternan, Hannah Grigorian, James Cordova

Psychology

Effective couple interventions, such as Integrative Behavioral Couple Therapy and the Relationship Checkup, include the labeling of common relationship patterns. This study hypothesized that a micro-intervention using nature metaphors, such as “Porcupine–Turtle,” would produce greater improvements in relationship health relative to descriptive labels such as “Approach–Withdraw.” Twenty-five couples were presented with nature metaphor patterns, while 24 couples were presented with descriptive patterns. Several measures of relationship health showed significant improvements across both groups, while other measures showed significant improvements for descriptive patterns only. Generally, nature metaphor patterns were initially rated as more novel and more resonant. Broadly, these results add …

Improving Urban Search And Rescue Team Coordination Through Adaptive Context Awareness, Daniel Reyes Duran

Doctoral Dissertations and Master's Theses

Modern multi-agent Urban Search and Rescue (USAR) operations heavily rely on mobile geospatial Common Operating Pictures (COPs) to maintain team coordination and Situational Awareness (SA). However, the proliferation of high-frequency sensor telemetry at the tactical edge has introduced a data saturation paradox challenge: while information theoretically drives informed decision-making, unmanaged data surges induce increased operator cognitive overload and alert fatigue on mobile End-User Devices (EUDs), while downstream data-broadcasting models inherently strain edge processing and viewport environments.

To resolve these constraints, this dissertation presents a context-aware Value of Information (VoI) data-management framework integrated directly with a custom, event-driven Android Team Awareness …

Polarized Truth Decay & The Role Of Education In A Democracy, Sonja Varbelow, William Yaworsky

Anthropology Faculty Publications

The purpose of this paper is to delineate the causes and dangers of truth decay for a democracy, the role education has played in the process, and how education can present a resistance to this dangerous trend. Truth decay has been characterized as an increase in quantity of opinion over fact, divergent views on what constitutes a fact, and decreased confidence of traditional sources of information. We agree that truth decay is a serious problem but would characterize it somewhat differently. Opinions, divergent views, and decreased confidence in traditional sources of information are not detrimental by themselves. Instead, we see …

Highly Confident But Slightly Uncertain: A Long Overdue Reinvention Of The School Scientific Method, Uncertainty And Error - Volume 3, Stephen Demeo

Publications and Research

Volume 3 explores the essential roles of uncertainty and error in scientific experimentation. It distinguishes between scientific error and scientific uncertainty: Scientific error refers to the difference between an experimental value and an accepted literature value, as well as the difference between data points in an x-y scatter plot and a predicted trendline. Scientific uncertainty, on the other hand, is associated with doubt arising from instrument limitations, variability within a data set, and psychological factors. Human error is set apart as mistakes or blunders, not to be conflated with scientific error.

Of utmost importance to the scientific investigator is the …

Highly Confident But Slightly Uncertain: A Long Overdue Reinvention Of The School Scientific Method, The X - Volume 1, Stephen Demeo

Publications and Research

In Volume 1, I critique the traditional School Scientific Method—an oversimplified, underdeveloped, and short, stepwise process—and argue for a more authentic, detailed, and rigorous approach to experiment-based scientific inquiry in education. I briefly explain the historical roots of the School Scientific Method, its benefits in increasing the exposure of experimental science to the masses, and its limitations in fostering more authentic scientific thinking and experimental practice.

In place of this traditional and problematic method, I introduce a graphic organizer called sciXpsy to foster both big-picture and detailed scientific thinking. The heuristic combines scientific concepts and methodology with the psychology of …

Highly Confident But Slightly Uncertain: A Long Overdue Reinvention Of The School Scientific Method, Confidence - Volume 2, Stephen Demeo

Publications and Research

Focusing on the psychology of empirical science, Volume 2 is an in-depth exploration of how students can build confidence in scientific data. Central to the text are twelve Confidence Indicators, a comprehensive set of approachable strategies and methodologies that help investigators produce, evaluate, and argue for reliable and valid empirical data. They include:

Good (and Ethical) Laboratory Practices

Repetition

Peer Replication/Review

Secondary Tests

Comparisons with Standards

Plots and Patterns

Conceptual Support

External Data Sources

Precision

Accuracy

Checking Calculations

Redesign/What-If Ideas

This volume includes numerous school science experiments that demonstrate how these indicators are applied across a wide range of educational …

A Systems Analysis Of Autonomous Uav Swarm Spraying For Precision Agriculture, Ryker Davis

Graduate Theses and Dissertations

The application of Unmanned Aerial Vehicles (UAVs) in civilian sectors, particularly for precision agriculture and targeted aerial spraying, has rapidly evolved from passive monitoring to advanced, autonomous, multi-agent coordination. While deploying UAV swarms offers a promising solution to the payload and battery endurance limitations of single drone operations, realizing field-scale coverage introduces significant logistical and safety challenges. To evaluate the trajectory and viability of swarms, this study synthesizes bibliometric trends, operational simulation, and safety reporting analysis. First, because physical testing of complex swarms poses financial and safety risks, we developed an agent-based and discrete event simulation to evaluate autonomous spraying …

Design And Modeling Of A Robotic Manipulator For Agricultural Explorations, Trent Thomas Powell

Graduate Theses and Dissertations

This thesis focuses on the modeling, design, and early testing of a spherical revolute revolute prismatic (RRP) robotic manipulator for probe based applications. The system is motivated by agricultural grain probing, where a probe is positioned over a truck bed and inserted into the grain to collect samples as trucks enter storage facilities, ports, hubs, or transfer points. A spherical RRP configuration is useful for this type of task because it provides a large accessible workspace while maintaining a relatively simple mechanical structure. A model based design approach is used to develop the system, beginning with forward and inverse kinematics …

#Metoo Vs. Anti-Dei, Nicole Porter

Faculty Publications

Nine years ago, the entire country was talking about the #MeToo movement, which can be credited with the unseating of hundreds of powerful men and changing the way we talk about and address sexual harassment in the workplace. In 2026, continued progress of the #MeToo movement might be threatened by a new movement-the Trump Administration's attack on Diversity, Equity, and Inclusion (DEI) initiatives. This article discusses the ways in which the #MeToo movement conflicts with the anti-DEI movement. Specifically, it addresses how employers will respond to pressure to dismantle DEI programs, offices, and training, and how those responses will affect …

Video Games As An Advertising Media: A Comparative Analysis Between Portuguese And Spanish University Students, Alejandro Tapia-Frade, Gema Bonales-Daimiel, Belén Moreno-Albarracín

Faculty Research, Scholarly, and Creative Activity

Changes in leisure consumption are causing transformations in the relationships between young people and brands. A reflection of this is found in video games, whose predominant user profile is that of young people between 18 and 30 years old. They invest more and more time in digital universes: they play, socialize and even buy, and income from the video game sector has increased year after year in Spain and in the world, surpassing those generated jointly by cinema and music. A review of previous literature indicates the relevance as an object of study of video games that are used as …

Replacing Surrogate Decision-Making With Supported Decision-Making For Minimal Risk Research, Jennifer Hawkins, Aaron Eli Segal, David Wendler, Dana Howard

Faculty and Staff Publications

We advocate for a change in the way individuals with cognitive impairment are enrolled in minimal risk clinical research. We do so in the hope that such a change will lead to more cognitively impaired individuals being enrolled in research. Our proposal applies only to cases where would-be participants retain some interest in decision-making as well as the ability to express a decision. In these cases, we argue that the common practice whereby researchers either obtain consent from the individual or consent from a surrogate and assent from the individual, is ethically unnecessary. Instead, a process of facilitated decision-making based …

Spiritual Minimalism: Analytical Studies Of Selected Piano Works By Pärt, Górecki, And Tavener, Boran Zhao

Theses and Dissertations

This dissertation examines selected solo piano works by Arvo Pärt, Henryk Górecki, and John Tavener to explore how theological influences are reflected through their distinctive compositional languages. Current studies show that scholars primarily concentrate on their choral and orchestral repertoire, while their piano works have not received enough attention. This study argues that their solo piano works carry the same of theological influence found in their choral and orchestral compositions.

Through historical, analytical, and theological approaches, the dissertation explores how their compositional techniques convey spiritual meanings in selected solo piano pieces. Pärt’s Für Alina and Variations for the Healing of …

Bridging Theory And Practice: Examining The Relationship Between Developmental Knowledge, Self-Efficacy For Emotion Support, And Teachers’ Responses To Children’S Emotions In The Classroom, Shea Elizabeth Ferguson

Theses and Dissertations

How teachers respond to children’s emotions in the classroom plays a central role in shaping children’s emotional development, a process known as emotion socialization. Although research has linked teachers’ emotion socialization responses and practices to important child outcomes, less is known about the factors that underlie teachers’ responses to children’s emotions. Given the importance of the early developmental years for building foundational skills important for long-term success, the focus of this study was on those working with early childhood and elementary aged children. Accordingly, this mixed methods study examined preservice teachers’ (1) developmental knowledge and (2) self-efficacy for supporting children’s …

Investigation Of Early To Late Cretaceous North American Ecosystems And Paleoclimate Using Geochemical Proxies, Clayton William Forster

Graduate Theses and Dissertations

The relationships between the environment and an organism can be traced through stable isotope and trace element geochemistry. This thesis explores the stable oxygen and carbon isotopic composition of fossil vertebrate assemblages in pursuit of revealing the relationships between dinosaurs and their archosaur relatives with the environment in which they lived during the Cretaceous. The Cretaceous Period of North America experienced some of the hottest intervals in the Phanerozoic Eon and the continental vertebrate taxa that existed during this time recorded these periods within the stable isotopic composition of their skeletal bioapatite. Oxygen isotopes in the bioapatite of vertebrates reflect …

Item Response Theory Evaluation Of Mental Health Sentiment Scales, Muhammad Amzad Hossain

Graduate Theses and Dissertations

Mental health is an essential part of overall well-being. Accurate measurement of constructs such as loneliness, anxiety, depression, and life satisfaction is extremely important in research and practice. Traditional approaches based on Classical Test Theory (CTT) are limited. They rely on total test scores and assume constant measurement error across individuals. They may not reflect true variability in measurement precision (Zein & Akhtar, 2025; Marvianto, 2023). In contrast, Item Response Theory (IRT) provides an advanced approach by examining item-level performance. It models the relationship between latent traits and responses (Zein & Akhtar, 2025 ). To address these limitations, this study …

Identifying Muscle Health Deficits Among Older Adults With And Without Cognitive Impairment, Ray Guerreros Urbina

Graduate Theses and Dissertations

Age-related declines in skeletal muscle health (SMH) and cognitive function are major contributors to disability, loss of independence, and reduced quality of life among older adults. Emerging evidence suggests that skeletal muscle and cognitive health may be interconnected through shared physiological mechanisms; however, the relationships between specific aspects of SMH and cognitive performance remain unclear. Therefore, the purpose of this study was to examine the relationships between SMH and cognitive performance among older adults and to determine whether SMH differs between cognitively healthy and cognitively impaired individuals after accounting for age and physical activity. A cross-sectional analysis was conducted using …

Challenges And Opportunities In Dry Aging: Producer Perspectives And Valorization Of Dry- Aged Waste Products, Peyton Scout Arnold

Graduate Theses and Dissertations

Despite the projected increase in the dry aging market, producers may be hesitant to incorporate dry aging practices. This thesis aims to identify primary concerns faced by producers in implementing a dry aging program. Semi-structured interview surveys were conducted via Qualtrics (N = 100). Results indicated that an “advanced” level of dry aging knowledge correlated with a higher importance in the use/sale of dry aged products (P = 0.02). Cost, demand, and sourcing were the top three concerns of producers practicing dry aging. Those considering implementation were concerned with cost, processing parameters, safety, demand, and sourcing. No significant differences were …

The Narrative Dynamics Of Special Books By Special Kids Youtube Videos And Comments: A Study Of Disability Rhetoric And Public Narrative Engagement, Jessica Louise Allee

Graduate Theses and Dissertations

Digital media has expanded disability advocacy and community building by enabling accessible, visual storytelling and connection. Special Books by Special Kids (SBSK), a YouTube channel using interview-based disability storytelling, foregrounds people’s experiences with disabilities in the videos, while inviting viewers to interpret, identify with, and respond in the comments. As a participatory storytelling space, SBSK offers a site for examining how disability meanings are constructed, negotiated, and transformed through narratives, multimodal practices, and audience uptake, while revealing how dominant disability rhetoric or ableist discourse that positions people with disabilities as burdens, inherently deficient, or socially devalued, can be reproduced, complicated, …

Development Of Medical Device For Hypovolemic Assessment Using Peripheral Venous Pressure, Connor J. Breshears

Graduate Theses and Dissertations

Accurate assessment of intravascular volume status remains challenging because traditional measurements such as heart rate, blood pressure, physical examination findings, and laboratory values may be subjective, delayed, or insensitive to early volume loss. Peripheral venous pressure (PVP) waveform analysis provides a potential approach for minimally invasive volume-status monitoring because PVP waveforms can be acquired from peripheral venous access and may contain frequency-domain information related to cardiac activity, respiratory modulation, vascular coupling, and intravascular filling. This thesis presents the development and evaluation of a proof-of-concept embedded prototype for PVP waveform acquisition and volume-status classification. The system integrates a Millar MEMS pressure …

A Quantitative Examination Of The Relationship Between College Gpa And Culturally Inclusive Support Services, Sara Jo Barnett Nsakashalo

Graduate Theses and Dissertations

The Eurocentric formal education model was first introduced to American Indians as a weapon, a tool by which American Indian culture and lifeways could be dismantled thus forcing the entire population into assimilation. The residual trauma of the Indian Boarding School era and other federal Indian policy continue to impact American Indian students, even at the postsecondary level. For decades, research has focused on exploring the role of Tribal Colleges and Universities (TCU) to explain the increased success American Indian students experience at these institutions when compared to other institutions. Though they have demonstrated an exceptional ability to adequately serve …

Evaluation Of Metabolizable Energy And Phosphorus Availability In Conventional And Fractionated Dry-Grind Corn Co-Products For Poultry, Luke Parisi

Graduate Theses and Dissertations

The purpose of this research was to determine the availability of energy and phosphorous (P) in dry-grind corn co-products in chickens was assessed. In the first objective, the determination of energy availability, both a precision-fed rooster assay and an ad libitum fed broiler chicken assay were used in order to elucidate energy availability in corn co-products. There were 8 samples of corn fermented protein (CFP) and 2 samples of conventional distillers dried grains with solubles (CDDGS) evaluated for metabolizable energy (ME). The CFP contained a mean CP and crude fat of 55 and 3.7% (DM basis), respectively, compared with 30 …