Digital Commons Network^™

Powering Single-Cell Genomics To Unravel Circulating Tumour Cell Subpopulations In Non-Small Cell Lung Cancer Patients, Emmanuel Acheampong, Michael Morici, Afaf Abed, Samantha Bowyer, Du-Bois Asante, Weitao Lin, Michael Millward, Elin S. Gray, Aaron Beasley

Research outputs 2022 to 2026

Background

Circulating tumour cells (CTCs) are attractive “liquid biopsy” candidates that could provide insights into the different phenotypes of tumours present within a patient. The epithelial-to-mesenchymal transition (EMT) of CTCs is considered a critical step in tumour metastasis; however, it may confound traditional epithelial feature-based CTC isolation and detection. We applied single-cell copy number alteration (CNA) analysis for the identification of genomic alterations to confirm the neoplastic nature of circulating cells with only mesenchymal phenotypes.

Methods

We isolated CTCs from blood samples collected from 46 NSCLC patients using the Parsortix system. Enriched cells were subjected to immunofluorescent staining for CTC …

The Impact Of Sleep-Wake Behaviour On Tennis Match Performance In Junior State Grade Tennis Players, Mitchell Turner, Philipp Beranek, Ian Dunican, Travis Cruickshank

Research outputs 2022 to 2026

Purpose:

To date, no study has investigated the extent to which sleep-wake behaviour (SWB) influences match performance in junior tennis players. This study aimed to assess the influence of SWB for the week and night before on match performance, particularly match analytics and activity.

Methods:

This study recruited 10 junior state grade tennis players who wore an actigraphy device and completed a sleep diary for the week before their match on two separate occasions throughout their competition season. Players wore a global positioning system device to track their movement during matches, and an experienced tennis coach recorded players' match analytics. …

January 2023 News Releases, University Of Montana--Missoula. Office Of University Relations

University of Montana News Releases, 1928, 1956-present

No abstract provided.

Transcriptomic Profiles Of Multiple Organ Dysfunction Syndrome Phenotypes In Pediatric Critical Influenza, Tanya Novak, Jeremy Chase Crawford, Georg Hahn, Mark W Hall, Simone A Thair, Margaret M Newhams, Janet Chou, Peter M Mourani, Keiko M Tarquinio, Barry Markovitz, Laura L Loftis, Scott L Weiss, Renee Higgerson, Adam J Schwarz, Neethi P Pinto, Neal J Thomas, Rainer G Gedeit, Ronald C Sanders, Sidharth Mahapatra, Bria M Coates, Natalie Z Cvijanovich, Kate G Ackerman, David W Tellez, Patrick Mcquillen, Stephen C Kurachek, Steven L Shein, Christoph Lange, Paul G Thomas, Adrienne G Randolph

Faculty, Staff and Students Publications

BACKGROUND: Influenza virus is responsible for a large global burden of disease, especially in children. Multiple Organ Dysfunction Syndrome (MODS) is a life-threatening and fatal complication of severe influenza infection.

METHODS: We measured RNA expression of 469 biologically plausible candidate genes in children admitted to North American pediatric intensive care units with severe influenza virus infection with and without MODS. Whole blood samples from 191 influenza-infected children (median age 6.4 years, IQR: 2.2, 11) were collected a median of 27 hours following admission; for 45 children a second blood sample was collected approximately seven days later. Extracted RNA was hybridized …

Case Report: Selexipag In Pediatric Pulmonary Hypertension: Initiation, Transition, And Titration, Jenna M Faircloth, Neelam D Bhatt, Corey A Chartan, Ryan D Coleman, Natalie Villafranco, Fadel E Ruiz, Raysa Morales-Demori, Elise Whalen, Erin Ely, Rozmeen Fombin, Nidhy P Varghese

Faculty, Staff and Students Publications

Selexipag, a selective prostacyclin receptor agonist, is approved for treating pulmonary arterial hypertension in WHO Group 1 adult patients. Compared to parenteral prostacyclin formulations, selexipag offers a significant improvement in patient’s and caregiver’s quality of life because of its oral formulation, frequency of administration, and mechanism of action. Although experience in the pediatric population is limited and selexipag is not FDA-approved for use in the pediatric pulmonary hypertension population, many US pediatric centers are expanding the use of this therapy to this younger population. We report our institution's experience in the use of selexipag to treat pulmonary hypertension in children …

Corrigendum: Case Report: Selexipag In Pediatric Pulmonary Hypertension: Initiation, Transition, And Titration, Jenna M Faircloth, Neelam D Bhatt, Corey A Chartan, Ryan D Coleman, Natalie Villafranco, Fadel E Ruiz, Raysa Morales-Demori, Elise Whalen, Erin Ely, Rozmeen Fombin, Nidhy P Varghese

Faculty, Staff and Students Publications

This corrects the article "Case Report: Selexipag in pediatric pulmonary hypertension: Initiation, transition, and titration", 1050508.

Characteristics Of Type 2 Diabetes In Female And Male Youth, Beatriz Castillo Rodriguez, Marcela Astudillo, Mustafa Tosur, Ahmad Rafaey, Siripoom Mckay, Fida Bacha, Ashok Balasubramanyam, Maria J Redondo

Faculty, Staff and Students Publications

The incidence of type 2 diabetes in children is rising and carries a worse prognosis than in adults. The influence of sex on pediatric type 2 diabetes outcomes has not been well investigated. We studied 715 youth with type 2 diabetes diagnosed at a median age of 13.7 years and compared sex differences in demographic, clinical, and laboratory characteristics within the first year of diagnosis. Females diagnosed with type 2 diabetes were younger and at a higher stage of pubertal development than males, yet presented with lower A1Cs, a lower prevalence of diabetic ketoacidosis, and higher HDL cholesterol levels.

Clinical Characterization Of Data-Driven Diabetes Clusters Of Pediatric Type 2 Diabetes, Mahsan Abbasi, Mustafa Tosur, Marcela Astudillo, Ahmad Refaey, Ashutosh Sabharwal, Maria J Redondo

Faculty, Staff and Students Publications

BACKGROUND: Pediatric Type 2 diabetes (T2D) is highly heterogeneous. Previous reports on adult-onset diabetes demonstrated the existence of diabetes clusters. Therefore, we set out to identify unique diabetes subgroups with distinct characteristics among youth with T2D using commonly available demographic, clinical, and biochemical data.

METHODS: We performed data-driven cluster analysis (K-prototypes clustering) to characterize diabetes subtypes in pediatrics using a dataset with 722 children and adolescents with autoantibody-negative T2D. The six variables included in our analysis were sex, race/ethnicity, age, BMI

RESULTS: We identified five distinct clusters of pediatric T2D, with different features, treatment regimens and risk of diabetes complications: …

Cholestasis Impairs Gut Microbiota Development And Bile Salt Hydrolase Activity In Preterm Neonates, Lauren E Lynch, Amy B Hair, Krishnakant G Soni, Heeju Yang, Laura A Gollins, Monica Narvaez-Rivas, Kenneth D R Setchell, Geoffrey A Preidis

Faculty, Staff and Students Publications

Cholestasis refers to impaired bile flow from the liver to the intestine. In neonates, cholestasis causes poor growth and may progress to liver failure and death. Normal bile flow requires an intact liver-gut-microbiome axis, whereby liver-derived primary bile acids are transformed into secondary bile acids. Microbial bile salt hydrolase (BSH) enzymes are responsible for the first step, deconjugating glycine- and taurine-conjugated primary bile acids. Cholestatic neonates often are treated with the potent choleretic bile acid ursodeoxycholic acid (UDCA), although interactions between UDCA, gut microbes, and other bile acids are poorly understood. To gain insight into how the liver-gut-microbiome axis develops …

Insulin-Like Growth Factor 1 Supplementation Supports Motor Coordination And Affects Myelination In Preterm Pigs, Line I Christiansen, Gemma C Ventura, Bo Holmqvist, Karoline Aasmul-Olsen, Sandy E H Lindholm, Matthew D Lycas, Yuki Mori, Jan Bojsen-Møller Secher, Douglas G Burrin, Thomas Thymann, Per T Sangild, Stanislava Pankratova

Faculty, Staff and Students Publications

INTRODUCTION: Preterm infants have increased risk of impaired neurodevelopment to which reduced systemic levels of insulin-like growth factor 1 (IGF-1) in the weeks after birth may play a role. Hence, we hypothesized that postnatal IGF-1 supplementation would improve brain development in preterm pigs, used as a model for preterm infants.

METHODS: Preterm pigs delivered by cesarean section received recombinant human IGF-1/IGF binding protein-3 complex (rhIGF-1/rhIGFBP-3, 2.25 mg/kg/day) or vehicle from birth to postnatal day 19. Motor function and cognition were assessed by monitoring of in-cage and open field activities, balance beam test, gait parameters, novel object recognition and operant conditioning …

Toward More Rigorous And Informative Nutritional Epidemiology: The Rational Space Between Dismissal And Defense Of The Status Quo, Andrew W Brown, Stella Aslibekyan, Dennis Bier, Rafael Ferreira Da Silva, Adam Hoover, David M Klurfeld, Eric Loken, Evan Mayo-Wilson, Nir Menachemi, Greg Pavela, Patrick D Quinn, Dale Schoeller, Carmen Tekwe, Danny Valdez, Colby J Vorland, Leah D Whigham, David B Allison

Faculty, Staff and Students Publications

To date, nutritional epidemiology has relied heavily on relatively weak methods including simple observational designs and substandard measurements. Despite low internal validity and other sources of bias, claims of causality are made commonly in this literature. Nutritional epidemiology investigations can be improved through greater scientific rigor and adherence to scientific reporting commensurate with research methods used. Some commentators advocate jettisoning nutritional epidemiology entirely, perhaps believing improvements are impossible. Still others support only normative refinements. But neither abolition nor minor tweaks are appropriate. Nutritional epidemiology, in its present state, offers utility, yet also needs marked, reformational renovation. Changing the status quo …

Australian Highlevel Public Policy Preparedness For Population-Based Triage During The Pandemic, Zachary Horn, Lily Gapp Duckett, Kaitlin Webber

Research outputs 2022 to 2026

The COVID-19 pandemic brought attention to scarce clinical resource allocation via secondary population-based triage (S-PBT) throughout the international healthcare community. Experiences overseas highlighted the importance of coordinated and consistent approaches to allocating resources when facing overwhelming demand, particularly for critical care. Noting the importance of consistency and the system of devolved governance deployed in Australia, this study aimed to identify and analyse sources of high-level policy that affect Australia’s health system preparedness for the operationalisation of S-PBT. Of the 39 documents reviewed, 17 contained potential references to S-PBT. There was a lack of clear recommendations and guidance to inform S-PBT …

Development Of A Tool To Identify Barriers And Enablers To Practice Innovation In Midwifery: A Participatory Action Research Study, Sara D. Davis, Sara Bayes, Sadie Geraghty

Research outputs 2022 to 2026

Introduction: Transferring research evidence into midwifery practice is fraught with challenges and obstacles. Implementation tools can streamline the process and are most effective when they are discipline-specific; however, there are currently no midwifery specific implementation tools. The aim of this study was to develop a midwifery specific tool to identify barriers and enablers to evidence-informed practice change within the clinical setting. Methods: Participatory action research methodology was employed to ensure potential end-users contributed to content and format of the tool. Purposeful sampling ensured participants were selected from a range of midwifery practice settings in Western Australia and the United Kingdom. …

Discovery Of A Missense Mutation (Q222k) Of The Apoe Gene From The Australian Imaging, Biomarker And Lifestyle Study, Blaine R. Roberts, Scott B. Laffoon, Anne M. Roberts, Tenielle Porter, Chris Fowler, Colin L. Masters, Edward A. Dratz, Simon M. Laws

Research outputs 2022 to 2026

After age, polymorphisms of the Apolipoprotein E (APOE) gene are the biggest risk factor for the development of Alzheimer's disease (AD). During our investigation to discovery biomarkers in plasma, using 2D gel electrophoresis, we found an individual with and unusual apoE isoelectric point compared to APOE ϵ2, ϵ3, and ϵ4 carriers. Whole exome sequencing of APOE from the donor confirmed a single nucleotide polymorphism (SNP) in exon 4, translating to a rare Q222K missense mutation. The apoE ϵ4 (Q222K) mutation did not form dimers or complexes observed for apoE ϵ2 ϵ3 proteins.

Impact Of Particle Flux On The Vertical Distribution And Diversity Of Size-Fractionated Prokaryotic Communities In Two East Antarctic Polynyas, Viena Puigcorbé, Clara Ruiz-González, Pere Masqué, Josep M. Gasol

Research outputs 2022 to 2026

Antarctic polynyas are highly productive open water areas surrounded by ice where extensive phytoplankton blooms occur, but little is known about how these surface blooms influence carbon fluxes and prokaryotic communities from deeper waters. By sequencing the 16S rRNA gene, we explored the vertical connectivity of the prokaryotic assemblages associated with particles of three different sizes in two polynyas with different surface productivity, and we linked it to the magnitude of the particle export fluxes measured using thorium-234 (²³⁴Th) as particle tracer. Between the sunlit and the mesopelagic layers (700 m depth), we observed compositional changes in the …

Muscle Strength And Activity In Men And Women Performing Maximal Effort Biceps Curl Exercise On A New Machine That Automates Eccentric Overload And Drop Setting, James L. Nuzzo, Matheus D. Pinto, Kazunori Nosaka

Research outputs 2022 to 2026

Purpose:

Connected adaptive resistance exercise (CARE) machines are new equipment purported to adjust resistances within and between repetitions to make eccentric (ECC) overload and drop sets more feasible. Here, we examined muscle strength, endurance, electromyographic activity (EMG), and perceptions of fatigue during unilateral bicep curl exercise with a CARE machine and dumbbells. We also tested for sex differences in muscle fatigability.

Methods:

Twelve men and nine women attempted 25 consecutive coupled maximal ECC – concentric (CON) repetitions (ECCmax – CONmax) on a CARE machine. Participants also completed a CON one repetition maximum (1RM) and repetitions-to-failure tests with 60 and 80 …

The Effect Of Regular Consumption Of Reformulated Breads On Glycemic Control: A Systematic Review And Meta-Analysis Of Randomized Clinical Trials, Alena M. Schadow, Ingrid Revheim, Ulrike Spielau, Jutta Dierkes, Lukas Schwingshackl, Jan Frank, Jonathan M. Hodgson, André Moreira-Rosário, Chris J. Seal, Anette E. Buyken, Hanne Rosendahl-Riise

Research outputs 2022 to 2026

Bread is a major source of grain-derived carbohydrates worldwide. High intakes of refined grains, low in dietary fiber and high in glycemic index, are linked with increased risk for type 2 diabetes mellitus (T2DM) and other chronic diseases. Hence, improvements in the composition of bread could influence population health. This systematic review evaluated the effect of regular consumption of reformulated breads on glycemic control among healthy adults, adults at cardiometabolic risk or with manifest T2DM. A literature search was performed using MEDLINE, Embase, Web of Science and the Cochrane Central Register of Controlled Trials. Eligible studies employed a bread intervention …

Transition Of Transnational Social Workers: A Critical Realist Perspective On The Need For A Response From The Profession, Shajimon Peter, Allen Bartley, Liz Beddoe, Lynne Soon-Chean Park, Liyun Wendy Choo

Research outputs 2022 to 2026

This article examines the absence of a profession-wide response for facilitating the transition of transnational social workers (TSWs) into the host country. TSWs work with various racial, ethnic, and cultural groups and often in unfamiliar socio-political, policy and practice contexts. A qualitative study was undertaken in New Zealand, which involved six focus groups, three with managers of TSWs and three with TSWs themselves and a face-to-face semi-structured interview with a manager who was also a TSW. Coding and analysis of data were informed by critical realism (CR). We found that TSWs’ transitions are framed within an employer-employee relationship in the …

Vitamin K1 Intake And Incident Diabetes In The Danish Diet Cancer And Health Study: Supplemental Information, Pratik Pokharel, Jamie W. Bellinge, Frederik Dalgaard, Kevin Murray, Marc Sim, Bu B. Yeap, Emma Connolly, Lauren C. Blekkenhorst, Catherine P. Bondonno, Joshua Lewis, Gunnar Gislason, Anne Tjønneland, Kim Overvad, Jonathan M. Hodgson, Carl Schulz, Nicola P. Bondonno

Research outputs 2022 to 2026

No abstract provided.

The Role Of Inclusive Leadership In Fostering Organisational Learning Behaviour, Mehran Nejati, Azadeh Shafaei

Research outputs 2022 to 2026

Purpose: Organisational learning is fundamental in establishing a fearless organisation, creating a competitive advantage and maintaining a sustained growth. While research suggests that leaders can influence organisational learning, there is currently no empirical evidence on how inclusive leadership fosters organisational learning behaviour. Therefore, the purpose of this study is to investigate the relationship between inclusive leadership and organisational learning behaviour. It also seeks to explore the mediating role of psychological safety and climate for initiatives in the mentioned relationship. Design/methodology/approach: The study used a two-wave quantitative examination with 317 respondents. Online survey was used to collect data from randomly selected …

Clinical Practice Guidelines On Hemochromatosis: Asian Pacific Association For The Study Of The Liver, Darrell H. G. Crawford, Grant A. Ramm, Kim R. Bridle, Amanda J. Nicoll, Martin B. Delatycki, John K. Olynyk

Research outputs 2022 to 2026

Hereditary hemochromatosis is the result of pathogenic variants in multiple genes that can result in increased body iron stores with excess iron deposited in various organs, including the liver, pancreas, and heart. The two most important advances in the field over the past 30 years have been the identification of the HFE gene (and the associated p.Cys282Tyr substitution), and the discovery of the hormone hepcidin, which is inappropriately low in this condition and is the pathophysiological basis of the increased iron absorption. The identification of mutations in the HFE gene and subsequent studies have reshaped diagnostic algorithms resulting in a …

Customer-Based Brand Equity And Customer Behavioral Intention: Evidence From Insurance Service, Elaheh Bakhshizadeh, Hossein Aliasghari

Research outputs 2022 to 2026

Objectives: Brands as effective tools and the most valuable intangible assets of companies are widely applied to change customer behavioral intentions. Creating brands with high positions in customers' minds which can offer great value to them and affect their decision-making processes and purchase intentions is companies' priority. This study examines the effects of customer-based brand equity and its dimensions comprising brand awareness, brand association, perceived quality, and brand loyalty (Aaker's model) on customer repurchase intention through customer perceived value in the light of signalling theory. Method: These relationships are investigated on a sample of Iranian life insureds (267 customers) in …

Editorial: Viral Infection, Tumor Development And Host Immune Response, Xin Li, Liwei An, Yucui Jin, Jun Gui, Weitao Lin, Zhenke Wen, Muzaffer Ahmad Kassab, Jie Chen

Research outputs 2022 to 2026

Cancer is a devastating disease that remains a leading cause of death worldwide. According to recent statistics, cancer was responsible for 10 million deaths in 2020 alone (Ferlay et al., 2021). Of these cases, viral infections associated cancers have been found to contribute to a significant proportion, with chronic infections such as hepatitis B and C viruses (HBV and HCV), human papillomavirus (HPV), Epstein-Barr virus (EBV), and Human immunodeficiency virus (HIV) being particularly noteworthy. Inflammation and immune response are also closely linked to tumorigenesis, making it crucial to understand how viral infections and host immune response contribute to cancer development …

Microvascular And Macrovascular Complications Of Type 2 Diabetes Mellitus: Exome Wide Association Analyses, Afnan Mansour, Mira Mousa, Dima Abdelmannan, Guan Tay, Ahmed Hassoun, Habiba Alsafar

Research outputs 2022 to 2026

Background: Type 2 diabetes mellitus (T2DM) is a chronic, metabolic disorder in which concomitant insulin resistance and β-cell impairment lead to hyperglycemia, influenced by genetic and environmental factors. T2DM is associated with long-term complications that have contributed to the burden of morbidity and mortality worldwide. The objective of this manuscript is to conduct an Exome-Wide Association Study (EWAS) on T2DM Emirati individuals to improve our understanding on diabetes-related complications to improve early diagnostic methods and treatment strategies. Methods: This cross-sectional study recruited 310 Emirati participants that were stratified according to their medically diagnosed diabetes-related complications: diabetic retinopathy, diabetic neuropathy, diabetic …

Perceived Nexus Between Non-Invigilated Summative Assessment And Mental Health Difficulties: A Cross Sectional Studies, Amanda Graf, Esther Adama, Ebenezer Afrifa-Yamoah, Kwadwo Adusei-Asante

Research outputs 2022 to 2026

The COVID-19 pandemic rapidly led to changes in the mode of teaching, learning and assessments in most tertiary institutions worldwide. Notably, non-invigilated summative assessments became predominant. These changes heightened anxiety and depression, especially among individuals with less resilient coping mechanism. We explored the perceptions and experiences of mental health difficulties of students in tertiary education regarding non-invigilated alternative assessments in comparison to invigilated assessments. A pragmatic, mixed method cross sectional design was conducted online via Qualtrics. Thematic analysis of text was carried out using NVivo 12. In the quantitative analysis, univariable and multivariable ordinal logistic models were used to examine …

Social Media And English Language Writing Performances Of A Ghanaian Esl Class: The Nexus, Ramos Asafo-Adjei, Ronald Osei Mensah, Ernest Kwesi Klu, Enock Swanzy-Impraim

Research outputs 2022 to 2026

The nexus among social media usage and the English language writing performances of a Ghanaian English as Second Language (ESL) class was investigated in this enquiry. The simple qualitative case study design was used, and the data was collected from a co-educational government secondary school Form 2 General Arts One class in Ghana. A focus group discussion (FGD) guide was employed to elicit the data, and the data were synthesised and analysed using the Data Analysis Spiral. It was evidenced that social media has adversely impacted the students’ English Language learning, as oblivious and non-standard contents have been smuggled into …

Philippine Gabaldon Heritage School Buildings In The New Normal : Safety, Sustainability And Human Development Perspectives, Inero Ancho

Journal of Urban Culture Research

As the COVID-19 pandemic has impacted education, stakeholders find means to return to the New Normal through limited face-to-face learning. The Philippine Gabaldon School Buildings (GSBs) are seen as venues where this can happen. Built from 1908 –1915, GSBs were erected during the American colonial government. Republic Act 11194 mandates these buildings’ conservation. Due to its perceived astounding structure and cultural value, a qualitative study was conducted that delved into the existing publicly available documents related to the buildings, for in-depth analysis. The investigation highlights how GSBs potentially address the minimum health standards relevant to the pandemic and limited face-to-face …

Rasulan As A Javanese Slametan Tradition On Compromising The Changing Of Society, Sartini Sartini, Supartiningsih Supartiningsih, Sriyulita Pramulia Panani, Adita Nurdia Damayanti, Anwar Ibrahim Triyoga

Journal of Urban Culture Research

Rasulan is a tradition in Java; it's one form of Javanese Slametan (a ritual meal). This research aims to describe the Rasulan, explains the role of the worldview and how the elements of culture, religion and the spirit of the nationality are compromised and changed therein. This research is a mix of library and field studies, utilizing the theory of worldview. Results show that Rasulan is not a religious tradition, but a harvest celebration, taking the form of gratitude in the agrarian society. It consists of world views of space, time, causality and self-other relationships, which constructs how people …

“Hin Le Le” Folk Songs In Phitsanulok Province : Identity And Existence In Times Of The Covid-19 Pandemic, Sasinut Phongnil

Journal of Urban Culture Research

The objectives of this qualitative research were to investigate (1) the identity of folk songs in Phitsanulok province, Thailand (2) the forms, methods and existence of folk songs during the COVID-19 pandemic, using musicological methodology. The examination of the background, musical characteristics, components and forms at present revealed that the identity of Hin Le Le folk songs is “the songs that reflect the way of life of the locals in Phitsanulok province, keep up with ongoing events for all age groups and are created by the elderly in the community.” The examination of the forms and existence methods of folk …

Identifying The Key Value Of Urban Architectural Heritages Vis-A-Vis Human Awareness : Case Study In Hanoi, Nhu Dao Thi

Journal of Urban Culture Research

Conservation of urban architectural heritage works (UAH) is an important test for cities to increase their cultural and economic competitiveness. However, UAH preservation mostly focuses on physical value, which results in a contradiction between conservation and development. Moreover, new contexts of 4.0 technology and urbanization are deepening the impermanence, transformation of all things, in which of people and their awareness - the beneficiaries of UAHs. Therefore, heritage conservation is facing a big problem related to the truly worthy values of UAH that need to be preserved to meet the development needs, society's change and the growing maturity of people's awareness. …