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Frontmatter (Volume 44, Issue 4), Paul B. Mojzes May 2024

Frontmatter (Volume 44, Issue 4), Paul B. Mojzes

Occasional Papers on Religion in Eastern Europe

No abstract provided.


Does Contingent Reward Leadership Enhance Or Diminish Team Creativity? It Depends On Leader (Un-) Predictability, Debjani Ghosh, Martin Buss, Amita Shivhare May 2024

Does Contingent Reward Leadership Enhance Or Diminish Team Creativity? It Depends On Leader (Un-) Predictability, Debjani Ghosh, Martin Buss, Amita Shivhare

Management Faculty Publications

Although prior research has shown that reward provision might sometimes increase creativity, little is known about how leadership that clarifies effort-reward contingencies (i.e., contingent reward leadership) is related to team creativity. Drawing on the theory of learned industriousness, we argue that contingent reward leadership can enhance team knowledge exchange and, in turn, team creative performance. However, we propose that this relationship is moderated by leader unpredictability, which can create uncertainty about resource allocation, thereby undermining the otherwise positive effect of contingent reward leadership. In a two-source, lagged design (three-wave) field study with data from 60 organizational teams, we found a …


Robo-Advisers And Investor Behavior, Benjamin Loos, Pengfei Ma, Alessandro Previtero, Andres Hackethal May 2024

Robo-Advisers And Investor Behavior, Benjamin Loos, Pengfei Ma, Alessandro Previtero, Andres Hackethal

Sim Kee Boon Institute for Financial Economics

Using data from a large German retail bank, we investigate the effects of the introduction of a robo-advisory tool on clients’ portfolios. Robo clients experience a significant increase in financial risk-taking and improve portfolio efficiency along several dimensions (more diversification, less home bias, more investment in passive funds, reduced investment fees). Our evidence is consistent with robo-advisers being a more cost-effective approach for offering financial advice, especially to less sophisticated investors.


Meta-Analytic Investigations Of Team Development Interventions And Effectiveness, Sydney Begerowski May 2024

Meta-Analytic Investigations Of Team Development Interventions And Effectiveness, Sydney Begerowski

All Dissertations

Team development interventions (TDIs) are evidenced-based best practices that improve performance trajectories of teams. To date, various independent studies have demonstrated the effectiveness of one type of TDI in improving effectiveness, but there has yet to be a cross-intervention assessment. Accordingly, the goal of this dissertation was to explore a subset of TDIs (team charters, team training, team debriefing) from a comparative lens. First, I conducted a series of pairwise meta-analyses across team charters, team training, and team debriefing to establish a TDI-effectiveness relationship. Findings were inconclusive regarding team charters, but suggested that team training and team debriefing significantly improve …


Phylogenetics And Biogeography Of Appalachian Trechus (Coleoptera: Carabidae), Patricia Wooden May 2024

Phylogenetics And Biogeography Of Appalachian Trechus (Coleoptera: Carabidae), Patricia Wooden

All Dissertations

Insects have long been used as tools for understanding the health of the natural world and how changes to climate, landscape, and human interaction have affected the communities those environments support. The Appalachian Mountains, located in eastern North America, is the oldest mountain range on the continent, and during its lifespan it has supported an abundance of microhabitats and endemic species to fill them. Conservationists must balance the need to preserve the diversity that is known and continue to explore the region for new taxa, often with limited budget and expertise. A genus of small carabids, Trechus Clairville, may aid …


Reinforcement Learning-Based Energy Management For Electric Vehicle Application, Yiming Ye May 2024

Reinforcement Learning-Based Energy Management For Electric Vehicle Application, Yiming Ye

All Dissertations

The increasing concerns about transportation pollution and fossil fuel depletion motivate many studies on vehicle electrification and advanced energy-saving propulsion systems. When Comparing with traditional internal combustion engine vehicles, electrified vehicles, such as battery and supercapacitor electric vehicles, are equipped with more than one power source in the hybrid propulsion system, which can save more energy through efficient power combinations. Lithium-ion batteries are the preferred choice for energy storage in electric vehicles due to their superior energy density and cost-effectiveness. Nevertheless, matching the required power input and output leads to in an unwanted growth in the size of the battery, …


Examining Policy Diffusion Mechanisms In The Contemporary Local Government Landscape Of A Politically Polarized United States, Jacob Mccauley May 2024

Examining Policy Diffusion Mechanisms In The Contemporary Local Government Landscape Of A Politically Polarized United States, Jacob Mccauley

All Dissertations

This dissertation explores the nuanced relationship between political polarization and policy diffusion within local governments in the United States, a topic of considerable relevance given the country’s increasing political divide. By examining the diffusion of COVID-19 policies, historical marker modifications, and the implementation of eGovernment services, this research identifies a novel mechanism of policy diffusion that is significantly influenced by political party lines. The study employs a comprehensive methodological approach, incorporating case studies and quantitative analysis to examine policy implementation across politically diverse local governments. The findings reveal that political affiliation profoundly impacts policy choices, with distinct patterns of policy …


A Rhetorical-Sociological Understanding Of Emotion, Amy Boone May 2024

A Rhetorical-Sociological Understanding Of Emotion, Amy Boone

All Dissertations

This project presents a rhetorical-sociological understanding of emotion while foregrounding the place and role of language. Born from Sara Ahmed’s work in The Cultural Politics of Emotion, it presents a simplified equation (contact + orientation = emotion) as both a prescriptive and descriptive formula for making sense of human emotional experience. Starting with an exploration of the difficult-to-understand affect system, this dissertation identifies both rhetoric and affect as energies which cannot be understood apart from their traces, the things they move, produce, and become. As a way to get at these often-invisible forces, two trace studies are conducted which explore …


Exploring Novel Nutrient Acquisition Mechanisms For Maintaining Energy Metabolism In Toxoplasma Gondii, Melanie Key May 2024

Exploring Novel Nutrient Acquisition Mechanisms For Maintaining Energy Metabolism In Toxoplasma Gondii, Melanie Key

All Dissertations

Toxoplasma gondii is an infectious intracellular parasite that establishes infection within various mammalian hosts, including humans. Toxoplasma infects approximately one-third of the global population, making it an impactful pathogen of study. T. gondii is the causative agent of toxoplasmosis, an opportunistic infection that causes severe illness or fatality in immunocompromised individuals or pregnant women. The parasite possesses a very effective invasion strategy, allowing it to cross different tissue types to disseminate infection across the placental and blood-brain barrier. Although there are a few treatments for acute toxoplasmosis, these drugs can produce adverse side effects. These options are not effective against …


An Exploration Of The Efficacy Of Virtual Agents Within Smartphone Mental Health Applications, Stephanie Six May 2024

An Exploration Of The Efficacy Of Virtual Agents Within Smartphone Mental Health Applications, Stephanie Six

All Dissertations

The prevalence of depression in the U.S. has increased over the past decade, leading to an all-time-high during the COVID-19 pandemic (WHO, 2022). With this increase, the number of mental health applications (MHealth apps) on virtual e-stores increases in tandem. Despite this expanding number of MHealth apps, few demonstrate a foundation in empirical research. One design factor that may influence usability and effectiveness is the inclusion of virtual agents. Three studies were conducted to investigate users’ preferences for a variety of characteristics associated with virtual agents. In Experiment 1, users completed a single-session, three-stage CBT-based interaction with virtual characters and …


Experiences Of Vertical Transfer Students In The Department Of Biological Sciences With The Transfer Credit Process: A Phenomenological Study, Laura Love May 2024

Experiences Of Vertical Transfer Students In The Department Of Biological Sciences With The Transfer Credit Process: A Phenomenological Study, Laura Love

All Dissertations

The purpose of this interpretive phenomenological study was to highlight the experiences of undergraduate STEM vertical transfer students. Through the lens of Schlossberg’s Transition Theory, this study sought to better understand how students make sense of the transfer credit process and which support structures facilitated their understanding. Semi-structured interviews were conducted using qualitative methods with eight biological sciences and vertical transfer students currently enrolled at a large public four-year land-grant research institution in the Southeast United States. All participants had successfully transferred from one of 16 technical colleges within the state. Using interpretative phenomenological analysis, three themes were identified. This …


Design And Application Of Smart Systems To Address Analytical Problems, Lucas B. Ayres May 2024

Design And Application Of Smart Systems To Address Analytical Problems, Lucas B. Ayres

All Dissertations

This dissertation is a multidisciplinary effort that integrates low-cost analytical instrumentation, redox chemistry, and artificial intelligence to overcome existing limitations in the fields of wearable sensing technology, Deep Eutectic Solvents (DES), and antioxidant chemistry. The overall goal behind each implemented strategy is to enhance the accuracy, efficiency, and accessibility of analytical processes and technologies. A general overview of the thesis, along with the research outcomes is included in Chapter One. The theoretical framework of this dissertation is presented in Chapter Two. Chapter Three describes the development of a wearable platform (sensor and instrumentation) to rapidly detect (~20 minutes) S. aureus …


Communication Of Oncology Genomics: Patients, Providers, And Artificial Intelligence, Lisa Stewart May 2024

Communication Of Oncology Genomics: Patients, Providers, And Artificial Intelligence, Lisa Stewart

All Dissertations

ABSTRACT This dissertation comprises five chapters concentrated on the communication of molecular profiling (MP) results between patients with cancer and oncology providers. Chapter I introduces MP and describes the significance of this testing. Chapter II is a state-of-the-art literature review focused on cancer patients’ expectations, knowledge, attitudes, satisfaction, and concerns regarding MP. Nineteen articles were reviewed, and findings revealed gaps in the communication process between patients and clinicians. Chapter III is a case study entitled “A Case for Caution: Patient Use of Artificial Intelligence.” In this case, pitfalls become apparent when a patient utilizes an artificial intelligence (AI) chatbot as …


Evolution And Biogeography Of High Appalachian Rove Beetles (Staphylinidae), Adam Haberski May 2024

Evolution And Biogeography Of High Appalachian Rove Beetles (Staphylinidae), Adam Haberski

All Dissertations

The southern Appalachian Mountains are a region of great species richness and a center of endemism in many taxa, including flightless arthropods. High elevation refugia have facilitated the persistence of ancient lineages (paleoendemism) and in situ speciation (neoendemism), but their ubiquity, timing, and relative importance is unknown. Congruent patterns among co-distributed, but independent taxa might illuminate common drivers of Appalachian endemism. In this dissertation, I investigated the influence of allopatric speciation, geography, and paleoclimate events on the diversification of endemic species in two genera of rove beetles (Coleoptera, Staphylinidae), Lathrobium Gravenhorst and Ischnosoma Stephens, using an integrative morphological and molecular …


Real-Time Degradation Abatement Framework For Energy Storage System In Automotive Application Using Data-Driven Approaches, Laxman Timilsina May 2024

Real-Time Degradation Abatement Framework For Energy Storage System In Automotive Application Using Data-Driven Approaches, Laxman Timilsina

All Dissertations

The increasing popularity of electric vehicles (EVs) is driven by their compatibility with sustainable energy goals. However, the decline in the performance of energy storage systems, such as batteries, due to their degradation puts EVs and hybrid electric vehicles (HEVs) at a disadvantage compared to traditional internal combustion engine (ICE) vehicles. The batteries used in these vehicles have limited life. The degradation of the battery is accelerated by the operating conditions of the vehicle, which further reduces its life and increases the reliability and economic concerns for the vehicle’s operation. The aging mechanism inside a battery cannot be eliminated but …


El Leñador, May 2024, El Leñador Staff May 2024

El Leñador, May 2024, El Leñador Staff

El Leñador Newspaper

El Leñador is an English and Spanish newspaper produced by Humboldt State University students. Our staff cover and provide news to Latinx and other diverse communities on campus and in Humboldt County. We are committed to providing relevant news and expanding the representations and stories told about people of color and other marginalized groups. Our work helps create more social, political and cultural diversity in local media.

El Leñador es un periodico en español y inglés producido por estudiantes de la Universidad Estatal de Humboldt. Nuestro personal cubre y provee noticias a la comunidad Latinx y otras comunidades diversas en …


Assessing Consumer Willingness To Pay For Zinc-Biofortified Rice – The Case Of Colombia, Sara Ann Oswalt May 2024

Assessing Consumer Willingness To Pay For Zinc-Biofortified Rice – The Case Of Colombia, Sara Ann Oswalt

Graduate Theses and Dissertations

Hidden hunger, a form of undernourishment caused by micronutrient deficiencies, remains a critical global challenge affecting billions worldwide. Biofortification of staple crops, such as rice, offers a promising solution to address this problem. However, changes in the physical and culinary characteristics due to biofortification may hinder consumer acceptance and limit the benefits of this approach. This research explores consumer preferences and willingness to pay (WTP) for zinc-biofortified rice in Cartagena, Colombia. A non-hypothetical experimental auction, including 400 consumers in five different locations within Cartagena, was conducted to analyze the impact of information, socioeconomic factors, and consumer nutrition knowledge on WTP …


Cultivating Atmosphere In Independent Coffee Shops: Reflections On How Atmospheres Are Created Through Sensory Experiences, Brooke Bierhaus Sutton May 2024

Cultivating Atmosphere In Independent Coffee Shops: Reflections On How Atmospheres Are Created Through Sensory Experiences, Brooke Bierhaus Sutton

Graduate Theses and Dissertations

Affective atmosphere is a fundamental component to the allure of coffee shops that is cultivated through sensory experience. In this thesis I examine how the sensory experience of sight, sound, smell, taste, and touch, create atmospheres in coffee shops. I ask, “how do coffee shops cultivate an affective atmosphere?” Through an exploration of material culture, design, affect, and the intentional production of third places, I illustrate the ways in which independent coffee shops have their own unique identities and atmospheres. By using memoir and reflexive analysis of my decade in global coffee culture, as well as an extensive literary review, …


The Educator Labor Market In Times Of Change: Lessons From The Covid-19 Pandemic, Andrew Mitchell Camp May 2024

The Educator Labor Market In Times Of Change: Lessons From The Covid-19 Pandemic, Andrew Mitchell Camp

Graduate Theses and Dissertations

The COVID-19 pandemic placed an undeniable burden onto the teaching profession which already faced levels of prestige and interest at 50-year lows. The strains of the pandemic resulted in educators reporting higher levels of job-related stress and burnout and exacerbated concerns about potential teacher shortages. This dissertation leverages administrative data from Arkansas to better understand challenges facing the teacher workforce following COVID-19 and evaluate two increasingly policies aimed at the teacher workforce. In the first chapter, I provide evidence that teacher turnover was relatively stable during the first two years (2020-21 and 2021-22) of the COVID-19 pandemic before a notable …


Global Landscape Of The Attack Of Predatory Journals In Oncology, Khalid El Bairi, Dario Trapani, Sara Nidhamalddin, Shah Khan, Arman Chowdhury, Csongor Lengyel, Sadaqat Hussain, Baker Habeeb, Angelica Petrillo, Miriam Mutebi May 2024

Global Landscape Of The Attack Of Predatory Journals In Oncology, Khalid El Bairi, Dario Trapani, Sara Nidhamalddin, Shah Khan, Arman Chowdhury, Csongor Lengyel, Sadaqat Hussain, Baker Habeeb, Angelica Petrillo, Miriam Mutebi

General Surgery, East Africa

Purpose Open-access publishing expanded opportunities to give visibility to research results but was accompanied by the proliferation of predatory journals (PJos) that offer expedited publishing but potentially compromise the integrity of research and peer review. To our knowledge, to date, there is no comprehensive global study on the impact of PJos in the field of oncology.

Materials and Methods A 29 question-based cross-sectional survey was developed to explore knowledge and practices of predatory publishing and analyzed using descriptive statistics and binary logistic regression.

Results Four hundred and twenty-six complete responses to the survey were reported. Almost half of the responders …


The Banality Of Thinking: The Criticism Of Hannah Arendt’S "Eichmann In Jerusalem", J. Lynne Nelson May 2024

The Banality Of Thinking: The Criticism Of Hannah Arendt’S "Eichmann In Jerusalem", J. Lynne Nelson

History Honors Theses

No abstract provided.


Do Executive Functions Buffer Against Covid-19 Fear And Stress? A Latent Variable Approach, Tina Li Yi Ng, Nadyanna M. Majeed, Verity Yu Qing Lua, Andree Hartanto May 2024

Do Executive Functions Buffer Against Covid-19 Fear And Stress? A Latent Variable Approach, Tina Li Yi Ng, Nadyanna M. Majeed, Verity Yu Qing Lua, Andree Hartanto

Research Collection School of Social Sciences

Levels of COVID-19 stress have soared worldwide as a result of the pandemic. Given the pernicious psychological and physiological effects of stress, there is an urgent need for us to protect populations against the pandemic’s psychological impact. While there exists literature documenting the prevalence of COVID-19 stress among various populations, insufficient research has investigated psychological factors that might mitigate this worrying trend. To address this gap in the literature, the current study seeks to examine executive functions as a potential cognitive buffer against COVID-19 stress. To do so, the study adopted a latent variable approach to examine three latent factors …


Can Confidence Influence Persuasiveness In Disagreements By Conveying Competence Versus Dominance? The Moderating Role Of Competitiveness, Laura Rees, Ming-Hong Tsai, Shirli Kopelman, Hsiu-Hua Hu May 2024

Can Confidence Influence Persuasiveness In Disagreements By Conveying Competence Versus Dominance? The Moderating Role Of Competitiveness, Laura Rees, Ming-Hong Tsai, Shirli Kopelman, Hsiu-Hua Hu

Research Collection School of Social Sciences

Research has demonstrated that confident individuals gain social influence because their confidence signals competence rather than dominance in settings in which they do not experience a disagreement with others. We extend this research by exploring felt competitiveness, as reflected by perceptions of goal opposition between perceivers and others. In settings where people experience a disagreement, we explore the impact of felt competitiveness on the association between expressed confidence and social perceptions of the expresser’s competence and dominance, and how these shape persuasiveness. We conducted a field study examining dyadic interactions between coworkers (Study 1) and two experimentsmanipulating competitiveness and confidence …


Evaluating Mental Health Outcomes In Covid-19 Icu Survivors: A Scoping Review Of Measurement Tools, Kimberly T'Ng, Justin Kenardy, Andree Hartanto May 2024

Evaluating Mental Health Outcomes In Covid-19 Icu Survivors: A Scoping Review Of Measurement Tools, Kimberly T'Ng, Justin Kenardy, Andree Hartanto

Research Collection School of Social Sciences

Objective: The objective of this scoping review was to map the range of measurement tools used to study the prevalence of common mental health conditions in COVID-19 ICU survivors. Introduction: Increased rates of admission to and survivorship from intensive care units (ICUs) have been observed in recent years, particularly during the global pandemic. ICU patients are at a higher risk of developing depressive, anxiety, and PTSD symptoms. Due to the high burden of disease, an accurate understanding of long-term mental health challenges for this population is key. Unfortunately, there is significant variability in reported prevalence rates. Heterogeneity in measurement tools …


Person-Centred Interventions To Improve Patient-Provider Relationships For Hiv Services In Low- And Middle-Income Countries: A Systematic Review, Laura K Beres, Ashley Underwood, Noelle Le Tourneau, Christopher Galloway Kemp, Gauri Kore, Lauren Yaeger, Jingjia Li, Alec Aaron, Claire Keene, Deepthi Priyanka Mallela, Banda A A Khalifa, Aaloke Mody, Sheree Renae Schwartz, Stefan Baral, Chanda Mwamba, Kombatende Sikombe, Ingrid Eshun-Wilson, Elvin H Geng, Marie-Claude C Lavoie May 2024

Person-Centred Interventions To Improve Patient-Provider Relationships For Hiv Services In Low- And Middle-Income Countries: A Systematic Review, Laura K Beres, Ashley Underwood, Noelle Le Tourneau, Christopher Galloway Kemp, Gauri Kore, Lauren Yaeger, Jingjia Li, Alec Aaron, Claire Keene, Deepthi Priyanka Mallela, Banda A A Khalifa, Aaloke Mody, Sheree Renae Schwartz, Stefan Baral, Chanda Mwamba, Kombatende Sikombe, Ingrid Eshun-Wilson, Elvin H Geng, Marie-Claude C Lavoie

2020-Current year OA Pubs

INTRODUCTION: Person-centred care (PCC) has been recognized as a critical element in delivering quality and responsive health services. The patient-provider relationship, conceptualized at the core of PCC in multiple models, remains largely unexamined in HIV care. We conducted a systematic review to better understand the types of PCC interventions implemented to improve patient-provider interactions and how these interventions have improved HIV care continuum outcomes and person-reported outcomes (PROs) among people living with HIV in low- and middle-income countries.

METHODS: We searched databases, conference proceedings and conducted manual targeted searches to identify randomized trials and observational studies published up to January …


Magdm Model Using Single-Valued Neutrosophic Credibility Matrix Energy And Its Decision-Making Application, Jun Ye, Rui Yong, Wanlu Du May 2024

Magdm Model Using Single-Valued Neutrosophic Credibility Matrix Energy And Its Decision-Making Application, Jun Ye, Rui Yong, Wanlu Du

Neutrosophic Systems with Applications

This paper aims to develop a MAGDM model using single-valued neutrosophic credibility matrix (SVNCM) energy in a SVNCM scenario. To do it, first, SVNCM energy and its score function are presented as a conceptual extension of existing single-valued neutrosophic matrix (SVNM) energy. Then, a MAGDM model is developed in terms of SVNCM energy and its score function in a SVNCM scenario and also its decision algorithm is provided to solve MAGDM problems with SVNCMs. Finally, the developed MAGDM model is applied in the school site selection problem as an actual example, then the comparative investigation of the decision results in …


Coarctation Duration And Severity Predict Risk Of Hypertension Precursors In A Preclinical Model And Hypertensive Status Among Patients, Arash Ghorbannia, Hilda Jurkiewicz, Lith Nasif, Abdillahi Ahmed, Jennifer Co-Vu, Mehdi Maadooliat, Ronald K. Woods, John F. Ladisa Jr. May 2024

Coarctation Duration And Severity Predict Risk Of Hypertension Precursors In A Preclinical Model And Hypertensive Status Among Patients, Arash Ghorbannia, Hilda Jurkiewicz, Lith Nasif, Abdillahi Ahmed, Jennifer Co-Vu, Mehdi Maadooliat, Ronald K. Woods, John F. Ladisa Jr.

Mathematical and Statistical Science Faculty Research and Publications

BACKGROUND:

Coarctation of the aorta (CoA) often leads to hypertension posttreatment. Evidence is lacking for the current >20 mm Hg peak-to-peak blood pressure (BP) gradient (BPGpp) guideline, which can cause aortic thickening, stiffening, and dysfunction. This study sought to find the BPGpp severity and duration that avoid persistent dysfunction in a preclinical model and test if predictors translate to hypertension status in patients with CoA.

METHODS:

Rabbits (n=75; 5–12/group) were exposed to mild, intermediate, or severe CoA (≤12, 13–19, ≥20 mm Hg BPGpp) for ≈1, 3, or 22 weeks using dissolvable and permanent sutures with thickening, stiffening, contraction, and endothelial …


Innovation In Targeted Violence And Terrorism Prevention: Phase Ii Summary Report, Joel Elson, Erin M. Kearns May 2024

Innovation In Targeted Violence And Terrorism Prevention: Phase Ii Summary Report, Joel Elson, Erin M. Kearns

Reports, Projects, and Research

This report provides summary findings for data collection efforts in Phase II of the entitled FY21 TVTP grant. In Phase II, the primary aims were to 1) use insights from Phase I to create a chatbot aimed to improve and increase tips reporting and 2) validate the chatbot's efficacy. Importantly, major technological advancement - namely generative chatbots like ChatGPT - occurred between the time this project was proposed in 2021 and data were conducted for this report in 2023. To be responsive to these developments and changing needs associated with them, we developed and tested multiple chatbot systems beyond what …


Government Misinformation Platforms, Janet Freilich May 2024

Government Misinformation Platforms, Janet Freilich

Faculty Scholarship

There is a harmful mismatch between how information published by the government is perceived-as highly trustworthy-and the reality that it is often not. This Article shows that the government frequently collects information from third party private entities and publishes it with no review or vetting. Although this information is riddled with errors and inaccuracies, scholars, policymakers, and the public treat the information with unwarranted confidence because it derives from the government. Further, institutional imprimatur (and consequent trust) attaches to information even tangentially associated with the government and to information where the government explicitly disclaims review.

This Article highlights the ubiquity …


Characterizing Modulators Of Protease-Activated Receptors With A Calcium Mobilization Assay Using A Plate Reader, Jacob T. Derousse, Chris Dockendorff May 2024

Characterizing Modulators Of Protease-Activated Receptors With A Calcium Mobilization Assay Using A Plate Reader, Jacob T. Derousse, Chris Dockendorff

Chemistry Faculty Research and Publications

Changes in calcium concentration in cells are rapidly monitored in a high-throughput fashion with the use of intracellular, fluorescent, calcium-binding dyes and imaging instruments that can measure fluorescent emissions from up to 1,536 wells simultaneously. However, these instruments are much more expensive and can be challenging to maintain relative to widely available plate readers that scan wells individually. Described here is an optimized plate reader assay for use with an endothelial cell line (EA.hy926) to measure the protease-activated receptor (PAR)-driven activation of Gαq signaling and subsequent calcium mobilization using the calcium-binding dye Fluo-4. This assay has been used to …