Digital Commons Network^™

Tsyn-Seq: A T Cell Synapse-Based Antigen Identification Platform, Yimei Jin

Dissertations and Theses (Open Access)

Tools for genome-wide rapid identification of peptide–major histocompatibility complex targets of T-cell receptors (TCRs) are not yet universally available. We present a new antigen screening method, the T-synapse (Tsyn) reporter system, which includes antigen-presenting cells (APCs) with a Fas-inducible NF-κB reporter and T cells with a nuclear factor of activated T cells (NFAT) reporter. To functionally screen for target antigens from a cDNA library, productively interacting T cell–APC aggregates were detected by dual reporter activity and enriched by flow sorting followed by antigen identification quantified by deep sequencing (Tsyn-seq). When applied to a previously characterized TCR specific for the E7 …

Influential Factors And Predicting Dose Delivery Accuracy For Imaging And Radiation Oncology Core’S Phantom Program Using Machine Learning, Hunter Mehrens

Dissertations and Theses (Open Access)

IROC’s mission is to help ensure consistent and comparable, high-quality radiotherapy across clinics that participate in national clinical trials. To obtain this mission, IROC’s phantom program provides a third-party end-to-end check of the clinical workflow of a patient receiving radiotherapy. The goal of the phantom audit is to compare the dose delivered to the dose planned by the treatment system ensuring dose delivery accuracy. While IROC’s phantoms are better equipped to catch dose delivery errors compared to a clinic’s QA process, the end-to-end process and reporting of results is time-consuming creating a bottleneck for clinical trial participation. Furthermore, IROC’s passing …

The Role Of An Ultraconserved Long Non-Coding Rna In B-Cell Lymphomagenesis, Swati Mohapatra

Dissertations and Theses (Open Access)

Ultraconserved regions (UCRs) are genomic segments with perfect (100%) conservation between the orthologous regions of human, rat, and mouse genomes. UCRs can be transcribed into mono-exonic long non-coding RNAs (lncRNAs) known as transcribed ultraconserved regions (T-UCRs). These regions, despite lacking protein-coding potential, are increasingly recognized for their regulatory roles in gene expression, including the modulation of non-coding RNA (ncRNA) transcripts. NcRNAs play crucial roles in cellular processes, including oncogenic transformation, with emerging evidence revealing their ability to encode small peptides known as ncRNA-encoded peptides (ncPEPs). These peptides, originating from small open reading frames (smORFs), contribute to diverse cellular functions and …

The Role Of The Circadian Clock In Disease And Tissue Function, Marvin Wirianto

Dissertations and Theses (Open Access)

The mammalian circadian clock responds to the 24-hour daily cycle and regulates tissue-specific gene expression and downstream physiology. The central pacemaker located in the hypothalamic suprachiasmatic nuclei (SCN) synchronizes the peripheral clocks in different tissues. This dissertation aims to uncover specific circadian regulatory mechanisms in the brain and skeletal muscle.

Alzheimer’s Disease (AD) is the most common type of dementia. It has previously been shown that there is a strong correlation of disease progression with circadian rhythm decline. We showed that circadian modulation by a small molecule Nobiletin (NOB) was able to mitigate AD pathogenesis. Furthermore, NOB significantly alleviated the …

Vesicle-Associated Membrane Proteins In Airway Mucin Secretion, Boxuan Yang

Dissertations and Theses (Open Access)

Mucus plays a vital role in shielding the lungs against harmful substances such as particles, pathogens, and chemicals inhaled from the environment. It captures these harmful elements, and ciliary action helps to expel them from the lungs. Paradoxically, malfunctioning of mucus induces the development of various respiratory illnesses, including asthma, cystic fibrosis, chronic obstructive pulmonary disease (COPD), and interstitial lung diseases. Despite extensive research on controlling mucin production and hydration, the process of secretion is not yet fully comprehended.

Mucin undergoes regulated secretion at two rates: a low baseline rate and a high stimulated rate. Over the past 25 years, …

Recommendations And Implementation Considerations For The Routine Collection Of Sexual Orientation And Gender Identity Data In Research And Practice, Irene Tamí-Maury, Thomas J Millett

Faculty, Staff and Student Publications

No abstract provided.

Plasma Proteins Associated With Plant-Based Diets: Results From The Atherosclerosis Risk In Communities (Aric) Study And Framingham Heart Study (Fhs), Hyunju Kim, Jingsha Chen, Brenton Prescott, Maura E Walker, Morgan E Grams, Bing Yu, Ramachandran S Vasan, James S Floyd, Nona Sotoodehnia, Nicholas L Smith, Dan E Arking, Josef Coresh, Casey M Rebholz

Faculty, Staff and Student Publications

Background & aims: Plant-based diets are associated with a lower risk of chronic diseases. Large-scale proteomics can identify objective biomarkers of plant-based diets, and improve our understanding of the pathways that link plant-based diets to health outcomes. This study investigated the plasma proteome of four different plant-based diets [overall plant-based diet (PDI), provegetarian diet, healthful plant-based diet (hPDI), and unhealthful plant-based diet (uPDI)] in the Atherosclerosis Risk in Communities (ARIC) Study and replicated the findings in the Framingham Heart Study (FHS) Offspring cohort.

Methods: ARIC Study participants at visit 3 (1993-1995) with completed food frequency questionnaire (FFQ) data and proteomics …

Past-Year Hiv Testing, Current Antiretroviral Therapy Use, And Participation In Services For People Who Inject Drugs, Itunu Sokale, Johnny Wilkerson, Paige Wermuth, Folefac Atem, Janet Burnett, Cyprian Wejnert, Salma Khuwaja, Catherine Troisi

Faculty, Staff and Student Publications

Evaluating routine HIV testing and treatment and use of services for people who inject drugs (PWID) is critical to curb the ongoing HIV epidemic. We analyzed data from the 2018 National HIV Behavioral Surveillance of PWID aged 18 years or older, recruited using respondent-driven sampling and offered anonymous HIV testing after survey. We performed bivariate and multivariable analyses with log-linked Poisson regression of the generalized linear models to examine the associations between demographics and PWID service use, past-year HIV testing, and current antiretroviral therapy (ART) use. Among 10,311 HIV-negative PWID, 56% reported past-year HIV testing, and of the 553 HIV-positive …

Analyzing Heterogeneity In Biomarker Discriminative Performance Through Partial Time-Dependent Receiver Operating Characteristic Curve Modeling, Xinyang Jiang, Wen Li, Kang Wang, Ruosha Li, Jing Ning

Faculty, Staff and Student Publications

This study investigates the heterogeneity of a biomarker's discriminative performance for predicting subsequent time-to-event outcomes across different patient subgroups. While the area under the curve (AUC) for the time-dependent receiver operating characteristic curve is commonly used to assess biomarker performance, the partial time-dependent AUC (PAUC) provides insights that are often more pertinent for population screening and diagnostic testing. To achieve this objective, we propose a regression model tailored for PAUC and develop two distinct estimation procedures for discrete and continuous covariates, employing a pseudo-partial likelihood method. Simulation studies are conducted to assess the performance of these procedures across various scenarios. …

E-Cigarette Use Reasons And Associated E-Cigarette Use Dependence Among College Students: A Longitudinal Examination, Tzung-Shiang Ou, Anne Buu, James J Yang, Hsien-Chang Lin

Faculty, Staff and Student Publications

Objective: Previous studies have established an understanding of reasons for e-cigarette use and associated e-cigarette use patterns such as use frequency, yet the critical extension to associated e-cigarette dependence outcome remains under-researched. This study used longitudinal data to examine whether the reasons for e-cigarette use predict a higher/lower level of e-cigarette dependence.

Methods: This study recruited college students who were current e-cigarette users from Fall 2019 to Fall 2020 (four semesters) at three public universities in the Midwest and South of the U.S. Those who participated for at least two semesters were included (N = 366). Data were collected using …

In Vivo Examination Of Peripheral Drivers Of Alzheimer’S Disease, Celso Catumbela

Dissertations and Theses (Open Access)

Alzheimer’s disease (AD) is the leading cause of dementia worldwide, and predominantly affects elderly populations. This disease is well known for its effects on the brain, but a wealth of clinical reports show that dementia can also modify, or be modified by, various peripheral and systemic processes. Yet, the pathological contribution of peripheral comorbidities to AD remains to be fully understood. In an attempt to address some knowledge gaps, we characterized the cerebral and peripheral pathology in mice with history of either liver injury or sepsis. In the former subjects, we found that even in the absence of genetic risk …

The Effect Of Context Diversity In Virtual Reality Environments On Speech Motor Learning In A Person With Aphasia And Apraxia Of Speech, Shelby Keeler Holloway

Theses and Dissertations

This study explored the effect of contextual diversity on speech motor learning in an individual with apraxia of speech (AOS) using virtual reality (VR). One 62-year-old participant with severe AOS participated in the study where he practiced monosyllabic CVC words in both consistent and diverse VR environments. Kinematic, acoustic, and perceptual data were collected and evaluated for evidence of learning. Kinematic data were collected using electromagnetic articulography where sensors were glued to the participant's upper lip, lower lip, tongue blade, and tongue tip and were tracked with high precision. In addition, acoustic and perceptual data were collected using a microphone …

How Fun Overcame Fear: The Gamification Of A Graduate-Level Statistics Course, Mai P. Trinh, Robert J. Chico, Rachel M. Reed

Management Faculty Publications

Innovative instructional methods can help improve student engagement and learning outcomes when teaching difficult subjects, such as statistics. This instructional innovation article illustrates how gamification can be applied in management education to improve students’ learning experience, engagement, and acquisition of knowledge. Our purpose is to demonstrate how gamification is not only a powerful way to build on the use of games and game thinking in our field, but also a versatile application of education technology that could potentially enhance the way management knowledge is taught. Furthermore, it is a low-risk way for management educators to join and contribute to the …

The Effect Of Employee-Oriented Hrm On Employee Downsizing Following Mergers & Acquisitions, Pingshu Li, Mengwei Li, Hyesook Chung, Clint Chadwick

Management Faculty Publications

Firms have increasingly engaged in Mergers and Acquisitions (M&A) activities in recent years. A common strategy following M&A is employee downsizing that aims at achieving strategic goals and enhancing performance. This approach, however, raises concerns from a strategic human resource management (HRM) perspective. Research suggests that downsizing may breach employees' psychological contracts and hinder the development of firm-specific human capital. While this viewpoint has an intuitive appeal, we reconsider it by suggesting that, in the M&A context wherein employees expect significant organizational changes, downsizing does not necessarily lead employees to negative reactions. Instead, if acquiring firms have strong employee-oriented HRM …

Large-Scale Hpc-Empowered Power Electronics Modeling And Simulation In Photovoltaic Applications, Liwei Wang

All Dissertations

The rising popularity of renewable energy sources requires advanced, efficient power electronic systems for energy conversion, grid integration, and system management, thereby raising expectations for power electronics in the energy industry. The complexity of modern power electronic systems requires comprehensive simulations and in-depth analysis to predict performance accurately, but this process is impeded by prolonged simulation times. The primary objective of this dissertation is to develop a high-fidelity, high-speed event-driven simulator to tackle challenges related to mass data processing, uncertainty evaluation, as well as modeling and simulation issues in assessing the reliability of power electronics in large-scale Photovoltaic (PV) systems. …

Voices From The Countryside: Unveiling The Stories Of Rural Teachers In Stem Education, Devan A. Jones

All Dissertations

This narrative study delves into the overlooked perspective of rural communities within the broader educational landscape, highlighting their unique and vital role in education. It primarily aims to gain insights from K-8 teachers working in multigrade, multi-curricular Sustained Deep Rurality (SDR) schools situated in the challenging terrain of the Northern Rocky Mountain area. Employing a methodology centered around episodic narrative interviews and small story analysis, the study uncovers the intricacies and challenges inherent in teaching under the extreme conditions of rural environments.

These SDR schools, situated in remote and isolated regions, serve as vital pillars in rural education, embodying qualities …

Ultra High-Performance Concrete As A High Friction Surface Treatment For Pavements And Bridges, Kyle Maeger

All Dissertations

High Friction Surface Treatment (HFST) is a proven technology to reduce crashes on roadways by increasing the coefficient of friction between vehicle tires and the pavement. However, it is an expensive solution due to the high cost of the two commonly used constituent materials, calcined bauxite aggregate and epoxy resin binders.

This study evaluated the abrasion resistance performance of four alternative HFST aggregates to calcined bauxite using Los Angeles Abrasion and Micro-Deval Abrasion tests, and studied the surface texture utilizing a high-resolution laser scanner and friction utilizing the British Pendulum Test.

This study also assessed the performance of non-proprietary Ultra …

Investigating The Etiology, Epidemiology, And Ecology Of Blackberry Yellow Vein Disease In South Carolina, Wanita Dantes

All Dissertations

Blackberry production is growing in popularity in the southeastern U.S. However, blackberry growers in this region report major problems with productivity and longevity due to viruses. First, the state of knowledge on blackberry yellow vein disease (BVYD) complex, associated with mixed infections of a diverse group of viruses, is discussed. Specific viruses associated with symptom types are unknown. Therefore, the etiology of BYVD is not fully understood, which makes BYVD diagnosis challenging. My research focused on improving BYVD diagnosis, etiology, and epidemiology. Second, I developed a farm-level diagnostic protocol to improve virus disease surveillance. High throughput sequencing detected 17 known …

Parent Advocacy For Literacy Intervention: A Case Study Of Motivations And Experiences, Meghan Malloy

All Dissertations

While systems of support are used in schools to assist students’ academic growth, parents are not always informed of the support process or the academic goals set for their child (RTI Action Network, n.d.; Troisi, 2014; Weingarten et al., 2020). In the aftermath of the pandemic, students present a wide variety of academic needs (Lewis et al., 2021; Maldonado & De Witte, 2020), underscoring the importance of parent advocacy. In situations where a student needs additional supports, such as literacy intervention, the parent may become the child’s best advocate (Besnoy et al., 2015).

Using a multiple case study design, the …

Fundamental Study Of Nonlinear Electrophoresis, Joseph Armakan Bentor

All Dissertations

Classical electrophoretic theory describes the linear response of particles suspended in Newtonian fluids under weak electric fields. When the electric field exceeds this weak limit, a nonlinear response arises due to non-uniform surface conduction over the curved particles. This nonlinear behavior introduces dependencies on fluid and particle properties not considered in the classical theory, offering potential advantages for microfluidic particle manipulation. While this phenomenon has been extensively studied theoretically and numerically in Newtonian fluids, experimental investigations have been relatively limited.

Fluid rheology can significantly alter particle dynamics under applied electric fields. Rheological properties like shear-thinning and elasticity which are not …

Effect Of Long-Term Use Of Antimicrobials On Commonly Used Surfaces In Foodservice Establishments, Anuradhi Bandaranayake

All Dissertations

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Brought What I Needed To Succeed: Community Cultural Wealth In The Persistence Experiences Of Former Division Ii Black Women Basketball Players Who Graduated From Predominantly White Institutions, Tomiko T. Smalls

All Dissertations

Limited research exists to examine the persistence experiences of Black women student-athletes who graduated from predominantly White institutions and to explore their experiences at institutions with Division II membership. This purpose of this study was to examine factors related to the positive graduation outcomes of Black women student-athletes who graduated from predominantly White institutions. The research question was: How did former Division II Black women basketball players engage community cultural wealth to persist to graduation at predominantly White institutions? Five former Division II basketball players who graduated from predominantly White institutions located in the southeastern region of the United Stated …

Base Case Shear-Wave Velocity Profiles And Non-Linear Material Property Models Of Local Site Conditions In South Carolina For Seismic Site Response Analyses, Ali Sedaghat Shirehjini

All Dissertations

This dissertation presents the development of base case small-strain shear-wave velocity ( ) profiles and non-linear materials property models of local site conditions in South Carolina for seismic site response analyses. The need to address South Carolina's seismic hazards is partly motivated by the 1886 Charleston earthquake (moment magnitude, M_w= 6.7 to 7.5). Required inputs for seismic site response analysis include profile down to a specified reference outcrop condition and non-linear material properties for each layer in the profile. Although firm rock (i.e., ≥ 2,500 ft/s) or hard rock (i.e., ≥ 9,850 ft/s) is commonly assumed for the …

No Longer Unseen Or Unheard: Exploring The Connectedness Of Campus Climate With Multiracial Students’ Experiences At Historically White Institutions In The United States, Christian Barrientos

All Dissertations

This qualitative research study will illustrate the link(s) between Multiracial students and their perceptions of campus climate at historically White institutions and how that impacts their sense of belonging. Existing bodies of literature and research have focused more extensively on monoracial students of color than Multiracial students. As a result, there is a formidable gap in the literature about how Multiracial students navigate their racial identity at historically White institutions and how multiracial students perceive campus climate based on their experienced sense of belonging. The use of Kellogg and Liddell (2012) will serve as a guide for this research study. …

Preparing For Kindergarten: An In-Depth Look At The Lived Experiences Of Parents Preparing A Child For Kindergarten, Grace Bache-Wiig

All Dissertations

The purpose of this multi-modal phenomenological study was to examine the lived experiences of parents preparing their first child for kindergarten and to evaluate the sources of information used to inform these experiences. Eight parents of rising kindergarteners were sampled for interviewing from preschool and homeschool settings in North Carolina. Semi-structured interviews were conducted to explore participant experiences and participants identified sources of information to be gathered for content analysis. The interview transcripts were analyzed using van Manen’s approach to identify units of meaning. Four major themes emerged from this study: emotions about kindergarten readiness, an emphasis on social and …

An Emergency Response System To Assist The Movement Of Vehicles During Emergency Operations In Urban Transportation Networks, Jamal Nahofti Kohneh

All Dissertations

Emergency responders need to arrive at the emergency scene as soon as possible, but operating vehicles under emergency conditions can pose a risk to both the responders and other road users, potentially resulting in crashes or delays in emergency operations. In this research, an emergency response system is proposed to assist emergency and non-emergency response vehicles (ERVs and non-ERVs) during emergency operations in a connected vehicle environment. This system collects the information from connected ERVs and non-ERVs, utilizes this information as inputs in the proposed models, and sends instruction messages back to vehicles. The proposed models provide the fastest ERV …

Gan With Skip Patch Discriminator For Biological Electron Microscopy Image Generation, Nishith Ranjon Roy

Graduate Theses and Dissertations

GAN models have been successfully used for image generation in various sections such as real-life objects like human faces, cars, animal faces, landscapes, etc. This work focuses on biological electron microscopy (EM) image generation. Unlike other real-life objects, biological EM images are obtained through electron microscopy techniques to study biological specimens. Electron microscopy offers high resolution and magnification capabilities, making it a powerful tool for visualizing biological structures at the nanoscale. However, using GAN models for biological EM image generation poses challenges due to the complex and unique arrangements of biological structures and the sparse and asymmetrical patterns in EM …

Feasibility Assessment And Container Traffic Forecasting Of Inland Waterway Container On Barge Transportation, Fan Bu

Graduate Theses and Dissertations

Container on Barge (COB) transportation is an intermodal freight transport mode that moves shipping containers via barges on navigable inland and intracoastal waterways. During the past twenty years, COB has been a growing mode of container shipping globally due to its low-cost, eco-friendly, and congestion-reducing characteristics. Europe and China are currently leading global COB transportation, and the United States (U.S.) may have the potential to achieve economic benefits through the implementation of COB within its intermodal transportation system. To explore this potential, this dissertation investigates the implementation feasibility of COB transportation within the U.S. intermodal freight transportation system. Three contributions …

A Grounded Theory Study Of White Educators' Conceptualizations And Negotiations Of Culturally Centered Practices In Colleges Of Agriculture And Related Sciences, Isabel M. Whitehead-Adams

Graduate Theses and Dissertations

The racial and ethnic demographics of higher education are becoming increasingly diverse; however, faculty demographics remain predominantly White. With agriculture being one of the largest economic sectors, often with many roles to fill, recruiting and retaining racially and ethnically diverse students into agricultural and related science post-secondary programs, hereby referred to as agricultural programs, is crucial to drawing talent into the agricultural workforce. However, racial and ethnic demographics among students in post-secondary agricultural programs also remain predominantly White. Established pedagogical approaches for supporting culturally diverse have been established, such as Culturally Responsive Teaching. There is little to no research exploring …

Antiracism In Higher Education: An Integrated Psychological Perspective, Rhea E. Nesbitt

Dissertations, 2020-current

Antiracism is a complicated and important term with prominent, influential voices claiming that systemic equity and the reconfiguration of white identity are the central solutions to eradicating American racism. Within the profound sociocultural effects of the existing antiracist rhetoric, there is vagueness, conflict, and incoherence. Language serves as a fundamental aspect of human experience, facilitating individual meaning-making and comprehension. Effective communication hinges on the ability to articulate subjective experiences and justifications clearly, a challenge evident in discussions surrounding antiracism. Despite advocating for antiracism as crucial and unending work, scholars recognize significant hurdles, including unclear responsibilities for implementation and entrenched structures …