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Neural And Computational Approach To Understanding Environmental Modulation Of Behavioral Identity In Zebrafish, John W. Hageter

Graduate Theses, Dissertations, and Problem Reports (ETD)

Organisms rely on behavior for survival. Animals engage in behaviors that allow for feeding, mating, exploring and navigating their environment among others. Necessary for these behaviors to develop are the environmental factors and underlying circuitry which make behavior possible. Specifically, how the environment guides underlying neural circuitry to develop unique facets or phenotypes of a larger behavior are key to understanding why unique behaviors exist. In this thesis, I build foundational evidence for determining these mechanisms through the use of the zebrafish local search behavior. This is a behavior that zebrafish employ following the loss of environmental illumination where they …

Engaging Young Children In Art Museums Through Interactive Programming, Rebekah K. Bostic

Graduate Theses, Dissertations, and Problem Reports (ETD)

Young children can be successfully engaged in art museum programs in many ways. Rather than identifying only one pedagogical strategy that is most effective for them, it is more productive to consider how different approaches affect the experiences of children in a museum. Despite the considerable body of existing research about educational strategies for preschool children, a clear synthesis of various pedagogical methods has not been applied to art museum programming for this age group. This research project used participant observation as its methodology to conduct four case studies attempting to answer the question: “How do different museum educational strategies …

Reprogramming Of Immunometabolic Functions In Brain Microvascular Endothelial Cells During Neuroinflammation: Insights From Ischemic Stroke And Sepsis, Shokofeh Rahimpourkaldeh

Graduate Theses, Dissertations, and Problem Reports (ETD)

Ischemic stroke and sepsis-associated encephalopathy (SAE) are two distinct conditions that share common pathophysiological mechanisms affecting brain microvascular endothelial cells (BMECs). Ischemic stroke results from an acute reduction in cerebral blood flow due to thrombosis or embolism, leading to energy failure, oxidative stress, and blood-brain barrier (BBB) dysfunction. SAE, a neurological complication of sepsis, arises from systemic inflammation, endothelial activation, and BBB disruption, leading to cognitive impairment and neuroinflammation. Despite their different etiologies, both conditions involve BMEC dysfunction as a central mediator of neurovascular injury. This dissertation aims to investigate the immunometabolic dysfunction of BMECs in ischemic stroke and sepsis, …

A Post Apocalyptic Romp: Designing Costumes For Phoebe In Winter, Emmalee Berger

Graduate Theses, Dissertations, and Problem Reports (ETD)

This thesis will document the process of costume designing for Phoebe in Winter by Jen Silverman. It will examine the process from first read of the script through initial and final design meetings and to execution and production of the design. This will report on challenges specific to this production, such as nudity and violence on an academic stage, and the subsequent solutions. Phoebe in Winter was produced by West Virginia University School of Theatre and Dance in October of 2024. The design process began in February of the same year.

Understanding The Prevalence Of Γ-Hydroxybutyric Acid In Hair For Clinical And Forensic Applications, Collin J. Kustera

Graduate Theses, Dissertations, and Problem Reports (ETD)

Sexual assault affects over 400,000 Americans annually, including 13% of college students, yet only 2.5% of assailants face incarceration. This disparity stems from significant challenges in evidence collection, particularly in drug-facilitated crimes (DFCs). Victims often delay reporting due to fear, self-blame, or unawareness of the assault which is common in cases involving substances like γ-hydroxybutyric acid (GHB). Current forensic standards rely on blood and urine, which offer detection windows of just 48 and 120 hours, respectively. These short timeframes frequently fail to capture critical toxicological evidence, leaving investigators with only victim testimony, which the judicial system struggles to act upon …

Development Of Sexual Health Curriculum For West Virginia University’S Occupational Therapy Program, Abigail Jeske

Graduate Theses, Dissertations, and Problem Reports (ETD)

Sexual health is a fundamental aspect of well-being and quality of life and impacts everyone regardless of age, gender, or sexual orientation, and is relevant throughout an individual’s lifespan. Occupational therapists have a valuable role in addressing sex concepts with patients; however, a lack of education and comfort level have been identified as barriers to practitioners addressing the concepts in clinical practice. This Doctoral Capstone Experience aims to answer how the topic of sexual health can be integrated into a lifespan curriculum in formal occupational therapy education. A sexual health curriculum was created and integrated into seven different classes that …

Romantic Materialities And The Politics Of Care: Negotiating Movement In A More-Than-Human World, April J. Mcginnis

Graduate Theses, Dissertations, and Problem Reports (ETD)

This dissertation explores the ecological politics of movement in Romantic literature. It contributes to a growing body of scholarship that investigates the important role that walking played in nineteenth-century literary culture. Many authors from this period cast walking as a practice that frees up not only the body but the mind as well, as the steady rhythm of the feet lulls the walker into a state of self-absorbed introspection and heightened creativity. Because of this, walking was a celebrated part of the creative process for many writers. The freedom they celebrate is possible, however, only in spaces that are level, …

A Learning History Of Online Portfolio Development In Kinesiology: Academic Innovation From Pilot To Powerhouse, Lyndsay M. Burneisen Phd

Graduate Theses, Dissertations, and Problem Reports (ETD)

This study examines the phased development of an online kinesiology education program, tracing its evolution from inception to institutionalization through Rogers’ Diffusion of Innovations (DOI) theory. Using a learning history methodology, this research synthesizes document analysis, stakeholder interviews, and focus groups, and historical program data to explore how strategic leadership, faculty engagement, student support, and technological advancements influenced the program’s growth and sustainability. The research is structured around three critical phases: inception, maturation, and institutionalization, highlighting key adaptations in curriculum design, administrative structures, and quality assurance. Findings reveal a transition from a decentralized, experimental model to a structured, integrated online …

Adminstrative And Programmatic Supports To Enhance Veteran-Service Dog Programming, Madison Davisson

Graduate Theses, Dissertations, and Problem Reports (ETD)

Background: Service dogs have long served as a valuable resource to assist veterans in overcoming challenges such as transitioning to post-military life, re-engaging in life roles, and navigating the community. There is substantial research on the benefits and impacts that service dogs have on the lives of veterans. Because of this, the number of service dog-veteran partnerships is on the rise, and each service dog program has its individualized programmatic method to enhance its offerings. However, there is limited information on administrative and programmatic support for service dog training programs intended for veterans and staff members. The veteran perspective is …

A Comparison Of The Strategies And Methods Influencing Success Among Formally Trained Artists V. Outsider, Naive, Or Self-Taught Artists, Anna Stolina

Graduate Theses, Dissertations, and Problem Reports (ETD)

This thesis examines the contrasting strategies and methods formally trained artists, self- taught artists, naive artists, and outsider artists used to influence their success in their chosen field. The study explored each of their educational backgrounds, available resources, and unique expertises that allowed them to achieve a successful art career. Every pathway has unique advantages and challenges (Langley, 2024). Formally trained artists and exhibiting artists typically have degrees in art from a university or post-secondary art school. My research highlighted the benefits of having a formal art education, a structured learning environment, access to resources, networking opportunities, and professional mentorship. …

Comparison Of Compositional Characteristics Of Protein Extracted From Spent Hemp Seed Cakes By One-Step Defatting With Organic Solvents Followed By Isoelectric Solubilization Precipitation Using Strong And Weak Acids, Miah Nicole Gathman

Graduate Theses, Dissertations, and Problem Reports (ETD)

Hemp seed cakes (HSC) are the byproduct of pressing hemp seeds for oil and are a significant agricultural waste product, with hundreds of tons produced annually. These nutrient-rich cakes have potential for sustainable applications in animal feed and food products. However, they could offer even greater value to the food industry through further extraction of the lipids and separation of the proteins they contain. Protein is a key nutritional component and companies are seeking cost-effective, time-efficient processes to concentrate protein from underutilized resources like HSC. Hexane (HEX) is a common solvent used in one-step defatting processes but may cause issue …

Peace Education: Exploring Educational Possibilities, Erica Ferrer Ariza

Graduate Theses, Dissertations, and Problem Reports (ETD)

Peace Education is a growing field of study that includes different approaches to understanding and promoting peace, conflict resolution, and social justice. Peace education can be a strategic area for societies that have experienced various forms of direct or indirect violence as is the case of Colombia, South America, which has undergone prolonged periods of violence and internal conflict. Considering that peace is contextual and should be defined and decided on by the people involved in the target context, this work aims to illuminate how a peace education process can be experienced by teachers and students and the forms it …

Manualized Interventions For Individuals Experiencing Or At-Risk For Adverse Life Experiences, Sierra P. Mcnicol

Graduate Theses, Dissertations, and Problem Reports (ETD)

Background: Occupational practitioners have a role in providing holistic and client-centered care to promote occupational performance, engagement, and satisfaction through occupations. Many individuals are impacted in the engagement of occupations secondary to adverse life experiences that have caused mental distress, difficulties in cognitive processing, limited social support, and disruptions in social determinants that have made accessing services complicated. Purpose: Due to the increased likelihood of individuals who have experienced adverse life experiences experiencing occupational distress and performance limitations, the capstone student aimed to develop an understanding of adverse life experiences, provide insight into the needs of the population, and develop …

A Comparative Study On The Accuracy And Reliability Of Artificial Intelligent Lateral Cephalogram Tracing Programs, Stuart Mills

Graduate Theses, Dissertations, and Problem Reports (ETD)

Objective: To compare the accuracy and reliability of two commercially available Artificial Intelligent (AI) cephalogram tracing software programs to doctor traced cephalograms.

Materials and Methods: A sample of 100 pretreatment cephalograms were analyzed with AI based software programs, Angel Aligner iCeph and WebCeph, then compared to images traced by an orthodontist using Dolphin Imaging. Of the 100 samples, 50 were skeletally Class I and 50 were skeletally Class II. For each cephalogram produced, the American Board of Orthodontics 11 measurements were acquired from each of the three tracing programs. One way analysis of variance (ANOVA) followed by Tukey’s HSD test …

Quantifying Subsurface Pavement Temperatures Subject To Future Climates And Implications For Pavement Resilience, Austin Michael Jarrell

Graduate Theses, Dissertations, and Problem Reports (ETD)

Assessment of anthropogenic climate change impacts in pavement design and analysis remains limited within engineering practice, despite growing recognition of the need for climate-resilient roadway infrastructure. Research efforts have increasingly focused on integrating future climate projections into mechanistic-empirical pavement design, which represents the leading edge of the state of practice. However, a lack of consensus persists regarding the optimal methods for incorporating climate projections, particularly in the selection of downscaling techniques and global climate models (GCMs) to ensure accuracy and applicability for pavement performance analysis. This research investigates the impact of statistical downscaling methodologies and GCM selection routines on subsurface …

No Sweeter Sound: Musicking, Transformative Pedagogy, And Collaborative Social Justice In Choral Settings, Natalie N. Shaffer

Graduate Theses, Dissertations, and Problem Reports (ETD)

Choral music and its conductors occupy a tenuous space in the 21st century. In a new cultural atmosphere influenced by Gen Z’s expectation of welcomed diversity and intolerance for injustice, TikTok’s rapid global dissemination of information and entertainment, and society’s desire for authentic connection following the Covid-19 pandemic, the dichotomies present in choral music are no longer being glossed over. Difficult conversations about race, gender, ableism, social location, and other intersectional identifiers are at the forefront of academic writing and conference presentations. Evidence of transformative pedagogy and culturally responsive practices are beginning to appear in classroom syllabi and rehearsal techniques …

Xanthine Oxidoreductase, Uric Acid, And Iron Homeostasis: Discovering A Therapeutic Vulnerability In Breast Cancer, Matthew G. Chapa

Graduate Theses, Dissertations, and Problem Reports (ETD)

Breast cancer remains the most frequently diagnosed malignancy and the leading cause of cancer related deaths in women. This is primarily due to distant metastases, which highlight the necessity to understand factors that contribute to breast cancer progression. Emerging evidence associates decreased tumor xanthine oxidoreductase (XOR) expression with a poorer prognosis, increased recurrence, and a more aggressive phenotype. While healthy liver, intestines, kidneys, and breast tissue have robust XOR expression and activity, there is a tendency for this to be ablated in carcinogenesis. This dissertation investigates XOR's role in breast cancer and identifies its product, uric acid (UA), as possessing …

Comparative Analysis Of Safety, Accuracy, Comprehensiveness, And Organization In Endodontic Patient Education Material: American Association Of Endodontics (Aae) Vs. Ai-Generated Content., Fabiola M. Salgado Reyes

Graduate Theses, Dissertations, and Problem Reports (ETD)

Comparative Analysis of Safety, Accuracy, Comprehensiveness, and Organization in Endodontic Patient Education Material: American Association of Endodontics (AAE) vs. AI-Generated Content. Fabiola Salgado Reyes, DMD Introduction: With patients increasingly using AI chatbots like ChatGPT4o and Copilot, it is essential to assess whether these tools can effectively support patient understanding of complex endodontic procedures. AI-generated responses may occasionally provide inaccurate, unsafe, disorganized, or content that is not comprehended. The purpose of this study is to examine if dental experts and patients can determine if patient education materials (PEMS) were 1) generated by AI; 2) medically safe; 3) accurate; 4) can be …

Does Education On Aseptic Non-Touch Technique Affect Nurse Knowledge On Aseptic Technique When Placing Ultrasound Guided Peripheral Intravenous Lines? A Quality Improvement Initiative, Hannah G. Keyser

Graduate Theses, Dissertations, and Problem Reports (ETD)

This Doctor of Nursing Practice (DNP) project attempted to increase nurse’s knowledge of aseptic technique when placing USG-PIVs in hospitalized adult patients after educational training on the Aseptic Non-Touch Technique Clinical Practice Framework (ANTT CPF). Implementation of the ANTT CPF in the cardiovascular intensive care unit (CVICU) at Ruby Memorial Hospital, as a didactic educational session for nurses already trained to place ultrasound-guided peripheral intravenous lines, was utilized for this DNP project. Pre- and post-surveys were employed to determine changes in knowledge scores as well as feelings of comfort and knowledge related to the CPF. Results remained stable for survey …

Searching For The "Tender Places": Shoddy, Knowledge, And Material Culture In The Civil War Era, Robert William Novak

Graduate Theses, Dissertations, and Problem Reports (ETD)

In the American Civil War era, shoddy was difficult to understand. It was simultaneously seen as evidence of advancing western civilization as well as a disgusting amalgamation of refuse and disease that threatened those who touched it. Made from shredding woolen rags and tailors’ clippings, the recycled woolen fibers could by applied in myriad woolen blends that came to dominate the ready-made clothing industry that developed through the first half of the nineteenth century. Its application could soften coarse blends and refine the appearance of cheap cloth. When the American Civil War erupted in 1861, however, shoddy came to define …

Expanding The Vibrating Sharp-Edge Spray Ionization Toolkit For Oligonucleotide Characterization, Sultan Mahmud

Graduate Theses, Dissertations, and Problem Reports (ETD)

A gentle or soft Ionization process is a critical component in native mass spectrometry (MS) to lift the biomolecules from the bulk solution into the gas phase environment of the mass spectrometer preserving their shape, topology and non-covalent interactions. Electrospray ionization (ESI) facilitates structural preservation of proteins, nucleic acids and their complexes and as a result it has been extensively used in native MS. However, in negative ion mode, ESI is somewhat limited in sensitivity due to corona discharge effect resulting from the requirement of a high amount of voltage. This often disrupts the ESI process and may not preserve …

Co-Transmission Is Co-Opted For The Coordination Of Auditory Sensory Integration, Marryn M. Bennett

Graduate Theses, Dissertations, and Problem Reports (ETD)

Corollary discharge circuits (CDCs) are conserved mechanisms for sensory modulation during species specific behavioral programs. Many behaviors require multiplexed differential sensory modulation for proper execution. Co-transmission of fast and slow neurotransmitters allows for distinct modulation of different downstream targets within one sensorimotor circuit. However, studying co-transmission within the context of modulatory circuits like CDCs requires complete knowledge of behavioral circuits and precise tools for individually determining the implications of each transmitter on its targets. In this dissertation, I used a conserved set of corollary discharge interneurons in Drosophila melanogaster as a model to study the intricacies of sensory modulation through …

An Evidence-Based Practice Initiative To Improve Blood Pressure Control, Teresa D. Hines

Graduate Theses, Dissertations, and Problem Reports (ETD)

Introduction/Background: High blood pressure (HBP) is a significant public health concern. It affects millions of adults in the United States (US) and half of the adult population in the Appalachia regions. High blood pressure is a known risk factor for cardiovascular disease (CVD), which is the leading cause of morbidity and mortality Appalachia. Complications associated with high blood pressure result in substantial economic burdens for patients and their families, healthcare systems, and national economies. High blood pressure is a significant health issue in West Virginia (WV), with an estimated 602,000 people suffering from HBP.

Purpose: The purpose of this …

Introduction Of Polar Functional Groups To Pyridinedipyrrolide Zirconium Photosensitizers Through Post-Synthetic Modifications, Marisa Nicole Tordella

Graduate Theses, Dissertations, and Problem Reports (ETD)

Photosensitizers are vital components to applications such as photoredox catalysis, photodynamic therapy, and various avenues of solar energy production. The abilities of transition metal photosensitizers containing precious metals have been well studied over the years. However, because of the growing scarcity of these metals, there has been a shift in finding more earth abundant alternatives. To help fill this gap in the literature, the Milsmann group has developed and studied the photophysical properties of Zr(IV) complexes with various ligand systems. Due to the electron deficient metal center, these complexes exhibit ligand-to-metal charge transfer (LMCT), unlike the metal-to-ligand charge transfer commonly …

Three Essays On Environmental Regulation And Economic Revitalization In Energy Rich Regions, Young Gwan Lee

Graduate Theses, Dissertations, and Problem Reports (ETD)

This dissertation consists of three essays that investigate the environmental and regulatory dimensions of unconventional oil and gas (UOG) development and place-based policy in the United States. The research examines how energy production activities interact with water resource management, regulatory compliance behavior, and regional economic resilience. By integrating empirical modeling, quasi-experimental methods, and policy evaluation, the dissertation provides new insights into how environmental risks and economic tradeoffs are shaped by regulatory constraints, strategic behavior of regulated entities, and government interventions in extractive regions. Managing produced water from unconventional oil and gas (UOG) production is a growing environmental challenge, particularly as …

Monitoring West Virginia Wastewater For Norovirus: A Test Case For Tracking Foodborne Illness Outbreaks In Rural Communities Using Wastewater, Haley M. Phillippi

Graduate Theses, Dissertations, and Problem Reports (ETD)

Foodborne illnesses are a global public health challenge. Foodborne illnesses, like norovirus, often have a large disease and societal burden. Norovirus is a single-stranded, positive-sense RNA virus, with 10 identified genogroups (GI-GX). Genogroups GI and GII are responsible for nearly all human cases of acute gastroenteritis worldwide. In the United States, HuNoV is not notifiable case-by-case. Instead, outbreaks are reported to national surveillance systems when two or more cases are epidemiologically linked. Most norovirus outbreaks occur during Winter to early Spring, however, certain individuals may still shed and transmit the virus without being detected or reported. Wastewater monitoring is a …

Evaluation And Identification Of Novel Viruses In Wild Fishes Using Varied Molecular Biology Techniques, Clayton Douglas Raines

Graduate Theses, Dissertations, and Problem Reports (ETD)

Viruses are among the most abundant biological entities on Earth, yet their diversity and ecological roles in aquatic vertebrates, particularly fishes, remain poorly characterized. This dissertation advances fish virology through integrative approaches that combine historical context, molecular diagnostics, and genomic discovery. A novel hepadnavirus (ApHBV) was identified in asymptomatic Alosa pseudoharengus, emphasizing the prevalence of subclinical infections in migratory forage species. In Catostomus commersonii, transcriptomic profiling revealed sexually dimorphic immune responses to hepatitis B and C-like viruses, highlighting complex host-pathogen dynamics. In Micropterus nigricans, next-generation sequencing uncovered a novel nackednavirus and multiple adomaviruses within hyperpigmented lesions, expanding …

The Dna Methylation Capacity Of The Tsetse Fly: Glossina Morsitans, And Its Integration With Symbiont Nutrient Provisioning, Dylan Forrest Richmond

Graduate Theses, Dissertations, and Problem Reports (ETD)

Tsetse flies (Diptera: Glossinidae) are the vector of trypanosomes (Trypanosoma spp.), which are responsible for African trypanosomiasis, also known as sleeping sickness. African trypanosomiasis is a significant health and economic burden yet remains a neglected tropical disease. As an obligate blood feeder, tsetse flies are reliant on B vitamin supplementation from intracellular Gammaproteobacteria, Wigglesworthia glossinidia. Folate (B9) is essential for tsetse development and reproduction, as well as DNA methylation in animals. As a reversible modification, DNA methylation typically occurs in a CpG dinucleotide context and can alter gene expression via the restriction of transcription. Across insects, the …

Deep Target Recognition: Semi-Supervised Annotation, Sensor Fusion And Super-Resolution, Shoaib Meraj Sami

Graduate Theses, Dissertations, and Problem Reports (ETD)

Despite the recent expansion of machine learning algorithms to cover a wide range of disciplines, several areas of automatic target recognition (ATR) remain underexplored. This dissertation presents tools developed to improve performance in three significant aspects of ATR: semi-supervised annotation, sensor fusion, and image super-resolution. The aim of the semi-supervised methods is to automatically annotate targets in scenarios where labeled data are scarce in the target domain but available in the source domain. Secondly, to address the limitations of individual image sensors and enhance robustness under different environmental conditions and man-made constraints, a sensor fusion algorithm was developed to improve …

Ciliary Arl14b In Vision, Alexis Breann Crockett

Graduate Theses, Dissertations, and Problem Reports (ETD)

Primary cilia are highly specialized organelles that regulate signaling, trafficking, and sensory perception across multiple tissues, including the retina. The small GTPase ADP Ribosylation Factor-Like Protein 13B (ARL13B) is an atypical ARF-family protein enriched in cilia. ARL13B acts as a guanine exchange factor (GEF) for another small GTPase ARF-like Protein 3 (ARL3). Mutations in ARL13B cause Joubert syndrome, a ciliopathy characterized by brain malformation, developmental delays, and retinal degeneration. Despite ARL13B’s known ciliary localization and clinical relevance, ARL13B’s precise functions in cilia remain poorly defined. This dissertation investigates the cilia-specific roles and GEF-specific roles of ARL13B in photoreceptors using two …