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Articles 39031 - 39060 of 1103433
Full-Text Articles in Entire DC Network
Hourly Simulated Power Production Data With No Snow Loss Model At Queued Utility-Scale Pv Sites Simulated As Single-Axis Tracking Systems In The U.S. Eastern Interconnection For Weather Year 2019, Shelbie Wickett, Ana Dyreson
Hourly Simulated Power Production Data With No Snow Loss Model At Queued Utility-Scale Pv Sites Simulated As Single-Axis Tracking Systems In The U.S. Eastern Interconnection For Weather Year 2019, Shelbie Wickett, Ana Dyreson
Simulated Solar Capacity Including Snow Cover in the Eastern U.S.
Using 2019 weather data, we ran PySAM power production simulations for utility-scale PV sites in the U.S. Eastern Interconnection queue. Site IDs, capacities, and locations (counties) were extracted from Lawrence Berkeley National Laboratory's Queued Up: 2024 Edition dataset. No panel mount information was provided, so all sites were assumed to be the centers of the installation counties. See queued_site_metadata.csv file for individual site metadata
Anionic Azo Dyes: Wastewater Pollutants As Functionalizing Agents For Porous Polycarbonate Membranes Aiding In Water Decolorization, Alan Jarrett Messinger, Isabella S. Mays, Brennon Craigo, Jeffrey Joering, Sean P. Mcbride
Anionic Azo Dyes: Wastewater Pollutants As Functionalizing Agents For Porous Polycarbonate Membranes Aiding In Water Decolorization, Alan Jarrett Messinger, Isabella S. Mays, Brennon Craigo, Jeffrey Joering, Sean P. Mcbride
Physics Faculty Research
Efficient water decolorization techniques are vital for ensuring fresh water for future generations. Azo dyes are used heavily in the textile industry and are a challenge to remove from industrial wastewater. This research expands on recent innovative work where anionic azo dyes themselves were used to functionalize track-etched porous polycarbonate filtration membranes with decolorized water obtained as a byproduct. The objective of this research is to determine whether the observed dye rejection is dependent on the magnitude of the intrinsic charge of the dye molecule or on its structure, using two selectively chosen anionic azo dye series during functionalization. The …
Projections Of Lightning‐Ignited Wildfire Risk In The Western United States, Dmitri A. Kalashnikov, John T. Abatzoglou, Frances V. Davenport, Zachary M. Labe, Paul C. Loikith, Danielle Touma, Deepti Singh
Projections Of Lightning‐Ignited Wildfire Risk In The Western United States, Dmitri A. Kalashnikov, John T. Abatzoglou, Frances V. Davenport, Zachary M. Labe, Paul C. Loikith, Danielle Touma, Deepti Singh
Geography Faculty Publications and Presentations
Cloud‐to‐ground (CG) lightning is a major source of summer wildfire ignition in the western United States (WUS). However, future projections of lightning are uncertain since lightning is not directly simulated by most global climate models. To address this issue, we use convolutional neural network (CNN)‐based parameterizations of daily June‐September CG lightning. CNN parameterizations of daily CG lightning occurrence at each grid cell use fields of three thermodynamic variables—ratio of surface Moist Static Energy (MSE) to 500 hPa saturation MSE, 700–500 hPa lapse rate, and 500 hPa relative humidity. Applying these parameterizations to the Community Earth System Model version 2 Large …
Exploring The Moderating Effect Of Boundary Management Strategy In A Remote Work Environment, Brandon M. Vasquez
Exploring The Moderating Effect Of Boundary Management Strategy In A Remote Work Environment, Brandon M. Vasquez
Master's Theses
Remote work has evolved significantly, especially during the COVID-19 pandemic, and continues to influence employee experiences in both positive and negative ways. Managing the separation between work and personal life is of importance. More specifically, using a boundary management strategy of segmentation may be more effective than an integration strategy because those who separate the two domains of work and family/personal life may be less prone to conflict in their lives. The present study aimed to examine the moderating effect of boundary management strategy on the relationship between remote work and job satisfaction, work-life balance, and well-being. Specifically, it was …
Artificial Intelligence In Ocular Transcriptomics: Applications Of Unsupervised And Supervised Learning, Catherine Lalman, Yimin Yang, Janice L. Walker
Artificial Intelligence In Ocular Transcriptomics: Applications Of Unsupervised And Supervised Learning, Catherine Lalman, Yimin Yang, Janice L. Walker
Department of Pathology, Anatomy, and Cell Biology Faculty Papers
Transcriptomic profiling is a powerful tool for dissecting the cellular and molecular complexity of ocular tissues, providing insights into retinal development, corneal disease, macular degeneration, and glaucoma. With the expansion of microarray, bulk RNA sequencing (RNA-seq), and single-cell RNA-seq technologies, artificial intelligence (AI) has emerged as a key strategy for analyzing high-dimensional gene expression data. This review synthesizes AI-enabled transcriptomic studies in ophthalmology from 2019 to 2025, highlighting how supervised and unsupervised machine learning (ML) methods have advanced biomarker discovery, cell type classification, and eye development and ocular disease modeling. Here, we discuss unsupervised techniques, such as principal component analysis …
Effects Of Cryoprotective And Mitochondrial Stabilizing Agents On Vitrified Bovine Oocytes, Allison Hoang
Effects Of Cryoprotective And Mitochondrial Stabilizing Agents On Vitrified Bovine Oocytes, Allison Hoang
LSU Master's Theses
Oocyte vitrification success is highly dependent on cryoprotectant agent (CPA) choice as well as possible additives that could help preserve mitochondrial membrane potential (mMP), reactive oxygen species (ROS) production, and calcium homeostasis within the cell. It is a delicate balance between levels needed for proper oocyte function and activation and levels needed to induce apoptotic pathways. The results of oocyte cryopreservation determine the eventual oocyte viability and subsequent fertilization and embryo development.
In this study potential permeating cryoprotectant combinations were explored such as ethylene glycol (EG) and propylene glycol (PG) or EG in combination with dimethyl sulfoxide (DMSO). Also, the …
Investigation Of Co-Current And Counter-Current Multiphase Flow In Upstream Drilling And Production Applications, Hazem Fleifel
Investigation Of Co-Current And Counter-Current Multiphase Flow In Upstream Drilling And Production Applications, Hazem Fleifel
LSU Doctoral Dissertations
In the domain of upstream Petroleum Engineering, the dynamics of co-current and counter-current multiphase flows present huge challenges, significantly impacting the efficiency of associated processes. This study investigates two specific applications concerning these flow behaviors, involving both laboratory experiments and transient computer simulations. This work investigates two distinct well control and production enhancement processes, involving multiphase flow dynamics: the bullheading kill procedure and liquid-assisted gas lift (LAGL).
The first topic (Topic 1) explores the bullheading process, a type of well control methods, by challenging the traditional understanding that gas removal efficiency increases monotonically with injection pump flow rate. Through 1D …
Navigating The Challenges Of Global & National Uncertainty, The Global & National Security Policy Institute
Navigating The Challenges Of Global & National Uncertainty, The Global & National Security Policy Institute
LAII Events
Tuesday, August 26, 2025 | 08:00 am - 05:00 pm
Student Union Building Ballroom C
About:
The Global & National Security Policy Institute will gather the New Mexico security community for a symposium featuring practitioner and academic perspectives on the challenges of intelligence analysis, grand strategy, extended deterrence in Taiwan, the nuclear age, technology and advancement, and global cooperation for enhancing non-traditional security as well as poster presentations by GNSPI's Junior Research Fellows.
Notes:
This event is free and open to the public.
The Impact Of Interactive Classroom Interior Design On Student’S Perceived Well-Being: A Case Study Of Egyptian Language Schools In Cairo., Dalia Moati, Tasneem Gamal, Rodina Mostafa, Yara Farrag
The Impact Of Interactive Classroom Interior Design On Student’S Perceived Well-Being: A Case Study Of Egyptian Language Schools In Cairo., Dalia Moati, Tasneem Gamal, Rodina Mostafa, Yara Farrag
Architectural Engineering
The impact of classroom interior design on students’ perceived well- being, encompassing cognitive, physical, and social aspects, is substantial. While there is increasing evidence supporting the importance of individual interior elements such as lighting, colour, and furniture, most research examines these factors separately, neglecting their combined effects. This research seeks to examine how an integrated approach to interactive classroom interior design through flexible seating areas, ergonomic furniture, natural lighting, colours psychology, acoustics, and thermal comfort influences student well- being (physical, social, and cognitive). The research explores the most effective combinations of design components in fostering a positive and supportive learning …
Utilization Of Colors, Lighting, And Materials As A Narrative Ai-Tool To Convey Human Emotions In Museums’ Interior Spaces. A Case Study Of The Museum Of Islamic Art Cairo, Egypt., Dalia Moati, Rawan Medhat, Fagr Hesham, Nada Khaled, Tamer
Utilization Of Colors, Lighting, And Materials As A Narrative Ai-Tool To Convey Human Emotions In Museums’ Interior Spaces. A Case Study Of The Museum Of Islamic Art Cairo, Egypt., Dalia Moati, Rawan Medhat, Fagr Hesham, Nada Khaled, Tamer
Architectural Engineering
This study explores the relationship between museum interior design and human emotional responses, focusing on how key design elements—color, lighting, and materials—can be optimized to enhance visitor engagement and emotional well- being. Conducted at the Museum of Islamic Art in Cairo, Egypt, the research examines two critical spaces along the visitor journey: the Medicine Hall, introducing the experience, and the Writings Hall, situated toward the end of the route, where fatigue and emotional disengagement often occur. Over a nine-week period, a mixed-methods approach was employed, integrating structured observation, behavioral mapping, and AI-assisted image modifications to generate controlled design variations, alongside …
Hourly Simulated Power Production Data With No Snow Loss Model At Queued Utility-Scale Pv Sites Simulated As Single-Axis Tracking Systems In The U.S. Eastern Interconnection For Weather Year 2016, Shelbie Wickett, Ana Dyreson
Hourly Simulated Power Production Data With No Snow Loss Model At Queued Utility-Scale Pv Sites Simulated As Single-Axis Tracking Systems In The U.S. Eastern Interconnection For Weather Year 2016, Shelbie Wickett, Ana Dyreson
Simulated Solar Capacity Including Snow Cover in the Eastern U.S.
Using 2016 weather data, we ran PySAM power production simulations for utility-scale PV sites in the U.S. Eastern Interconnection queue. Site IDs, capacities, and locations (counties) were extracted from Lawrence Berkeley National Laboratory's Queued Up: 2024 Edition dataset. No panel mount information was provided, so all sites were assumed to be the centers of the installation counties. See queued_site_metadata.csv file for individual site metadata
Hourly Simulated Power Production Data With No Snow Loss Model At Queued Utility-Scale Pv Sites Simulated As Single-Axis Tracking Systems In The U.S. Eastern Interconnection For Weather Year 2018, Shelbie Wickett, Ana Dyreson
Hourly Simulated Power Production Data With No Snow Loss Model At Queued Utility-Scale Pv Sites Simulated As Single-Axis Tracking Systems In The U.S. Eastern Interconnection For Weather Year 2018, Shelbie Wickett, Ana Dyreson
Simulated Solar Capacity Including Snow Cover in the Eastern U.S.
Using 2018 weather data, we ran PySAM power production simulations for utility-scale PV sites in the U.S. Eastern Interconnection queue. Site IDs, capacities, and locations (counties) were extracted from Lawrence Berkeley National Laboratory's Queued Up: 2024 Edition dataset. No panel mount information was provided, so all sites were assumed to be the centers of the installation counties. See queued_site_metadata.csv file for individual site metadata
Hourly Simulated Power Production Data With Snow Loss Model At Existing Utility-Scale Pv Sites (>5 Mw) In The U.S. Eastern Interconnection In 2013, Shelbie Wickett, Ana Dyreson
Hourly Simulated Power Production Data With Snow Loss Model At Existing Utility-Scale Pv Sites (>5 Mw) In The U.S. Eastern Interconnection In 2013, Shelbie Wickett, Ana Dyreson
Simulated Solar Capacity Including Snow Cover in the Eastern U.S.
Project Summary: We ran PySAM power production simulations for utility-scale (>5 MW) PV sites located in the U.S. Eastern Interconnection in the year 2013. Site panel mounts (fixed-tilt or single-axis tracking), capacities, and locations (latitudes and longitudes) were extracted from Lawrence Berkeley National Laboratory's Utility-Scale Solar 2024 Edition dataset. See 2013_PV_existing_site_metadata.csv file for individual site metadata.
Hourly Simulated Power Production Data With Snow Loss Model At Existing Utility-Scale Pv Sites (>5 Mw) In The U.S. Eastern Interconnection In 2014, Shelbie Wickett, Ana Dyreson
Hourly Simulated Power Production Data With Snow Loss Model At Existing Utility-Scale Pv Sites (>5 Mw) In The U.S. Eastern Interconnection In 2014, Shelbie Wickett, Ana Dyreson
Simulated Solar Capacity Including Snow Cover in the Eastern U.S.
Project Summary: We ran PySAM power production simulations for utility-scale (>5 MW) PV sites located in the U.S. Eastern Interconnection in the year 2014. Site panel mounts (fixed-tilt or single-axis tracking), capacities, and locations (latitudes and longitudes) were extracted from Lawrence Berkeley National Laboratory's Utility-Scale Solar 2024 Edition dataset. See 2014_PV_existing_site_metadata.csv file for individual site metadata.
Hourly Simulated Power Production Data With No Snow Loss Model At Existing Utility-Scale Pv Sites (>5 Mw) In The U.S. Eastern Interconnection In 2015, Shelbie Wickett, Ana Dyreson
Hourly Simulated Power Production Data With No Snow Loss Model At Existing Utility-Scale Pv Sites (>5 Mw) In The U.S. Eastern Interconnection In 2015, Shelbie Wickett, Ana Dyreson
Simulated Solar Capacity Including Snow Cover in the Eastern U.S.
Project Summary: We ran PySAM power production simulations for utility-scale (>5 MW) PV sites located in the U.S. Eastern Interconnection in the year 2015. Site panel mounts (fixed-tilt or single-axis tracking), capacities, and locations (latitudes and longitudes) were extracted from Lawrence Berkeley National Laboratory's Utility-Scale Solar 2024 Edition dataset. See 2015_PV_existing_site_metadata.csv file for individual site metadata.
Hourly Simulated Power Production Data With No Snow Loss Model At Existing Utility-Scale Pv Sites (>5 Mw) In The U.S. Eastern Interconnection In 2016, Shelbie Wickett, Ana Dyreson
Hourly Simulated Power Production Data With No Snow Loss Model At Existing Utility-Scale Pv Sites (>5 Mw) In The U.S. Eastern Interconnection In 2016, Shelbie Wickett, Ana Dyreson
Simulated Solar Capacity Including Snow Cover in the Eastern U.S.
Project Summary: We ran PySAM power production simulations for utility-scale (>5 MW) PV sites located in the U.S. Eastern Interconnection in the year 2016. Site panel mounts (fixed-tilt or single-axis tracking), capacities, and locations (latitudes and longitudes) were extracted from Lawrence Berkeley National Laboratory's Utility-Scale Solar 2024 Edition dataset. See 2016_PV_existing_site_metadata.csv file for individual site metadata.
Hourly Simulated Power Production Data With No Snow Loss Model At Existing Utility-Scale Pv Sites (>5 Mw) In The U.S. Eastern Interconnection In 2019, Shelbie Wickett, Ana Dyreson
Hourly Simulated Power Production Data With No Snow Loss Model At Existing Utility-Scale Pv Sites (>5 Mw) In The U.S. Eastern Interconnection In 2019, Shelbie Wickett, Ana Dyreson
Simulated Solar Capacity Including Snow Cover in the Eastern U.S.
Project Summary: We ran PySAM power production simulations for utility-scale (>5 MW) PV sites located in the U.S. Eastern Interconnection in the year 2019. Site panel mounts (fixed-tilt or single-axis tracking), capacities, and locations (latitudes and longitudes) were extracted from Lawrence Berkeley National Laboratory's Utility-Scale Solar 2024 Edition dataset. See 2019_PV_existing_site_metadata.csv file for individual site metadata.
Strategies To Implement Employee Cybersecurity Awareness Programs, Felix Ngwese
Strategies To Implement Employee Cybersecurity Awareness Programs, Felix Ngwese
Walden Dissertations and Doctoral Studies
Lack of cybersecurity awareness among employees and end-users poses significant risks to organizations. This issue is critical because inadequate cybersecurity awareness increases organizational vulnerabilities, leading to financial losses and compromised data security. Grounded in the routine activity theory, the purpose of this qualitative multiple case study was to explore strategies that IT security managers had implemented to enhance employee cybersecurity awareness. The participants were six IT security managers in Cameroon, West Africa, with strategies to implement employee cybersecurity awareness. Data were collected using semistructure interviews and a review of documents. Through thematic analysis, three themes were identified: (a) cybersecurity awareness …
Exploring Cybersecurity Strategies Used To Protect Personal Health Information From Cyberattacks When Using Telemedicine, Samara Torres
Exploring Cybersecurity Strategies Used To Protect Personal Health Information From Cyberattacks When Using Telemedicine, Samara Torres
Walden Dissertations and Doctoral Studies
The healthcare industry has been impacted by an increase in cybersecurity breaches because of cyberattacks when using telemedicine. It is crucial that information technology (IT) managers in the private healthcare industry protect personal health information (PHI) to uphold patients’ right to privacy, maintain their trust, prevent legal ramifications, and to comply with government regulations. This qualitative pragmatic research used the integrated system theory (IST) as a road map to explore effective cybersecurity strategies used to protect PHI in telemedicine. The participants were seven IT managers in the private healthcare industry within the United States who had experience with cybersecurity strategies …
High-Speed Voltage Imaging Of Action Potentials In Molecular Layer Interneurons Reveals Sensory-Driven Synchrony That Augments Movement, Spencer T Brown, Meghana R Holla, Michelle A Land, Shuyuan Yang, Alex J Mcdonald, François St-Pierre, Indira M Raman
High-Speed Voltage Imaging Of Action Potentials In Molecular Layer Interneurons Reveals Sensory-Driven Synchrony That Augments Movement, Spencer T Brown, Meghana R Holla, Michelle A Land, Shuyuan Yang, Alex J Mcdonald, François St-Pierre, Indira M Raman
Faculty, Staff and Students Publications
Testing whether the synchrony of action potential firing is a cerebellar coding mechanism requires simultaneous recording, with high temporal fidelity, from populations of identified neurons. Here, we used targeted one-photon voltage imaging at 2–4 kHz to record action potentials from groups of ~10–300 molecular layer interneurons (MLIs) expressing a positively tuned, genetically encoded voltage indicator, FORCE1f or pAce. In awake resting mice, crus I MLIs fired brief (~1-ms) spikes at 20–60 spikes/s. Sensory stimuli of air puffs to the whiskers evoked short-latency (< 10 ms) increases in spiking probability. In most trials, >50% of MLIs fired synchronously with 4-ms temporal precision. The magnitude of puff-evoked whisks correlated tightly with …
Mrna Vaccines Encoding Variant Forms Of Sm-Tsp-2 Confer Protective Immunity Against Schistosoma Mansoni, Athos Silva Oliveira, Sevan Alwan, Philip T Loverde, Ramiz Shaheed, Pranav Mandyam, Rakesh Adhikari, Peter Hotez, Maria Elena Bottazzi, Jeroen Pollet
Mrna Vaccines Encoding Variant Forms Of Sm-Tsp-2 Confer Protective Immunity Against Schistosoma Mansoni, Athos Silva Oliveira, Sevan Alwan, Philip T Loverde, Ramiz Shaheed, Pranav Mandyam, Rakesh Adhikari, Peter Hotez, Maria Elena Bottazzi, Jeroen Pollet
Faculty, Staff and Students Publications
Despite the global burden of helminth infections, no human vaccines have yet been licensed against these parasites. This study explored the development and evaluation of mRNA vaccine candidates targeting Schistosoma mansoni tetraspanin-2 (Sm-TSP-2), an antigen currently under evaluation as a protein vaccine. We designed constructs encoding either full-length Sm-TSP-2, or its large extracellular loop (EC2) domain in secretory, membrane-anchored, or cytosolic forms. In a murine challenge model, the secreted and membrane-anchored versions of Sm-TSP-2-EC2 induced the highest of antigen-specific antibody titers. These two construct designs, along with full-length Sm-TSP-2 mRNA, also significantly reduced adult worm …
Effects Of Ambient Temperature On Nox Emissions From Heavy-Duty Diesel Vehicles Measured In Utah, Amber L. Gurecki Allen, Darrell B. Sonntag, Gary A. Bishop
Effects Of Ambient Temperature On Nox Emissions From Heavy-Duty Diesel Vehicles Measured In Utah, Amber L. Gurecki Allen, Darrell B. Sonntag, Gary A. Bishop
Fuel Efficiency Automobile Test Publications
This study investigates the effects of ambient temperature on NOx (NO + NO2) emissions from model year 2011 and later heavy-duty (HD) diesel vehicles. Emission measurements were collected in Perry, Utah, using the Fuel Efficiency Automobile Test (FEAT) remote sensing device. Data were limited to model year 2011 and later to focus on vehicles likely equipped with selective catalytic reduction (SCR) systems, which control tailpipe NOx emissions and are shown to be temperature sensitive. HD diesel vehicles measured in the winter of 2020 had consistently higher NOx emissions than those measured in the summer of 2023, most significantly for vehicles …
The Role Of Recruited Adipose Stromal Cells And Their Fibroblastic Differentiation In Cancer, Lingyi Cai, Mikhail G Kolonin, Dimitris Anastassiou
The Role Of Recruited Adipose Stromal Cells And Their Fibroblastic Differentiation In Cancer, Lingyi Cai, Mikhail G Kolonin, Dimitris Anastassiou
Faculty, Staff and Student Publications
Adipose stromal cells (ASCs) are perivascular mesenchymal progenitors of adipose tissue. In cancer patients, ASCs can mobilize and migrate to the tumor, where they subsequently play an important role in cancer progression. This biological process involves the conversion of recruited ASCs into cancer-associated fibroblasts (CAFs). ASC-derived CAFs influence the tumor microenvironment through extracellular matrix remodeling, vascularization, and immunomodulation. These and other processes mediated by secreted paracrine factors also affect gene expression in carcinoma cells to promote the epithelial-mesenchymal transition (EMT), metabolic adaptation, survival, and invasiveness of cancer cells. ASC-derived CAFs can enhance tumor aggressiveness, accounting in part for the link …
Impact Of Mining Activities On Land Use Land Cover (Lulc) Using Geospatial Techniques In The Obuasi Municipality, Jonathan Arthur Quaye-Ballard, Samira Naa Yemoley Nsiah, Jephthah Marfo Nimoh, Titus Tienaah, Tina Dzigbordi Wemegah, Precious Adwoa Okyere
Impact Of Mining Activities On Land Use Land Cover (Lulc) Using Geospatial Techniques In The Obuasi Municipality, Jonathan Arthur Quaye-Ballard, Samira Naa Yemoley Nsiah, Jephthah Marfo Nimoh, Titus Tienaah, Tina Dzigbordi Wemegah, Precious Adwoa Okyere
Publications
Introduction: Sustainable development, which balances economic, social, and environmental factors to meet the needs of the present and future generations, is a collective responsibility. Rapid population growth and intensification of socio-economic activities are causing significant changes in Land Use Land Cover (LULC), directly impacting ecosystem services, biodiversity, and climate. The Obuasi community is already facing environmental degradation and health problems due to gold mining.
Methodology: The study assessed the spatial extent and intensity of mining activities in the Obuasi Municipality on the LULC and predicted future LULC changes due to mining activities using geospatial techniques. The LULC changes were analysed …
Peeking Inside The Black Box: Demystifying Artificial Intelligence, David J. Mulder
Peeking Inside The Black Box: Demystifying Artificial Intelligence, David J. Mulder
Faculty Work Comprehensive List
No abstract provided.
August 26, 2025 Nagpra And Tribal Relations Committee Meeting Minutes, Bryan Morey
August 26, 2025 Nagpra And Tribal Relations Committee Meeting Minutes, Bryan Morey
University NAGPRA and Tribal Relations Committee
August 26, 2025 minutes for the WKU NAGPRA and Tribal Relations Committee meeting. Meeting conducted via Zoom.
Danicamtiv Reduces Myosin's Working Stroke But Activates The Thin Filament By Accelerating Actomyosin Attachment, Brent Scott, Lina Greenberg, Caterina Squarci, Kenneth S Campbell, Michael J Greenberg
Danicamtiv Reduces Myosin's Working Stroke But Activates The Thin Filament By Accelerating Actomyosin Attachment, Brent Scott, Lina Greenberg, Caterina Squarci, Kenneth S Campbell, Michael J Greenberg
2020-Current year OA Pubs
Heart failure is a leading cause of death worldwide, and even with current treatments, the 5-y transplant-free survival rate is only ~50 to 70%. As such, there is a need to develop new treatments for patients that improve survival and quality of life. Recently, there have been efforts to develop small molecules for heart failure that directly target components of the sarcomere, including cardiac myosin. Danicamtiv is one of these molecules; however, its direct effects on myosin's single molecule mechanics and kinetics are not well understood. Using optical trapping techniques, stopped flow transient kinetics, and in vitro reconstitution assays, we …
Cathartocytosis: Jettisoning Of Cellular Material During Reprogramming Of Differentiated Cells, Jeffrey W Brown, Xiaobo Lin, Gabriel Anthony Nicolazzi, Xuemei Liu, Thanh Nguyen, Megan D Radyk, Joseph Burclaff, Jason C Mills
Cathartocytosis: Jettisoning Of Cellular Material During Reprogramming Of Differentiated Cells, Jeffrey W Brown, Xiaobo Lin, Gabriel Anthony Nicolazzi, Xuemei Liu, Thanh Nguyen, Megan D Radyk, Joseph Burclaff, Jason C Mills
Faculty, Staff and Students Publications
Injury causes differentiated cells to undergo massive reprogramming to become proliferative and repair tissue via paligenosis. Gastric chief cells use paligenosis to reprogram into progenitor-like spasmolytic-polypeptide-expressing metaplasia (SPEM) cells. Stage 1 of paligenosis is the downscaling of mature cell architecture via a process involving lysosomes. Here, we notice that sulfated glycoproteins are not only digested during paligenosis but also excreted into the gland. Various genetic and pharmacological approaches show that endoplasmic reticulum membranes and secretory granule cargo are also excreted and that the process proceeds in parallel with but is mechanistically independent of autophagy. Three-dimensional light and electron microscopy demonstrated …
Defrnd: Detergent-Free Reconstitution Into Native Nanodiscs With Designer Membrane Scaffold Peptides, Qian Ren, Vinay Idikuda, Baron Chanda, Et Al.
Defrnd: Detergent-Free Reconstitution Into Native Nanodiscs With Designer Membrane Scaffold Peptides, Qian Ren, Vinay Idikuda, Baron Chanda, Et Al.
2020-Current year OA Pubs
Membrane scaffold protein-based nanodiscs have facilitated unprecedented structural and biophysical analysis of membrane proteins in a near-native lipid environment. However, successful reconstitution of membrane proteins in nanodiscs requires prior solubilization and purification in detergents, which may impact their physiological structure and function. Furthermore, the detergent-mediated reconstitution of nanodiscs is unlikely to recapitulate the precise composition or asymmetry of native membranes. To circumvent this fundamental limitation of traditional nanodisc technology, we herein describe the development of membrane-solubilizing peptides to directly extract membrane proteins from native cell membranes into nanoscale discoids. By systematically protein engineering and screening, we create a class of …
Pigments, Bridge Of Disciplines, Candace Gwyther
Pigments, Bridge Of Disciplines, Candace Gwyther
Imaginings: creative practice and inquiry
What follows is an excerpt from Pigments: The Bridge of Disciplines, a practice-based Masters thesis exploring the intersection of oil painting and ceramics through shared material processes. The literature review grounds the research in theories of Meshwork, Play, Bricolage, and Path making, framing an interdisciplinary approach that challenges traditional boundaries and fosters new modes of creative expression.