Digital Commons Network^™

Preparation Of Zein-Based Nanoparticles : Nanoprecipitation Versus Microfluidic-Assisted Manufacture, Effects Of Pegylation On Nanoparticle Characteristics And Cellular Uptake By Melanoma Cells, Jitkasem Meewan, Sukrut Somani, Jamal Almowalad, Partha Laskar, Margaret Mullin, Graeme Mackenzie, Swapnil Khadke, Yvonne Perrie, Christine Dufès

School of Medicine Publications

Background: The manufacture of nanoparticles using manual methods is hampered by its challenging scaleup and poor reproducibility. To overcome this issue, the production of zein nanoparticles entrapping a lipophilic drug model, coumarin-6, by using a microfluidic system was assessed in this study. The influence of PEG density and chain length on zein nanoparticle characteristics, as well as their uptake efficacy in melanoma cancer cells, was also evaluated.

Methods: Zein nanoparticles were prepared by both manual and microfluidic approaches to allow comparison between the two processes. PEGylated zein nanoparticles with various PEG densities and chain lengths were produced by …

Microbial Stimulation Of Oxytocin Release From The Intestinal Epithelium Via Secretin Signaling, Heather A Danhof, Jihwan Lee, Aanchal Thapa, Robert A Britton, Sara C Di Rienzi

Faculty, Staff and Students Publications

Intestinal microbes impact the health of the intestine and organs distal to the gut. Limosilactobacillus reuteri is a human intestinal microbe that promotes normal gut transit, the anti-inflammatory immune system, wound healing, normal social behavior in mice, and prevents bone reabsorption. Oxytocin impacts these functions and oxytocin signaling is required for L. reuteri-mediated wound healing and social behavior; however, the events in the gut leading to oxytocin stimulation and beneficial effects are unknown. Here we report evolutionarily conserved oxytocin production in the intestinal epithelium through analysis of single-cell RNA-Seq datasets and imaging of human and mouse intestinal tissues. Moreover, …

Private Continuous Survival Analysis With Distributed Multi-Site Data, Luca Bonomi, Marilyn Lionts, Liyue Fan

Faculty, Staff and Student Publications

Effective disease surveillance systems require large-scale epidemiological data to improve health outcomes and quality of care for the general population. As data may be limited within a single site, multi-site data (e.g., from a number of local/regional health systems) need to be considered. Leveraging distributed data across multiple sites for epidemiological analysis poses significant challenges. Due to the sensitive nature of epidemiological data, it is imperative to design distributed solutions that provide strong privacy protections. Current privacy solutions often assume a central site, which is responsible for aggregating the distributed data and applying privacy protection before sharing the results (e.g., …

Single-Cell Analysis Of Chromatin Accessibility In The Adult Mouse Brain, Songpeng Zu, Yang Eric Li, Kangli Wang, Ethan J Armand, Sainath Mamde, Maria Luisa Amaral, Yuelai Wang, Andre Chu, Yang Xie, Michael Miller, Jie Xu, Zhaoning Wang, Kai Zhang, Bojing Jia, Xiaomeng Hou, Lin Lin, Qian Yang, Seoyeon Lee, Bin Li, Samantha Kuan, Hanqing Liu, Jingtian Zhou, Antonio Pinto-Duarte, Jacinta Lucero, Julia Osteen, Michael Nunn, Kimberly A Smith, Bosiljka Tasic, Zizhen Yao, Hongkui Zeng, Zihan Wang, Jingbo Shang, M Margarita Behrens, Joseph R Ecker, Allen Wang, Sebastian Preissl, Bing Ren

Faculty, Staff and Student Publications

Recent advances in single-cell technologies have led to the discovery of thousands of brain cell types; however, our understanding of the gene regulatory programs in these cell types is far from complete1-4. Here we report a comprehensive atlas of candidate cis-regulatory DNA elements (cCREs) in the adult mouse brain, generated by analysing chromatin accessibility in 2.3 million individual brain cells from 117 anatomical dissections. The atlas includes approximately 1 million cCREs and their chromatin accessibility across 1,482 distinct brain cell populations, adding over 446,000 cCREs to the most recent such annotation in the mouse genome. The mouse brain cCREs are …

Brain-Wide Correspondence Of Neuronal Epigenomics And Distant Projections, Jingtian Zhou, Zhuzhu Zhang, May Wu, Hanqing Liu, Yan Pang, Anna Bartlett, Zihao Peng, Wubin Ding, Angeline Rivkin, Will N Lagos, Elora Williams, Cheng-Ta Lee, Paula Assakura Miyazaki, Andrew Aldridge, Qiurui Zeng, J L Angelo Salinda, Naomi Claffey, Michelle Liem, Conor Fitzpatrick, Lara Boggeman, Zizhen Yao, Kimberly A Smith, Bosiljka Tasic, Jordan Altshul, Mia A Kenworthy, Cynthia Valadon, Joseph R Nery, Rosa G Castanon, Neelakshi S Patne, Minh Vu, Mohammad Rashid, Matthew Jacobs, Tony Ito, Julia Osteen, Nora Emerson, Jasper Lee, Silvia Cho, Jon Rink, Hsiang-Hsuan Huang, António Pinto-Duartec, Bertha Dominguez, Jared B Smith, Carolyn O'Connor, Hongkui Zeng, Shengbo Chen, Kuo-Fen Lee, Eran A Mukamel, Xin Jin, M Margarita Behrens, Joseph R Ecker, Edward M Callaway

Faculty, Staff and Student Publications

Single-cell analyses parse the brain’s billions of neurons into thousands of ‘cell-type’ clusters residing in different brain structures1. Many cell types mediate their functions through targeted long-distance projections allowing interactions between specific cell types. Here we used epi-retro-seq2 to link single-cell epigenomes and cell types to long-distance projections for 33,034 neurons dissected from 32 different regions projecting to 24 different targets (225 source-to-target combinations) across the whole mouse brain. We highlight uses of these data for interrogating principles relating projection types to transcriptomics and epigenomics, and for addressing hypotheses about cell types and connections related to genetics. …

Lessons For The Next Generation Of Scientists From The Second Annual Arthur And Sandra Irving Cancer Immunology Symposium, Christopher Alvarez-Breckenridge, Kristin G Anderson, Ana Luisa Correia, Shadmehr Demehri, Huy Q Dinh, Karen Olivia Dixon, Gavin P Dunn, Laura Evgin, Jeremy Goc, Zinaida Good, Nir Hacohen, Patrick Han, Pavel Hanč, John Hickey, Kelly Kersten, Beiyun C Liu, Aitziber Buque, Yuxuan 'Phoenix' Miao, J Justin Milner, Yuri Pritykin, Ferdinando Pucci, Nicole E Scharping, Lisa Sudmeier, Yufei Wang, Andreas Wieland, Michelle M Williams

Faculty, Staff and Student Publications

The Arthur and Sandra Irving Cancer Immunology Symposium has been created as a platform for established cancer immunologists to mentor trainees and young investigators as they launch their research career in the field. By sharing their different paths to success, the senior faculty mentors provide an invaluable resource to support the development of the next generation of leaders in the cancer immunology community. This Commentary describes some of the key topics that were discussed during the 2022 symposium: scientific and career trajectory, leadership, mentoring, collaborations, and publishing. For each of these topics, established investigators discussed the elements that facilitate success …

An Ace2 Decamer Viral Trap As A Durable Intervention Solution For Current And Future Sars-Cov, Hailong Guo, Bomsoo Cho, Paul R Hinton, Sijia He, Yongjun Yu, Ashwin Kumar Ramesh, Jwala Priyadarsini Sivaccumar, Zhiqiang Ku, Kristen Campo, Sarah Holland, Sameer Sachdeva, Christopher Mensch, Mohamed Dawod, Annalis Whitaker, Philip Eisenhauer, Allison Falcone, Rebekah Honce, Jason W Botten, Stephen F Carroll, Bruce A Keyt, Andrew W Womack, William R Strohl, Kai Xu, Ningyan Zhang, Zhiqiang An, Sha Ha, John W Shiver, Tong-Ming Fu

Faculty, Staff and Student Publications

The capacity of SARS-CoV-2 to evolve poses challenges to conventional prevention and treatment options such as vaccination and monoclonal antibodies, as they rely on viral receptor binding domain (RBD) sequences from previous strains. Additionally, animal CoVs, especially those of the SARS family, are now appreciated as a constant pandemic threat. We present here a new antiviral approach featuring inhalation delivery of a recombinant viral trap composed of ten copies of angiotensin-converting enzyme 2 (ACE2) fused to the IgM Fc. This ACE2 decamer viral trap is designed to inhibit SARS-CoV-2 entry function, regardless of viral RBD sequence variations as shown by …

Enhanced Tp53 Reactivation Disrupts Myc Transcriptional Program And Overcomes Venetoclax Resistance In Acute Myeloid Leukemias, Yuki Nishida, Jo Ishizawa, Edward Ayoub, Rafael Heinz Montoya, Lauren B Ostermann, Muharrem Muftuoglu, Vivian R Ruvolo, Tallie Patsilevas, Darah A Scruggs, Shayaun Khazaei, Po Yee Mak, Wenjing Tao, Bing Z Carter, Steffen Boettcher, Benjamin L Ebert, Naval G Daver, Marina Konopleva, Takahiko Seki, Kensuke Kojima, Michael Andreeff

Faculty, Staff and Student Publications

The tumor suppressor TP53 is frequently inactivated in a mutation-independent manner in cancers and is reactivated by inhibiting its negative regulators. We here cotarget MDM2 and the nuclear exporter XPO1 to maximize transcriptional activity of p53. MDM2/XPO1 inhibition accumulated nuclear p53 and elicited a 25- to 60-fold increase of its transcriptional targets. TP53 regulates MYC, and MDM2/XPO1 inhibition disrupted the c-MYC-regulated transcriptome, resulting in the synergistic induction of apoptosis in acute myeloid leukemia (AML). Unexpectedly, venetoclax-resistant AMLs express high levels of c-MYC and are vulnerable to MDM2/XPO1 inhibition in vivo. However, AML cells persisting after MDM2/XPO1 inhibition exhibit a …

Support, Networks, And Relationships: Findings From A Mixed-Methods Evaluation Of A Mentorship Programme For Early Career Women Researchers In Sexual And Reproductive Health And Rights, Muhammad Asim, Peter Muriuki Gatheru, Joy J. Chebet, Mehr G. Shah, Anna Thorson, Vanessa Brizuela

Community Health Sciences

Low research output among women researchers in health research has been linked to inadequate mentorship opportunities for early career women researchers and particularly in sexual and reproductive health and rights (SRHR) field. Mentorship has been recognized as a contributor to strengthening research capacity and as beneficial for both mentors and mentees. Women researchers oftentimes experience negative impacts of organizational and structural gender inequities related to formal and informal mentoring. In 2020, the UNDP/UNFPA/UNICEF/WHO/World Bank Special Programme of Research, Development and Research Training in Human Reproduction at WHO launched a mentorship programme for early career SRHR women researchers from low- and …

Drone Swarms In Adversarial Environment, Bhavana Sai Yadav Akula

Theses

Drones are unmanned aerial vehicles (UAVs) operated remotely with the help of cameras, GPS, and on-device SD cards. These are used for many applications including civilian as well as military. On the other hand, drone swarms are a fleet of drones that work together to achieve a special goal through swarm intelligence approaches. These provide a lot of advantages such as better coverage, accuracy, increased safety, and improved flexibility when compared to a single drone. However, the deployment of such swarms in an adversarial environment poses significant challenges. This work provides an overview of the current state of research on …

A Socio-Hydrological Assessment Of Illinois Levee Systems, Nicholas Keller

Theses

Recent inspections conducted on levee safety in the U.S. that participate in the United States Army Corps of Engineers (USACE) Levee Safety Program under Public-Law 84-99 have shown that the overwhelming majority (>95%) of these levees have at least some deficiency associated with them, and many being identified as having an unacceptable safety rating (≈30%). In the U.S., many levees were constructed using funding from the federal government, but the responsibility of operation and maintenance of the levees were turned over to local government bodies. Given the local funding of levee maintenance, the socioeconomic characteristics of these levee-protected communities …

Fostering Resilience In Nurse Practitioner Practice: Development And Validation Of A Guide, Robyn L. Heiser

Doctoral Scholarly Projects & Capstones

Nurse practitioners (NPs) are a significant part of the U.S. healthcare system providing high quality, safe, consistent care often in low access and rural settings. Nurse practitioners are increasing in demand due to a growing physician shortage and a changing patient demographic of aging patients and patients with increasing complex, chronic illnesses. Nurse practitioners experience chronic work stress from layered demands of their work and role. This challenges their resilience and, if not appropriately managed, could lead to negative consequences for patient outcomes, the well-being of the NP, and overall healthcare system. Applying interventions that could effectively and efficiently foster …

Optimal Route Planning Using Computer Simulation In The Context Of Distributor-To-Consumer Supply Network, Miriam Aguilera Nava

Open Access Theses & Dissertations

Effective route planning for distribution centers is a multifaceted challenge crucial tobusiness success, as it directly impacts time and cost savings while enhancing overall delivery efficiency. Oversight of proper planning can lead to problems and inefficiencies in the distribution process. Hence, this work undertakes a comparative experiment of two delivery strategies in a distributor-to-consumer supply network. While the first strategy investigates the delivery process by zip area, the second strategy considers the clustering of the areas for more streamlined delivery fulfillment. The effectiveness of the delivery process is gauged based on a set of metrics, including total distance traveled, average …

Towards A Model Of The Mapping Between English And Spanish Prosody, Jonathan Avila

Open Access Theses & Dissertations

Current speech-to-speech translation systems face challenges in effectively translating the nuances of prosody, which plays a pivotal role in conveying speaker intent and stance in dialog. This limitation restricts cross-lingual communication, especially in situations demanding deeper interpersonal understanding. To address this, this research delves into the relationships between prosody and its pragmatic functions, in English and Spanish. First, I discuss a data collection protocol in which bilingual speakers re-enact utterances from an earlier conversation in their other language, then describe an English-Spanish corpus, consisting of 3816 matched utterance pairs. Second, I describe a prosodic dissimilarity metric based on Euclidean distance …

Upcyclying Of Polyethylene Terephtalate By Addition Of Thermoplastic Elastomer, Diego Francisco Bermudez

Open Access Theses & Dissertations

Continual overconsumption of single-use plastics has generated challenges of solid waste management across the United States. Common plastic waste management solutions, such as landfill, have caused the migration of contaminants into the environment consequently affecting not only the health of wildlife, but also that of human beings. Alternative strategies for the handling of single-use plastic such as polyethylene terephthalate (PET), used in the food packaging industry, can ultimately help mitigate the noxious consequences of single-use plastics affecting entire ecosystems. This study demonstrates a potential avenue of materials upcycling by studying the effects of coupling PET with the thermoplastic elastomer styrene-ethylene-butylene-styrene …

Development Of Metaheuristic Algorithms For The Efficient Allocation Of Power Flow Control Devices, Eduardo Jose Castillo Fatule

Open Access Theses & Dissertations

No abstract provided.

Implementation Of State Of New Mexico's Manual Of Procedures Psab Supplement 12 Capital Assets By A Southern New Mexico School District In Disposal Of E-Waste In The Post Covid 19 Environment, Hector Serrato Giron

Open Access Theses & Dissertations

The COVID-19 pandemic has caused diverse social, economic, and political challenges. The particular challenge that was the focus of this study is the upsurge in electronic use and consequent upsurge in electronic waste. This was caused by the implementation of online instructional delivery processes during Covid-19 (Ermakova, 2021), the switch to home offices (Adejumo & Oluduro, 2021) and the fact that school districts have directed massive financial investments to instructional technology (Baldé & Kuehr, 2021). This trend of heightened e-waste generation is likely to continue (Jiang et al., 2021).

I used a qualitative approach to address the two research questions. …

Deep-Learning Frameworks For The Classification And Segmentation Of Alzheimer's Disease Cell Types, Md Easin Hasan

Open Access Theses & Dissertations

Neuroimaging experts in biotech industries can benefit from using cutting-edge artificial intelligence techniques for Alzheimerâ??s disease (AD) and dementia stage prediction, despite the fact that it may be difficult to anticipate the precise stage of AD. AD is one cause of dementia with very limited to no treatment available. Cell-type classification studies are essential for developing novel drugs for this lethal and common disease. Neuronal cell segmentation is the process of identifying and separating individual neurons in an image, typically in order to study their properties or analyze their organization in the nervous system. This is significant because neurological problems …

Additively Manufactured Cryogenic Engineered Heat Pipe For Lunar Ice Collection, Mahadi Hasan

Open Access Theses & Dissertations

Nowadays, one of the most talked-about subjects in space exploration is cryogenic heat pipes. These heat pipes are made in a way that allows them to operate without the need for additional power or energy. Evaporated vapor and condensed liquid can be moved by buoyancy forces or capillary forces that are subject to gravity because of the phase shifts of the working fluids moving inside. The cooling solution typically entails a phase transition from liquid to vapor, which can dissipate larger heat fluxes because of the fluid's high latent heat of vaporization. Recent developments in additive manufacturing and micro-nano structures …

The Physiological Effects Of Acute And Ramp Hypoxic Exposure During Simulated Flight Tasks, Jasmin Renee Jenkins

Open Access Theses & Dissertations

The purpose of the present study was to examine the effects of hypoxia at simulated altitudes of FiO2 15.4% (8000’ equivalent), FiO2 13.2% (12,000’ equivalent), and a Ramp Hypoxic exposure on perceived workload, cardiovascular activity, electrodermal activity, oximetry, and flight simulator performance. A total of 17 participants (10 female & 7 males; mean ± SEM, age 24.82±1.6 yrs, BMI 25.87±1.0 who were apparently healthy, asymptomatic, and physically active performed 3 flight tasks using a video game head-mounted display (HMD) flight simulator while exposed to simulated altitudes of Normoxia, FiO2 15.4%, FiO2 13.2% and a Ramp Hypoxic Exposure (breathing at 8,000 …

Interfacial Interactions Between Engineered Nanoplastics And Biological Systems: Towards An Atomic And Molecular Understanding Of Plastics- Driven Cellular Dyshomeostasis, Afroz Karim

Open Access Theses & Dissertations

The persistent presence of micro (nano) plastics in our environment has raised concerns regarding potential health risks associated with human exposure. With the extensive use of plastic materials and the widespread consumption of plastic-contaminated food items, two types of microplastics, polystyrene (PS) and polymethylmethacrylate (PMMA), have attracted increased attention. While the adverse effects of microplastics on animals and marine life have been extensively studied, their potential impact on human health remains largely unexplored. Specifically, the interaction between microplastics and biomolecules within the human body and their effect on human health have yet to be comprehensively investigated. This dissertation study focuses …

Leveraging Agile Software Methodologies Within Software Development To Introduce A Novel Educational Software Methodology, Montserrat Guadalupe Molina

Open Access Theses & Dissertations

Agile Software Development has been growing increasingly popular in the software engineering industry as a way to produce working software in a quick and people-centered manner. Agile methodologies require practitioners to have strong technical and non-technical skills, such as teamwork, project management, and communication skills. Students graduating from the software engineering discipline have been found to be lacking in these areas, leading to many difficulties faced by recent graduates as they begin their professional careers. Given that Agile Software Development is the most popular software development lifecycle currently used by practitioners in industry, it is important to expose students to …

Bitcoin's Technical Foundation And Its Potential For A Decentralized And Environmentally Friendly Future, Iqtiar Md Siddique

Open Access Theses & Dissertations

This research examines a systematic analysis of Bitcoin, employing a Coefficient of Variation (CV) approach to gauge its degree of decentralization. Bitcoin, an innovative decentralized digital currency, has received much attention for its potential to revolutionize traditional financial systems. This study employs the Coefficient of Variation (CV) to acquire insights into the wealth distribution and concentration among Bitcoin users. This research demonstrates how decentralized the network of Bitcoin is. The methodology uses real data on Bitcoin addresses and their holdings to compute the Coefficient of Variation (CV). This approach provides valuable insights into the ongoing discourse surrounding Bitcoin's decentralization, offering …

Development Of A 3d Printed Conductive Biopolymer For Cardiac Tissue Engineering, Britanny Lizeth Stark

Open Access Theses & Dissertations

Cardiovascular disease (CVD) is the leading cause of death in the US, with approximately 859,000 deaths each year. The major contributor to CVD is Acute Myocardial Infarction (AMI), which causes the death of approximately 25% of the cardiomyocytes present in the left ventricle of the heart. After AMI, the adult human heart has a very limited regenerative capacity. Moreover, the electrical propagation of the myocardium is severely disrupted, making the heart more susceptible to failure and patient death. However, current pharmacological treatments do not address the loss of cardiomyocytes and the disruption of electrical propagation in the heart. Tissue engineering …

Evolution Of Forest Bitis (Macrocerastes) In Africa: Phylogeography, Population Genetics, And Potential As A Model For Batesian Mimicry, Eugene Richard Vaughan

Open Access Theses & Dissertations

The major biogeographical patterns characterizing the diversification of widely distributed species in the African rainforest remain poorly understood because of the low number of well-sampled studies that are focused on this region. I examine the phylogeography and population genetics of two broadly sympatric sister lineages of large African forest vipers (genus Bitis, subgenus Macrocerastes), the rhinoceros viper (B. nasicornis) and the Gaboon vipers (B. gabonica and B. rhinoceros). Despite their close relationship, these two lineages have notably different patterns of divergence in time and space. A time-calibrated phylogeny with sequence data from five genes, and population structure and PCA analyses …

Characterization Of Fcc Al-Cu-Ni-Mn-Ag High Entropy Alloy, Gina Zavala Alvarado

Open Access Theses & Dissertations

The effect of Ag on the microstructure developed in Al-Cu-Ni-Mn alloy has been determined. The modified Al-Cu-Ni-Mn alloy by Ag addition shows the presence of three microconstituents consisting of phases rich in (1) Cu, (2) Ni, and (3) Ag. The foregoing alloys heated for 24 hours from 600 to 1000 °C show excellent oxidation resistance. Oxide formation and microstructural changes of the alloy have been characterized by elemental mapping and X-ray diffraction (XRD). Results show that the elements of Al and Mn preferentially oxidize while Cu and Ni provide oxidation resistance to the alloy. Hardness was taken on the alloy …

Diverse Impacts Of Commercial Ev Charging Load Infrastructure On Electric Power Grid, Antonio Avila

Open Access Theses & Dissertations

With the rising prominence of electric vehicles (EVs) in the transportation sector, this thesis delves into the critical nexus between commercial EVs, charging infrastructure, and their consequential impacts on the power grid. As commercial EVs, particularly medium and heavy-duty variants, gain traction as viable alternatives in the commercial transportation landscape, understanding the intricacies of their charging requirements becomes paramount. This thesis critically examines the technological and logistical dimensions of the charging infrastructure for supporting commercial EVs, evaluating the consequential implications on the power grid and proposing strategies for mitigation through the utilization of Distributed Energy Resources (DERs). In tandem with …

Exploring Aging Using The Selection, Optimization, And Compensation (Soc) Framework In Conjunction With Kinematic Gait And Verbal Fluency Task Analysis In Healthy Adults, Emily Ann Chavez

Open Access Theses & Dissertations

This multi-study dissertation examined dual-task performance in young and middle-aged adults using verbal fluency tasks (VFT) during different walking scenarios (baseline, performing a VFT, walking narrowly, and performing a VFT while walking narrowly) as well as the Selection, Optimization, and Compensation (SOC) model. Thirty adults (15 young adults (YA; 19-44 years) and 15 middle-aged adults (MA; 45-65 years) participated in this study. Overall, there were no significant differences between groups when data was examined in short (means of means approach) and long-form (incorporating all data), when analyzing clustering and switching strategies, gait speed variability, or dual-task costs. Overall, these three …

Viability Of Magnetic Nanoparticles For Magnetic Hyperthermia Cancer Therapy, Marcos Adrian Garcia

Open Access Theses & Dissertations

Over the last few decades magnetic nanoparticles have gained an extraordinary amount of attention in the science community. Their versatile use in many different research areas such as medicine, engineering and technology and many other areas has made them a popular subject. In this thesis, the synthesis of different systems of magnetic nanoparticles will be explored along with the potential use of the MNP's as viable candidates for Magnetic Hyperthermia Cancer Therapy. With values of over 200 emu/g for Iron-Silver magnetic nanoparticles with particle sizes ranging from 30-70nm and their heating properties under an AC magnetic field. As well of …

Secondary Administrators' Perceptions Of Truancy Decriminalization In Texas: House Bill 2398, Mae Read

Open Access Theses & Dissertations

Seven Campus Truancy Administrator's perceptions and lived experiences were investigated as they responded to mandated changes in Texas truancy policy and how effective the implemented Truancy Prevention Measures would be perceived on high school campuses located in Far West-Texas. Campus Truancy Administrators provided insight as to interpretation of policy on their individual campus; challenges associated with enforcement of truancy policy; the effectiveness of Truancy Prevention Measures; and how implementation of these measures resulted in additional changes pertaining to loss of credit; changing graduation rates; and enforcement of Truancy Prevention Measures on student groups exempted from court proceedings.The study found Campus …