Digital Commons Network^™

Genetically Engineering Gingipains For High Yield Large Scale Purification., Andrew E. Fuchs

Electronic Theses and Dissertations

Porphyromonas gingivalis is a keystone oral pathogen in the progression of periodontal disease and has been implicated in the etiology of many other systemic human diseases. P. gingivalis produces primary virulence factors termed the gingipains, a group of cysteine proteases. There is a high demand for high purity gingipains for downstream in vitro studies where P.g. is implicated, both oral and systemic. Gingipain production candidate mutant P.g. strains were generated to isolate each gingipain for large scale purification and the purified gingipains characterized. Two mutants were determined not to be viable for large scale purification of gingipains, each for different …

Investigating The Effects Of Subchronic Dietary Zinc Supplementation On Diet-Induced Metabolic Dysfunction-Associated Steatotic Liver Disease., Oluwanifemi Esther Bolatimi

Electronic Theses and Dissertations

Zinc deficiency is associated with metabolic dysfunction-associated steatotic liver disease (MASLD). Previous studies show zinc supplementation improves steatosis, but its therapeutic effects in established MASLD remain unclear. We developed a model to characterize the effects of zinc supplementation on high-fat diet (HFD) induced MASLD and hypothesized established MASLD would be attenuated. Mice were fed control diet or HFD for 12 weeks and then grouped into normal or zinc-supplemented diets for 8 additional weeks. At euthanasia, plasma and liver tissues were collected for phenotypic analysis. Twelve weeks of HFD altered glucose clearance and body composition. Eight weeks of subsequent zinc supplementation …

Automated Usability Evaluation Utilizing Log Files And Data Mining Techniques., Sima Shafaei

Electronic Theses and Dissertations

Usability evaluation is one of the essential aspects of software production. This evaluation should be done during the entire life cycle of a software application, from pre-production to production and post-production. However, the collection and evaluation of usability data can be a very challenging, time-consuming, and expensive task to be conducted manually, particularly for certain types of products and working conditions. These challenges may include the need to recruit participants fully engage and motivate them during evaluation, and factor in environmental conditions. Other challenges may include collecting data in real-world environments, especially when the users are geographically dispersed, minimizing evaluator …

A Modular Framework For Surface-Embedded Actuation And Optical Sensing In Soft Robots., Paul Bupe Jr

Electronic Theses and Dissertations

This dissertation explores the development and integration of modular technologies in soft robotics, with a focus on the OptiGap sensor system. OptiGap serves as a simple, flexible, cost-effective solution for real-time sensing of bending and deformation, validated through simulation and experimentation. Working as part of an emerging category of soft robotics called Soft, Curved, Reconfigurable, Anisotropic Mechanisms, or SCRAMs, this research also introduces the Thermally-Activated SCRAM Limb (TASL) technology, which employs shape-memory alloy (SMA) wire embedded in curved sheets for surface actuation and served as the initial inspiration for OptiGap. In addition, the EneGate system is presented as a complementary …

Scalable Solution Processing Of Niox Nanoparticles., Peter James Armstrong

Electronic Theses and Dissertations

Perovskite solar cells (PSCs) are a promising alternative to silicon-based photovoltaics. However, PSCs face several challenges due to shortcomings in their stability, module efficiency, and scaled production. Although PSCs is still a young field of research, significant attention has been given to demonstrating power conversion efficiencies that are on par with traditional silicon. With that target reached, converting the laboratory demonstration into practical materials to increase access and abundance of solar energy are among the next large targets for the field. This comes with material challenges for perovskite and their companion charge transport layers (CTLs). Among the charge transport materials …

Synthesis And Characterization Of Silver-Coated Polyester Dendrimers With Potential Antimicorbial Properties, Che’Darria Lacey

All Theses

As bacterial cell lines continue to develop resistance to conventional antibiotic medications, the threat of untreatable bacterial infections continues to grow. The limited manufacture of new pharmaceuticals further exacerbates this severe health risk. This risk has facilitated numerous investigations to counter microbial species’ antibiotic resistance. The use of silver, a well-known natural remedy, has grown significant attraction through its use in the nanoparticle form or even as silver ions.

This thesis project involves the synthesis of branched polymers that were suitably functionalized to enable the complexation of silver ions to develop antimicrobial materials. Polyester dendrimers were synthesized utilizing divergent dendronization. …

A Hybrid Cognitive Model For Machine Agents In Project And Action Teams, Joshua A. Lapso

Theses and Dissertations

High performing human teams transcend complex domain uncertainty by achieving an emergent state of shared cognition, in which knowledge is organized, represented, and distributed to team members for rapid execution. However, this requires that individuals emit perceivable qualities upon which other members can make inferences about intent. In pursuit of future human and machine team studies, this research presents a hybrid cognitive model for machine agents in fully cooperative and semi-cooperative action and project teams. The hybrid cognitive model unifies the characteristics of the shared mental model and transactive memory system. The resultant model facilitates anytime selection over the two …

Marss: Multi-Agent Reinforcement Learning For Satellite Swarms, Nicholas J. Yielding

Theses and Dissertations

Multi-agent systems and swarms in spacecraft formation flying are of ever-increasing importance in a contested space environment—use of multiple spacecraft to contribute to a cooperative mission potentially increases positive outcomes on orbit, while autonomy becomes an ever more important requirement to reduce reaction time in dynamic situations and lower the burden on space operators. This research explores difficult swarm Guidance Navigation and Control (GNC) scenarios using Deep Reinforcement Learning (DRL). DRL polices are trained to provide guidance inputs to agents in multi-agent swarm environments for completing complex, teamwork focused objectives in geosynchronous orbit. An example scenario is explored for a …

An Improved Saliency Map With Trustworthiness For Localizing Abnormalities In Medical Imaging, Nolan C. Skelly

Theses and Dissertations

Saliency maps are a widely used methodology to make deep learning models more interpretable. They provide post-hoc explanations through identification of the most pertinent areas of an input medical image. These techniques have been assessed based on 1) localization utility, 2) sensitivity to model weight randomization, 3) repeatability, and 4) reproducibility. Ten saliency map techniques will be tested, Grad, Smooth Grad, Integrated Gradients, Smooth Integrated Gradients, XRAI, Grad-CAM, Guided Backpropagation, Guided GradCAM, GradCAM++, and ScoreCAM. The neural networks used to predict and read medical information require a reliable solution to provide medical practitioners intelligible results. Using the information of two, …

Federated Active Learning For Network Intrusion Detection, Matthew D. R. Sauer

Theses and Dissertations

This thesis addresses challenges with detecting attacks on computer networks within a Federated Learning (FL) framework, when labeled instances are few. We explore the integration of active learning (AL) and semi-supervised learning (SSL). AL efficiently uses data that would otherwise be wasted or require substantial time for labeling. SSL provides capacity to train models that have a limited amount of labeled data, by utilizing additional unlabeled data that is available. We show how FL combined with AL or SSL can realize a detection system that adapts and trains quickly to new networks, reducing the total amount of data labeling needed. …

A System Theoretic Process Analysis Framework And Model Based Approach For Resilient Space Architecture Design, Eric T. Sommer

Theses and Dissertations

As the capabilities provided by space-based systems offer significant contributions toward defense applications, potential adversaries stand to gain significant value in disrupting them. Therefore, the United States must pursue the development and operation of resilient space architectures, capable of delivering capabilities in the face of disruptions. To support this development, systems engineering methods require innovation to effectively ensure design of complex space architectures to meet their objectives. This thesis recommends and demonstrates a System-Theoretic Process Analysis (STPA) framework to qualitatively analyze space architectures. The analysis outputs identify design considerations, requirements, and constraints required for resilience. To enable a model-based systems …

Say What?! When Chatgpt Gets It Wrong: Examining Generative Ai, Section 230 Of The Communications Decency Act, And The Essence Of Creativity, Jason Davidson, Hilary G. Buttrick

Richmond Journal of Law & Technology

Since its November 2022 release, OpenAI’s large language model (“LLM”), ChatGPT, has become a household name, outscoring medical students on clinical exams, passing the bar, and polarizing academia. The wildly popular generative AI tool reached 100 million users in just sixtyone days, surpassing tech giants including Netflix, Instagram, and TikTok in reaching that milestone. ChatGPT’s robust performance in real-world applications has demonstrated its disruptive capabilities, triggering both societal excitement and concern. The advantages of this species of AI are seemingly endless: content creators can generate ideas, businesses can automate document creation, programmers can generate code, and much more.

The Development Of A Mobile Ad-Hoc Network Testbed: Modular Implementation Of Ad-Hoc On-Demand Distance Vector Routing, Gage Gailbreath, Andre Koka, Mohammed Gharib, Fatemeh Afghah

Honors College Theses

In communication systems, a Mobile Ad-Hoc Network (MANET) is a communication topology that has no central infrastructure, in contrast to more common network topologies such as Wi-fi or cellular towers. MANETs are of particular interest in the field as Internet-of-Things (IOT) applications and the push towards 6th-generation (6G) communications continues. MANET networks provide communication access to all nodes within the network using peer-to-peer communications, requiring extensive maintenance and updating of routes within the network as nodes move around. Routing protocols must be designed and used for these networks, and are typically complex algorithms that are difficult to implement on hardware. …

Examining The Effects Of Mental Health Barriers On Black Undergraduate Students Attending Predominantly White Institutions, Patrice N. Storey

Graduate Theses and Dissertations

Black college students are experiencing mental health issues at an alarming rate. There is, however, a reluctance among Black college students to seek assistance. There is a stigma surrounding mental health. An individual with a mental illness is often stereotyped as weak, uncontrollable, or paralyzed by demons. Historical incidents such as slavery, the Tuskegee Experiment, Jim Crow, and the forced sterilization of Black women have led the Black community to distrust the medical system. The stigma embedded in the Black community is passed down from generation to generation. This literature review examines existing research on Black students and their perception …

Quantification Of Dye Degradation On Titanium Dioxide-Based Membranes, Elizabeth Carol Wood

Graduate Theses and Dissertations

The purpose of this study was to examine the dye degradation on the titanium dioxide (TiO2)-based membranes. While many studies have shown photocatalytic degradation of dye on TiO2 in solution, few studies have been reported on the solid TiO2 substrate. In this work, a new method is developed to quantify the dye degradation on TiO2-based membranes. A hydrothermal method is used to synthesize the photocatalytic TiO2 nanofibers; vacuum filtration is applied to fabricate a self-assembled membrane. Silver is incorporated into the nanofibers through in situ reduction before vacuum filtration to fabricate Ag/TiO2 membrane in an attempt to red shift the …

Shallow Groundwater Distributions And Changes With Sea Level Rise In Seattle, Washington, Taylor L. Watson

Graduate Theses and Dissertations

Sea level rise (SLR) associated hazards, such as coastal flooding and erosion, are pressing issues of great concern for coastal communities across the globe. Shallow and emergent groundwater can contribute to groundwater hazards such as flooding, landslides, or damage to shallow infrastructure. This study investigates the shallow groundwater within Seattle, Washington, and projects how it will respond to SLR using interpolation and numerical groundwater modeling methods. Then, a geologic modeling method is presented as a preliminary investigation of heterogeneity and anisotropy within the aquifer system. In the results for the present-day interpolation, ~6 % of the coastal buffer –the total …

Geochemistry Of Metal-Rich Black Shales And Their Genetic Relationship To Mississippi Valley-Type Pb-Zn Ore Deposits, Midcontinent U.S., Noah S. Morris

Graduate Theses and Dissertations

The formation of Mississippi Valley-type (MVT) lead-zinc (Pb-Zn) ores is complex, involving an interaction of tectonic compression, hydrothermal fluid migration, and geochemical processes within the subsurface. Fundamental questions regarding this interaction remain unclear, including the source(s) of the Pb and Zn ions that make up the ores. A genetic link between black shales and MVT ores has been thought to exist because of (1) the presence of mature hydrocarbon and brine fluid inclusions within many MVT ores, (2) the association of the hydrothermal fluids that are linked to MVT ore formation with organic compounds and oil-like droplets, and (3) the …

Ism-Band Energy Harvesting Wireless Sensor Node, Fnu Naveed

Graduate Theses and Dissertations

In recent years, the interest in remote wireless sensor networks has grown significantly, particularly with the rapid advancements in Internet of Things (IoT) technology. These networks find diverse applications, from inventory tracking to environmental monitoring. In remote areas where grid access is unavailable, wireless sensors are commonly powered by batteries, which imposes a constraint on their lifespan. However, with the emergence of wireless energy harvesting technologies, there is a transformative potential in addressing the power challenges faced by these sensors. By harnessing energy from the surrounding environment, such as solar, thermal, vibrational, or RF sources, these sensors can potentially operate …

Characterization Of Human Fibroblast Growth Factor 2 Variant To Determine Effects On Structure, Stability, And Cell Proliferation, Ryan Layes

Graduate Theses and Dissertations

Fibroblast growth factors (FGFs) are a family of cell signaling proteins conserved across multiple species. Each individual FGF elicits different cellular functions including, but not limited to, proliferation, migration, differentiation, angiogenesis, and wound healing. One of the most studied members, fibroblast growth factor 2 (FGF2), has demonstrated substantial wound healing capacity in a wide range of tissues including skeletal, muscular, neural, respiratory, epithelial, and cardiovascular. This ability makes FGF2 a potential therapeutic for a wide range of conditions and injuries. However, due to a short half-life at room temperature, therapeutic use of FGF2 is limited. It has been demonstrated that …

Data-Centric Image Super-Resolution In Magnetic Resonance Imaging: Challenges And Opportunities, Mamata Shrestha

Graduate Theses and Dissertations

Super-resolution has emerged as a crucial research topic in the field of Magnetic Resonance Imaging (MRI) where it plays an important role in understanding and analysis of complex, qualitative, and quantitative characteristics of tissues at high resolutions. Deep learning techniques have been successful in achieving state-of-the-art results for super-resolution. These deep learning-based methods heavily rely on a substantial amount of data. Additionally, they require a pair of low-resolution and high-resolution images for supervised training which is often unavailable. Particularly in MRI super-resolution, it is often impossible to have low-resolution and high-resolution training image pairs. To overcome this, existing methods for …

Influence Of Thermal Gradient On Mechanical Properties In Fused Deposition Modelling (Fdm) Additive Manufacturing, Cori K. Yancy

All Theses

Fused Deposition Modeling (FDM) is a technique that constructs functional parts by extruding thermoplastic filaments layer by layer. The interplay between thermal dynamics and their subsequent effects on mechanical properties remains a field necessitating further exploration. This study introduced ongoing research focused on unraveling the connection between the thermal gradients in the FDM printing process and the resulting mechanical attributes. The primary objective was to increase quality and functionality of 3D printed components. In pursuit of this objective, a series of carefully planned experiments were devised to systematically vary FDM parameters, including print speed, layer thickness, and nozzle temperature. Through …

Structural Health Monitoring: The Use Of Acoustic Emission To Optimize The Fdm Additive Manufacturing Process, Ethan Phillips

All Theses

Fused Deposition Modeling (FDM) has gained widespread popularity as an affordable, versatile, and user-friendly additive manufacturing technique. However, ensuring consistent and high-quality prints remains a significant challenge. This study investigated the potential use of Nondestructive Evaluation (NDE) in the form of Acoustic Emission (AE) to optimize the FDM 3D printing process, including filament defects and the selection of print parameters. AE monitoring involves the detection and analysis of acoustic waves generated during the printing post-process, providing valuable insights into the dynamic behavior of a specimen with respect to the selected print parameters and integrity of the system. Specific acoustic patterns …

Multi-Layer Evolution Of Acoustic-Gravity Waves And Ionospheric Disturbances Over The United States After The 2022 Hunga Tonga Volcano Eruption, P. A. Inchin, A. Bhatt, S. A. Cummer, S. D. Eckermann, B. J. Harding, J. Ma, J. J. Makela, J B. Snively

Publications

e Hunga-Tonga Hunga-Ha'apai volcano underwent a series of large-magnitude eruptions that generated in the atmosphere. We investigate the spatial and temporal evolutions of fluctuations driven by atmospheric acoustic-gravity waves (AGWs) and, in particular, the Lamb wave modes in high spatial resolution data sets measured over the Continental United States (CONUS), complemented with data over the Americas and the Pacific. Along with >800 barometer sites, tropospheric observations, and Total Electron Content data from >3,000 receivers, we report detections of volcano-induced AGWs in mesopause and ionosphere-thermosphere airglow imagery and Fabry-Perot interferometry. We also report unique AGW signatures in the ionospheric D-region, measured …

Design Of Human Serum Albumin And Adenovirus Conjugation Via Catcher/Tag Molecular Glue, Peijie Zhao

McKelvey School of Engineering Graduate Student Theses & Dissertations

Adenovirus (Ad) has been the ideal cargo delivery mechanism, and its moderate immunological response makes it ideal for in vivo gene therapies since its discovery in 1953. However, the robust immunogenicity of the Ad capsid and low vaccine absorption via mucous membranes and epithelium put a limit on the process of developing intranasal vaccines. Efforts are being made to enhance the effectiveness of Ad vectors and numerous studies have demonstrated the remarkable capacity of human serum albumin (HSA) to extend plasma half-life and facilitate targeted intranasal delivery. In this study, we devised an innovative method for employing the Catcher/Tag molecular …

Improving And Integrating Quantitative Single Cell Spatial Biology For Theranostic Medicine, Peng Lu

McKelvey School of Engineering Graduate Student Theses & Dissertations

Disease states are the result of a complex interplay of many different cell types interacting in close proximity in the context of often heterogeneous tissues. Alpha particles are drawing intense research and clinical interest because of their potent cytotoxic effects and their short path lengths. Analyzing the dose distribution and tissue micro-environment for alpha therapy plays a key role in predicting the efficacy of this targeted radiotherapy. However, to date there have been no direct on-tissue analytical methods for alpha dose distributions and the corresponding tissue microenvironments.

Therefore, we have developed a pipeline to overcome this limitation by utilizing quantitative …

Illinois State University, One Hundred And Sixty-Fourth Annual Commencement, December 2023, Illinois State University

Commencement Programs

Illinois State University
One Hundred and Sixty-Fourth Annual Commencement
December 2023

Randomized Phase Ii Trial Of Dendritic Cell/Myeloma Fusion Vaccine With Lenalidomide Maintenance After Upfront Autologous Hematopoietic Cell Transplantation For Multiple Myeloma: Bmt Ctn 1401., David J Chung, Nina Shah, Juan Wu, Brent Logan, Lina Bisharat, Natalie Callander, Giulia Cheloni, Kenneth Anderson, Thinle Chodon, Binod Dhakal, Steve Devine, Poorvi Somaiya Dutt, Yvonne Efebera, Nancy Geller, Haider Ghiasuddin, Peiman Hematti, Leona Holmberg, Alan Howard, Bryon Johnson, Dimitra Karagkouni, Hillard M Lazarus, Ehsan Malek, Philip Mccarthy, David Mckenna, Adam Mendizabal, Ajay Nooka, Nikhil Munshi, Lynn O'Donnell, Aaron P Rapoport, Jane Reese, Jacalyn Rosenblatt, Robert Soiffer, Dina Stroopinsky, Lynne Uhl, Ioannis S Vlachos, Edmund K Waller, James W Young, Marcelo C Pasquini, David Avigan

Oncology Articles

PURPOSE: Vaccination with dendritic cell (DC)/multiple myeloma (MM) fusions has been shown to induce the expansion of circulating multiple myeloma-reactive lymphocytes and consolidation of clinical response following autologous hematopoietic cell transplant (auto-HCT).

PATIENTS AND METHODS: In this randomized phase II trial (NCT02728102), we assessed the effect of DC/MM fusion vaccination, GM-CSF, and lenalidomide maintenance as compared with control arms of GM-CSF and lenalidomide or lenalidomide maintenance alone on clinical response rates and induction of multiple myeloma-specific immunity at 1-year posttransplant.

RESULTS: The study enrolled 203 patients, with 140 randomized posttransplantation. Vaccine production was successful in 63 of 68 patients. At …

Nurse Care Management For Opioid Use Disorder Treatment: The Proud Cluster Randomized Clinical Trial, Paige D Wartko, Jennifer F Bobb, Denise M Boudreau, Abigail G Matthews, Jennifer Mccormack, Amy K Lee, Hongxiang Qiu, Onchee Yu, Noorie Hyun, Abisola E Idu, Cynthia I Campbell, Andrew J Saxon, David S Liu, Andrea Altschuler, Jeffrey H Samet, Colleen T Labelle, Mohammad Zare-Mehrjerdi, Angela L Stotts, Jordan M Braciszewski, Mark T Murphy, Douglas Dryden, Julia H Arnsten, Chinazo O Cunningham, Viviana E Horigian, José Szapocznik, Joseph E Glass, Ryan M Caldeiro, Rebecca C Phillips, Mary Shea, Gavin Bart, Robert P Schwartz, Jennifer Mcneely, Jane M Liebschutz, Judith I Tsui, Joseph O Merrill, Gwen T Lapham, Megan Addis, Katharine A Bradley, Proud Trial Collaborators, Megan M Ghiroli, Leah K Hamilton, Yong Hu, Jennifer S Lahue, Amy M Loree, Sean M Murphy, Thomas F Northrup, Dikla Shmueli-Blumberg, Angela J Silva, Zoe M Weinstein, Mark Tinfook Wong, Rachael P Burganowski

Faculty, Staff and Student Publications

Importance: Few primary care (PC) practices treat patients with medications for opioid use disorder (OUD) despite availability of effective treatments.

Objective: To assess whether implementation of the Massachusetts model of nurse care management for OUD in PC increases OUD treatment with buprenorphine or extended-release injectable naltrexone and secondarily decreases acute care utilization.

Design, setting, and participants: The Primary Care Opioid Use Disorders Treatment (PROUD) trial was a mixed-methods, implementation-effectiveness cluster randomized clinical trial conducted in 6 diverse health systems across 5 US states (New York, Florida, Michigan, Texas, and Washington). Two PC clinics in each system were randomized to intervention …

Biomechanical Evaluation Of Mitral Valve Repair: Virtual Chordal Transposition To Restore Anterior Leaflet Prolapse, Soohwan Jeong, Seong-Min Kim, Woojae Hong, Minsung Ko, David D Mcpherson, Hyunggun Kim

Faculty, Staff and Student Publications

Background: Surgical management of an anterior leaflet prolapse remains comparatively challenging and has led to the lack of any firmly established standard repair techniques, as seen in cases of posterior leaflet prolapse. Chordal transposition repair is widely acknowledged as a remarkably durable technique that utilizes the patient's native chordae. This study aims to evaluate and predict the biomechanical and functional characteristics of a normal mitral valve (MV) model and a pathological MV model featuring anterior ruptured mitral chordae tendineae (RMCT), and to assess the effectiveness of the chordal transposition repair in the pathological MV model.

Methods: There are four stages …

Identifying The Disproportionate Impacts Of Hurricane Harvey, Hurricane Katrina, And Covid-19 On African Americans: A Pandemic Within A Pandemic, Daniella Plaza

Dissertations (2016-Present)

African Americans have endured a disproportionately high amount of exposure to natural and man-made calamities. The racial inequities against African Americans following the hurricanes Katrina, Harvey, and the Coronavirus were examined in this study. Hurricane Katrina and Hurricane Harvey took place in two different states, but the low-income vulnerable populations encountered the same outcome which manifested as deteriorating health, lack of rapid assistance from the government, and, the exacerbation of pre-existing conditions. Literature reviews have uncovered political apathy and systemic racism. These people have lost faith in the government as evidenced by Hurricane Katrina, Hurricane Harvey, and COVID-19. The results …