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Bridging Cybersecurity Practice And Law: A Hands-On, Scenario-Based Curriculum Using The Nice Framework To Foster Skill Development, Colman Mcguan, Aadithyan Vijaya Raghavan, Komala M. Mandapati, Chansu Yu, Brian Ray, Debbie Jackson, Sathish Kumar Dec 2025

Bridging Cybersecurity Practice And Law: A Hands-On, Scenario-Based Curriculum Using The Nice Framework To Foster Skill Development, Colman Mcguan, Aadithyan Vijaya Raghavan, Komala M. Mandapati, Chansu Yu, Brian Ray, Debbie Jackson, Sathish Kumar

Electrical and Computer Engineering Faculty Publications

In an increasingly interconnected world, cybersecurity professionals play a pivotal role in safeguarding organizations from cyber threats. To secure their cyberspace, organizations are forced to adopt a cybersecurity framework such as the NIST National Initiative for Cybersecurity Education Workforce Framework for Cybersecurity (NICE Framework). Although these frameworks are a good starting point for businesses and offer critical information to identify, prevent, and respond to cyber incidents, they can be difficult to navigate and implement, particularly for small-medium businesses (SMBs). To help overcome this issue, this paper identifies the most frequent attack vectors to SMBs (Objective 1) and proposes a practical …


Context Dependent Regulation Of Dicer Function During Oocyte-To-Embryo Transition, Nick A. Newkirk Dec 2025

Context Dependent Regulation Of Dicer Function During Oocyte-To-Embryo Transition, Nick A. Newkirk

Dissertations and Theses (Open Access)

Over two decades ago, Dicer was discovered as the primary enzyme responsible for the generation of small RNAs known as the microRNAs (miRNAs) and short interfering RNAs (siRNAs). Spatiotemporal regulation of Dicer-dependent small-RNA biogenesis affects many aspects of biology, including aging, disease progression, fertility, and cancer. Interestingly, a distinct population of Dicer-dependent small RNAs, known as the endogenous siRNAs (endo-siRNAs), are uniquely abundant in the germ cells and gametes, and are critical for normal fertility. Despite their function regulating new life, the mechanisms controlling their biogenesis and subsequent germ cell functions remain poorly defined. Work to parse these mechanisms in …


Functional Analysis Of Ftsa-Mediated Recruitment Of Ftsk In Escherichia Coli, Abbigale Perkins Dec 2025

Functional Analysis Of Ftsa-Mediated Recruitment Of Ftsk In Escherichia Coli, Abbigale Perkins

Dissertations and Theses (Open Access)

Bacterial cytokinesis requires precise coordination between cytoplasmic and periplasmic components of the divisome to ensure that membrane constriction and chromosome segregation occur in synchrony. The actin-like protein FtsA plays a central role in this process by tethering FtsZ to the membrane and recruiting downstream division proteins. Despite its essential function, the mechanisms by which FtsA coordinates divisome activation and the recruitment of late-acting proteins remain incompletely defined. To address this, the role of FtsA in activating septal synthesis and recruiting the DNA translocase FtsK was examined using mutational analysis in Escherichia coli.

The FtsAM96E R153D variant, which has …


Orthogonal Comparison Of Nuclear And Mitochondrial Clonal Architectures In Hematologic Malignancies, Nehali Shah Dec 2025

Orthogonal Comparison Of Nuclear And Mitochondrial Clonal Architectures In Hematologic Malignancies, Nehali Shah

Dissertations and Theses (Open Access)

Acute myeloid leukemia (AML) is a hematologic malignancy characterized by accumulation of mutations that disrupt hematopoietic differentiation and promote clonal expansion. Understanding how these mutations arise and evolve is essential for improving diagnosis, prognosis, and treatment stratification. Current methods are limited by either restricted genomic coverage (targeted panels) or low throughput and high cost (in single-cell whole genome sequencing, scWGS). An emerging alternative is the use of mitochondrial DNA (mtDNA) mutations as clonal markers.

This study aims to determine whether mitochondrial-derived clonal architectures correlate with nuclear-derived clonal architectures in AML, thereby evaluating mtDNA as a scalable orthogonal tool for lineage …


Regulatory Settlement, Stare Decisis, And Loper Bright, Lisa Schultz Bressman, Kevin M. Stack Dec 2025

Regulatory Settlement, Stare Decisis, And Loper Bright, Lisa Schultz Bressman, Kevin M. Stack

Vanderbilt Law School Faculty Publications

In Loper Bright v. Raimondo, the Supreme Court adopted and deployed a particular narrative about agency action in support of overruling Chevron: Agencies reverse their own statutory interpretations “as much as [they] like[],” creating pervasive instability in the law, thereby destroying private reliance interests. Based on a study of two decades of agency regulations affirmed by the D.C. Circuit under Chevron, we show how infrequently agencies reversed their interpretive positions. Our study suggests that the Court’s regulatory “whiplash” narrative is overstated and that there is an underappreciated institutional settlement for notice-and-comment rules under Chevron. Identifying this regulatory settlement …


Metabolic Dysfunction Caused By Dietary Oxylipins And The Environmental Toxicant Pfos, Jazmine Alexis Eccles-Miller Dec 2025

Metabolic Dysfunction Caused By Dietary Oxylipins And The Environmental Toxicant Pfos, Jazmine Alexis Eccles-Miller

All Dissertations

Disruption of fat metabolism is a hallmark of metabolic disease. A liver enzyme, Cytochrome P450 2B6 (CYP2B6) is an anti-obesity enzyme that produced several omega-3 and omega-6 fatty acid metabolites that could contribute to metabolic diseases such as fatty liver disease. Two of these metabolites, 9-HODE and 9-HOTrE, may be associated with fatty liver. Mice were exposed to 9-HODE and 9-HOTrE to investigate whether they contribute to fatty liver disease under fasting conditions. A transgenic mice model expressing the human enzyme CYP2B6 was also fasted to determine if CYP2B6 leads to increased fatty liver under fasting conditions compared to a …


A Mixed Methods Study Of The Weather And Climate Science Curriculum Pilot In South Carolina K-12 Schools, 4-H, And Agricultural Education Programs, Emily B. Hood Dec 2025

A Mixed Methods Study Of The Weather And Climate Science Curriculum Pilot In South Carolina K-12 Schools, 4-H, And Agricultural Education Programs, Emily B. Hood

All Dissertations

Weather and climate science topics in K-12 education are more important now than ever due to extreme weather and climate events happening worldwide. Most U.S. science teachers included climate science in their courses, but they may not have a sufficient background in the topic which can hinder their efforts, or there is no explicit curriculum for the teacher to utilize. Research indicates that there is a need for innovation in the curriculum to help prepare children to become the next generation of leaders and address complex problems, such as issues related to weather and climate science. There are a few …


School Discipline Bylaws In Egypt: An Analysis Of The Framing Of Children’S Rights In Education (2014–2024), Noha Ekram Hassan Dec 2025

School Discipline Bylaws In Egypt: An Analysis Of The Framing Of Children’S Rights In Education (2014–2024), Noha Ekram Hassan

All Dissertations

This study investigates how Egypt’s School Discipline Bylaws (SDB), issued between 2014 and 2024, construct the language of discipline, children’s rights, and school governance. Using a Corpus-Assisted Critical Discourse Analysis (CADA) combined with interpretive Critical Discourse Analysis (CDA), the research identifies child-rights terminology within the five Bylaw versions, examines its relationship to the principles of the Convention on the Rights of the Child (CRC), and traces how these linguistic patterns change over time. The analysis also explores the discursive framing of discipline, sanctions, and agency, alongside a rubric-based evaluation of the SDB’s alignment with CRC principles of protection, participation, and …


Examining The Roles Of Embodiment And Theory Of Mind In Shaping User Perceptions Of Llm-Driven Conversational Agents, Elizabeth A. Schlesener Dec 2025

Examining The Roles Of Embodiment And Theory Of Mind In Shaping User Perceptions Of Llm-Driven Conversational Agents, Elizabeth A. Schlesener

All Dissertations

Large Language Models (LLMs) have advanced conversational agents, enabling natural, human-like interactions in domains such as education, programming, and workplace collaboration. Yet, user distrust persists over privacy, accuracy, and bias. As developers work to mitigate these issues and human-AI collaboration expands, reinforcing trust in LLM-driven systems is essential. To address this problem, this dissertation explores the role of anthropomorphic form in LLM-driven conversational agents and its impact on user perception.

According to the familiarity thesis, humans attribute human-like characteristics to nonhuman entities — a process known as anthropomorphism — to better comprehend unfamiliar phenomena, based on the assumption that they …


Ai In Consideration Of Her: Accounting For Gendered Workplace Dynamics In The Design And Evaluation Of Human-Centered Ai Integration In Everyday Workplaces, Kelsea S. Schulenberg Dec 2025

Ai In Consideration Of Her: Accounting For Gendered Workplace Dynamics In The Design And Evaluation Of Human-Centered Ai Integration In Everyday Workplaces, Kelsea S. Schulenberg

All Dissertations

Rapid advancements in the technical capabilities and availability of generative Artificial Intelligence (AI) systems, such as OpenAI's ChatGPT, have drawn widespread attention to the opportunities and challenges associated with AI integration into everyday workplaces (i.e., office-type work). Following calls for organizations to consider the ethical and workplace-specific impacts of generative AI's use before integrating it into the workplace, this dissertation addresses three critical gaps in Human-Centered Computing (HCC) and AI workplace integration research. First, this dissertation unpacks the underdeveloped links between women's representation - or lack thereof - in AI-related fields and how their experiences with gendered workplace dynamics in …


Surface Reaction And Transportation Of Radium-226 In Marine Systems, Jiusi Wang Dec 2025

Surface Reaction And Transportation Of Radium-226 In Marine Systems, Jiusi Wang

All Dissertations

Historical uses of radium (Ra)-containing self-luminous paint, such as on gauges and dials in naval ships, have led to the potential for such paint, and thus Ra, to be released into the marine environment. Radium-226 (226Ra) behavior in marine systems is not fully described in the literature, presenting a knowledge gap in the process of evaluating potential ecological impact of Ra in marine systems. This study explores various mechanisms contributing to Ra environmental transport in marine systems on a laboratory scale, including leaching, sorption, and diffusion. 226Ra leaching from watch hand dials containing radioactive luminous paint is …


Horticultural Strategies To Improve Yield And Efficiency For Controlled-Environment Cannabis Production, Michael John Alden Dec 2025

Horticultural Strategies To Improve Yield And Efficiency For Controlled-Environment Cannabis Production, Michael John Alden

All Dissertations

Controlled-environment cannabis (Cannabis sativa L.) production is a novel horticultural industry due to long-standing federal prohibitions associated with production, distribution, and consumption of this species. Many cannabis production methods are based on legacy knowledge and hypotheses that often contradict standard practices used in the agriculture and floriculture sectors. During this research, several horticultural practices were evaluated, including shoot number manipulation, fertilizer restriction, harvest timing, and photoperiod manipulation. Shoot number manipulation was achieved by repeatedly pinching apical shoots, which reduced reproductive shoot length (cm/shoot) and improved inflorescence:trim ratio, both of which can reduce material cost in production and labor cost …


Redefining The Script: Autonomy, Agency And Advocacy Among Black Women Educators, Racquel Plummer Dec 2025

Redefining The Script: Autonomy, Agency And Advocacy Among Black Women Educators, Racquel Plummer

All Dissertations

ABSTRACT

This qualitative study examines how Black women educators working with scripted curricula exercise autonomy, agency, and advocacy in elementary school contexts. Guided by interpretive phenomenology, the inquiry centers on lived experience and meaning-making. Data were collected through semi-structured interviews with ten educators, supplemented by reflexive journaling, member checking, and peer debriefing to enhance the trustworthiness of the findings. Analysis used iterative, thematic methods within a hermeneutic circle.

Two questions shaped the design. What beliefs and perceptions do Black women educators hold about autonomy, agency, and advocacy in scripted classrooms? How do they negotiate professional identity and role within mandates? …


The Bandwagon Model, Dean A. Williams Dec 2025

The Bandwagon Model, Dean A. Williams

All Dissertations

Professional sports teams are in the entertainment market where owners of teams invest in players with the hopes of converting their talents into wins, and in turn, added revenues for their team. To gain an understanding of the incentives that shape these team talent investment decisions, prior economists have created theoretical models of professional sports leagues that give predictions regarding the cost to acquire talent, and the winning percentage of teams from different market sizes. These models are then used to show how team talent investment decisions change once professional sports leagues implement policies such as first-year player drafts, salary …


Hydrodynamics Of Perforated Heave Plates, Muhammad Usman Dec 2025

Hydrodynamics Of Perforated Heave Plates, Muhammad Usman

All Dissertations

Ocean waves are a clean and renewable energy source that can help reduce dependence on fossil fuels and combat climate change. To capture this energy, researchers have developed devices known as wave energy converters (WECs). One common type, called a two-body point absorber WEC, uses a buoy floating on the ocean surface connected to a submerged reaction plate, known as the heave plate, which lies underwater. The relative up-and-down motion between the buoy and the heave plate, driven by ocean waves, generates usable power via a power take-off (PTO) system. Traditionally, these submerged heave plates are solid structures. However, this …


El Leñador, December 2025, El Leñador Staff Dec 2025

El Leñador, December 2025, El Leñador Staff

El Leñador Newspaper

El Leñador is an English and Spanish newspaper produced by Humboldt State University students. Our staff cover and provide news to Latinx and other diverse communities on campus and in Humboldt County. We are committed to providing relevant news and expanding the representations and stories told about people of color and other marginalized groups. Our work helps create more social, political and cultural diversity in local media.

El Leñador es un periodico en español y inglés producido por estudiantes de la Universidad Estatal de Humboldt. Nuestro personal cubre y provee noticias a la comunidad Latinx y otras comunidades diversas en …


Engage, Assess, Intervene, Evaluate: Barriers Facing The Elderly Population, Isabel Anderson, Gracie Brown, Mari Langton Dec 2025

Engage, Assess, Intervene, Evaluate: Barriers Facing The Elderly Population, Isabel Anderson, Gracie Brown, Mari Langton

Student Projects

This study examined barriers faced by older adults in Orange City, Iowa, through a mixed-methods community practice project incorporating assessment, implementation, and evaluation. During the assessment phase, researchers conducted interviews with elderly residents and professionals, gathered observational data, and facilitated community dialogue using the Nominal Group Technique. Key themes included social isolation, transportation challenges, financial strain, limited health literacy, and difficulty transitioning from independence to assisted living. A strong cultural emphasis on independence often discouraged help-seeking, while fragmented agency communication and rural limitations in specialized care further complicated service navigation. Asset mapping and power analysis highlighted community cohesion, faith-based support, …


When Environmental Provisions Acquire Primacy Over The Economic Interests – Case Comment Of The Preliminary Ruling In Case C-626/22 C.Z. And Others V Ilva, Katarzyna Błaszczak, Michał Krzaczek Dec 2025

When Environmental Provisions Acquire Primacy Over The Economic Interests – Case Comment Of The Preliminary Ruling In Case C-626/22 C.Z. And Others V Ilva, Katarzyna Błaszczak, Michał Krzaczek

Yearbook of Antitrust and Regulatory Studies

This case note analyses the Court of Justice of the European Union’s (CJEU) preliminary ruling in case C-626/22 (C. Z. and Others v Ilva). The Court interpreted the Industrial Emissions Directive (IED), ruling that Member States cannot derogate from minimum EU environmental requirements. The ruling established that environmental permits must consider all scientifically known harmful substances that could be emitted by installations. The Court also ruled that national regulations permitting repeated extensions to deadlines for installation operations, despite the identification of serious environmental and health risks, were incompatible with the IED. The CJEU’s interpretation confirms the primacy of environmental and …


Contextual Stress Profiles And Trajectories Of Internalizing Symptoms Among Adolescents In Mexican Immigrant Families, Su Yeong Kim, Wen Wen, Kiera M. Coulter, Wei Xiang Sim, Tianlu Zhang, Jingyi Shen, Yang Hou, Yishan Shen, Ka I. Ip Dec 2025

Contextual Stress Profiles And Trajectories Of Internalizing Symptoms Among Adolescents In Mexican Immigrant Families, Su Yeong Kim, Wen Wen, Kiera M. Coulter, Wei Xiang Sim, Tianlu Zhang, Jingyi Shen, Yang Hou, Yishan Shen, Ka I. Ip

Research Collection School of Social Sciences

Contextual stress experiences in early adolescents may predict distinct trajectories of depressive symptoms and anxiety across the course of adolescence. Data from 604 Mexican-origin adolescents collected from 2012 to 2020 (54% female; Mage = 12.92, SD = 0.92) showed that youth with high stress levels in multiple domains (Broadly Stressed) and those with high stress in language brokering domain only (Language Brokering Stressed) had similar levels of internalizing symptoms in early adolescence, and both groups reported higher stress than youth experiencing occasional levels of stress across domains (Occasionally Environmentally Stressed). However, youth in the Language Brokering Stressed group experienced slightly …


Comparative Assessment Of Lower Limb Neuromusculoskeletal Activation And Joint Kinematics In Normal And Impaired Gait, Jack Ryan Schule Dec 2025

Comparative Assessment Of Lower Limb Neuromusculoskeletal Activation And Joint Kinematics In Normal And Impaired Gait, Jack Ryan Schule

Theses and Dissertations

This thesis evaluates whether accessible and affordable technologies can provide clinically significant, quantifiable gait analysis comparable to traditional marker-based motion capture systems. Modern high-end systems offer excellent accuracy but remain financially and logistically inaccessible to many clinicians and researchers. To address this gap, a framework was developed using markerless pose estimation using the model, MediaPipe, inertial measurement units (IMUs) processed through an Extended Kalman Filter (EKF), and surface electromyography (sEMG) to measure joint kinematics and neuromuscular activity during gait. An experiment was performed where data was collected from participants with both normal gait and pathologies including: Osgood–Schlatter disease, Plica Band …


Presidential Removal As Article I, Not Article Ii, Gary Lawson, Jed Handelsman Shugerman Dec 2025

Presidential Removal As Article I, Not Article Ii, Gary Lawson, Jed Handelsman Shugerman

Faculty Scholarship

As a matter of original public meaning, Article I’s Necessary and Proper clause is the starting point for both Congress’s power to create offices and the limits on that power.

We believe that many legal scholars have demonstrated that, as a matter of original public meaning, the term “executive power” did not imply a presidential removal power, and Article II did not imply an indefeasible (unconditional) presidential removal power. By contrast, Article I’s Necessary and Proper Clause is a basis for limiting congressional power that is more historically grounded in original public meaning and in early practice. Tenure protections and …


“Tag, You’Re It!”: Catalyzing Latino Success Through A Latino-Centered College Access Program In Boston, Ilyitch Nahiely Tábora Dec 2025

“Tag, You’Re It!”: Catalyzing Latino Success Through A Latino-Centered College Access Program In Boston, Ilyitch Nahiely Tábora

Graduate Doctoral Dissertations

This mixed-methods case study examined the impact on Latino students of a Latino-centered college access program, the Talented and Gifted (TAG) Latino Program, based at Boston’s only public university (the University of Massachusetts Boston). Centering on the experiences of predominantly—but not exclusively—Latino alumni, the study triangulated quantitative survey data from 127 participants, qualitative data from 27 “Cafecito" focus groups involving a total of 120 alumni, and content analysis of 27 documents developed collaboratively during Cafecitos. Through this approach, the study amplified alumni voices to define the TAG program’s impact on their academic, social, personal, and professional advancement, illuminating the characteristics …


Grokking Applied To Chaotic Iterates Of The Logistic Map, Felix Donkoh Dec 2025

Grokking Applied To Chaotic Iterates Of The Logistic Map, Felix Donkoh

Electronic Theses and Dissertations

This thesis investigates grokking, the delayed transition from memorization to generalization in neural networks trained on deterministic chaotic data. Using an integer–arithmetic discretization of the logistic map, yn+1 =( a yn(p − yn))/ p 2 , bounded aperiodic sequences were generated across control parameters α ranging from 3.0 to 4.0. Transformer-based models displayed characteristic grokking curves. In periodic and chaotic regimes, validation accuracy rose suddenly after long plateaus, while at the Feigenbaum boundary (α ≈ 3.57) generalization failed completely. Increasing data diversity restored learning in chaotic domains, and explicit α–conditioning enabled a single network to generalize across all regimes. A …


Between Hard And Soft Power: A Historical Analysis Of U.S. Defense Diplomacy, Shuhei Takahashi Dec 2025

Between Hard And Soft Power: A Historical Analysis Of U.S. Defense Diplomacy, Shuhei Takahashi

War, Diplomacy, and Society (MA) Theses

In recent years, countries around the world—those of Europe but also Japan, China, and Russia—have accelerated their efforts to use military assets as instruments of diplomacy. This trend has contributed to the rising prevalence of the term “defense diplomacy” in national security discourse. The United States is no exception. Its abundant military assets and its global network of bases clearly demonstrate that it is the world’s leading practitioner of defense diplomacy. Yet the United States lacks a clear strategic document outlining the concept. Moreover, because existing definitions remain theoretically ambiguous and methods for assessing the effectiveness of defense diplomacy initiatives …


Towards Trustworthy Federated Learning, Alina Basharat Dec 2025

Towards Trustworthy Federated Learning, Alina Basharat

Theses and Dissertations

Federated learning is a collaborative training model in which multiple clients optimize aglobal model by transmitting updates to a coordinating server while keeping raw data on-device, thereby reducing direct data exposure and enabling iterative global improvement. However, the iterative communication process is vulnerable to malicious attackers that either deliberately destroy the model or curious to infer raw data. Moreover, learning from multiple agents may result in unfair results. To enhance trustworthiness within this setting, we employ two-sided norm-based screening (TNBS) that removes both abnormally large and abnormally small updates, pair it with a q-fair objective to emphasize high-loss (disadvantaged) clients, …


Clinical Phenotypes Of Motor Neurone Disease In A Kenyan Hospital-Based Population, Effie Nailah Kamadi, Jasmit Shah, Juzar Hooker, Thomas Jenkins, Dilraj Sokhi Dec 2025

Clinical Phenotypes Of Motor Neurone Disease In A Kenyan Hospital-Based Population, Effie Nailah Kamadi, Jasmit Shah, Juzar Hooker, Thomas Jenkins, Dilraj Sokhi

Internal Medicine, East Africa

Background: Motor neurone disease (MND) presentation is globally heterogenous and data on the clinical phenotype in Sub-Saharan Africa (SSA) is scarce. We sought to address this by describing the profile of MND patients in a Kenyan hospital-based population.

Methods: The medical charts of all adult MND patients assessed in the facility between January 2010 and December 2023 were retrospectively reviewed. The biographical data and clinical features of these patients were captured from their electronic and manual health records and statistical analysis performed.

Results: In total, 160 patients had their data analyzed. The male to female ratio was 1.76:1. The median …


Aged 539 Graduate Internship Report- Mount Whitney High School, Matthew C. Medeiros Dec 2025

Aged 539 Graduate Internship Report- Mount Whitney High School, Matthew C. Medeiros

Agricultural Education: Graduate Internship Reports

This project reviews the Mount Whitney High School Agricultural Education Program. Through evaluation of our program's needs, there was a lack of chapter promotion outside of social media. A chapter website was created to highlight Classroom, FFA, and SAE opportunities available to students in our program.


Alexander Asboth: Hungarian Separatist And Union Civil War General, Paul T. Brueske Dec 2025

Alexander Asboth: Hungarian Separatist And Union Civil War General, Paul T. Brueske

Graduate Theses and Dissertations (2019 - present)

This thesis reveals the experiences of a Hungarian Separatist, Alexander Asboth, and how he applied those experiences in the American Civil War. It examines Asboth’s biography, his expansive view of the Union, and how he differed from many commanding officers in the Western theater. Moreover, this thesis illuminates Asboth’s military service, including his leadership of a diverse force at Barrancas, Florida, and adds to the understudied collaboration of foreign-born officers and Black soldiers in Gulf Coast Civil War history.

During the 1848 Hungarian separatist movement, Asboth served as an Army staff officer under the leader Lajos Kossuth. However, the separatists …


Feedback Strategies In The Market With Uncertainties, Mustapha Nyenye Issah Dec 2025

Feedback Strategies In The Market With Uncertainties, Mustapha Nyenye Issah

Graduate Theses and Dissertations (2019 - present)

This paper explores how established firms use strategic advertising to deter new competitors in uncertain markets. Specifically, it models a situation where market demand evolves unpredictably - captured by the CKLS stochastic process, and the incumbent firm may be either strong or weak, a fact hidden from potential entrants. For a company already in the market, advertising is not just about driving immediate sales, it is a strategic tool to project an image of strength and deter potential new competitors. On the other side, a business thinking about entering that market faces a high-stakes, irreversible decision. It will typically hold …


Improved Model For Neurodegeneration In C. Elegans, Jean-Pierre Arditi Dec 2025

Improved Model For Neurodegeneration In C. Elegans, Jean-Pierre Arditi

Graduate Theses and Dissertations (2019 - present)

During Alzheimer's disease (AD), insoluble amyloid beta (AP) peptides accumulate to form extracellular aggregates (plaques). The direct cause of neuronal dysfunction observed in AD has been broadly investigated. The amyloid hypothesis states that AP plaques are neurotoxic, but recent studies support the AP oligomer hypothesis, which states that intracellular AP (iAP) is neurotoxic. To test this hypothesis, I used CRISPR-Cas9 gene editing to generate two transgenic Caenorhabditis elegans (C. elegans) strains. I generated a strain (RSLl 11) using the rab-3 promoter to co-express GFP in neurons, which showed no gross behavioral changes but had a 50% reduced egglaying rate. RSLl …