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Debating Empire: The Yucatan Crisis And The Political Aftermath Of The Mexican-American War, Colton Matthew Warnes Dec 2025

Debating Empire: The Yucatan Crisis And The Political Aftermath Of The Mexican-American War, Colton Matthew Warnes

Theses

This study examines the impact of the Caste War of Yucatán had on the geopolitical relationship between the United States and Mexico in the aftermath of the Mexican-American War. The briefly independent Republic of Yucatán requested foreign intervention to end the Caste War. The United States debated intervention in Yucatán, while Mexico, still reeling from the loss of the Mexican-American War and dealing with internal political conflict, made steps to reincorporate Yucatán back into Mexico and to diplomatically resist American expansion. Examining the political landscape of the United States and Mexico in the spring of 1848 offers an alternate perspective …


Beyond The Mozart Effect: How Musical Experience And Emotional Response Shape Individual Differences In Spatial Reasoning, Connor Watkinson Dec 2025

Beyond The Mozart Effect: How Musical Experience And Emotional Response Shape Individual Differences In Spatial Reasoning, Connor Watkinson

Theses

Research on the Mozart effect (the short-term enhancement of spatial-temporal reasoning after music listening) has yielded inconsistent results, often due to overlooking individual differences and using limited stimuli. This study examined multiple musical conditions (popular lyrical, classical, and white noise control) while considering the moderating roles of musical experience and the mediating influence of emotional responses. Undergraduate participants (N = 162) completed spatial reasoning assessments before and after an eight-minute listening session, alongside measures of musical background, listening habits, mood, and music-evoked emotions.Analyses revealed no overall difference in spatial reasoning improvement between music and white noise conditions. However, substantial individual …


Implementing A Nurse Navigator Role In An Outpatient Procedural Setting To Improve Patient Satisfaction Scores: A Quality Improvement Project, Lashanti King Dec 2025

Implementing A Nurse Navigator Role In An Outpatient Procedural Setting To Improve Patient Satisfaction Scores: A Quality Improvement Project, Lashanti King

Doctor of Nursing Practice Projects

Much focus has been placed on patient experience since the Affordable Care Act was signed into law in 2010 due to its regulatory, financial, and business growth implications. Patient satisfaction is crucial to maintaining and growing a customer base and fulfilling the target growth established in institutional strategic plans. This project aimed to determine whether implementing a nurse navigator role to perform rounds in the preoperative and waiting areas for two months would increase patient-reported satisfaction scores regarding being informed about delays and the likelihood of recommending the facility. Due to its subjective nature, it is challenging to consistently maintain …


Best Practices For Local Law Enforcement In Counterterrorism: Enhancing Interagency Communication & Developing Modern Information Sharing Systems, Michael G. Fitterman Dec 2025

Best Practices For Local Law Enforcement In Counterterrorism: Enhancing Interagency Communication & Developing Modern Information Sharing Systems, Michael G. Fitterman

Master's Theses

Local law enforcement agencies play a critical and increasingly central role in the United States’ domestic counterterrorism architecture. While federal agencies possess sophisticated intelligence capabilities, local officers are often the first to encounter early indicators of radicalization, pre-operational planning, and suspicious behavior. Despite this frontline role, communication between local, state, and federal agencies remains fragmented, inconsistent, and sometimes informal. These gaps weaken national situational awareness and limit the ability of agencies to identify emerging threats.

This thesis examines the structural, technological and cultural challenges hat impede effective information sharing among law enforcement agencies. It outlines best practices that local departments …


Exploring The Relationship Between Job Satisfaction And Servant Leadership In Community Corrections: A Probation Officer’S Perspective, Shamira B. Gambrell Dec 2025

Exploring The Relationship Between Job Satisfaction And Servant Leadership In Community Corrections: A Probation Officer’S Perspective, Shamira B. Gambrell

Education Doctoral

Probation officers work in demanding environments marked by high caseloads, role conflict, and organizational pressures that can negatively affect job satisfaction and workforce stability. Leadership practices at the frontline supervisory level play a critical role in shaping these experiences, yet limited empirical research has examined servant leadership within community corrections. The purpose of this quantitative correlational study was to examine the relationship between probation officers’ perceptions of servant leadership demonstrated by their direct supervisors and their level of job satisfaction.

Data were collected from probation officers affiliated with departments connected to the American Probation and Parole Association using an anonymous …


An Interest-Driven Approach: An Interview With Professor Allan Leslie Combs, Laurel Waterman Phd Dec 2025

An Interest-Driven Approach: An Interview With Professor Allan Leslie Combs, Laurel Waterman Phd

Journal of Conscious Evolution

On March 17, 2024, I interviewed Professor Allan Leslie Combs about his teaching for my doctoral research project titled Teaching With Consciousness: A Narrative Inquiry Into Scholars’ Experiences Teaching About Consciousness Beyond the Brain. In January 2025, Leslie reviewed a draft of my interview write-up and emailed me, saying, “Beautiful work! And I have to say I am honored by the description you give of my own teaching philosophy.” This article draws on Leslie’s stories and applies their insights to the theory and practice of consciousness education—a field exploring perspectives on the source and nature of consciousness and their …


Deficiencies In Non-Emergency Medical Transportation For Aging Rural Residents Of Iosco County, Michigan, Richard L. Douglass, Donald Stansloski, James Mortimer, Sam Purdy, Dale Evilsizer, Scott Rice Dec 2025

Deficiencies In Non-Emergency Medical Transportation For Aging Rural Residents Of Iosco County, Michigan, Richard L. Douglass, Donald Stansloski, James Mortimer, Sam Purdy, Dale Evilsizer, Scott Rice

Michigan Journal of Public Health

Introduction: The rural aging population in Michigan struggles to access medical care often available only at significant distances due to limited health system resources and provider shortages. Access to routine care is challenged by lack of non-emergency medical transportation (NEMT). The Iosco County Commission on Aging provides home-delivered meals to eligible county residents. The Rural Health Group investigated the needs of home-delivered meal recipients (aged ≥60 years) for healthcare transportation access. Utilizing data from this investigation, we examined if medical care needs were hindered by problems with transportation in rural Iosco County, Michigan.

Methods: We conducted a cross-sectional survey with …


Fostering Dialogue, Learning, And Reflection: The Role Of Book Discussions For Public Health Professionals, Naomi E. Ervin, Belinda Aberle, Katherine Balint, Lynn Mcdaniels, Paula Tomczak Dec 2025

Fostering Dialogue, Learning, And Reflection: The Role Of Book Discussions For Public Health Professionals, Naomi E. Ervin, Belinda Aberle, Katherine Balint, Lynn Mcdaniels, Paula Tomczak

Michigan Journal of Public Health

Background: The Public Health Nursing (PHN) Section of the Michigan Public Health Association (MPHA) works to improve public health and public health nursing practice in Michigan. In 2021, the PHN Section initiated virtual book discussions to engage public health professionals in meaningful discussion about topics of critical importance to public health and public health nursing; provide continuing education contact hours; and recruit new section members. Methods: The discussions are conducted virtually four times per year. A moderator introduces each book and leads the one-hour discussion. As an added incentive to read the books, the PHN Section sends a limited number …


Data-Driven Evaluation Of Sustainable Waste-To-Energy Pathways For Intelligent Urban Systems, Izech Brian O. Edwin, King Harold A. Recto Dec 2025

Data-Driven Evaluation Of Sustainable Waste-To-Energy Pathways For Intelligent Urban Systems, Izech Brian O. Edwin, King Harold A. Recto

Electronics, Computer, and Communications Engineering Faculty Publications

The handling of municipal solid waste (MSW) in swiftly urbanizing Philippine cities poses intricate energy and governance challenges. In Baguio City, reliance on landfills has reached critical levels due to diminishing capacity, rising transport costs, and opposition to trash transfers by nearby LGUs. Although shaped by unique topographical and governance constraints, Baguio’s situation reflects issues faced by other rapidly growing Philippine cities; therefore, analyzing it offers insights for national MSW decision-making. This study applies a triple bottom line (TBL) framework to assess three management scenarios: (1) Status Quo, where all MSW is landfilled with no energy recovery; (2) Landfill with …


Itadakimasu: Recipes From Japanese American Internment Camps, Alexander K. Nishida Dec 2025

Itadakimasu: Recipes From Japanese American Internment Camps, Alexander K. Nishida

Graphic Communication

This project presents a historical narrative told through the format of a cookbook, exploring the relationship between food, memory, and resilience during the Japanese American Internment of World War II. Using primary resources such as news publications from within the camps (Topaz Times,1943), historical interviews (Herzig, 1994), and secondary resources from an archival podcast (Campu, 2021) to highlight how food served as both a means of survival and an expression of identity under confinement.

The cookbook will combine recipes inspired by documented internment-era meals with stories and reflections gathered from interviews, archival materials, and community voices. Each section will contextualize …


Sars-Cov-2 Rna-Binding Protein Suppresses Extracellular Mirna Release, Hyejin Mun, Chang Hoon Shin, Qingxuan Fei, Andrea Estefania Lopez Giraldo, Kyoung-Min Choi, Ji Won Lee, Kyungmin Kim, Kyung-Won Min, Leilei Shi, Mark T Bedford, Dong-Chan Kim, Yoo Lim Chun, Seonghyun Ryu, Dongin Kim, Jeong Ho Chang, Ryan T Westrope, Michelle Shay, Edward Nguyen, Junho K Hur, Abigail Agyenda, Nam Chul Kim, Sung-Ung Kang, Woonghee Lee, Je-Hyun Yoon Dec 2025

Sars-Cov-2 Rna-Binding Protein Suppresses Extracellular Mirna Release, Hyejin Mun, Chang Hoon Shin, Qingxuan Fei, Andrea Estefania Lopez Giraldo, Kyoung-Min Choi, Ji Won Lee, Kyungmin Kim, Kyung-Won Min, Leilei Shi, Mark T Bedford, Dong-Chan Kim, Yoo Lim Chun, Seonghyun Ryu, Dongin Kim, Jeong Ho Chang, Ryan T Westrope, Michelle Shay, Edward Nguyen, Junho K Hur, Abigail Agyenda, Nam Chul Kim, Sung-Ung Kang, Woonghee Lee, Je-Hyun Yoon

Faculty, Staff and Student Publications

SARS-CoV-2 is the betacoronavirus causing the COVID-19 pandemic. Although the SARS-CoV-2 genome and transcriptome were reported previously, the function of individual viral proteins is largely unknown. Utilizing biochemical and molecular biology methods, we identified that four SARS-CoV-2 RNA-binding proteins (RBPs) regulate the host RNA metabolism by direct interaction with mature miRNA let-7b revealed by Nuclear Magnetic Resonance spectroscopy (NMR). SARS-CoV-2 RBP Nsp9 primarily binds mature miRNA let-7b, a direct ligand of the Toll-like Receptor 7 (TLR7), one of the potential SARS-CoV-2 therapeutics. Nsp9 suppresses host gene expression possibly by promoting let-7b-mediated silencing of a cellular RNA polymerase, POLR2D. In addition, …


A Review Of Engraftment Assessments Following Fecal Microbiota Transplant, Chloe Herman, Bridget M Barker, Thais F Bartelli, Vidhi Chandra, Rosa Krajmalnik-Brown, Mary Jewell, Le Li, Chen Liao, Florencia Mcallister, Khemlal Nirmalkar, Joao B Xavier, J Gregory Caporaso Dec 2025

A Review Of Engraftment Assessments Following Fecal Microbiota Transplant, Chloe Herman, Bridget M Barker, Thais F Bartelli, Vidhi Chandra, Rosa Krajmalnik-Brown, Mary Jewell, Le Li, Chen Liao, Florencia Mcallister, Khemlal Nirmalkar, Joao B Xavier, J Gregory Caporaso

Faculty, Staff and Student Publications

Fecal Microbiota Transplant (FMT) is a treatment for recurrent Clostridium difficile infections and is being explored for other clinical applications, from alleviating digestive and neurological disorders, to restoring microbiomes impacted by cancer treatment. Quantifying the extent of engraftment following an FMT is important in understanding a recipient's response to treatment. Engraftment and clinical response need to be investigated independently to evaluate an FMT's role (or lack thereof) in achieving a clinical response. Standardized bioinformatics methodologies for quantifying engraftment extent would not only improve assessment and understanding of FMT outcomes, but also facilitate comparison of FMT results and protocols across studies. …


Clinical And Genomic Characterization Of Serratia Bloodstream Infections, Roberta Monardo, Lawrence P Park, Felicia Ruffin, Phillip Cox, An Q Dinh, Rachel E Korn, Parisa Farahani, Marco Ripa, Antonella Castagna, Blake M Hanson, Vance G Fowler, Joshua T Thaden, Stacey A Maskarinec Dec 2025

Clinical And Genomic Characterization Of Serratia Bloodstream Infections, Roberta Monardo, Lawrence P Park, Felicia Ruffin, Phillip Cox, An Q Dinh, Rachel E Korn, Parisa Farahani, Marco Ripa, Antonella Castagna, Blake M Hanson, Vance G Fowler, Joshua T Thaden, Stacey A Maskarinec

Faculty, Staff and Student Publications

Background: Previously considered a rare opportunistic pathogen, Serratia is now an emerging cause of bacteremia. We compared the risk factors and outcomes of patients with Serratia bacteremia relative to other Enterobacterales in a cohort of hospitalized patients.

Methods: Patients were prospectively enrolled from 2002-2021 at Duke University. Characteristics and outcomes were compared among bacteremic patients due to Serratia and other Enterobacterales. Regression models were used to determine features associated with outcomes. Whole genome sequencing (WGS) was performed to characterize the phylogenetic diversity of Serratia isolates associated with device infections.

Results: Of 2676 patients, 173 (6.5%) had Serratia bacteremia. Among patients …


Crux: Exploring Imperialism And Resistance Through Role-Playing Games, Devon Gronka Dec 2025

Crux: Exploring Imperialism And Resistance Through Role-Playing Games, Devon Gronka

Institute for the Humanities Master's Papers, Projects, and Capstones

This is a game about power. The game is a Role-Playing Game, or RPG. As with nearly all RPGs, the game presents the players with choices about how their characters will interact with each other and with the world around them, and these interactions are woven together to more or less form a plot. However, oftentimes in life there are no good choices available, and there is no win state outside of what we might determine for ourselves.

The game is a social experiment. While playing a game, a player takes in information in a very different manner than when …


The Silent Sound Of Drowning: Human Rights Justifications And Complex Intersectionality, Maria Grahn-Farley Dec 2025

The Silent Sound Of Drowning: Human Rights Justifications And Complex Intersectionality, Maria Grahn-Farley

Brooklyn Journal of International Law

This Article presents a new concept, “Human Rights Justifications,” which captures instances in which states invoke human rights to defend and legitimize their own actions. Human Rights Justifications stand in contrast to cases in which human rights are activated by an individual holding the state accountable for its actions. The difference between when a state and when an individual is the activating subject is that with the former, human rights risk serving the state in legitimizing its actions, instead of serving the individual as a protection from the state. This occurs because, when the state activates human rights, it also …


National Values Matter For Ai: Comparative Analysis Of Ai Regulations In The Us, The Eu, And China, Kaori (Furuya) Kawaguchi Dec 2025

National Values Matter For Ai: Comparative Analysis Of Ai Regulations In The Us, The Eu, And China, Kaori (Furuya) Kawaguchi

Brooklyn Journal of International Law

As artificial intelligence (AI) continues to evolve rapidly, governments worldwide face increasing pressure to regulate it. This Article focuses on regulatory approaches to AI in the United States, the European Union, and China, examining the underlying rationales: why have they taken these approaches? Each jurisdiction’s unique political institutions, historical preferences, and strategic priorities shape its approach to governance. In particular, the values the jurisdiction expects in technology shape its regulation. The US adopts a decentralized, innovation-friendly model rooted in reliance on market and self-regulation in industries with its strong trust in laissez-faire philosophy; the EU asserts global leadership in regulations …


Safeguarding Creditors' Interest In China's Debtor-In-Possession (Dip) Model: Enhancing Director Accountability For Effective Corporate Reorganization, Tianqi (Alicia) Ding Dec 2025

Safeguarding Creditors' Interest In China's Debtor-In-Possession (Dip) Model: Enhancing Director Accountability For Effective Corporate Reorganization, Tianqi (Alicia) Ding

Brooklyn Journal of International Law

In recent years, China has increasingly adopted the Debtor-in-Possession (DIP) model in corporate reorganization, allowing directors to retain control of the debtor’s operations during bankruptcy proceedings. From 2019 to 2025, the use of the DIP model among listed companies rose substantially, reflecting a policy preference for efficiency, continuity of management, and the perceived advantages of director familiarity with business operations. While the DIP model may improve restructuring efficiency, it also concentrates decision-making power in directors who face limited personal accountability, thereby exposing creditors to heightened risk during insolvency. China’s existing legal framework inadequately addresses this risk. The Company Law defines …


United States Counsel Being Used In Order To Serve Foreign Entities: Service On Foreign Entities And The Relationship Between Us Counsel And Foreign Companies, Lucy Gottfried Dec 2025

United States Counsel Being Used In Order To Serve Foreign Entities: Service On Foreign Entities And The Relationship Between Us Counsel And Foreign Companies, Lucy Gottfried

Brooklyn Journal of International Law

This Note examines the use of alternative service on foreign defendants’ United States counsel under Federal Rule of Civil Procedure 4(f)(3) and USCIT Rule 4(e)(3). A recent challenge in the Court of International Trade has raised the issue of whether this method of service complies with the rule that service be effectuated in a foreign country. Through surveying federal courts decisions addressing motions for alternative service on U.S. counsel, this note demonstrates that courts do not apply a uniform rule. Instead, courts balance different considerations—like due process, international comity, and litigants’ behavior—based on the underlying facts. This Note synthesizes the …


International Trade And Climate Policy: Revising The Benefit Analysis For Countervailing Duties, Michael J. Havens Dec 2025

International Trade And Climate Policy: Revising The Benefit Analysis For Countervailing Duties, Michael J. Havens

Brooklyn Journal of International Law

The United States’ Countervailing Duties Law protects domestic markets by offsetting any benefit foreign manufacturers receive from their domestic governments’ subsidy programs. The benefit analysis the Department of Commerce uses under the Countervailing Duties Law, however, is too vague. Moreover, the sole environmental exemption for otherwise countervailable subsidies is too narrow. As a result, Commerce assesses duties on an array of manufacturers burdened by foreign governments’ climate policies. The case study BGH Edelstahl v. US underscores the issue of the Countervailing Duties Law’s definition of “benefits conferred” and the limited environmental exemption. This Note compares the shortcomings of the Countervailing …


"Reeling In" The Governance Problem: How To Achieve Sustainable Fisheries On An International Scale, Louis Morais-Andrade Dec 2025

"Reeling In" The Governance Problem: How To Achieve Sustainable Fisheries On An International Scale, Louis Morais-Andrade

Brooklyn Journal of International Law

In 2025, it was estimated that over 35% of international fish stocks were overfished, risking severe depletion of high seas fisheries. In general, countries have control over fisheries within 200 nautical miles of their coastal border. Many countries have established fishery conservation laws to help prevent overfishing. Some examples of the largest domestic fishing laws are the Magnuson-Stevens Fishery Conservation and Management Act and the European Union’s Common Fishery Policy (MSA), which functions as an international policy that governs all of the United States’ fisheries and the European Union’s Common Fisheries Policy (CFP). Concurrently, the United Nations has also opened …


More Than Medals: Protecting The Olympic Brand, Olivia M. Zykoff Dec 2025

More Than Medals: Protecting The Olympic Brand, Olivia M. Zykoff

Brooklyn Journal of International Law

The enforcement of intellectual property rights is critical for brand protection. When a country is given the power to host an event as grand as the Olympic Games, it takes on immense responsibility to protect all aspects of the Games ahead. Importantly, as an international event that travels to a new host country every two years, the Olympic brand requires centralized protection, to be overseen by a leading body, the International Olympic Committee (IOC). This Note reviews the foundational protections given to the Olympic brand by the IOC and examines the country-specific treatment and protections granted to Olympic properties at …


Tapping Out Of The Wiretap: Whether Consumers Have A Reasonable Expectation Of Privacy Against Internet Tracking Technology, Ashley Faranesh Dec 2025

Tapping Out Of The Wiretap: Whether Consumers Have A Reasonable Expectation Of Privacy Against Internet Tracking Technology, Ashley Faranesh

Brooklyn Journal of Corporate, Financial & Commercial Law

The California Invasion of Privacy Act (CIPA), a statute born in an era of rotary phones and analog surveillance, is now at the heart of a legal crisis over internet tracking and privacy. Plaintiffs have increasingly weaponized the CIPA against online businesses that use standard Web-tracking tools, triggering a wave of litigation that stretches the statute far beyond its intended purpose. This Note argues that such application misreads the legislative intent behind the CIPA and risks destablizing core structures of the internet economy and financially crippling online businesses. Courts applying literal interpretations are undermining the CIPA’s purpose by conflating commonplace …


The Messenger, Vol. 52, No. 2 Dec 2025

The Messenger, Vol. 52, No. 2

The Messenger (archival - 1870-)

No abstract provided.


Decomposition Processes And Insect Indicators In Forensic Investigations, Eli Taylor Dec 2025

Decomposition Processes And Insect Indicators In Forensic Investigations, Eli Taylor

University Honors Theses

Insects play an essential biological and ecological role in human life, and their predictable relationship with decomposing remains has positioned them as a cornerstone of modern forensic science. Since the early 20th century, forensic entomology has provided critical evidence for estimating the postmortem interval (PMI), relying on patterns of colonization, species succession, and insect developmental timelines. Accurate PMI estimations are vital to medicolegal investigations, influencing the interpretation of biological evidence, guiding resource allocation, and informing determinations of cause and manner of death. However, insect activity is profoundly shaped by environmental and ecological variables, including temperature, humidity, seasonal shifts, and geographic …


Democratic Integrity Under Threat: Confronting Disinformation With Legislative And Structural Reforms, Sophia Brociner Dec 2025

Democratic Integrity Under Threat: Confronting Disinformation With Legislative And Structural Reforms, Sophia Brociner

Journal of Law and Policy

In the modern digital era, disinformation has evolved from a political strategy into a fundamental threat to democratic governance. This Note contends that the absence of comprehensive federal legislation addressing political disinformation on social media has compromised both the integrity of elections and the public’s trust in government institutions. By analyzing the disinformation campaigns that shaped the 2016, 2020, and 2024 presidential elections, this Note demonstrates how false and AI-generated content, amplified by both foreign and domestic actors, has manipulated voters, deepened political polarization, and destabilized public confidence in democratic processes. The discussion examines how rapid technological development, insufficient regulatory …


The Heteronormative Language Of Law: The Win In Bostock And The Loss For Title Ix, Negar Khalaf Dec 2025

The Heteronormative Language Of Law: The Win In Bostock And The Loss For Title Ix, Negar Khalaf

Journal of Law and Policy

The struggle for equality in education begins with language. Legal categories like “sex” and “gender identity” shape who is protected and who is left behind. When the Supreme Court decided Bostock v. Clayton County, it extended protection to LGBTQ+ workers but offered no definition robust enough to secure similar rights for students under Title IX. Subsequent efforts by the Department of Education to bridge that gap—culminating in Tennessee v. Cardona and constrained by Loper Bright Enterprises v. Raimondo—reveal the limits of agency action in a post-Chevron era. This Note calls for a legislative solution: an amended Equality Act Bill (“EAB”) …


Bridging Cybersecurity Practice And Law: A Hands-On, Scenario-Based Curriculum Using The Nice Framework To Foster Skill Development, Colman Mcguan, Aadithyan Vijaya Raghavan, Komala M. Mandapati, Chansu Yu, Brian Ray, Debbie Jackson, Sathish Kumar Dec 2025

Bridging Cybersecurity Practice And Law: A Hands-On, Scenario-Based Curriculum Using The Nice Framework To Foster Skill Development, Colman Mcguan, Aadithyan Vijaya Raghavan, Komala M. Mandapati, Chansu Yu, Brian Ray, Debbie Jackson, Sathish Kumar

Electrical and Computer Engineering Faculty Publications

In an increasingly interconnected world, cybersecurity professionals play a pivotal role in safeguarding organizations from cyber threats. To secure their cyberspace, organizations are forced to adopt a cybersecurity framework such as the NIST National Initiative for Cybersecurity Education Workforce Framework for Cybersecurity (NICE Framework). Although these frameworks are a good starting point for businesses and offer critical information to identify, prevent, and respond to cyber incidents, they can be difficult to navigate and implement, particularly for small-medium businesses (SMBs). To help overcome this issue, this paper identifies the most frequent attack vectors to SMBs (Objective 1) and proposes a practical …


Benzodioxane-Benzamides Targeting Bacterial Cell Division Protein Ftsz Potentially Disrupt Slma-Mediated Nucleoid Occlusion And Reversible Biomolecular Condensation, Marta Sobrinos-Sanguino, Inés Barros-Medina, Lorenzo Suigo, Alessia Lanzini, Ermanno Valoti, William Margolin, Valentina Straniero, Begoña Monterroso, Silvia Zorrilla Dec 2025

Benzodioxane-Benzamides Targeting Bacterial Cell Division Protein Ftsz Potentially Disrupt Slma-Mediated Nucleoid Occlusion And Reversible Biomolecular Condensation, Marta Sobrinos-Sanguino, Inés Barros-Medina, Lorenzo Suigo, Alessia Lanzini, Ermanno Valoti, William Margolin, Valentina Straniero, Begoña Monterroso, Silvia Zorrilla

Faculty, Staff and Student Publications

New strategies are urgently needed against antimicrobial resistance, a major health threat, and the different mechanisms regulating the bacterial cell division machinery offer multiple opportunities for developing novel therapeutics. FtsZ, an essential protein of this process, is targeted by multiple small molecules, and benzodioxane-benzamides (BDOBs) are among the most potent inhibitors in several bacterial species. BDOBs mechanisms are however poorly understood, particularly their impact on FtsZ's interplay with partners and ability to assemble phase-separated biomolecular condensates potentially involved in stress sensing. We show that certain BDOBs shielded FtsZ against depolymerization induced by the nucleoprotein complexes of SlmA, which inhibit Z-ring …


Context Dependent Regulation Of Dicer Function During Oocyte-To-Embryo Transition, Nick A. Newkirk Dec 2025

Context Dependent Regulation Of Dicer Function During Oocyte-To-Embryo Transition, Nick A. Newkirk

Dissertations and Theses (Open Access)

Over two decades ago, Dicer was discovered as the primary enzyme responsible for the generation of small RNAs known as the microRNAs (miRNAs) and short interfering RNAs (siRNAs). Spatiotemporal regulation of Dicer-dependent small-RNA biogenesis affects many aspects of biology, including aging, disease progression, fertility, and cancer. Interestingly, a distinct population of Dicer-dependent small RNAs, known as the endogenous siRNAs (endo-siRNAs), are uniquely abundant in the germ cells and gametes, and are critical for normal fertility. Despite their function regulating new life, the mechanisms controlling their biogenesis and subsequent germ cell functions remain poorly defined. Work to parse these mechanisms in …


Functional Analysis Of Ftsa-Mediated Recruitment Of Ftsk In Escherichia Coli, Abbigale Perkins Dec 2025

Functional Analysis Of Ftsa-Mediated Recruitment Of Ftsk In Escherichia Coli, Abbigale Perkins

Dissertations and Theses (Open Access)

Bacterial cytokinesis requires precise coordination between cytoplasmic and periplasmic components of the divisome to ensure that membrane constriction and chromosome segregation occur in synchrony. The actin-like protein FtsA plays a central role in this process by tethering FtsZ to the membrane and recruiting downstream division proteins. Despite its essential function, the mechanisms by which FtsA coordinates divisome activation and the recruitment of late-acting proteins remain incompletely defined. To address this, the role of FtsA in activating septal synthesis and recruiting the DNA translocase FtsK was examined using mutational analysis in Escherichia coli.

The FtsAM96E R153D variant, which has …