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The Overestimation Of Concentric Hypertrophy In Patients With Hfpef As Determined By 2d-Echocardiography, Mohammad F. Mathbout, Hussam Al Hennawi, Anwar Khedr, Gaurang N. Vaidya, Marcus Stoddard Jun 2022

The Overestimation Of Concentric Hypertrophy In Patients With Hfpef As Determined By 2d-Echocardiography, Mohammad F. Mathbout, Hussam Al Hennawi, Anwar Khedr, Gaurang N. Vaidya, Marcus Stoddard

Division of Internal Medicine Faculty Papers & Presentations

Background: Heart failure with preserved ejection fraction continues to pose multiple challenges in terms of accurate diagnosis, treatment, and associated morbidity. Accurate left ventricular (LV) mass calculation yields essential prognostic information relating to structural heart disease. Two-dimensional (2D) echocardiography-based calculations are solely limited to LV geometric assumptions of symmetry, whereas three-dimensional (3D) echocardiography could overcome these limitations. This study aims to compare the performance of 2D and 3D LV mass calculations. Methods: A prospective review of echocardiography findings at the University of Louisville, Kentucky, was conducted and assessed. Normal ejection fraction (EF) was defined as >=52% in males and >=54% …


Analysis Of The Governance Frameworks Of Public Universities In Tanzania: What Is Known And Needs Knowing?, Siasa Issa Mzenzi Jun 2022

Analysis Of The Governance Frameworks Of Public Universities In Tanzania: What Is Known And Needs Knowing?, Siasa Issa Mzenzi

Business Management Review

Little is known about governance frameworks of universities in developing countries. In response, this paper presents content analysis undertaken to review the governance frameworks of public universities in Tanzania, encompassing the Universities Act, 2005, the Universities (General) Regulations, 2013 and the charters of ten public universities. In order to understand the changes that have taken place, individual acts of parliament relating to public universities prior to 2005 are also reviewed. The analysis reveals that changes to universities ' governance frameworks through enactment of the Universities Act, 2005 have substantially increased both the institutional and operational autonomy of public universities. At …


Influence Of Board Attributes On Board Roles Performance In Savings And Credit Co-Operative Societies (Saccos) In Tanzania, Lilian Simon Mlay, Sylvia Shayo Temu, Lucas Mataba Jun 2022

Influence Of Board Attributes On Board Roles Performance In Savings And Credit Co-Operative Societies (Saccos) In Tanzania, Lilian Simon Mlay, Sylvia Shayo Temu, Lucas Mataba

Business Management Review

This paper reports the findings of a study that had evaluated the influence of board attributes size, gender diversity, skills and meetings on board ' s role performance in terms of strategic, resource provision and monitoring roles in SACCOS, Tanzania. Data on the boards ' roles were collected using a questionnaire survey administered to 198 SACCOS boards ' chairpersons. Data for board ' s attributes were obtained from annual audited financial reports. Data were then subjected to factor analysis and linear regression models. The results show that board meetings have a positive and significant influence on the boards ' ability …


Otonomi Daerah Dalam Masyarakat Multikultural, Bambang Supriyono Jun 2022

Otonomi Daerah Dalam Masyarakat Multikultural, Bambang Supriyono

Jurnal Konstitusi & Demokrasi

The local government system implemented in a multicultural society, especially in Indonesia, still faces a dilemma. When the government system prioritizes democratic values, there is a tendency to ignore managerial values. Observing these issues, the decentralization policy as the basis for regional autonomy will be appropriately implemented if it considers the balance between diversity and the dimensions of unity or diversity. Understanding these multicultural dimensions needs to be the basis for building relationships between the central and regional governments in administering a government system that truly means local self-governance.


Konstitusionalitas Penerapan Mekanisme Omnibus Law Dalam Pembentukan Undang-Undang Di Indonesia, Charles Simabura, M. Nurul Fajri Jun 2022

Konstitusionalitas Penerapan Mekanisme Omnibus Law Dalam Pembentukan Undang-Undang Di Indonesia, Charles Simabura, M. Nurul Fajri

Jurnal Konstitusi & Demokrasi

This paper intends to describe how the omnibus law mechanism applied in lawmaking in Indonesia. Look at the 2020 priority national legislation program, which includes four draft bills that formed using the omnibus law mechanism. The omnibus law is a new mechanism in the lawmaking process in Indonesia. Because this mechanism not contained in Law Number 12 of 2011 concerning the Formation of Laws and Regulations as amended by Law Number 15 of 2019 concerning Amendments to Law Number 12 of 2011 concerning the Formation of Laws and Regulations. With this, the first thing that must answer is the question …


Problematika Pengisian Jabatan Menteri Yang Membidangi Birokrasi Di Indonesia, Sultan Sorik, Siska Windu Natalia, Erma Yustiyah, Anang Dwiatmoko Jun 2022

Problematika Pengisian Jabatan Menteri Yang Membidangi Birokrasi Di Indonesia, Sultan Sorik, Siska Windu Natalia, Erma Yustiyah, Anang Dwiatmoko

Jurnal Konstitusi & Demokrasi

This study examines the problems of filling ministerial positions in charge of the bureaucracy in Indonesia. The form of research used is normative juridical. From the research conducted, it was found that, 1) in the midst of the spirit of the State Civil Apparatus Law to implement the Merit System in human resource management, one of the important things that was forgotten was about filling in the ministerial positions in charge of bureaucracy, which is the spearhead of bureaucratic policy makers in Indonesia. Indonesia; 2) pressure from political parties was very influential in selecting ministers in the cabinet during the …


Daubert/Kumho Tire And The Legal Malpractice Expert Witness, Warren R. Trazenfeld, Robert M. Jarvis Jun 2022

Daubert/Kumho Tire And The Legal Malpractice Expert Witness, Warren R. Trazenfeld, Robert M. Jarvis

St. Mary's Journal on Legal Malpractice & Ethics

In legal malpractice cases, parties almost always end up using expert witnesses. Whether a particular legal malpractice expert is qualified to testify often is a hotly contested issue. In this Article, the authors provide recommendations for how to qualify a legal malpractice expert and how to challenge a legal malpractice expert’s qualifications.


“So, You Can Let Me Look At Your Phone?”: Detectives Obtaining Consent To Search Cell Phones, Stacy Dewald Jun 2022

“So, You Can Let Me Look At Your Phone?”: Detectives Obtaining Consent To Search Cell Phones, Stacy Dewald

Journal of Criminal Justice and Law

Issues regarding Fourth Amendment protections and police access to digital evidence have been an ongoing battle in the courts over the past few years. Despite growing literature on the importance of collecting digital evidence during criminal investigations, there is little research on how detectives obtain consent to get to digital evidence, particularly cell phones. The present study addresses this research gap by drawing upon participant-observation, in-situ interviews, and in-depth interviews of detectives in an American suburban police department. The data indicate that during suspect interviews, detectives attempt to obtain consent to search phones to avoid delays related to obtaining a …


Ethnographic Insight On The Construct Of Blackness: Heritage, ‘Home,’ Community, And Reality In Carver City-Lincoln Gardens, Tampa, Florida, 1928-2021, Lisa Katina Armstrong Jun 2022

Ethnographic Insight On The Construct Of Blackness: Heritage, ‘Home,’ Community, And Reality In Carver City-Lincoln Gardens, Tampa, Florida, 1928-2021, Lisa Katina Armstrong

USF Tampa Graduate Theses and Dissertations

This study is a community-engaged investigation of the experiences and impacts of Black people living in the segregated space called “Carver City-Lincoln Gardens.” This study aims to advance scholarly and mainstream conversations about one racialized community; it is a significant space because the Lincoln Gardens Area was explicitly designated by the Department of Defense as a residential space for Black veterans after World War II to purchase homes. By exploring this housing arrangement through an anthropological lens, the findings indicate that a mixed-method, collaborative ethnography reveals nuances that might be glossed over when communities are racialized. This study also seeks …


The Making Of Microcelebrities On Douyin: Multimodal Design And Online Identities In Informal English Instruction Videos, Yaqian Jiang Jun 2022

The Making Of Microcelebrities On Douyin: Multimodal Design And Online Identities In Informal English Instruction Videos, Yaqian Jiang

USF Tampa Graduate Theses and Dissertations

As the online education market continues to expand globally, teaching and advertising have converged in digital spaces in unprecedented ways. Driven by the attention economy, independent online educators must compete to cultivate a consumer base. To attract followers, they must engage not only in online teaching activities but also in self-branding practices. This dissertation explores how microcelebrities who teach English on Douyin utilize linguistic and other semiotic resources for both instructional and self-branding purposes.

To address this topic, I collected 84 informal English language instruction videos created by 14 different Douyin microcelebrities. Taking a social semiotic approach to multimodal discourse …


Florida Punks: Punk, Performance, And Community At Gainesville’S Fest, Michael Anthony Mcdowell Ii Jun 2022

Florida Punks: Punk, Performance, And Community At Gainesville’S Fest, Michael Anthony Mcdowell Ii

USF Tampa Graduate Theses and Dissertations

Fest is an annual punk rock music festival held in Gainesville, Florida. At Fest, participants engage in aesthetic creation and participation, negotiate identities, participate in political discourse, and forge communities. In this dissertation, I use theories of performance, framing, and culture to understand Fest. To do so, I theorize Fest using three approaches to performance: performance as an object of study, performance as a metaphor for everyday life and identity, and performance as a method of inquiry. Using these three approaches to performance, I understand Fest as performance, as a site that permits and constrains performances of self, identity, and …


An Assessment Of Transgender And Gender Non-Conforming Individuals Gender Affirming Health Care Practices In The Greater Tampa Bay, Sara J. Berumen Jun 2022

An Assessment Of Transgender And Gender Non-Conforming Individuals Gender Affirming Health Care Practices In The Greater Tampa Bay, Sara J. Berumen

USF Tampa Graduate Theses and Dissertations

The LGBTQ+ community faces discrimination and oppression throughout U.S. history and today. In particular, transgender and gender nonconforming (TGNC) individuals may face a variety of challenges when seeking biomedical health care tied to hostility, discomfort, lack of training, stigma, and denial of care at clinics or hospitals (Baker & Beagan, 2014; Lykens et al., 2018; Safer, 2021). TGNC individuals face medical gatekeeping when trying to access medical gender-affirming care (Aizura, 2018; Malatino, 2020). The research project aims to investigate these individuals' healthcare experiences, and access to both medicalized and nonmedicalized gender affirming health care practices.

In order to conduct this …


Hpv Infection And Vaccination: A Question-And-Answer Guide For School Nurses, Mendi England, Karlen E. Luthy, Michael S. Robinson Jun 2022

Hpv Infection And Vaccination: A Question-And-Answer Guide For School Nurses, Mendi England, Karlen E. Luthy, Michael S. Robinson

Student Works

This paper provides school nurses with a quick question-and-answer format guide to common HPV-related questions that are often asked by school aged children's parents.


217 E. 88th St. & 212-234 E. 89th St. Llc V. Keys Jun 2022

217 E. 88th St. & 212-234 E. 89th St. Llc V. Keys

All Decisions

No abstract provided.


Schwesinger V. Perlis Jun 2022

Schwesinger V. Perlis

All Decisions

No abstract provided.


Application Of Adult Learning Theory To Continuing Medical Education (Cme) Development: A Quantitative Study To Determine If Cme Developed With Adult Learning Theory Can Improve Test Utilization In The Pediatric Emergency Department, Brenda Davis Jun 2022

Application Of Adult Learning Theory To Continuing Medical Education (Cme) Development: A Quantitative Study To Determine If Cme Developed With Adult Learning Theory Can Improve Test Utilization In The Pediatric Emergency Department, Brenda Davis

Graduate Theses, Dissertations, and Capstones

Healthcare professions education is a multifaceted topic with a broad scope spanning from the undergraduate to professional level. The application of adult learning theory and interprofessional education are aspects of this topic where there is a deviation between what is happening at the undergraduate, graduate, and professional level. The intent of this quantitative experimental research study was to examine if the application of adult learning theory to the current continuing medical education/continuing education (CME/CE) model could improve the utilization of the respiratory pathogen panel (RPP) test in an urban pediatric acute care children’s hospital emergency department (ED) to a greater …


An Essential Role For Tungsten In The Ecology And Evolution Of A Previously Uncultivated Lineage Of Anaerobic, Thermophilic Archaea, Steffen Buessecker, Marike Palmer, Dengxun Lai, Joshua Dimapilis, Xavier Mayali, Damon Mosier, Jian-Yu Jiao, Daniel R. Colman, Lisa M. Keller, Emily St John, Michelle Miranda, Cristina Gonzalez, Lizett Gonzalez, Christian Sam, Christipher Villa, Madeline Zhuo, Nicholas Bodman, Fernando Robles, Eric S. Boyd, Alysia D. Cox, Brian St Clair, Zheng-Shuang Hua, Wen-Jun Li, Anna-Louise Reysenbach, Matthew B. Stott, Peter K. Weber, Jennifer Pett-Ridge, Anne E. Dekas, Brian P. Hedlund, Jeremy A. Dodsworth Jun 2022

An Essential Role For Tungsten In The Ecology And Evolution Of A Previously Uncultivated Lineage Of Anaerobic, Thermophilic Archaea, Steffen Buessecker, Marike Palmer, Dengxun Lai, Joshua Dimapilis, Xavier Mayali, Damon Mosier, Jian-Yu Jiao, Daniel R. Colman, Lisa M. Keller, Emily St John, Michelle Miranda, Cristina Gonzalez, Lizett Gonzalez, Christian Sam, Christipher Villa, Madeline Zhuo, Nicholas Bodman, Fernando Robles, Eric S. Boyd, Alysia D. Cox, Brian St Clair, Zheng-Shuang Hua, Wen-Jun Li, Anna-Louise Reysenbach, Matthew B. Stott, Peter K. Weber, Jennifer Pett-Ridge, Anne E. Dekas, Brian P. Hedlund, Jeremy A. Dodsworth

Life Sciences Faculty Research

Trace metals have been an important ingredient for life throughout Earth’s history. Here, we describe the genome-guided cultivation of a member of the elusive archaeal lineage Caldarchaeales (syn. Aigarchaeota), Wolframiiraptor gerlachensis, and its growth dependence on tungsten. A metagenome-assembled genome (MAG) of W. gerlachensis encodes putative tungsten membrane transport systems, as well as pathways for anaerobic oxidation of sugars probably mediated by tungsten-dependent ferredoxin oxidoreductases that are expressed during growth. Catalyzed reporter deposition-fluorescence in-situ hybridization (CARD-FISH) and nanoscale secondary ion mass spectrometry (nanoSIMS) show that W. gerlachensis preferentially assimilates xylose. Phylogenetic analyses of 78 high-quality Wolframiiraptoraceae MAGs from terrestrial and …


Pusatere V. The City Of Albany Jun 2022

Pusatere V. The City Of Albany

All Decisions

State supreme court in Albany declares local Good Cause eviction law null and void, holding that it is in conflict with parts of the RPL and RPAPL allowing termination after expiration of the tenancy.


Evaluation Of Medical Waste Management Before And During The Covid-19 Pandemic At Sentra Medika Hospital Cikarang, Enny Mar’Atus Sholihah, Amal Chalik Sjaaf Jun 2022

Evaluation Of Medical Waste Management Before And During The Covid-19 Pandemic At Sentra Medika Hospital Cikarang, Enny Mar’Atus Sholihah, Amal Chalik Sjaaf

Jurnal ARSI : Administrasi Rumah Sakit Indonesia

One of important things in managing Coronavirus Disease 2019 (COVID-19) pandemic is managing medical waste production. Medical waste management start from waste sort until waste manage by licensed parties, both of patients’ waste and health workers’ waste. Sentra Medika Hospital Cikarang is one of the referral hospitals that handles COVID-19. This research is a descriptive analytic study. Researchers analyze hazardous and toxic waste management before and during a pandemic by conducting interviews with the nursing unit and using secondary data. Secondary data was obtained from the hospital environmental health unit. There is no significant differences medical waste before and during …


Tackling Hotel Employees’ Turnover: A Moderated-Mediation Analysis Of Transformational Leadership, Organisational Embeddedness, And Community Embeddedness, Albert Amankwaa, Pi Shen Seet, Pattanee Susomrith Jun 2022

Tackling Hotel Employees’ Turnover: A Moderated-Mediation Analysis Of Transformational Leadership, Organisational Embeddedness, And Community Embeddedness, Albert Amankwaa, Pi Shen Seet, Pattanee Susomrith

Research outputs 2022 to 2026

Research in the last two decades suggests that job embeddedness may reduce employee turnover. However, little is known about whether this is the case in the hotel industry despite its high turnover rates. Besides, most studies have been conducted in the US, where the job embeddedness construct was developed and tested, suggesting the need to test the job embeddedness-turnover link in other contexts to improve its theoretical accuracy and generalisability. Additionally, while appropriate leader behaviours may enhance follower job embeddedness, empirical studies are sparse. Drawing from the Conservation of Resources theory, this study examines how transformational leaders may address hotel …


Perdebatan Teoritis Terhadap Perluasan Ruang Lingkup Keuangan Negara Di Indonesia, Sutan Sorik, Anang Dwiatmoko Jun 2022

Perdebatan Teoritis Terhadap Perluasan Ruang Lingkup Keuangan Negara Di Indonesia, Sutan Sorik, Anang Dwiatmoko

Jurnal Hukum & Pembangunan

The focus of this research is the expansion of the scope of state finance which currently still presents cross-thinking. The existence of a coherent understanding regarding the application of the financial legal status of a legal entity, whether it is related to state finances, regional finances, state-owned enterprises (BUMN) finances, regional-owned enterprises (BUMD) finances, or private finances is the background why this happens. In the development of the expansion of the scope of state finances, it became even stronger when the Constitutional Court also issued several decisions in response to requests for judicial review, starting from the Decision of the …


Pembagian Hasil Migas Melalui Cost Recovery Dan Gross Split Bagi Sebesar-Besarnya Kemakmuran Rakyat Menurut Uud Nri Tahun 1945, Athari Farhani, Faiqah Nur Azizah, Panggalih Usadhani, Faridh Kurniawan Jun 2022

Pembagian Hasil Migas Melalui Cost Recovery Dan Gross Split Bagi Sebesar-Besarnya Kemakmuran Rakyat Menurut Uud Nri Tahun 1945, Athari Farhani, Faiqah Nur Azizah, Panggalih Usadhani, Faridh Kurniawan

Jurnal Hukum & Pembangunan

Indonesia is a country with abundant natural resources. In oil and gas, two models of product sharing contract are known, namely through cost recovery and gross split. Through Permen of ESDM Number 12 of 2020 concerning the Third Amendment to Regulation of the Minister of Energy and Mineral Resources Number 8 of 2017 concerning Gross Split Production Sharing Contracts, which through this regulation the government makes it easy for contractor entrepreneurs to choose the Gross Split and Cost Recovery model schemes. Meanwhile, the 1945 Constitution of the Republic of Indonesia has outlined that natural resources are controlled by the state …


Pusaran Konflik Agraria Dan Model Resolusi Konflik Berbasis Keadilan Restoratif, Nurnaningsih Nurnaningsih Jun 2022

Pusaran Konflik Agraria Dan Model Resolusi Konflik Berbasis Keadilan Restoratif, Nurnaningsih Nurnaningsih

Jurnal Hukum & Pembangunan

Land acquisition for public purposes does not really provide a solution for the National Strategic Project (PSN). Many land conflicts continue to occur as a result of this. The construction of the Bener Dam in Wadas Village, Bener District, Purworejo Regency, Central Java Province is one of the conflicts over the PSN. For that we need a solution that guarantees justice and legal protection for the people in Indonesia.


Evaluasi Pelaksanaan Peradilan Daring Di Pengadilan Agama Jakarta Selatan Untuk Mengoptimalisasi Penyelesaian Perkara Perceraian Selama Pandemi Covid-19, Heru Susetyo, Satrio Alif Febriyanto, Rosalinda Estevani Kardinal Jun 2022

Evaluasi Pelaksanaan Peradilan Daring Di Pengadilan Agama Jakarta Selatan Untuk Mengoptimalisasi Penyelesaian Perkara Perceraian Selama Pandemi Covid-19, Heru Susetyo, Satrio Alif Febriyanto, Rosalinda Estevani Kardinal

Jurnal Hukum & Pembangunan

To facilitate judicial process implementation during the COVID-19 Pandemic, courts can be conducted online. Besides preventing mass gatherings that avoid during the COVID-19 Pandemic, online courts also have other benefits to shortening time and saving court costs. The study of online justice application at the South Jakarta Religious Courts aims to find out online justice application in the divorce cases settlement with the final result of an online justice application evaluation in the divorce cases settlement during the COVID-19 Pandemic that can be used as evaluation material for stakeholders so that online courts can conduct better in the future. The …


Cyber Security Challenges, Its Emerging Trends On Latest Information And Communication Technology And Cyber Crime In Mobile Money Transaction Services, Hakeem J. Pallangyo Jun 2022

Cyber Security Challenges, Its Emerging Trends On Latest Information And Communication Technology And Cyber Crime In Mobile Money Transaction Services, Hakeem J. Pallangyo

Tanzania Journal of Engineering and Technology (TJET)

This paper investigates the challenges emerging trends on latest Information and Communication Technology and cybercrime in mobile money transaction services in Tanzania. The objective of this is to evaluate the challenges associated with this rapid growth in ICT and to determine factors influencing Cybersecurity readiness and Cybercrimes in mobile money transaction services. Cyber Security plays a significant role in the field of Information and Communication Technology especially on mobile money transaction services. The study recognizes the provision of mobile money services by both telecommunication companies and local banks, the fact is that whenever we think about the cyber security, the …


Defunding Police Agencies, Rick Su, Anthony O'Rourke, Guyora Binder Jun 2022

Defunding Police Agencies, Rick Su, Anthony O'Rourke, Guyora Binder

Journal Articles

This Article contextualizes the police defunding movement and the backlash it has generated. The defunding movement emerged from the work of Black-led activists to reassert democratic control over policing and shift resources to social service agencies and other institutions serving community needs. In reaction, states have enacted anti-defunding bills checking local government reduction of law enforcement budgets. These anti-defunding measures continue a long tradition of state and federal control over local police spending, subverting local democratic control over police agencies. These limits include direct legal constraints on local police spending and indirect constraints through grants and authorization to collect fines, …


Nebraska Law Review 2022 Vol. 100, No. 4 -- Masthead & Table Of Contents Jun 2022

Nebraska Law Review 2022 Vol. 100, No. 4 -- Masthead & Table Of Contents

Nebraska Law Review

No abstract provided.


Algorithmic Decision-Making And Corporate Risk: Toward Transparency Through Corporate Disclosures, Kevin X. Kuhn Jun 2022

Algorithmic Decision-Making And Corporate Risk: Toward Transparency Through Corporate Disclosures, Kevin X. Kuhn

Nebraska Law Review

Consider this hypothetical scenario: a national car seller implements an algorithmic decision-making system to raise profits by ten percent in the next year. The algorithm will target the most competitive geographic market for a particular vehicle, recommend a price, and screen consumers for creditworthiness. The algorithm is immensely successful, and the corporation achieves its goal for increased profits. But a disturbing trend emerges when multiple consumers file lawsuits alleging discriminatory practices. Consumers claim that the corporation gave Black borrowers with otherwise equal credentials less favorable loan terms than white borrowers. Barred from bringing suit by a contractual arbitration provision and …


In Celebration Of Morrissey V. Brewer At Fifty: A Surprising University Of Nebraska College Of Law Back Story To The Prisoners’ Rights Due Process Landmark, Russell E. Lovell Ii Jun 2022

In Celebration Of Morrissey V. Brewer At Fifty: A Surprising University Of Nebraska College Of Law Back Story To The Prisoners’ Rights Due Process Landmark, Russell E. Lovell Ii

Nebraska Law Review

The year 2022 will be the fiftieth anniversary of the Supreme Court’s 1972 landmark prisoners’ rights case, Morrissey v. Brewer.1 Morrissey was a Fourteenth Amendment due process challenge involving an Iowan whose parole was revoked without a hearing.2 But long before Mr. Morrissey was in prison, a legislative internship at the University of Nebraska College of Law (Nebraska Law) in 1968 got me involved in researching and drafting the Nebraska Treatment and Corrections Act of 1969, including state parole law. The one area of parole law not reformed by the new legislation was parole revocation, a shortcoming that was the …


An Essential Role For Tungsten In The Ecology And Evolution Of A Previously Uncultivated Lineage Of Anaerobic, Thermophilic Archaea, Steffen Buessecker, Marike Palmer, Dengxun Lai, Joshua Dimapilis, Xavier Mayali, Damon Mosier, Jian-Yu Jiao, Daniel R. Colman, Anna-Louise Reysenbach, Multiple Additional Authors Jun 2022

An Essential Role For Tungsten In The Ecology And Evolution Of A Previously Uncultivated Lineage Of Anaerobic, Thermophilic Archaea, Steffen Buessecker, Marike Palmer, Dengxun Lai, Joshua Dimapilis, Xavier Mayali, Damon Mosier, Jian-Yu Jiao, Daniel R. Colman, Anna-Louise Reysenbach, Multiple Additional Authors

Biology Faculty Publications and Presentations

Trace metals have been an important ingredient for life throughout Earth’s history. Here, we describe the genome-guided cultivation of a member of the elusive archaeal lineage Caldarchaeales (syn. Aigarchaeota), Wolframiiraptor gerlachensis, and its growth dependence on tungsten. A metagenome-assembled genome (MAG) of W. gerlachensis encodes putative tungsten membrane transport systems, as well as pathways for anaerobic oxidation of sugars probably mediated by tungsten-dependent ferredoxin oxidoreductases that are expressed during growth. Catalyzed reporter deposition-fluorescence in-situ hybridization (CARD-FISH) and nanoscale secondary ion mass spectrometry (nanoSIMS) show that W. gerlachensis preferentially assimilates xylose. Phylogenetic analyses of 78 high-quality Wolframiiraptoraceae MAGs from …