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Sex-Specific Differences Resulting From High Fat Diet Observed In Microglial Responses Across Varying Brain Regions, Divya Komandooru, Divya Papaiya, Anshika Motiani, Shalet James, Zachary Lawrence

Undergraduate Student Symposium

Microglia are the resident immune cells in the nervous system, as they activate and release cytokines in response to immune challenges and maintain brain homeostasis. Consumption of a high fat diet and resulting metabolic disease (e.g. obesity and type 2 diabetes) alters microglial activity; however, studies have yet to explore sex differences in the effects of high fat diet/metabolic disease on microglia across several brain areas concurrently. This is vital given the heterogeneity of microglial distribution, morphology, and function across various pathological states. Male and female C57Bl/6J mice were fed either a low fat (LF; 10% fat) or high fat …

Sex-Specific Effects Of High-Fat And Ketogenic Diets On Cognition And Hippocampal Neurogenesis In C57b16/J Mice, Shalet James, Zachary Lawrence, Iliana Uribe, Ariana Hernandez, Eleanor Wind, Blanche Tamiya, Victoria Pulido-Correa, Natalia Noto, Yazmin Restropo

Undergraduate Student Symposium

Poor diet and metabolic diseases (obesity, Type 2 diabetes), are associated with increased risk of neurodegenerative and neuropsychiatric disorders, including Alzheimer’s disease, anxiety, and depression. Previous studies suggest that high-fat diet results in sex-specific effects in mice, including adult hippocampal neurogenesis, neuroinflammation, and performance on cognitive-behavioral tasks. The ketogenic diet, a popular weight loss regimen, is characterized by a low-carbohydrate, high-fat, and moderate-protein diet. Under carbohydrate restriction, the body enters ketosis, producing ketones bodies as a source of energy. While the ketogenic diet is very high in fat content, it may also possess neuroprotective properties against brain aging and neurodegenerative …

A Simple Method To Assess Porphyrin Basicity Using Competition Studies, Pranav Madadi, Ofek Gur, Ayush Desai

Undergraduate Student Symposium

Competition studies of three freebase porphyrins (H2OEP, H2OETPP, and H2TPP) were carried to assess their basicities. These porphyrins were selected because they are a good representation of natural occurring ones. In each experiment, the resultant UV-vis spectra were obtained by mixing and analyzing two porphyrins in a double-sided cuvette in which one was protonated using either trifluoroacetic acid (TFA) or hydrochloric acid (HCl) while the other was kept as a freebase. After mixing, the two porphyrins “compete” available protons, thus the most basic of them will acquire most of the protons. By adjusting the ratios of dications and freebases we …

Behavioral Responses Of Aplysia Californica To Three Amino Acids, Adrian Ditore

Undergraduate Student Symposium

For the sea slug Aplysia californica, chemical detection allows the location of food, avoidance of predators, and ability to encounter potential mates. This is an important sensory modality because other sensory structures (optical and acoustic) are limited. It is hypothesized that food is detected via concentration gradients of specific food-derived amino acids in the water column. This study examines the behavioral reactions of Aplysia to L-alanine, L-serine, and L-methionine at a concentration of 20 mM in contrast to a food stimulus. Test subjects were placed in an aquarium and 50 mL of the amino acid was added near the …

Are Students In Broward County, Fl, Who Get Less Sleep More Likely To Experience Depressive Symptoms?, Kelly Nyman, Alissa Lopez, Lauren Herrera

Undergraduate Student Symposium

It is commonly known that sleep can significantly affect overall health in both physical and mental states, but can getting less sleep be an attributing factor to high school students experiencing symptoms of depression? In this study, we will determine whether less sleep is associated with feelings of sadness or hopelessness which are common symptoms of depression. Finding a link between the two could allow us to implement sleep education programs that would lower rates of depression in high school students. Using data collected from the 2019 YRBSS from Broward County, Florida, data was run comparing high school students who …

Boldness And Endurace In Wild And Domesticated Sailfin Mollies, Claire Scobie

Undergraduate Student Symposium

Environment is known to influence physiological and behavioral differences, even within the same species. We compared the boldness and endurance levels of sailfin mollies (Poecilia latipinna) from domestic environments (pet stores) and wild habitats. We caught wild sailfin mollies from several freshwater sites in the South Florida area and purchased sailfin mollies from pet stores. We then used a boldness testing apparatus with a shelter and obstacles, recording each trial in a series of images. The time fish spent in latency (within the shelter while doors were open) was recorded and the recording of movement around the tank …

Effects On High Fat And Ketogenic Diet On Non-Alcoholic Fatty Liver Disease Pathology In Male And Female Mice, Nitish Dundigalla, Shalet James, Iliana Uribe, Zachary Lawrence, Ariana Hernandez, Blanche Tamiya, Eleanor Wind

Undergraduate Student Symposium

Nonalcoholic fatty liver disease (NAFLD) is a condition where excess fat builds up in the liver, affecting 25% of Americans. Interestingly, NAFLD is associated with neurological symptoms, including anxiety, depression, cognitive dysfunction/dementia. Given the high rates of these diseases, understanding risk and protective factors for NAFLD is crucial. Individuals with obesity, Type 2 Diabetes and related conditions are prone to NAFLD. While consumption of high fat/Western diet increases risk, there is evidence to suggest that ketogenic diet, which is high in fat but low in carbohydrates, may protect against NAFLD. Biological sex may influence NAFLD, but the evidence is conflicting …

Effect Of Methylphenidate On Alzheimer's And Dementia, Smrithi Mukund, Priyanka Prasanna, Aishwarya Muppoor, Maheen Khan

Undergraduate Student Symposium

Alzheimer's disease (AD), the most common form of dementia, is a neurogenerative disorder that causes impairments in cognitive abilities, behavior, and personality. AD is distinguished by extracellular plaques containing beta-amyloid proteins and intracellular neurofibrillary tangles containing hyperphosphorylated tau proteins. Current FDA-approved drugs for AD have questionable efficacy and carry potential risks. Methylphenidate (Ritalin), a stimulant drug currently used to treat ADHD and narcolepsy, has been used off-label to treat AD patients, primarily for relieving the symptom of apathy. While our literature review suggests that relatively short-term (< 6 weeks) use in patients with AD and related diseases is safe and effective in clinical trials, little is known about the longer-term effects of the drug in this population. More work is needed, as there is also a paucity of animal research investigating chronic effects of methylphenidate in rodent models of AD and related disorders. Findings from animal studies in wild-type rodents on chronic use of methylphenidate, as well as cell culture studies, have reported adverse effects of methylphenidate, including increases in neuroinflammation and oxidative stress, neurodegeneration, vascular impairments (e.g. blood brain barrier dysfunction), and altered cognitive-behavioral performance. While the clinical significance of methylphenidate treatment in AD patients may be unclear, studies over longer periods of time including testing on animal models will help us understand how methylphenidate treatment can affect our brain activity in the long term and how it can be administered more safely and effectively.

Evaluation Of Mdm2 Regulation On Aurkb Expression And Cell Death In Lung Cancer, Lohita Sunkara, Saaketh Palli

Undergraduate Student Symposium

This research examines the molecular mechanisms by which MDM2 regulates AURKB in lung cancer cells. In the United States alone nearly 238,340 people will be diagnosed with lung cancer in 2023. Hence, the need for new anti-cancer drugs and treatment methods is still persisting to fight against this deadly disease. This study explores the intracellular link between MDM2 and AURKB, to determine how manipulating this molecular mechanism can lead to cell cycle arrest and cancer cell death.

Lung cancer cells were treated with RG7388, an MDM2 inhibitor, and Barasertib, an AURKB inhibitor. Following treatments, the cells were lysed, and western …

Exercise As A Natural Kappa Opioid Receptor Antagonist To Treat Binge Drinking Withdrawal, Eleanor Wind

Undergraduate Student Symposium

Binge drinking is a serious public health problem in the United States that is especially prevalent among college students. The results of a national survey reported 33% of full-time college students aged 18-22 engaged in binge drinking during the previous month. Positive results have been demonstrated in clinical trials that treated alcohol abuse with aerobic exercise. Research in animal models aims to answer remaining questions regarding implementation across sexes and age groups by studying underlying neurobiological mechanisms. One such mechanism is the kappa opioid receptor (KOR) of the endogenous brain opioid system. In animal models, administration of a KOR antagonist …

Investigating The Metal-Binding Properties Of Chitosan And Bisphosphonate Functionalized Chitosan, Andrew Brandea, Ashley Rodriguez

Undergraduate Student Symposium

Functionalized polymers can be used as chelating agents to remove heavy metal contaminants from water. Chitosan is a biologically derived and commercially available polymer with a wide variety of clinical and industrial applications. The primary amine pendant group on the repeat unit of chitosan has the potential to act as a metal binding site due to the presence of a lone pair on the nitrogen atom. The purpose of this study was to functionalize deacetylated chitosan with a tridentate group to enhance its metal binding potential through the addition of more ligand binding sites. Specifically, a multicomponent Moedritzer-Irani reaction was …

Munkgeeroachy: A Gordonia Rubripertincta Bacteriophage Isolated From Soil, Ashley Janke

Undergraduate Student Symposium

There are many bacteria that are growing resistant to antibiotics. Therefore, there is an urgent need for alternative therapies. Bacteriophage therapy, the use of viruses to treat bacterial infections, is a promising alternative. The goal of this project was to isolate and identify phages from soil samples that infect Gordonia rubripertincta. G. rubripertincta is a gram-positive bacterium that is typically found in soil and aquatic habitats. Gordonia species are rapidly becoming associated with human and animal diseases. Infections of G. rubripertincta have typically been found after various surgical procedures such as the applications of catheters, or the use of …

Red Dog Loess, A Last Glacial Dust Deposit, White River Badlands, S.D., Lakshmi Srikari Ravulaparthi, Ian Chang, Haley Perkins, John Nichols, Curtis Frye

Undergraduate Student Symposium

Understanding the detrimental effects of prehistoric climate change is essential to understanding our potential for future global climate disasters. During the Last Glacial Period (LGP) beginning around 70 thousand years ago, as Earth’s temperature dropped, the midcontinent grew increasingly dry, resulting in the ecological collapse of the Great Plains grassland ecosystems. Due to the breakdown of the grasslands, wind eroded the Great Plains, depositing vast amounts of windblown dust (loess). These loess deposits form valuable midcontinental agricultural deposits in Nebraska and Iowa. This study aims to identify the origin of the loess found on mesas in the White River Badlands …

Sargassum Accumulation At Von D. Mizell-Eula Johnson State Park, Giavanna Iglio, Jarod Johnson, Kayla Jones, Kaleb Myers, Juliana Ottens-Rivera, Aidan Pflieger, Manuel Ploner, Kevin Rafferty, Maura Wondra

Undergraduate Student Symposium

Sargassum is a typically floating phaeophyte (brown alga) with important ecosystem functions. It is a producer, generating biomass via photosynthesis, and providing habitat for small epipelagic organisms in the open ocean. In August 2022, large amounts of Sargassum were seen on the beach at the northern end of Von D. Mizell-Eula Johnson State Park (VDMEJSP), Dania Beach, Florida, up to 152cm thick. This disturbs the intertidal environment, which is typically composed of carbonate sands, and smothers infauna. Furthermore, this creates ephemeral habitats for species not typically associated here. The extent of Sargassum deposits was measured from 7 September through 25 …

Sleep Health Matters For All: Wellness Intervention For The Pediatric Down Syndrome Population, Mahima Dave

Undergraduate Student Symposium

Down syndrome (DS) is the most prevalent chromosomal disorder in which an extra copy of chromosome-21 disrupts the development of intellectual abilities. Unique physical manifestations places children with DS at high risk for obstructive sleep apnea (OSA) in which repeated blockages of the upper airway cause difficulty breathing during sleep. 80% of children with DS are afflicted with OSA, and this increases incidences of nocturnal sudden cardiac arrest by 2.57-fold compared to the general population. Manifestations of OSA, such as cognitive impairment and cardiovascular disease, are common in the DS population. However, this often obscures the diagnosis. During May 2021, …

An Optimal Strategy For Using Mixture-Based Combinatorial Library Data To Find T-Cell Epitopes, Sofia Celis

Undergraduate Student Symposium

Positional scanning libraries are well established in their use for peptide sequence identification and binding site optimization. Their use in identifying T cell stimulatory peptides has also previously been explored. Here we present a retrospective analysis on the results of mixture based positional scanning libraries of T-cell stimulatory peptides done before 2019 in order to identify an optimal strategy for using mixture based combinatorial library data to find T cell epitopes. The screening of positional scanning libraries against CD4+ and CD8+ T cell clones was analyzed using biometrical analysis approaches of various kinds in order to evaluate their relative effectiveness …

Comparison Of Behavior Of Mangrove Mosquitofish Across Their Range And Identification Of Their Hybridization With Eastern Mosquitofish Using Genital Morphology, Rose Leeger

Undergraduate Student Symposium

The Mangrove Mosquitofish is found in many brackish and freshwater ecosystems surrounding southeast Florida and Cuba. Historical range distribution in Florida has found these fish solely in the Florida Keys and parts of Miami. This research provides an update to the northernmost range that Mangrove Mosquitofish have been observed. As the name implies, Mangrove Mosquitofish reside in areas of critical habitat: Mangrove Forests. These forests are constantly battling habitat loss and reduction due to increased urbanization in native areas. Mangrove Mosquitofish are poecillid fish species with a modified anal fin called a “gonopodium” that allows for internal fertilization. This research …

Pass The Salt: Problem-Resolution Lawyering Across The Twenty-First Century Law Curriculum, Kris Franklin, F. Peter Phillips

Pepperdine Dispute Resolution Law Journal

Attorneys work with clients to resolve problems. Legal education can help prepare law graduates to do that work. As an added bonus, doing so would in turn help law students understand and retain the subjects they study. Law professors who teach alternative dispute resolution, lawyering skills, clinics, and sometimes traditional doctrinal courses, have all called for greater inclusion of dispute resolution in the law school curriculum. Some have urged the introduction of specific courses to prepare contemporary law students to work as problem resolvers. This Article builds on these and other calls for reform, but urges a genuine reconceptualization of …

Spartan Daily, April 5, 2023, San Jose State University, School Of Journalism And Mass Communications

Spartan Daily, 2023

Volume 160, Issue 27

Synthesis And Evaluation Of Biologically Active Phosphorous Heterocycles And Lipid A Antagonist Compounds, Saroj Kafle

Dissertations

Cyclophostin and cyclipostins are naturally occurring organophosphates with common structural core units and different phosphate ester substituents. Cyclophostin is a cyclic phosphate triester with a chiral center at phosphorous and C-3a carbon atom while the cyclipostins have characteristic alkyl substituents with varying chain lengths at the phosphate center. Long-chain substituted monocyclic phosphate (2b) and phosphonate (2a) analogs of cyclophostin and cyclipostin are promising inhibitors against serine hydrolases acetylcholinesterase with an IC₅₀ in the nanomolar range.

Biologically active β-lactones and lactams owe their activity, in part, to ring strain. The ring opening of β-lactones and lactams …

Suffolk Journal, Vol.86, No. 16, 4/5/2023, Suffolk Journal

Suffolk Journal

No abstract provided.

United Refining Co V. Epa

2023 Decisions

Agency

Tara Scott V. Vantage Corp

2023 Decisions

USDC for the District of Delaware

David Wilson V. Joseph Biden,Jr.

2023 Decisions

USDC for the District of New Jersey

Usa V. Darnell Doss

2023 Decisions

USDC for the Middle District of Pennsylvania

Carl Hemphill V. Landmark American Insurance Co

2023 Decisions

USDC for the Eastern District of Pennsylvania

Joe Thorpe V. Township Of Salisbury

2023 Decisions

USDC for the Eastern District of Pennsylvania

Implementation Of Established Guidelines To Determine The Correlation Of Antipsychotic Use And Fall Rate Occurrence Amongst Inpatient Long-Term Care Residents, Cynthia L. Hardy

Dissertations

The use of psychotropic medications may impact fall rates among senior adults. Despite evidence-based approaches to minimize psychotropic medications use, the project site had a gap in implementing one leading to high fall rates. The purpose of this quantitative quasi-experimental quality improvement project was to determine if the implementation of the Center for Assisted Living Guidelines for Reducing the Use of Antipsychotic Medications in Nursing Homes would impact fall rates when compared to current practice among geriatric patients in a long-term care facility in rural New Mexico over four weeks. Neuman's Systems Model provided the theoretical underpinnings for this project.

What The Heller Is Going On With The Second Amendment: Are Licensing Requirements Living Up To The Heller Standard?, Josue Barron

Texas A&M Journal of Property Law

The full extent and guarantees of the Second Amendment have yet to be understood in light of modern advances in weaponry. Further, there is little Supreme Court precedent to aid in defining the scope of the Second Amendment. With challenges to restrictions on concealed carrying of firearms in public, the Second Amendment requires much clarification. Federal circuit courts are divided on how to apply the Second Amendment to firearm licensing schemes and differ on the interpretation of the Heller decision. This Note provides guidance on understanding the core protection of the Second Amendment and the presumptions left by the Supreme …

The Marathon Continues: Texas Nil Has Room To Grow, Johnathon Blaine

Texas A&M Journal of Property Law

College athletes are now permitted to profit off their name, image, and likeness. However, while a hodgepodge of different regulations exists state-by-state and Congress continues to drag its feet to pass a federal framework, Texas restricts college athletes from maximizing their name, image, and likeness earning potential. This Comment proposes improvements to Senate Bill 1385 that would allow college athletes in Texas to partner with the same categories of “taboo” products as their respective university and to endorse products from competing brands, provided such endorsement is outside of a university-sponsored event, with an exception allowing unrestricted endorsement of footwear. This …