Digital Commons Network^™

On The Origin And Evolution Of The Mosquito Male-Determining Factor Nix, James K. Biedler, Azedah Aryan, Yumin Qi, Aihua Wang, Ellen O. Martinson, Daniel A. Hartman, Fan Yang, Atashi Sharma, Katherine S. Morton, Mark Potters, Chujia Chen, Stephen L. Dobson, Gregory D. Ebel, Rebekah C. Kadling, Sally Paulson, Rui-De Xue, Michael R. Strand, Zhijian Tu

Entomology Faculty Publications

The mosquito family Culicidae is divided into 2 subfamilies named the Culicinae and Anophelinae. Nix, the dominant male-determining factor, has only been found in the culicines Aedes aegypti and Aedes albopictus, 2 important arboviral vectors that belong to the subgenus Stegomyia. Here we performed sex-specific whole-genome sequencing and RNAseq of divergent mosquito species and explored additional male-inclusive datasets to investigate the distribution of Nix. Except for the Culex genus, Nix homologs were found in all species surveyed from the Culicinae subfamily, including 12 additional species from 3 highly divergent tribes comprising 4 genera, suggesting Nix originated at least 133 to …

Genome-Wide Significant Risk Loci For Mood Disorders In The Old Order Amish Founder Population, Elizabeth M. Humphries, Kwangmi Ahn, Rachel L. Kember, Fabiana L. Lopes, Evelina Mocci, Juan M. Peralta, John Blangero, David C. Glahn, Fernando S. Goes, Peter P. Zandi

School of Medicine Publications

Genome-wide association studies (GWAS) of mood disorders in large case-control cohorts have identified numerous risk loci, yet pathophysiological mechanisms remain elusive, primarily due to the very small effects of common variants. We sought to discover risk variants with larger effects by conducting a genome-wide association study of mood disorders in a founder population, the Old Order Amish (OOA, n = 1,672). Our analysis revealed four genome-wide significant risk loci, all of which were associated with >2-fold relative risk. Quantitative behavioral and neurocognitive assessments (n = 314) revealed effects of risk variants on sub-clinical depressive symptoms and information processing speed. …

Muc13 Drives Cancer Aggressiveness And Metastasis Through The Yap1-Dependent Pathway, Kyle Doxtater, Manish Tripathi, Radhiki Sekhri, Bilal B. Hafeez, Sheema Khan, Nadeem Zafar, Stephen Behrman, Murali M. Yallapu, Meena Jaggi, Subhash C. Chauhan

School of Medicine Publications

Anchorage-independent survival after intravasation of cancer cells from the primary tumor site represents a critical step in metastasis. Here, we reveal new insights into how MUC13-mediated anoikis resistance, coupled with survival of colorectal tumor cells, leads to distant metastasis. We found that MUC13 targets a potent transcriptional coactivator, YAP1, and drives its nuclear translocation via forming a novel survival complex, which in turn augments the levels of pro-survival and metastasis-associated genes. High expression of MUC13 is correlated well with extensive macrometastasis of colon cancer cells with elevated nuclear YAP1 in physiologically relevant whole animal model systems. Interestingly, a positive correlation …

A Sound Mind In A Sound Body: A Novel Concept Unravelling Heterogeneity Of Depression, Gabor Hullam, Zsofia Gal, Xenia Gonda, Tamas Nagy, Andras Gezsi, Isaac Cano, Sandra Van Der Auwera, Mikko Kuokkanen, Peter Antal, Gabriella Juhasz

School of Medicine Publications

Depression is a highly prevalent and debilitating condition, yet we still lack both in-depth knowledge concerning its etiopathology and sufficiently efficacious treatment options. With approximately one third of patients resistant to currently available antidepressants there is a pressing need for a better understanding of depression, identifying subgroups within the highly heterogeneous illness category and to understand the divergent underlying biology of such subtypes, to help develop and personalise treatments. The TRAJECTOME project aims to address such challenges by (1) identifying depression-related multimorbidity subgroups and shared molecular pathways based on temporal disease profiles from healthcare systems and biobank data using machine …

Whole-Genome Sequencing Uncovers Two Loci For Coronary Artery Calcification And Identifies Arse As A Regulator Of Vascular Calcification, Paul S. De Vries, Matthew P. Conomos, Kuldeep Singh, Christopher J. Nicholson, Deepti Jain, Natalie R. Hasbani, Wanlin Jiang, Sujin Lee, Christian L. Lino Cardenas, Sharon M. Lutz, John Blangero

School of Medicine Publications

Coronary artery calcification (CAC) is a measure of atherosclerosis and a well-established predictor of coronary artery disease (CAD) events. Here we describe a genome-wide association study of CAC in 22,400 participants from multiple ancestral groups. We confirmed associations with four known loci and identified two additional loci associated with CAC (ARSE and MMP16), with evidence of significant associations in replication analyses for both novel loci. Functional assays of ARSE and MMP16 in human vascular smooth muscle cells (VSMCs) demonstrate that ARSE is a promoter of VSMC calcification and VSMC phenotype switching from a contractile to a calcifying or osteogenic phenotype. …

Metabolic Phenotyping Of Bmi To Characterize Cardiometabolic Risk: Evidence From Large Population-Based Cohorts, Habtamu B. Beyene, Corey Giles, Kevin Huynh, Tingting Wang, Michelle Cinel, Natalie A. Mellett, Gavriel Olshansky, Thomas G. Meikle, Gerald F. Watts, John Blangero

School of Medicine Publications

Obesity is a risk factor for type 2 diabetes and cardiovascular disease. However, a substantial proportion of patients with these conditions have a seemingly normal body mass index (BMI). Conversely, not all obese individuals present with metabolic disorders giving rise to the concept of “metabolically healthy obese”. We use lipidomic-based models for BMI to calculate a metabolic BMI score (mBMI) as a measure of metabolic dysregulation associated with obesity. Using the difference between mBMI and BMI (mBMIΔ), we identify individuals with a similar BMI but differing in their metabolic health and disease risk profiles. Exercise and diet associate with mBMIΔ …

The Use Of Stem Gaming To Promote Hearing Health In Youth, Catelyn D. Tweeten

Doctoral Scholarly Projects & Capstones

Hearing loss prevention programs targeting youth have been implemented in many forms to teach younger populations about hearing health to prevent noise-induced hearing loss. Some hearing loss prevention programs, such as Dangerous Decibels®, are based on health communication science and aim to not only increase youth’s knowledge about noise-induced hearing loss, but positively change their attitudes, beliefs, and behaviors towards safe listening practices. The purpose of this project was to investigate how an educational computer game called Song of the Starbird could potentially be used as an effective hearing health promotion tool for students with normal hearing and students with …

Examining The Microbial Diversity Of A Generalist Ant Along An Urban-Rural Gradient, Abdol-Munim Abazid

Honors Theses

After sampling the exoskeleton microbiomes of 225 ants from 3 different locations along a rural-urban gradient in the Chattanooga area, a photo library was collected and microbial diversity and morphospecies evenness of each location was determined. It was determined that there was no significant difference in either microbial diversity or morphospecies evenness between sites along the rural-urban gradient, even after adjusting data to be between only rural and urban areas. Urbanization was quantified using % impervious surface; however, a future study could quantify urbanization by other means, such as soil nutrient composition. Additionally, it was determined that there was no …

From Blue Skies To Gray Areas: Examining Privacy And Property Law In The Age Of Drone Technology, Cheng-Chi (Kirin) Chang

Richmond Journal of Law & Technology

This article presents a comprehensive analysis of the legal frameworks governing the use of drones in both the United States and the European Union, and the privacy and property rights issues that arise from their use. The article emphasizes the importance of balancing the benefits of drone technology with the protection of individual rights. The regulatory frameworks governing drones are compared between the two jurisdictions, highlighting key similarities and differences. Furthermore, the article delves into the legal challenges surrounding drone usage and property rights, offering several solutions to address privacy concerns. In conclusion, the article highlights the need for a …

Using A Toxic Aging Coin To Assess Hexavalent Chromium-Induced Neurotoxicity., Samuel Thomas Vielee

Electronic Theses and Dissertations

We are facing an aging crisis, with 20% of the U.S. population projected to be geriatric (65+) by 2030 and live another 40+ years. Age-related diseases accompany a growing geriatric population, emphasizing the need to understand their etiology. Environmental pollutants compound this crisis by: 1) geriatrics are more susceptible, exacerbating age-related diseases and comorbidities, and 2) they accelerate biological aging, inducing age-related diseases at younger ages. We address this crisis using a ‘toxic aging coin’ approach; heads examines how age impacts toxicity, tails examines how chemicals accelerate aging. This thesis applies the heads side for Cr(VI)-induced neurotoxicity across ages. We …

Breaking Virulent: The Coincidental Evolution Of Virulence Factors In Bacteria., Rhiannon Emmanuelle Cecil

Electronic Theses and Dissertations

Understanding how innocuous organisms can evolve to be pathogenic to humans is of increasing global concern. Further, understanding how existing pathogens may evolved to be more virulent is also vital to our ability to provide healthcare to people afflicted with diseases that promote chronic bacterial infections, such as cystic fibrosis. With the rise of antibiotic resistance in both bacteria and fungi it is paramount that new therapeutics are identified. Understanding what mutations occur that result in increased virulence in microbes can potentially provide new targets for antimicrobial drugs to combat antibiotic resistance. The Coincidental Evolution Hypothesis is a fundamental hypothesis …

A Study Of Group Effectiveness In A Virtual Reality Environment., Dalton Clay Sparks

Electronic Theses and Dissertations

Virtual Reality (VR) technology has revolutionized the study of team effectiveness, yet many studies in this domain lack a comprehensive approach, focusing narrowly on performance metrics. This thesis investigates VR team effectiveness by integrating traditional Team Effectiveness frameworks with VR-specific dynamics. Utilizing Data Envelopment Analysis (DEA) and Group Style Inventory (GSI), this study assesses teams within a VR environment, specifically a multiplayer submarine simulation game, IronWolfVR. Fifteen groups, totaling 45 participants, were observed for their collaborative gameplay, incorporating physiological measures (Heart Rate and Electrodermal Activity), team coordination dynamics, and various survey-based assessments (demographics, System Usability Scale, Simulator Sickness Questionnaire, and …

From "Smart Talk" To "Living Well": Commonplaces And Their Role In Narratives Of Rare Disease., Caitlin E. Ray

Electronic Theses and Dissertations

As healthcare becomes more complex, automated, and bureaucratic, patients often suffer from a lack of resources, agency, and visibility when seeking medical care. Rhetoric and Composition, specifically the subfield of Rhetoric of Health and Medicine (RHM), is interested in studying and intervening into such issues. One way to challenge our current understanding of healthcare is to consider how the rare disease patient experience reveals the gaps, limitations, and assumptions of illness and health. I argue here that through rare diseases, rhetoricians of health and medicine can better understand the representation, advocacy, and patient experience within healthcare, and potentially lead to …

Computational Investigations Of Bond Breaking Processes Using Dft And Td-Dft Approaches., Saurav Parmar

Electronic Theses and Dissertations

The efficient application of DFT and TD-DFT has been harnessed to study bond-breaking processes in some molecules which play a prominent role in enzymatic reactions. The first application includes Radical S-adenosyl methionine (SAM) enzymes which are fundamentally important sources of organic radicals to initiate diverse radical reactions. Recently a bio-organometallic intermediate (Ω) that contains an Fe‒C bond has been characterized and shown to be a common feature of radical SAM enzymes. The strength of Fe‒C bond in Ω has been computed using broken-symmetry density functional theory (BS‒DFT). Additionally, Fe‒C bond dissociation energy (BDE) in Ω has been compared to that …

Submandibular Salivary Gland Vulnerability: Unveiling Aflatoxin B1-Induced Toxicity And Rosmarinus Officinalis 'S Protective Role, Mohamed Shamel, Mahmoud Al Ankily, Mohamed Mansy, Robert Fua Angeles

Dentistry

No abstract provided.

An Economic And Regulatory Analysis Of Gene Therapy Products Authorized By The Us Food And Drug Administration And The European Medicines Agency, Buthainah Ghanem

Pharmaceutical Sciences (PhD) Dissertations

Background: Gene therapy has recently emerged as an alternative for preventing and treating disease. Concerns have been expressed about the clinical value and the high cost of gene therapies.

Objective: This study assessed the authorization process of gene therapy products by the Food and Drug Administration (FDA) and the European Medicines Agency (EMA), evaluated the post-marketing safety signals reported to the FDA Adverse Event Reporting System (FAERS), and assessed the cost-effectiveness analysis of three gene therapies axicabtagene ciloleucel(axi-cel), lisocabtagene maraleucel (liso-cel), and tisagenlecleucel (tisa-cel).

Research Design: For aim 1; data was collected using regulatory information from the FDA and the …

U-Glide Program Fall 2023, Unf Office Of Undergraduate Research, Unf Graduate School

Undergraduate – Graduate Learning, Inquiry, and Distinctive Experimentation (U-GLIDE) Symposium Programs

No abstract provided.

A Cancer-Specific Study On The Differentially Expressed Protein-Protein Interactions Of Fumarate Hydratase, Sydney Lac

Dissertations and Theses (Open Access)

Fumarate hydratase (FH) is an enzyme used in the Krebs Cycle to convert fumarate to malate, and it is controlled by the FH gene. In this paper, we will investigate its role in Uterine Corpus Endometrial Carcinoma (UCEC) and how FH-deficient cells affect tumorigenesis. It is well-established that FH has been extensively studied in connection with renal cell carcinoma, skin and uterine leiomyomas, pheochromocytoma, and paraganglioma. However, we aim to construct an interaction network of significant genes related to the FH gene under conditions of FH deficiency in the Kreb Cycle. Creating an interactive network that illustrates the interconnectedness of …

Characterizing The Regulation Of Candida Albicans Hyphal Morphogenesis In The Context Of Host Macrophages, Hannah Wilson

Dissertations and Theses (Open Access)

Candida albicans is a commensal fungus that resides on the skin, mucosal surfaces, and in the gut of an estimated 80% of individuals. Though generally harmless, C. albicans is capable of causing uncomfortable mucosal infections as well as deadly disseminated infections depending on the immune status of the host. Although antifungal therapeutics exist, the mortality rate associated with disseminated disease still lingers around 50%. Further, C. albicans is the fourth most common cause of all bloodstream infections and continues to present as a major clinical issue with less than satisfactory treatment options. For this reason, it is imperative to understand …

Amyloid Fibrils Of Human Fgf-1 Induced By Different Detergents, Zeina Ismael Ibrahem Alraawi

Graduate Theses and Dissertations

Nature achieves molecular self-assembly through the ordered growth of nanoscale building blocks with high efficiency to fabricate macromolecular architectures. One example of self- assembly is peptides folding onto protein is one of the most astounding biological self-assembly processes. When proteins aggregate to form amyloid fibers, the secondary structure of the protein converts from its native state to a cross-beta-sheet. Fibroblast growth factors (FGFs) possess an essential role in neuronal survival during development. In addition, they are involved in neural stem cell (NSC) proliferation. Fibroblast growth factors (FGFs) are well known to be synthesized in the central nervous system (CNS) and …

Invasion Control Tangled In Knots: How An Oberlin Student And The Outside World Tackled The Knotweed Problem, Michael E. Harvey

The Synapse: Intercollegiate science magazine

No abstract provided.

Issue 38

The Synapse: Intercollegiate science magazine

No abstract provided.

Front Matter

The Synapse: Intercollegiate science magazine

No abstract provided.

Annual Report 2022-2023, Tennessee. Bureau Of Investigation

Annual Report

No abstract provided.

Computational Molecular Docking Studies Of Small Molecule Inhibitors With The Sars-Cov-2 Spike Protein Variants: In-Silico Drug Discovery Using Virtual Screening And Drug Repurposing Approaches, Grace Gupta

Computational and Data Sciences (MS) Theses

The pandemic caused by the emergence of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in 2019 has caused a global public health crisis of nearly unprecedented scale. In the years following the outbreak, the scientific community has mobilized to develop several vaccines and treatments. Drug repurposing as a strategy for drug development has produced many of the current therapeutic options. The greatest challenge to designing a therapeutic inhibitor of SARS-CoV-2 is the shifting mutational landscape of the virus as it evolves. In this study, we focus on the spike protein as a target for potential inhibitors. We explore two …

Characterization Of Human Fibroblast Growth Factor 2 Variant To Determine Effects On Structure, Stability, And Cell Proliferation, Ryan Layes

Graduate Theses and Dissertations

Fibroblast growth factors (FGFs) are a family of cell signaling proteins conserved across multiple species. Each individual FGF elicits different cellular functions including, but not limited to, proliferation, migration, differentiation, angiogenesis, and wound healing. One of the most studied members, fibroblast growth factor 2 (FGF2), has demonstrated substantial wound healing capacity in a wide range of tissues including skeletal, muscular, neural, respiratory, epithelial, and cardiovascular. This ability makes FGF2 a potential therapeutic for a wide range of conditions and injuries. However, due to a short half-life at room temperature, therapeutic use of FGF2 is limited. It has been demonstrated that …

Wild Sea Cucumber Trade In Rural Madagascar: Consequences For Conservation And Human Welfare, Emily Rothamel, Cortni Borgerson, Delox Rajaona, Be Noel Razafindrapaoly, Be Jean Rodolph Rasolofoniaina, Colette Feehan

Department of Anthropology Faculty Scholarship and Creative Works

Overexploitation of fisheries is a challenge to both marine conservation and human welfare. The trade of sea cucumbers is a multi-billion-dollar market, spanning over 70 countries. Global concern over unsustainable sea cucumber trade led to the inclusion of three species in CITES Appendix II, two of which are found in Madagascar (Holothuria fuscogilva and Holothuria nobilis). We used 792 structured interviews (2015–2020) to study the intensity of wild sea cucumber collection and exportation in rural northeastern Madagascar, where 30% of all (terrestrial and aquatic) native species are threatened with extinction. Wild sea cucumber collection was common and increased tenfold during …

The Role Of Governance In Rewilding The United States To Stem The Biodiversity Crisis, Shelby C. Carlson, John A. Vucetich, L Mark Elbroch, Shelby Perry, Lydia A. Roe, Tom Butler, Jeremy T. Bruskotter

Michigan Tech Publications

A critical but underattended feature of the biodiversity crisis is the contraction of geographic range experienced by most studied terrestrial vertebrates. In the United States, the primary policy tool for mitigating the biodiversity crisis is a federal law, the Endangered Species Act (ESA). For the past two decades, the federal agencies that administer the ESA have interpreted the act in a manner that precludes treating this geographic element of the crisis. Therefore, the burden of mitigating the biodiversity crisis largely falls on wildlife agencies within state government, which are obligated to operate on behalf of the interests of their constituents. …

Analysis Of Non-Kinship Foster Parent Training Needs And Their Support Of Foster Youth: An Occupational Therapy Perspective, Mackenzie King, Pam Kasyan-Howe, Kristin Domville, Amy Paul-Ward

Fall 2023 Virtual OTD Capstone Symposium

This poster discusses the training needs of non-kinship foster parents and how they support the youth in their care. A quantitative survey was used to find relationships between experience fostering and foster parent preparedness or desire for additional training. The survey also included a section for participants to write down their training needs; themes from this question are found on the poster. The poster further discusses future recommendations and implications for OT practice in working with this population.

Phylogenomics Reveals The History Of Host Use In Mosquitoes, John Soghigan, Charles Sither, Silvia Andrade Justi, Gen Morinaga, Brian K. Cassel, Christopher J. Vitek, Todd P. Livdahl, Siyang Xia, Andrea Gloria-Soria, Jeffrey R. Powell, Thomas Zavortink, Christopher M. Hardy, Nathan D. Burkett-Cadena, Lawrence E. Reeves, Richard C. Wilkerson, Robert R. Dunn, David K. Yeates, Maria Anice Sallum, Brian D. Byrd, Michelle D. Trautwein, Yvonne-Marie Linton, Michael H. Reiskind, Brian M. Wiegmann

Biology

Mosquitoes have profoundly affected human history and continue to threaten human health through the transmission of a diverse array of pathogens. The phylogeny of mosquitoes has remained poorly characterized due to difficulty in taxonomic sampling and limited availability of genomic data beyond the most important vector species. Here, we used phylogenomic analysis of 709 single copy ortholog groups from 256 mosquito species to produce a strongly supported phylogeny that resolves the position of the major disease vector species and the major mosquito lineages. Our analyses support an origin of mosquitoes in the early Triassic (217 MYA [highest posterior density region: …