Digital Commons Network^™

Development Of The Embryonic Left-Right Organizer: Identifying Regulators Of Proliferation During Development Of The Embryonic Left-Right Organizer, Amanda Marzouk, Edward Guy Iv, Jeffery D. Amack

The Crown: Syracuse Honors Research Journal

No abstract provided.

Carbon Sequestration, Conservation, And Capitalism: An Analysis Of West Virginia’S Senate Bill 162, Heleina Cicero

The Crown: Syracuse Honors Research Journal

No abstract provided.

The Crown, Complete Issue - V1, The Crown

The Crown: Syracuse Honors Research Journal

No abstract provided.

The Effect Of Db1976 On The Fibrotic Response In Three-Spined Stickleback (Gasterosteus Aculeatus), Saif Quraishi

Honors Scholar Theses

The study of fibrosis in stickleback has been a growing field in which there have been a few studies conducted. This study examines two experiments involving the inhibition of fibrosis in stickleback. The first experiment used fish from the Kenai River Flats (KRF) in Alaska. The second experiment used fish from Roselle Lake in British Columbia, Canada. To determine the impact the drug had on fibrosis, sticklebacks from the KRF population were treated with DB1976 as well as alum, a pro‐inflammatory vaccine adjuvant that causes fibrosis (Kool et. al 2012). When each DB1976 + alum treatment group was compared with …

Lifestyle Behaviors Before And After Diagnosis Of Type 2 Diabetes: Findings From The Nurses’ Health Studies, Amelia E. Roebuck

Dissertations and Theses (Open Access)

Short- and long-term changes in diet or physical activity in relation to type 2 diabetes mellitus (T2DM) and factors associated with behavior change with adequate inclusion of relevant confounding and effect-modifying variables have been minimally explored. The objective of this research was to examine changes in diet and physical activity following a diagnosis of T2DM and identify sociodemographic, medical, psychosocial, and lifestyle factors associated with changes in dietary or physical activity behaviors. This dissertation utilized two prospective cohorts of U.S. female nurses participating in the Nurses’ Health Study (1976-2016) and Nurses’ Health Study II (1989-2017). Linear mixed effects modeling was …

Usp1 Expression Driven By Ews::Fli1 Dually Promotes Ewing Sarcoma Cell Survival By Mitigating The Replication Stress Response And Stabilizing Survivin, Halle Mallard

Theses & Dissertations

Ewing sarcoma (EWS) is a malignant pediatric bone cancer. EWS express a fusion oncogene resulting from the reciprocal translocation of EWSR1 an ETS transcription factor. In most EWS tumors, this occurs with FLI1, creating the EWS::FLI1 fusion oncogene which encodes an aberrant transcription factor. Independent of its transcriptional activity, EWS::FLI1 deregulates other cellular processes driving oncogenesis. For cases with a single, localized tumor, roughly 80% of patients remain event-free after five years. Disease dissemination is a problem – about 25% of patients have metastatic disease at diagnosis. Long-term survival for these patients is less than 40%. Presently, no second line …

Paper-Based Ratiometric Sensor Array For Pathogenic Bacterial Species Identification, Aayushi Laliwala

Theses & Dissertations

Bacterial infections are the leading cause of mortality worldwide due to the lack of accurate diagnosis of the pathogens causing infections. Currently, microbiological and molecular techniques are used to identify pathogenic bacteria. However, these techniques have limitations as they are time-consuming, cost-inefficient, and require trained personnel. Therefore, timely and precise identification of pathogenic bacteria is crucial for public safety. Our lab previously synthesized a fluorescent sensor array (3-hydroxyflavone derivatives) encapsulated in hyaluronic acid-based nanoparticles to identify bacterial species and recognize their Gram status. Though promising, this technique was relatively time-consuming and required high sample and volumes.

To overcome these limitations, …

Cardiac Vagal Dysfunction And Ventricular Arrhythmogenesis In Type 2 Diabetes Mellitus After Myocardial Infarction, Dongze Zhang

Theses & Dissertations

Diabetes is the eighth leading cause of death in the United States. Every eight seconds a person dies from diabetes around the world. Among various types of diabetes, type 2 diabetes mellitus (T2DM) is the most common form, representing 90% to 95% of all diabetes populations. The leading cause of mortality and morbidity in T2DM patients is cardiovascular disease, among which acute myocardial infarction (MI)-induced ventricular arrhythmia is the major cause of death in T2DM. Patients with T2DM are two to four times more likely to die from MI than non-diabetic patients. Although well-known therapies including better glycemic control have …

Targeting Metabolic Switches And Dna Damage Repair For The Treatment Of Human Diseases, Rasangi Chathurika Kankanam Pathirage

Theses & Dissertations

Tuberculosis and Cancer treatments share common challenges due to prolonged therapy, the necessity of combination therapy and severe side effects. Characterization of novel drug targets and identification of new drugs modulating such targets within the context of multidrug treatments will contribute to overcoming current therapeutic challenges.

Tuberculosis (TB) is one of the challenging diseases to treat due to the persistence of Mycobacterium tuberculosis (Mtb) in the dormant phase. Current TB drugs are more effective toward actively dividing bacteria and less effective toward dormant bacteria. The metabolic transition between actively dividing bacteria and dormant bacteria is driven by pivotal metabolic enzymes …

The Effect Of Non-Immersive Virtual Reality Radiographic Positioning Simulation On First-Year Radiography Students' Image Evaluation Of Routine Positioning Errors, Ellie M. Miller

Theses & Dissertations

Optimal radiographic image quality is critical because it affects the accuracy of the radiologist’s interpretation. Radiologic technologists are obligated to obtain diagnostic images while minimizing patient radiation. However, repeated images due to technologist error have increased in recent years. This study aimed to evaluate the effectiveness of non-immersive virtual reality (VR) simulation on first-year students’ image evaluation of common positioning errors on routine knee and lumbar spine protocols. Research questions were 1) Does guided instruction using non-immersive radiographic positioning simulation software lead to improved image evaluation scores? 2) How well do participants describe evaluation of positioning errors after guided instruction …

Biomechanics Of The Tip-Back Bend: An Analysis Of The Force System Via Translational Research, Lyndzie Brace

Theses & Dissertations

Purpose: The purpose of this study was to 1. experimentally (in vitro) quantify the force system acting on the molar and the incisor when a tip-back bend is placed on an auxiliary spring mounted on a base archwire and create a prediction model 2. to compare the type of tooth movement obtained from the prediction model (in-vitro) and the clinical outcome (in-vivo) data obtained from a previously conducted clinical trial. Methods: A computerized 3-dimensional (3D) orthodontic force tester (OFT) was used to quantify and compare the force systems generated from different combinations of tip-back bend archwires and base …

Early Diagnosis Methods For Autism Spectrum Disorder: A Systematic Review, Megan Denise Mccarthy

Doctoral Dissertations

Objective: This systematic review aimed to comprehensively synthesize existing literature on early detection methods for Autism Spectrum Disorder (ASD) in children birth to 3 years, that lead to an effective and reliable early diagnosis in children 2 to 3 years of age.

Methods: A systematic search was conducted across multiple electronic databases, including PubMed, Scopus, and PsycINFO following PRISMA guidelines. Studies reporting on early detection methods for ASD in infants and toddlers within ages 0 to 3 years that lead to early diagnosis of children ages 2 to 3 years were eligible for inclusion. Data extraction and quality assessment were …

Advances In Diagnostic Tools And Therapeutic Approaches For Gliomas: A Comprehensive Review, Gayathree Thenuwara Nanolab Research Centre, James Curtin, Furong Tian Nanolab Research Centre

Articles

Gliomas, a prevalent category of primary malignant brain tumors, pose formidable clinical challenges due to their invasive nature and limited treatment options. The current therapeutic landscape for gliomas is constrained by a “one-size-fits-all” paradigm, significantly restricting treatment efficacy. Despite the implementation of multimodal therapeutic strategies, survival rates remain disheartening. The conventional treatment approach, involving surgical resection, radiation, and chemotherapy, grapples with substantial limitations, particularly in addressing the invasive nature of gliomas. Conventional diagnostic tools, including computed tomography (CT), magnetic resonance imaging (MRI), and positron emission tomography (PET), play pivotal roles in outlining tumor characteristics. However, they face limitations, such as …

Characterization Of Trafficking And Metabolic Functions Of The Endocytic Regulator Ehd1 In Ewing Sarcoma, Sukanya Chakraborty

Theses & Dissertations

Ewing sarcoma (EWS) is the second most common bone malignancy in children and adolescents. Despite improvement in survival due to advances in multimodality treatment strategies, patients with metastatic or recurrent disease have very poor outcomes. Overexpression of the EPS15 Homology Domain containing 1 (EHD1) protein has been linked to tumorigenesis but whether its core function as a regulator of intracellular traffic of cell surface receptors plays a role in oncogenesis remains unknown.

Studies presented in this dissertation establish that EHD1 overexpression is a feature of nearly 90% EWS patient tumors with high EHD1 expression specifying shorter patient survival. ShRNA-knockdown and …

Three-Dimensional Analysis Of Condylar Displacement After Orthognathic Surgery In Patients With Facial Asymmetry, Teresa Bae

Theses & Dissertations

Facial asymmetry is apparent when there is a clinically significant variation between the two halves of the face. There can be varying degrees of facial asymmetry and mild asymmetry can be treated by orthodontics alone. For severe facial asymmetry a combination of orthodontics and orthognathic surgery may be necessary. The condyle must be in a stable position following surgery to have functional and structural stability and prevent relapse. This research proposal is to perform a retrospective CBCT study on the displacement of the condyle following orthognathic surgery in patients with facial asymmetry. The CBCTs were obtained from Taipei Veterans General …

Internet Generations In The Labor Market, Grażyna Wieczorkowska, Kinga Wilczyńska

Studia i Materiały Wydział Zarządzania Uniwersytet Warszawski

Objectives: When describing generational differences, the concept of a collective (prototypical) unit is used, which has been shaped by prevalent social and cultural conditions during the formative period of secondary socialization. Generations whose privileged period coincided with the widespread use of the internet have been referred to as internet generations. The article aims to draw attention to the need to separate the effects of biological age (younger doroworkers differ from older ones in terms of factors such as muscle strength and fresh memory) from the effects of generation resulting from environmental differences during the privileged period of secondary socialization. Methodology: …

Interactive Effects Of Sublethal Concentrations Of Fracking Biocides And Abandoned Mine Drainage On Amphipod Behavior, Kelly Lenhart

Electronic Theses and Dissertations

This thesis examined the sublethal effects of three pollutants, namely glutaraldehyde, 4,4-dimethyloxazolidine (DMO), and abandoned mine drainage (AMD), on amphipods. The primary objective was to investigate their combined effects on amphipods. The three pollutants, despite having the potential to combine in the environment, have not been studied to determine their potential for detrimental interactive effects which could result in unexpected environmental damage.

The research employed a series of experimental setups involving controlled exposure of amphipods to varying, putatively sublethal, concentrations of the chemicals of interest. Subsequently, effects were assessed via both behavioral and feeding assessments. To facilitate this analysis, novel …

Ascot App, Milla Penelope Markovic

Honors Thesis

The Ascot App is a research tool for acquiring and analyzing data. The app comprises of both mobile and web platforms, each serving a unique purpose. The mobile side allows users to input data through the app’s form, which is uploaded to a database for further processing and analysis. The web app, which is still under development as of April of 2024, allows users to manage their research project and download data in the form of a parsed CSV. These components ensure a seamless process for research teams to record data with persistence and security while allowing for analysis.

Ascot …

Enhancing The Anticancer Potential Of Metformin: Fabrication Of Efficient Nanospanlastics, In Vitro Cytotoxic Studies On Hep-2 Cells And Reactome Enhanced Pathway Analysis, Shereen Nader, Sara El Wahed, Noha Badawy, Mona Saber, Maha Abdollah

Pharmacy

Metformin (MET), an oral antidiabetic drug, was reported to possess promising anticancer effects. We hypothesized that MET encapsulation in unique nanospanlastics would enhance its anticancer potential against HEP-2 cells. Our results showed the successful fabrication of Nano-MET spanlastics (d = 232.10 ± 0.20 nm; PDI = 0.25 ± 0.11; zeta potential = (−) 44.50 ± 0.96; drug content = 99.90 ± 0.11 and entrapment efficiency = 88.01 ± 2.50%). MTT assay revealed the enhanced Nano-MET cytotoxicity over MET with a calculated IC50 of 50 μg/mL and > 500 μg/mL, respectively. Annexin V/PI apoptosis assay showed that Nano-MET significantly decreased …

Functionalized Surface Of Plga Nanoparticles In Thermosensitive Gel To Enhance The Efficacy Of Antibiotics Against Antibiotic Resistant Infections In Endodontics: A Randomized Clinical Trial, Mona Arafa, Hadeel Moussa, Mohamed Medhat Kataia

Pharmacy

Enterococcus faecalis plays the key role in endodontic infections and is responsible for the formation of biofilm on dentin, which causes a resistance against periradicular lesions treatment, consequently the aim of this study is to use nanoparticles entrapping anibacterial agents coated with chitosan that in authors previous study showed a successful in vitro biofilm inhibition, additionally incorporated in thermoresponsive gel.to benefit nanoparticles` small size, and the positive charge of their surfaces that binds with the negatively charged surface of bacterial cell causing its destruction, in addition to the sustained release pattern of the drug based nanoparticles in gel. Therefore, Ciprofloxacin …

Protocol For An Agent-Based Model Of Recombination In Bacteria Playing A Public Goods Game, Isaiah Paolo A. Lee, Omar T. Eldakar, J. Peter Gogarten, Cheryl P. Andam

Biology Faculty Articles

Agent-based models are composed of individual agents coded for traits, such as cooperation and cheating, that interact in a virtual world based on defined rules. Here, we describe the use of an agent-based model of homologous recombination in bacteria playing a public goods game. We describe steps for software installation, setting model parameters, running and testing models, and visualization and statistical analysis. This protocol is useful in analyses of horizontal gene transfer, bacterial sociobiology, and game theory.

For complete details on the use and execution of this protocol, please refer to Lee et al.¹

Engaging With ‘Less Affluent’ Communities For Food System Transformation: A Community Food Researcher Model (Foodsequal Project), C. Pettinger, L. Hunt, H. Gardiner, P. Garg, L. Howard, C. Wagstaff

School of Health Professions

The UK food system is distorted by inequalities in access, failing the people most in need, yet it should provide access to safe, nutritious affordable food for all citizens. Dietary patterns are associated with sociodemographic characteristics, with high levels of diet-related disease mortality attributed to poor dietary habits. Disadvantaged UK communities face urgent public health challenges, yet area often treated as powerless recipients of dietary and health initiatives. The need for food system transformation has been illustrated within recent UK government policy drivers and research funding. The Food Systems Equality project1 is a research consortium that aims to ‘co-produce healthy …

A Virtual Reality Conditioned Place Preference In Undergraduates With Nicotine Use, Lukas Klin

Honors Scholar Theses

This study was conducted to determine if a Conditioned Place Preference (CPP) could be established for a Virtual Reality (VR) environment that was previously paired with virtual nicotine stimuli. 254 undergraduate students with varying levels of nicotine use from the University of Connecticut were recruited to participate in the study. Participants completed six, three-minute conditioning sessions where they were confined to one of two VR rooms with one room containing virtual nicotine stimuli while the other was neutral. Participants then completed a three-minute test session, in which they were allowed access to both rooms and there were no virtual nicotine …

Author’S Response To Ang, O. Invited Commentary On The Development And Initial Validation Of The Pain Progress Measure, Adnan N. Balisi

Philippine Journal of Physical Therapy

No abstract provided.

Interrogation Of The Molecular Mechanisms That Finetune Fcεri Signaling And Mast Cell Outcomes, Derek A. Rinaldi

Biomedical Sciences ETDs

FcεRI is the ITAM receptor primarily responsible for the allergic response and immune hypersensitivity diseases such as asthma. Regulation of the strength of both FcεRI signaling, and the resulting outcomes, is accomplished via a complex array of molecular mechanisms whose interactions remain unclear. Here, I explored how the biophysical properties of antigens, crosstalk between FcεRI and integrin signaling and Arf6-mediated control of trafficking and exocytosis tune mast cell outcomes. I first found significant differences in FcεRI aggregation, phosphorylation and granule secretion after crosslinking with the multivalent antigen DNP-BSA or the small, trivalent antigen DF3. I also revealed a bi-directional, Syk-mediated …

Best Practices To Manage Nitrate Contamination Of Groundwater In Agricultural Zones: A Comparative Analysis Of Farming Impacts On Areas Of Central Valley And High Plains Aquifers, Rekha Duthulur

Master's Projects and Capstones

Nitrate is one of the most common contaminants in groundwater and causes multiple health impacts when consumed. Contamination is most significant in agricultural areas and has increased steadily since the 1950s with the introduction of nitrogen fertilizers. The depth of an aquifer, sediment type, hydrogeology, redox conditions, microbe activity, fertilizer application, natural nitrogen deposits, and well construction influence the degree of nitrate contamination of an aquifer. The sources of nitrate pollution are fertilizer, manure, concentrated feeding lots, natural nitrogen deposits, atmospheric deposition, septic systems, wastewater treatment plants, and industrial activities. Nitrogen compounds applied on the surface are stored in the …

Solar-Powered Microgrids In Northern California: An Opportunity For Resilience, Marina Riddle

Master's Projects and Capstones

Planned and unplanned power outages have been increasing in frequency and duration, negatively impacting all public sectors, and threatening public safety. These outages are deadly to those who rely on medical devices. As climate change-fueled extreme weather events (wildfires, earthquakes, storms, etc.) also increase in frequency, our electrical grid must be prepared to bounce back. Microgrids provide necessary redundancy and reliability. Through a novel GIS suitability analysis, based on solar radiation, land use type, local energy demand, distance to transmission lines, distance to roads, and slope, optimal locations for solar-powered microgrids throughout Northern California were determined. The counties of Fresno, …

Association Of Covid-19 Coinfection With Increased Mortality Among Patients With Pseudomonas Aeruginosa Bloodstream Infection In The Veterans Health Administration System, Leila S. Hojat, Brigid M. Wilson, Federico Perez, Maria F. Mojica, Mendel E. Singer, Robert A. Bonomo

Faculty Scholarship

Objective: Pseudomonas aeruginosa bloodstream infection (PA-BSI) and COVID-19 are independently associated with high mortality. We sought to demonstrate the impact of COVID-19 coinfection on patients with PA-BSI. Design: Retrospective cohort study. Setting: Veterans Health Administration. Patients: Hospitalized patients with PA-BSI in pre-COVID-19 (January 2009 to December 2019) and COVID-19 (January 2020 to June 2022) periods. Patients in the COVID-19 period were further stratified by the presence or absence of concomitant COVID-19 infection. Methods: We characterized trends in resistance, treatment, and mortality over the study period. Multivariable logistic regression and modified Poisson analyses were used to determine the association between COVID-19 …

Pkcε Activator Protects Hippocampal Microvascular Disruption And Memory Defect In 3×Tg-Alzheimer's Disease Mice With Cerebral Microinfarcts, Huaixing Wang, Zongxiu Zhang, Jarin Hongpaisan

Center for Translational Medicine Faculty Papers

BACKGROUND: Current evidence suggests that microvessel disease is involved in Alzheimer's disease (AD). Cerebrovascular disease correlates with cardiovascular disease and is complicated in ≈40% of AD patients. The protein kinase C (PKC) ε activator DCPLA can stimulate human antigen (Hu) R that prevents degradation and promotes the translation of mitochondrial Mn-superoxide dismutase (MnSOD) and vascular endothelial growth factor-A (VEGF) mRNAs.

METHODS: To induce brain microinfarcts, we injected triple transgenic (3×Tg) and wild-type (WT) control mice with microbeads (20 μm caliber) into common carotid arteries, with or without the DCPLA-ME (methyl-ester) for 2 weeks. After water maze training, mice at 16 …

Unsupervised Multi-Resolution Analysis Of Protein-Protein Abundance Patterns In Infectious Exacerbation Of Copd, Eric Everingham

Theses

Despite consistently appearing as a top 5 cause of death domestically and internationally, understandings of the underlying biological interactions of COPD remain insufficient. Particularly, early identification of highly vulnerable patients that may experience more severe infectious exacerbation is a challenge during diagnostics. This project leverages unsupervised clustering and multi-resolution decomposition of proteomic abundance profiles in a small cohort of COPD subjects to identify candidate biomarkers of heightened exacerbation risk. As part of an ongoing study, serum and sputum samples were collected during well visits (stable illness) from n=67 subjects, and analyzed using quantitative proteomics. A scores principal component analysis was …