Digital Commons Network^™

Density And Abundance Estimation Of West Indian Manatee, Trichechus Manatus, Between The States Of Ceará And Piauí, Northeast Brazil, Using Active Acoustics, Katherine Fiedler Choi Lima, Denis Moledo De Sousa Abessa, Daniel Gonzalez-Socoloske, Renata Sousa-Lima, Ana Carolina Oliveira De Meirelles, Cristine Pereira Negrão Silva

Faculty Publications

The West Indian manatee is one of the most threatened species in Brazil. The species has currently a patchy distribution from the state of Alagoas to the state of Amapá. The difficulty of observing manatees, especially in estuarine waters, is a challenge for conservation. Therefore, it is necessary to use new methodologies and technologies to solve manatee detection problems in their natural habitats. The goal of this study was to use an active acoustic method of detection to estimate manatee density and abundance in the estuarine complex of the Timonha and Ubatuba rivers, between the states of Ceará and Piauí, …

The Sense Of Safety Theoretical Framework: A Trauma-Informed And Healing-Oriented Approach For Whole Person Care, Kurt C. Stange

Faculty Scholarship

Objectives: This research describes four aspects of the development of the Sense of Safety Theoretical Framework for whole person care: exploring the meaning of the phrase “sense of safety”—the whole person language; the range of human experience that impacts sense of safety—whole person scope; the dynamics that build sense of safety—the healing goals; and the personal and cross-disciplinary trauma-informed practitioner skills and attitudes that facilitate sense of safety. Methods: This qualitative participatory study was conducted in two phases. Researchers iteratively explored the concept of sense of safety using focus groups and semi-structured interviews. Overarching research questions were: “Does the transdisciplinary …

Outcomes For Patients With Head And Neck Sarcoma Treated Curatively With Radiation Therapy And Surgery, Alison K Yoder, Ahsan Farooqi, Devarati Mitra, J Andrew Livingston, Dejka M Araujo, Erich M Sturgis, Ryan Goepfert, Andrew J Bishop, B Ashleigh Guadagnolo

Faculty, Staff and Student Publications

PURPOSE: Soft tissue sarcomas (STSs) of the head and neck (H&N) are rare malignancies that are challenging to manage. We sought to describe the outcomes of patients treated with curative intent using combined surgery and radiation therapy (RT) for H&N STS.

METHODS AND MATERIALS: We performed a single-institution retrospective review of patients with nonmetastatic STS of the H&N who were treated from 1968 to 2020. The Kaplan-Meier method was used to estimate disease-specific survival (DSS) and local control (LC). Multivariable analyses (MVAs) were conducted using Cox proportional hazards model.

RESULTS: One hundred ninety-two patients had a median follow-up of 82 …

Improving Growth Models Of Cultivated Sugar Kelp, Saccharina Latissima, By Accounting For Intraspecific Variation In Thermal Tolerance, Ruby Krasnow, Sara Gonzalez, Scott Lindell

Student Publications [Scholarly]

Dynamic models of sugar kelp (Saccharina latissima) growth are used to estimate the production potential of seaweed aquaculture in many regions around the world. These models do not currently account for the existence of S. latissima ecotypes that are adapted to regional environmental conditions, particularly temperature. We tested the hypothesis that recalibrating the temperature parameters of a dynamic energy budget model using literature data for S. latissima from regions with a similar climate to the region of interest would result in more accurate predictions than using a general species-wide temperature response curve. Calibrating the model using data from warm regions …

A Simple, Inexpensive Alkaline Method For Bacterial Dna Extraction From Environmental Samples For Pcr Surveillance And Microbiome Analyses, Abdulkarim Shwani, Bin Zuo, Adnan Alrubaye, Jiangchao Zhao, Douglas D. Rhoads

Poultry Science Faculty Publications and Presentations

DNA extraction for downstream molecular diagnostic applications can be an expensive, time-consuming process. We devised a method to quickly extract total bacterial DNA from environmental samples based on the sodium hydroxide lysis of cells with or without capture by magnetic beads for subsequent PCR or quantitative PCR. The final DNA extraction method using NaOH is extremely low-cost and can be completed in as little as 10 min at room temperature with dilution, or the DNA can be further purified using silica-coated paramagnetic beads. NaOH extraction was effective for Gram-negative and Gram-positive bacteria in samples from air, soil, sewage, food, laboratory …

Next Generation Microfluidics: Fulfilling The Promise Of Lab-On-A-Chip Technologies, Umut A. Gurkan

Faculty Scholarship

Microfluidic lab-on-a-chip technologies enable the analysis and manipulation of small fluid volumes and particles at small scales and the control of fluid flow and transport processes at the microscale, leading to the development of new methods to address a broad range of scientific and medical challenges. Microfluidic and lab-on-a-chip technologies have made a noteworthy impact in basic, preclinical, and clinical research, especially in hematology and vascular biology due to the inherent ability of microfluidics to mimic physiologic flow conditions in blood vessels and capillaries. With the potential to significantly impact translational research and clinical diagnostics, technical issues and incentive mismatches …

Fabrication And Validation Of Sub-Cellular Carbon Fiber Electrodes, Yu Huan, Hillel J. Chiel

Faculty Scholarship

Multielectrode arrays for interfacing with neurons are of great interest for a wide range of medical applications. However, current electrodes cause damage over time. Ultra small carbon fibers help to address issues but controlling the electrode site geometry is difficult. Here we propose a methodology to create small, pointed fiber electrodes (SPFe). We compare the SPFe to previously made blowtorched fibers in characterization. The SPFe result in small site sizes (105.4 ± 20.8μm²) with consistently sharp points (20.8 ± 7.64°). Additionally, these electrodes were able to record and/or stimulate neurons multiple animal models including rat cortex, mouse retina, …

Cerebellar Feedback Effects On Responses To Conspecific Signals Of Different Frequencies In The Electrosensory System, Elora K. Shinn

Graduate Theses, Dissertations, and Problem Reports (ETD)

Sensory systems in animals have evolved to translate physical stimuli into neural representations that, in turn, guide behavior. Feedback is critical in these processes, allowing organisms to filter redundant information and respond effectively to relevant stimuli. In humans, for example, the cerebellar flocculus provides feedback to the vestibulo-ocular reflex to stabilize eye movements during body rotation. In bats, the feedback during echolocation helps prioritize essential signals from the environment. In weakly electric fish, cerebellar feedback helps filter out redundant low-frequency modulations caused by conspecifics, enabling them to detect prey and other relevant signals. However, the influence of cerebellar feedback on …

The Flora, Fauna, And Paleoenvironment Of The Late Middle Miocene Quebrada Honda Basin, Bolivia (Eastern Cordillera, Central Andes), Beverly Z. Saylor, Russell K. Engelman, Darin A. Croft

Faculty Scholarship

Miocene ecosystem change in the Central Andes is not well understood because of a dearth of well-dated fossil sites from the region. The late Middle Miocene (~13-12 Ma) Quebrada Honda Basin (QHB) in southern Bolivia (22° S) helps fill this gap and provide vital insights into Neotropical paleoenvironments. The site is among the best-characterized Middle Miocene terrestrial vertebrate sites of South America and has a robust temporal, spatial, and lithostratigraphic framework for analyzing its sedimentary facies, fossils, and paleoenvironment. Here, we present new plant silica (phytolith) assemblage data from the QHB as well as new analyses of QHB faunal data. …

Investigation Of The Ecological Interactions And Host Specificity Of Verticillium Nonalfalfae, A Proposed Biological Control Agent Against The Invasive Tree-Of-Heaven (Ailanthus Altissima), Kristen H. Pierce

Graduate Theses, Dissertations, and Problem Reports (ETD)

Tree-of-heaven (Ailanthus altissima) is a highly invasive tree species with a cosmopolitan distribution, including an expansive range in North America. In the U.S., tree-of-heaven has established populations in more than 40 states and is considered among the most notorious of invasive plant species. The discovery of Verticillium nonalfalfae and V. dahliae causing acute wilt and mortality of Ailanthus in the mid-Atlantic in the 2000s has resulted in numerous studies evaluating the utility of these fungi as potential biocontrol agents. Recent efforts to commercialize V. nonalfalfae have raised additional questions about its widespread use and factors influencing long-term efficacy. …

Appropriate Treatment Options For Depression And Anxiety For Patients Who Use Cannabis, Christina J. Esmon

DNP Projects

An estimated 431,000 adults in Mississippi (MS) have a mental health condition (Duckworth, 2021). In January 2022, MS legislation passed the MS Medical Cannabis Act (Mississippi Legislature Regular Session, 2022). With over 22,000 participants in the program as of September 2023. providers need to be equipped with the tools and resources necessary and available to provide adequate care for those patients who use cannabis. Review of literature indicated an increase in the number of people with anxiety and depression, and an increase in the use of cannabis in this population. This quality improvement project’s purpose was to establish adequate and …

Development Of A New Green Infrastructure By Using Biochar Amended Topsoil For On-Site Stormwater Runoff Treatment, Ayesha Sadia

College of Graduate Studies: Theses & Dissertations

This Research Project aimed to design, build, operate and maintain, and monitor a new green infrastructure (GI) using biochar-amended topsoil for on-site stormwater runoff treatment in urban areas. The project involved evaluating the removal efficiencies of pollutants from stormwater runoff such as total suspended solid (TSS), nutrients including nitrogen and phosphorous, heavy metals including lead (Pb), zinc (Zn) and copper (Cu) and oil and grease etc. from the media of biochar amended topsoil. In this Project, a full-scale Field Test Site with a total area of 500 and 1 ft depth was built to on-site treat stormwater runoff from a …

Psychedelic Expectancies And Post-Traumatic Stress Disorder Symptoms, Samantha Gomez

Electronic Theses & Dissertations (2024 - present)

Millions of Americans struggle with posttraumatic stress disorder (PTSD) every year. Though typical treatments work for some, patients often drop out of treatment. Given that patients are often retelling their traumatic experiences, or confronting stimuli and situations that are relevant to the experience, psychotherapy for PTSD can be psychologically demanding and intensive. These factors provide a further understanding for the high rates of PTSD treatment attrition. Psychedelic assisted psychotherapy (PAP) is a potential option to mitigate these concerns. Preliminary research on PAP has revealed effective and promising results. However, questions about PAP and their public perception remain. Furthermore, treatment expectancies, …

The Contextual Process Of Bystander Intervention In Bias-Motivated Violent Victimization: An Experimental Approach, James Hubbell

Electronic Theses & Dissertations (2024 - present)

Hate crimes against Asian Americans and LGBT individuals have been on the rise within the U.S. in the past few years (UCR, 2024). Although these offenses tend to occur in public, numerous stories in the media highlight that few bystanders choose to intervene. To unveil why bystanders choose not to intervene, this dissertation explored the extent to which 1,001 U.S. adults engaged with the five-step situational model for bystander intervention proposed by Latané and Darley (1970). Respondents were recruited through Qualtrics and randomly assigned to a hate scenario involving one of six victims: an Asian American man, an Asian American …

Assessing Associations Between Emergency Room Visits For Respiratory And Cardiovascular Diseases And Criteria Air Pollutants Exposure In New York State, Tamba Solomon Lebbie

Electronic Theses & Dissertations (2024 - present)

Abstract

Air pollution is a major environmental health hazard for the general population. Approximately 90% of the global population is at risk of both indoor and outdoor air pollution, and it accounts for an estimated 7 million premature deaths every year according to the World Health Organization (WHO). Multiple studies including epidemiological and molecular analyses have confirmed the negative effects of air pollutants on human health even at levels within the WHO guidelines. This study examines the health impacts of releases of criteria air pollution from point sources in New York State (NYS). There is evidence from population studies that …

Ribosome Hibernation Factor Induces Antibiotic Persisters Of Mycobacterium Tuberculosis In Neutrophil-Rich Lung Lesions, Jamie Corro

Electronic Theses & Dissertations (2024 - present)

Mycobacterium tuberculosis (Mtb), the etiological agent of tuberculosis, is a prescient global health threat and the leading cause of death by an infectious agent. Treatment of Mtb infection involves administering 2-4 antibiotics for a minimum of 6 months. This extended drug regimen is required to target a subpopulation of persistent bacilli. As their name implies, these bacteria can “persist” by acquiring nonhereditary and transient antibiotic tolerance. Although the precise mechanism is unknown, it’s often attributed to slowing cellular growth and metabolic processes. During zinc deprivation, bacteria can remodel their ribosomes by replacing their zinc-binding paralogues containing the CXXC motif (C+) …

Remediating Fibrosis To Restore Salivary Gland Function, Jennifer Morrissey

Electronic Theses & Dissertations (2024 - present)

Fibrosis is defined by the overgrowth, hardening, and scarring of various tissues and is attributed to excess deposition of extracellular matrix components including collagen. In the case of persistent injury or autoimmunity, as in Sjogren’s Disease, the ECM persists. Fibrosis can be studied in multiple animal models. Our lab has published the use of a mouse submandibular salivary gland partial resection model. In this procedure 40% of the left distal tip of the submandibular salivary gland (SMG) is excised. This leads to a chronic sustained fibrotic response in a localized region. The use of the non-obese diabetic mouse (NOD) has …

Effects Of Grant Termination For Orphans, Vulnerable Children And Their Caregivers In Windhoek, Namibia, Miriam Winnie Hasheela

Electronic Theses & Dissertations (2024 - present)

Namibia faces complex child welfare policy dilemmas in providing sustainable support for the increasing number of AIDS-orphaned youth transitioning into adulthood. While Orphan and Vulnerable Children (OVC) social grants have played a vital role in poverty alleviation and childhood development (Ministry of Gender Equality, Poverty Eradication and Social Welfare, 2021), the abrupt termination of these grants for youth when they reach 18 years of age without any transitional support has posed significant risks to their well-being. While much research in Namibia (Mnubi-Mchombu and Ocholla, 2014; Van Der Brug, 2012.) has examined the impact of OVC grants on children under the …

Compassion Cartography: Mapping The Psychological Landscape Of Altruistic Equity And Effectiveness, Kyle Fiore Law

Electronic Theses & Dissertations (2024 - present)

Psychologists and philosophers have often championed deliberative reasoning over empathy as a better approach to overcoming parochial biases and guiding altruistic equity and effectiveness. Advocates of the effective altruism (EA) philosophy even advise philanthropists to downregulate empathic responses to maximize the impact of their donations. However, recent research reveals that extraordinary altruists (XAs), such as those who donate organs to strangers, are driven primarily by empathy. This dissertation explores whether empathy necessarily impedes altruistic equity (impartial regard for others’ welfare) and effectiveness (prioritizing impact), revealing a more nuanced reality that challenges this perspective. Across two phases of research involving three …

Implications Of 5-Methylcytosine (M5c) And N4-Actylcytidine (Ac4c) In Epithelial To Mesenchymal Transition (Emt) In Human Breast Cancer, Manuel A. Pazos Ii

Electronic Theses & Dissertations (2024 - present)

Breast cancer is a complex disease that continues to haunt women and their loved ones around the world. It is currently the leading cause of cancer-related mortality among women. While most breast cancer patients can catch the disease before progression, others are less fortunate. The prognosis of those whose disease has advanced to metastatic or invasive breast cancer (IBC) is grim. Metastasis occurs when cancer cells undergo epithelial to mesenchymal transition (EMT), migrate to distant body tissues, then form new tumors. It is imperative to better understand the process of EMT to develop superior ways to detect breast cancer before …

Stabilizing Bulk Lead-Free Tin Halide Perovskites, William Rice

Electronic Theses & Dissertations (2024 - present)

Tin halide perovskites are a growing candidate in the semiconducting materials field due to their band gap, and ability to be doped (specifically p-type doped). Halide perovskites are sensitive to light and air which causes the degradation of the material from its preferred cubic perovskite lattice. This thesis introduces a way of stabilizing bulk CsSnCl3 through the Goldschmidt tolerance factor. It will also introduce potential new ways to p-type dope halide perovskites. We attempted to p-type dope our halide perovskite by substitutional, interstitial, and vacancy doping to increase the carrier concentration to compete with current perovskites. During our trials of …

The Study Of Ribosome-Amplified Metabolism, Rambo, Effect In Vitro And In Live Cells, Jianchao Yu

Electronic Theses & Dissertations (2024 - present)

Quinary interactions arise due to the extreme macromolecular crowding inside live cells. Quinary interactions affect intracellular protein stability, activity, and homeostasis. Ribosomes, traditionally known for their role in protein synthesis, have been found to engage in quinary interactions with metabolic enzymes, modulating their activity and thereby revealing a potential regulatory role for ribosomes in cellular metabolism. This phenomenon, termed the Ribosome-Amplified MetaBOlism (RAMBO) effect, was investigated in this dissertation through a series of in vitro and in vivo studies using primarily Nuclear Magnetic Resonance (NMR) spectroscopy.

Firstly, an NMR-based direct enzyme assay compatible with ribosome was developed and validated using …

The Role Of Familial Support And Gender In Asian American Students’ Stem Career Development: A Test Of Social Cognitive Career Theory, Xiao Ting Wang

Electronic Theses & Dissertations (2024 - present)

This study, based on Social Cognitive Career Theory (SCCT; Lent et al., 1994, 2000), investigated the role of familial support in the STEM career development of Asian American college students. Participants included 446 first-year undergraduate students who identified as Asian or Asian American and had not declared an academic major. Due to multicollinearity, four initial familial support variables—instrumental assistance, career-related modeling, verbal encouragement, and emotional support—were regrouped into two: performance motivation (instrumental assistance and verbal encouragement) and career mentoring (career-related modeling and emotional support). Structural equation modeling showed acceptable fit for a modified model, and the results partially supported the …

Studies In Three-Component Modification Of Lysine, Investigating Reversible Potential Of An Intricate Bicyclo [3.3.1] Nonane Scaffold, Ryan Bartlett

Electronic Theses & Dissertations (2024 - present)

Synthesis of small-molecule conjugates capable of selective attachment to a peptide/protein backbone paves way for new therapeutic applications of protein modification. Sufficient inhibition or installation of function requires extensive design so the compound may identify and attach to the intended site. The utility of such an approach has prompted a number of applicable strategies and has rendered the development of new modification scaffolds less necessary. On the other hand, covalent modification to the biologics with ability to remove the conjugate via stimulus is underdeveloped, amplifying a current need to investigate the reversible potential for conjugation and deconjugation techniques in regard …

Evaluation Of The Physicochemical Properties And Soil Biogeochemistry Of Fire Residues, Lingqun Zeng

Electronic Theses & Dissertations (2024 - present)

Wildland fire is a main driving force in the Earth system and affects ecosystem properties and processes across spatio-temporal scales. The burning of biomass and production of solid fire residues (conventionally termed fire ash) that are a mixture of completely and incompletely burned materials (inorganic ash and pyrogenic carbon), represent the direct and prominent changes by fires. Cycling of the fire residues is an integral component of elemental cycles and can mediate other biogeochemical processes.

This dissertation aims to fill important knowledge gaps in understanding the disturbance of wildland fires to biogeochemical processes in soils. First, this work characterized and …

“A True Tower Of Babel”: Nation, Race, And Gender In The French Foreign Legion, 1831 To 1854, Nicholas Michael Charnley

Electronic Theses & Dissertations (2024 - present)

The French Foreign Legion, part of the July Monarchy’s 1831 expansion of the Army of Africa, sought to create a non-French force entirely deployable outside of the metropole. The creation of the expatriate corps intended to solve two of France’s most pressing problems at the time: an excess of foreign migrants at home, and a dearth of French soldiers abroad. The Paris government hoped to attract recruits from its considerable non-national male population, particularly political exiles and migrant workers fleeing Europe’s 1830 revolutionary wave. Initially intended as a temporary expedient for the homeland’s demographic crisis, the expatriate fighting force grew …

Outcome-Based Patient-Therapist Matching Implementation: A Study Of Clinician Beliefs And Attitudes, Ashleigh Smith

Electronic Theses & Dissertations (2024 - present)

Background. Implementation science aims to identify methods that support the adoption of evidence-based practices (EBPs), including measurement-based care (MBC) and MBC-informed patient-therapist matching. Examining clinician attitudes can elucidate potential barriers to implementing EBPs, and attitudinal differences may exist across groups of therapists. In the context of a patient-therapist matching implementation project, this multi-method study investigated the relationship between clinician characteristics, attitudes toward EBPs/MBC, and implementation climate. Method. A sample of N = 72 therapists completed measures of demographic and clinical characteristics, attitudes toward EBPs, perceptions of implementation climate, and attitudes toward monitoring and feedback. A subset of N …

A Study Of Mercury Speciation Analysis Of Blood Using Liquid Chromatography Inductively Coupled Plasma Mass Spectrometry For Human Biomonitoring And Clinical Applications, Emily Jean Pacer

Electronic Theses & Dissertations (2024 - present)

Mercury (Hg) exposure from environmental sources and consumer products presents a significant public health concern. The toxicity of methylmercury (MeHg) differs considerably from inorganic mercury (iHg), such that their separation and quantification in whole blood is helpful in identifying sources and possible pathways of exposure. Speciation methods provide a more detailed picture regarding human exposure to toxic mercury species and their effects on human health. This dissertation focuses on advanced analytical techniques for human biomonitoring and clinical diagnosis of Hg exposure. The primary aim of this research is to address important knowledge gaps and analytical challenges in studies of Hg …

The Guild House: A Mixed-Methods Case Study Of Game-Utilizing Information/Learning Design For Autonomy-Supportive Instruction In An Asynchronous Online Course, Sturdy Knight

Electronic Theses & Dissertations (2024 - present)

Gamification has emerged over the past ~10-12 years as a popular design approach to problems of motivation in online or blended learning, with a particular emphasis on distinguishing between extrinsic motivation driven by points, grades, class rank, etc, vs. intrinsic motivation driven by personal values and interest in the curriculum. Self-Determination Theory (SDT) forms the theoretical basis of much of this literature, yet the mediating constructs of learner self-regulation and autonomous vs. controlled regulatory styles, which describe the processes by which extrinsic motivators like game rewards or curriculum requirements are integrated with intrinsic goals, are under-addressed. This has led to …

Intrinsic Lipid Curvature And Bilayer Elasticity As Regulators Of Channel Function: A Comparative Single-Molecule Study, Mohammad Ashrafuzzaman, Roger E. Koeppe Ii, Olaf S. Andersen

Chemistry & Biochemistry Faculty Publications and Presentations

Perturbations in bilayer material properties (thickness, lipid intrinsic curvature and elastic moduli) modulate the free energy difference between different membrane protein conformations, thereby leading to changes in the conformational preferences of bilayer-spanning proteins. To further explore the relative importance of curvature and elasticity in determining the changes in bilayer properties that underlie the modulation of channel function, we investigated how the micelle-forming amphiphiles Triton X-100, reduced Triton X-100 and the H_II lipid phase promoter capsaicin modulate the function of alamethicin and gramicidin channels. Whether the amphiphile-induced changes in intrinsic curvature were negative or positive, amphiphile addition increased gramicidin channel …