Digital Commons Network^™

Advancing Small Molecule Therapies For Myotonic Dystrophy Type 1, Sawyer M. Hicks

Electronic Theses & Dissertations (2024 - present)

Myotonic dystrophy type 1 (DM1) is a multisystemic disorder caused by the expression of expanded CUG (CUG^exp) repeat RNA from the myotonic dystrophy protein kinase (DMPK) gene. This CUG^exp repeat RNA’s gain-of-function mechanism leads to the sequestration of muscleblind-like (MBNL) proteins, resulting in widespread alternative splicing dysregulation across tissues. Despite significant research, there are currently no approved therapeutics targeting the underlying causes of DM1. This dissertation explores the development and optimization of a novel class of small molecules, Modified Polycyclic Compounds (MPCs), designed to address splicing dysregulation in DM1. MPCs were designed from previously published …

A Study Of Mercury Speciation Analysis Of Blood Using Liquid Chromatography Inductively Coupled Plasma Mass Spectrometry For Human Biomonitoring And Clinical Applications, Emily Jean Pacer

Electronic Theses & Dissertations (2024 - present)

Mercury (Hg) exposure from environmental sources and consumer products presents a significant public health concern. The toxicity of methylmercury (MeHg) differs considerably from inorganic mercury (iHg), such that their separation and quantification in whole blood is helpful in identifying sources and possible pathways of exposure. Speciation methods provide a more detailed picture regarding human exposure to toxic mercury species and their effects on human health. This dissertation focuses on advanced analytical techniques for human biomonitoring and clinical diagnosis of Hg exposure. The primary aim of this research is to address important knowledge gaps and analytical challenges in studies of Hg …

Stratifying The Social: The Race/Class Dialectic And The Fetishization Of Race In Our Neoliberal Contemporary, Steven D. Delmagori

Electronic Theses & Dissertations (2024 - present)

This dissertation utilizes the lens of Social Reproduction Theory to examine the consequences of viewing race and racism and other systems of oppression like Patriarchy as separate or additive autonomous ontologies. Intersectionality Theory posits oppressions as independent systems that “add” or “intersect” onto oppressed subjects in particular circumstances. On the other hand, responding to Intersectionality Theory, Social Reproduction Theory understands systems of oppression as mutually constitutive social relations. Social Reproduction Theory therefore allows for a more thorough understanding of the relationship between racism and other systems of oppression and their own relationship to capitalism. Rather than theorizing these social relations …

Removal Of Pfoa/Pfos In Water Using Electrocoagulation And Associated Life Cycle Impacts And Financial Costs, Tianhong Mu

Electronic Theses & Dissertations (2024 - present)

Electrocoagulation (EC) with a zinc anode demonstrated promising results to remove perfluorooctanoic acid (PFOA) and perfluorooctane sulfonate (PFOS) from an aqueous solution. However, the energy requirement for EC is usually very high due to water electrolysis or aeration. The second chapter of this dissertation aims to reduce energy consumption using an air-cathode in EC (ACEC) to supply oxygen electron acceptor without aeration for attenuating PFOA/PFOS in this new configuration. For the high PFOA concentration (0.25 mM), ACEC with 45 min of the reaction time exhibited an excellent PFOA removal (99.8 ± 0.3% removal) comparable to an EC with aeration (EC-aeration, …

Outcome-Based Patient-Therapist Matching Implementation: A Study Of Clinician Beliefs And Attitudes, Ashleigh Smith

Electronic Theses & Dissertations (2024 - present)

Background. Implementation science aims to identify methods that support the adoption of evidence-based practices (EBPs), including measurement-based care (MBC) and MBC-informed patient-therapist matching. Examining clinician attitudes can elucidate potential barriers to implementing EBPs, and attitudinal differences may exist across groups of therapists. In the context of a patient-therapist matching implementation project, this multi-method study investigated the relationship between clinician characteristics, attitudes toward EBPs/MBC, and implementation climate. Method. A sample of N = 72 therapists completed measures of demographic and clinical characteristics, attitudes toward EBPs, perceptions of implementation climate, and attitudes toward monitoring and feedback. A subset of N …

The Guild House: A Mixed-Methods Case Study Of Game-Utilizing Information/Learning Design For Autonomy-Supportive Instruction In An Asynchronous Online Course, Sturdy Knight

Electronic Theses & Dissertations (2024 - present)

Gamification has emerged over the past ~10-12 years as a popular design approach to problems of motivation in online or blended learning, with a particular emphasis on distinguishing between extrinsic motivation driven by points, grades, class rank, etc, vs. intrinsic motivation driven by personal values and interest in the curriculum. Self-Determination Theory (SDT) forms the theoretical basis of much of this literature, yet the mediating constructs of learner self-regulation and autonomous vs. controlled regulatory styles, which describe the processes by which extrinsic motivators like game rewards or curriculum requirements are integrated with intrinsic goals, are under-addressed. This has led to …

Transcription And Translation Regulation Of Germ Cell To Maternal Transition, Noor Kotb

Electronic Theses & Dissertations (2024 - present)

In sexually reproducing organisms, the process of gametogenesis produces mature eggs and sperms required for fertilization to occur. The egg's quality is crucial for the healthy development of the next generation, making oogenesis a tightly regulated process with multiple benchmarks. To investigate the intricacies of oogenesis, we utilized the Drosophila oogenesis system as our primary model. This choice was motivated by several factors. Firstly, the process of egg development in Drosophila is conserved across species, making it a valuable proxy for understanding oogenesis in other organisms, including humans. Secondly, Drosophila melanogaster has long been established as a powerhouse in genetic …

The Effects Of Human-Driven Land Use Change On American Alligator (Alligator Mississippiensis) Nesting Patterns, Madison Wallwork

UNF Graduate Theses and Dissertations

American alligators (Alligator mississippiensis) have received little scientific attention in the context of human-driven land use change. The goal of my study is to investigate how land use change may be affecting the spatial ecology of alligator nesting in Florida. Areas directly impacted by humans are growing rapidly in Florida, yet the impacts of this land use change on alligator populations and reproduction are largely unknown. Alligators are ecosystem engineers and apex predators, and their nests often double as nests for other reptilian species. Therefore, it is imperative that we understand how human-driven land use change may be impacting …

Investigating The Role Of Tesserae In Energy Absorption In Shark Cartilage, Molly Dobrow

UNF Graduate Theses and Dissertations

Unlike mammals, shark endoskeletons are composed of cartilage, which is less stiff compared to bone. Despite this structural disadvantage, sharks have risen to become apex predators, with reported maximum posterior bite force estimates approximating 5914 N in bull sharks. Shark jaws are estimated to undergo high cycle loading within the organism’s lifecycle without evidence of failure. Fatigue resistance may relate to mineral distribution within a component of the endoskeletal system – tesserae. Tesserae are thin, roughly hexagonal tiles composed of calcium phosphate hydroxyapatite (HA) crystals and grow by accretion to eventually surround the hyaline cartilage core. Though tesserae dimensions are …

Development Of Erosion Functions For Micp-Treated Beach Sand, Saeed Booshi

UNF Graduate Theses and Dissertations

This thesis investigates the effectiveness of microbially-induced calcium carbonate precipitation (MICP) using the recently introduced bioslurry treatment method via surface percolation. To simulate realistic water forcing conditions, a piston-style erosion rate testing apparatus, specifically the Florida Department of Transportation Sediment Erosion Rate Flume, was used. Initial tests conducted at a high shear stress of 42 Pa revealed that a combination of 15% bioslurry and 85% cementation solution exhibited the strongest erosion resistance when compared to other ratios between bioslurry and cementation solution. Subsequent experiments focused on separating the specimen into a "loose" part and a “surface crust,” with shear stresses …

Explainable Automated Inconsistency Detection In Biomedical And Health Literature, Prajwol Lamichhane

UNF Graduate Theses and Dissertations

Given the exponential growth of scientific information online, researchers often face the daunting task of detecting contradictory statements on crucial health topics. This work develops a comprehensive pipeline for automated contradiction detection that integrates an Information Retrieval (IR) system, machine learning classifiers, and Explainable AI (XAI). The Information Retrieval system is tailored for biomedical data and comprises a datastore, syntactic, and semantic components. Users can input queries, initiating a pipeline that identifies top documents through syntactic analysis and refines results via semantic examination for relevant research claims. Employing a diverse range of Large Language Models such as pre-trained Distil-BERT, BioBERT, …

Endophytic Fungi Associated With Galls And Host Plants Of Asphondylia Borrichiae (Diptera: Cecidomyiidae), Shannon Lynn Sharpe

UNF Graduate Theses and Dissertations

Asphondylia borrichiae, like other gall-forming gnats, are obligate symbionts to the endophytic fungi (EF) they deposit when laying eggs. Asphondylia spp. and EF are important biocontrol agents, yet details surrounding their diversity, life cycles, and host interactions remain poorly understood. Galls and leaf-stem tissues from two host plants were surveyed to identify and compare EF diversity between the host tissues and species of A. borrichiae. Identification of 114 isolates was carried out by sequencing the internal transcribed spacer (ITS 1/4 primers). Pairwise alignments in multiple genetic databases were bolstered by phylogenetic analyses using maximum parsimony and maximum likelihood …

Rose Nourish To Flourish Obesity Prevention Program Targeting Children At Risk For Food Insecurity In Jacksonville, Florida, Jamie Marchio

UNF Graduate Theses and Dissertations

The Nourish to Flourish study aimed to decrease the prevalence of obesity among food insecure children in Jacksonville, FL. Specifically, the program focused on nutrition education and access to healthy foods. The study consisted of a 4-week nutrition education series emphasizing the major determinants of healthy weight known as 5-2-1-0! This includes expanding knowledge of fruit and vegetable intake to achieve a target intake of five servings a day, screen time reduction to no more than two hours a day, achieving a physical activity goal of one hour a day outside of school hours, and limiting sugar sweetened beverage consumption …

Can A Phycosphere Associated Bacterium Be Used As A Biological Control For Floating Algal Mats?, Aubrey C. Patterson Holsworth

UNF Graduate Theses and Dissertations

Abstract

Freshwater harmful algal blooms (HABs) are a global water quality issue. Algae, especially cyanobacteria, are integrally associated with complex microbial assemblages, which cycle nutrients, provide microenvironments, breakdown potentially harmful molecules, etc. However, these same microbial consortia are competitors or even inhibitors to algae in these systems. One potential biological control is Rhodospirillum rubrum, a Gram- proteobacterium. To evaluate its capacity for HAB mitigation, experimental mesocosms were inoculated with a native assortment of algae and R. rubrum and grown for 2-months. Relative Fluorescence Units (RFU), Ash Free Dry Mass, and eukaryotic (23S rDNA) metabarcoding were conducted to estimate algal …

Implementation Of Algorithms For Sorting By Strip Swaps Using Alternating Cycle Graphs, Harsh S. Bhakharia

UNF Graduate Theses and Dissertations

Challenges in computational biology, such as genome rearrangement, and applications in optical character recognition, like zoning techniques, often require solving optimization problems. These involve reordering scrambled sequences of numbers into a sequential arrangement known as the identity permutation.

Rearranging any given permutation into the identity permutation with the minimum number of specific operations related to strips gives rise to various combinatorial optimization scenarios. Operations such as strip moves, transpositions, reversals, and block interchanges have been extensively researched.

This thesis builds on the foundational work of Roy et al., targeting the refinement of their cycle graph algorithm for sorting by strip …

Exploring The Welfare Of A Captive Population Of Cownose Rays (Rhinoptera Bonasus) Through Social And Resource Interactions, Kendal N. Rogers

UNF Graduate Theses and Dissertations

Zoos and aquariums utilize research-based management to ensure animals remain in a positive welfare standing. While vital, this research is taxa-biased towards mammals, with few studies observing other species that are frequently observed in captivity, such as elasmobranchs, or sharks and rays. Often maintained in exhibits that allow direct guest interaction, or touch pools, elasmobranchs have been shown to respond to these exposures through behavior changes in activity and social interactions. While this may indicate a potential for compromised welfare, the literature is conflicting and sparse, leaving uncertainty in the overall welfare condition of animals maintained in this popular exhibit …

Integrating Therapy Into Play: Stand-On Ride-On For A Child With Cerebral Palsy, Kira Flanagan

UNF Graduate Theses and Dissertations

This study focuses on the development of an adaptive ride-on toy specifically designed for a 2.5-year-old child with spastic diplegic cerebral palsy. As a feasibility study, the primary objective of this innovative device is to enhance motor function, foster autonomy, and improve the overall quality of life for the child. The Power Mobility Device (PMD) integrates actuation and steering modifications, and a harness mechanism tailored to meet the child's unique needs. Comprehensive assessments of the child's spatiotemporal gait characteristics were conducted before and after a three-month usage period. The results reveal significant improvements in the child's ability to control dynamic …

Florida Queer K12 Teachers Perceptions Of School Climate Under “Don’T Say Gay”, Katherine E. Foley

UNF Graduate Theses and Dissertations

Florida's newest legislation leaves many Florida queer teachers feeling marginalized and unprotected in the classroom (Block, 2022). The “Parental Rights in Education” Bill (FL HB 1557 and FL HB 1069), infamously renamed “Don’t Say Gay”, was created ostensibly to help protect parental rights and choices in education. The new law prohibits the discussion of gender identity and sexual orientation in kindergarten through twelfth grade classrooms. “Don’t Say Gay” provides vague guidelines for queer teachers on approaching topics like sexual orientation and gender identity. Despite the evidence that queer teachers who are open and honest about their identity often find themselves …

Evaluating The Use Of Baited Remote Underwater Video (Bruv) In Assessing Shark And Fish Populations In A Low Visibility Estuarine System, The Tolomato River, Fl, Usa, Sarah Worthington

UNF Graduate Theses and Dissertations

Recently, there has been increasing use of baited remote underwater video (BRUV) surveys for assessing shark and fish populations in marine ecosystems. This approach can be useful because it may provide a non-destructive, non-extractive, and cost-effective alternative to traditional fishing-based methods. However, to date, most studies using BRUVs have focused on their use in high-visibility ecosystems, such as tropical reefs. Furthermore, few studies have compared results from BRUV surveys with those obtained using traditional methods to evaluate the efficacy of this approach. Therefore, the goal of this study was to evaluate the effectiveness of using BRUV surveys to assess shark …

An Enhanced Evaluation Of Bioslurry For Use In Coastal Erosion Mitigation, Megan Elizabeth Dunton

UNF Graduate Theses and Dissertations

This paper discusses the treatment of Florida beach sand by surface percolating Bioslurry, a microbially-induced calcite precipitation (MICP) variant that was developed and refined in recent years. Researchers examined how variations in pore volume and surface area affected the cemented depth of soil treated via surface percolation. Results suggested that while the pore volume is an important factor that governs cementation in soil treated via surface percolation, it does not appear to be the only controlling factor and treatment volume per unit surface area is another important factor to consider when treating soil with Bioslurry using surface percolation. The implications …

Development And Characterization Of Ceramic Devices For Capillary Action In Microgravity For The Purpose Of Growing Vegetables In Space, Lucas S. Mougeot

UNF Graduate Theses and Dissertations

The future of humanity is often rendered in spectacular visions of a multiplanetary civilization with a booming space economy. Reaching this reality requires addressing one of the core needs of survival: food. Porous ceramics used for Passive Porous Tube Nutrient Delivery Systems (PPTNDS) offer a promising solution. In microgravity environments such as the International Space Station (ISS), fluid dynamics and liquid coagulation do not support conventional agricultural practices. Porous ceramic materials present a solution that enables nutrient solution diffusion via capillary action.

In this research, it was determined that extruding a wadding ceramic tube and sintering it at 1900 °F …

Learning From 50 Years Of Aboriginal Alcohol Programs: A Summary, Australian Indigenous Healthinfonet

Research outputs 2022 to 2026

This publication is a summary of key messages from the book, Learning from 50 years of Aboriginal alcohol programs: beating the grog in Australia by Peter d’Abbs and Nicole Hewlett.

The book provides a comprehensive overview of the many actions taken over the last 50 years to prevent and reduce alcohol harms among Aboriginal people with a focus on actions led by Aboriginal communities. This summary of the book was developed by the HealthInfoNet Alcohol and other Drugs Knowledge Centre in collaboration with the authors, and aims to provide an accessible synthesis of the information and lessons learned as …

Friendship, Connection And Loss: Everyday Digital Kinning And Digital Homing Among Chinese Transnational Grandparents In Perth, Australia, Catriona Stevens, Loretta Baldasar, Raelene Wilding

Research outputs 2022 to 2026

This chapter explores how practices of digital kinning and digital homing enacted through smartphones shape the experiences of Chinese older adults in Perth, Australia. Transnational grandparent carers must balance the risks of diminished social networks with the benefits of providing intergenerational care. Some form strong friendships in Perth, but many yearn for a leisured life with their friends in China. Findings illustrate the central role of digital migration practices in transnational families and friendships, not only sustaining distant support networks, but also building new communities in host settings. However, for some, the co-presence afforded through WeChat may intensify feelings of …

Managing Differences In The Family, Alina M. Baltazar

Faculty Publications

No abstract provided.

Critical Belief Analysis For Security Studies, Barnet D. Feingold, Michael W. Collier

EKUOPEN: Open Textbooks

Critical Belief Analysis for Security Studies is a companion volume to Security Analysis: A Critical-Thinking Approach. Critical Belief Analysis (CBA) is an emerging analytic technique for describing, explaining, and predicting decisions and actions of agents (actors, believers, decision-makers). CBA provides analysts a deeper understanding of agents’ points of view, assumptions, and the webs of belief supporting those points of view and assumptions. CBA also provides a framework for analysts to assess the quality of their final analytic efforts. While this volume is adapted to security studies and foreign policy analysis, the techniques included apply to any social science field analyzing …

Suspended Particulate Organic Matter Supports Mesopredatory Fish Across A Tropical Seascape, Molly Moustaka, Tahlia J. Bassett, Leah Beltran, Michael V.W. Cuttler, Richard D. Evans, Daniel Gorman, Camille M. Grimaldi, Renee K. Gruber, Glenn A. Hyndes, Gary A. Kendrick, Natalie Travaglione, Shaun K. Wilson

Research outputs 2022 to 2026

Understanding the sources of primary production supporting marine food webs is important for conserving critical habitats. However, the importance of allochthonous versus autochthonous production is often unclear. We identify the sources of primary production supporting three species of predatory fish (Lutjanus carponotatus, Lutjanus fulviflamma, and Lethrinus punctulatus) in the Dampier Archipelago, Western Australia. The proportions of mangrove, seagrass, macroalgae, and suspended particulate organic matter (SPOM) assimilated by fish of different life-history stages and from different habitats were determined using stable isotopes and Bayesian mixing models. While contributions of sources varied among species and habitats, we found that SPOM was an …

Towards An Indigenous-Informed Multispecies Collaboratory, Sandra Wooltorton, Peta J. White

Research outputs 2022 to 2026

How does Australia's latest Indigenous defeat relate to Environmental Education? The answer is direct complicity. This paper begins with the premise that the failure of Australia's 2023 referendum on "The Voice to Parliament"is directly connected with education. The chapter builds on the proposition that local and Indigenous public knowledge could - and should - be the heart of environmental education. We apply a post-qualitative practice that is underpinned by innovative feminisms and the post-qualitative methods within a Multispecies Collaboratory, an experimental way of transforming our learning by attending to the responsive, relational world of all beings. We use this practice …

Variations In The Germinal Center Response Revealed By Genetically Diverse Mouse Strains, Anne Marie Aubin, Daria Vdovenko, Roxanne Collin, Lois Balmer, Lise Coderre, Grant Morahan, Félix Lombard-Vadnais, Sylvie Lesage

Research outputs 2022 to 2026

The humoral response is complex and involves multiple cellular populations and signaling pathways. Bacterial and viral infections, as well as immunization regimens, can trigger this type of response, promoting the formation of microanatomical cellular structures called germinal centers (GCs). GCs formed in secondary lymphoid organs support the differentiation of high-affinity plasma cells and memory B cells. There is growing evidence that the quality of the humoral response is influenced by genetic variants. Using 12 genetically divergent mouse strains, we assessed the impact of genetics on GC cellular traits. At steady state, in the spleen, lymph nodes and Peyer's patches, we …

Rapid Analysis Of N-Nitrosamines In Urine Using Ultra High-Pressure Liquid Chromatography-Mass Spectrometry, S. Shinde, K. D. Croft, J. M. Hodgson, C. P. Bondonno

Research outputs 2022 to 2026

N-Nitrosamines, carcinogenic compounds present in dietary and environmental sources and formed endogenously, are believed to be linked with the presence of nitrate and nitrite, both within dietary sources and after intake. To fully evaluate this potential threat to human health, an accurate analytical method to measure N-nitrosamines in biological matrices is necessary. We report a simple, fast, selective mass spectrometry method to detect N-nitrosamines in human urine. Analysis of seven N-nitrosamines, N-nitrosodimethylamine (NDMA), N-nitrosomethylethylamine (NMEA), N-nitrosodiethylamine (NDEA), N-nitrosopiperdine (NPIP), N-nitrosopyrrolidine (NPYR), N-nitrosodi-N-propylamine (NDPA) and N-nitrosodi-N-butylamine (NDBA) in urine was quantitated using Ultra High-Pressure Liquid Chromatography-tandem Mass spectrometry (UHPLC-MS/MS). A Sorbent …

A 16-Week Progressive Exercise Training Intervention In Treatment-Naïve Chronic Lymphocytic Leukaemia: A Randomised-Controlled Pilot Study, Frankie F. Brown, Rebecca Oliver, Rachel Eddy, Adam J. Causer, Annabelle Emery, Harrison D. Collier-Bain, David Dutton, Josephine Crowe, Daniel Augustine, John Graby, Daniel Rees, Daniela Rothschild-Rodriguez, Oliver J. Peacock, Sally Moore, James Murray, James E. Turner, John P. Campbell

Research outputs 2022 to 2026

Background: Chronic lymphocytic leukaemia (CLL) typically presents with asymptomatic, early-stage disease that is monitored until disease progression (‘treatment-naïve’ CLL). The objective of this pilot study was to assess the feasibility and preliminary safety of an exercise program in treatment-naïve CLL. We also sought to preliminarily assess the impact of the exercise program on disease activity, as it has been proposed that exercise training may reduce disease outgrowth in treatment-naïve CLL. Methods: A total of 40 treatment-naïve CLL patients were recruited into this randomised-controlled pilot study, and after screening, n = 28 were randomised into a 16-week, home-based, partially supervised, personalised, …