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Nebraska Test #2288: Case Ih Steiger 475 Rowtrac, Nebraska Tractor Test Lab Jan 2024

Nebraska Test #2288: Case Ih Steiger 475 Rowtrac, Nebraska Tractor Test Lab

Nebraska Tractor Tests

ABOUT THE TEST REPORT AND USE OF THE DATA The test data contained in this report are a tabulation of the results of a series of tests. Due to the restricted format of these pages, only a limited amount of data and not all of the tractor specifications are included. The full OECD report contains usually about 30 pages of data and specifications. The test data were obtained for each tractor under similar conditions and therefore, provide a means of comparison of performance based on a limited set of reported data. EXPLANATION OF THE TEST PROCEDURES Purpose The purpose of …


Tractor Test 2281: John Deere 9rt 540 Diesel, Nebraska Tractor Test Lab Jan 2024

Tractor Test 2281: John Deere 9rt 540 Diesel, Nebraska Tractor Test Lab

Nebraska Tractor Tests

ABOUT THE TEST REPORT AND USE OF THE DATA The test data contained in this report are a tabulation of the results of a series of tests. Due to the restricted format of these pages, only a limited amount of data and not all of the tractor specifications are included. The full OECD report contains usually about 30 pages of data and specifications. The test data were obtained for each tractor under similar conditions and therefore, provide a means of comparison of performance based on a limited set of reported data. EXPLANATION OF THE TEST PROCEDURES Purpose The purpose of …


Exploring Women`S Perceptions Of Climate Change Impact On Agriculture, Health & Food Security In Upper Hunza, Gilgit Baltistan, Ambreen, Elina Nizar Ali, Taiba Yar Baig, Fozia Parveen Jan 2024

Exploring Women`S Perceptions Of Climate Change Impact On Agriculture, Health & Food Security In Upper Hunza, Gilgit Baltistan, Ambreen, Elina Nizar Ali, Taiba Yar Baig, Fozia Parveen

Institute for Educational Development, Karachi

The Hindu Kush Himalayan (HKH) region, renowned for its towering mountains and major river basins, sustains nearly 1.4 billion people worldwide and is pivotal to global food production. Glacial melt from the HKH region nourishes agriculture, livestock, and horticulture, supporting the livelihoods of mountain communities. However, climate change is accelerating the melting of glaciers, and shifts in wet seasons significantly impact food security in these communities. Sectors such as water resources, agricultural land, and human health, particularly women’s health, are significantly affected by these changes. For centuries, agriculture has been the backbone of Gilgit-Baltistan’s economy, with over 70% of livelihoods …


A Quest For Gender Equity: Exploring The Existing Positioning Of Children And Teachers In An Early Childhood Classroom In Karachi Pakistan, Nasima Shakeel, Dilshad Ashraf Jan 2024

A Quest For Gender Equity: Exploring The Existing Positioning Of Children And Teachers In An Early Childhood Classroom In Karachi Pakistan, Nasima Shakeel, Dilshad Ashraf

Institute for Educational Development, Karachi

Within the framework of Sustainable Development Goals, children are recognized as active change agents in the global development agenda (The Lancet, 2022). Research has established that engaging young children has the potential to promote gender equity and reduce other inequities in society (UN Women 2020). Despite this, limited research has been conducted on gender equity issues in education and early childhood education particularly in Pakistan (Pardhan & Pelletier, 2016). In response to this gap, collaborative action research was conducted to explore the possibilities of creating a gender-equitable learning environment in an early childhood classroom in Karachi Pakistan. This paper presents …


Naomi Kinney Honors Portfolio, Naomi Kinney Jan 2024

Naomi Kinney Honors Portfolio, Naomi Kinney

Honors Capstone Portfolios

Naomi Kinney's Honors Portfolio captured in August 2024.


Carly Abelson Honors Portfolio, Carly Abelson Jan 2024

Carly Abelson Honors Portfolio, Carly Abelson

Honors Capstone Portfolios

Carly Abelson's Honors Portfolio captured in December 2024.


Characterization Of Nuclear Microsatellites In Marchantia Polymorpha (Liverwort) With Additional Trans-Specific Analyses, Nicole Rodriguez Ortiz, Niharika Sharma, Tian Xiong Zheng, James J. Campanella Jan 2024

Characterization Of Nuclear Microsatellites In Marchantia Polymorpha (Liverwort) With Additional Trans-Specific Analyses, Nicole Rodriguez Ortiz, Niharika Sharma, Tian Xiong Zheng, James J. Campanella

Department of Biology Faculty Scholarship and Creative Works

Microsatellites are present in mitochondria, chloroplast, and nuclear DNA, but nuclear microsatellites are more useful genetic tools than those in plastids or mitochondria. Plastid and mitochondrial microsatellites have been identified in the model plant Marchantia polymorpha (liverwort), but no laboratory has published information on nuclear microsatellite loci. The aim of this study was to detect novel nuclear markers in the most commonly employed liverwort species, design PCR primers that would allow amplification, and characterize the subsequently generated loci. We detected 18 polymorphic nuclear loci in M. polymorpha, amplifiable by PCR across all chromosomes. Additionally, trans-specific amplification of the eighteen loci …


Perceived Linguistic And Non-Linguistic Barriers Facing Saudi Female Partners Learning English, Samah Mohamad Dulli Jan 2024

Perceived Linguistic And Non-Linguistic Barriers Facing Saudi Female Partners Learning English, Samah Mohamad Dulli

Electronic Theses and Dissertations

The partners of the Saudi students who come to the United States on scholarships to earn a degree (Bachelor's, Master's, or Ph.D.) are eligible for full scholarships as well. However, most of them fail to proceed in earning a degree or fulfill the language proficiency requirement needed to join an academic degree program. This study surveys between 10 to 20 adult female partners of Saudi university students to elicit their opinions on how much English they think they will learn during their partner scholarship time in academic and non-academic settings. This study investigates the female partners of the Saudi degree …


Punishing Poverty: An Examination Of Financial Disadvantage And Out-Of-School Suspension, Joshua Rosenbaum Jan 2024

Punishing Poverty: An Examination Of Financial Disadvantage And Out-Of-School Suspension, Joshua Rosenbaum

Electronic Theses and Dissertations

School suspension is often a response by officials to address deviant behavior within the school setting. While studies have examined various causes and consequences of school suspension, there is a dearth of literature focusing on the effects of financial disadvantage on school discipline (i.e., school suspension). Given that out-of-school suspension is a reliable predictor of juvenile justice involvement later in life, it serves as an important outcome variable in preventative criminology. Using data from the National Longitudinal Study of Adolescent to Adult Health (Add Health), the purpose of the current study is to analyze the relationship between financial disadvantage and …


Ftir Microspectroscopy For The Investigation Of Microplastic Pollution, Automotive Paint Layers, And Prehistoric Rock Paint With An Emphasis On Analytical Method Development, Kendall Wontor Jan 2024

Ftir Microspectroscopy For The Investigation Of Microplastic Pollution, Automotive Paint Layers, And Prehistoric Rock Paint With An Emphasis On Analytical Method Development, Kendall Wontor

Electronic Theses and Dissertations

The research detailed in this dissertation centers on the development of new analytical methods using chemical imaging microspectroscopy, primarily μ[micro]-FTIR, for applications in environmental, forensic, and archeological chemistry. The state-of-the-art instrument (LUMOS II) used in this research combines optical microscopy with IR spectroscopy, allowing sample visualization during FTIR imaging and micro-analysis.

Chapter 1 serves as an introduction and provides background detail on the instrumentation and technique. Chapter 2 describes the use of fluorescent microscopy to quantify microplastics (MPs) in oysters from the Mississippi Sound. High concentrations of putative MPs were found, with sites inside bays near population centers displaying higher …


Breaking Old: Trends And Adverse Events Of Prescription Stimulant Use In Older Adults, Saumil Jadhav Jan 2024

Breaking Old: Trends And Adverse Events Of Prescription Stimulant Use In Older Adults, Saumil Jadhav

Electronic Theses and Dissertations

This dissertation examined prescription stimulant medication (PSM) use among older adults in the United States from 2012-2020 by (1) assessing annual prevalence and describing clinical characteristics and treatment patterns among new PSM users, (2) evaluating the association between PSM initiation and risk of adverse events such as stroke, myocardial infarction, and all-cause mortality, and (3) analyzing trends in the concomitant use of PSMs and antidepressants. The study utilized 5% national sample of Medicare administrative claims from 2012-2020.

First, the study identified an increase in the annual prevalence of PSM use from 0.44% in 2012 to 0.63% in 2020 among older …


Formulation And Characterization Of Quetiapine Fumarate Floating Bioadhesive Tablets, Ajay Reddy Kolipaka Jan 2024

Formulation And Characterization Of Quetiapine Fumarate Floating Bioadhesive Tablets, Ajay Reddy Kolipaka

Electronic Theses and Dissertations

Quetiapine fumarate (QF) is an antipsychotic drug, and it has a plasma half-life of 6 hours. The chemical structure of QF has nitrogen groups making it highly soluble in acidic pH and slightly soluble in alkaline pH. This study aimed to develop the QF gastroretentive tablets by TSMG technology comprising floating and mucoadhesive approach by using different viscosity grades of HPMC to improve absorption by prolonging its gastric residence time. The floating tablets were prepared using the Twin screw melt granulation (TSMG) technology, and the impact of polymer concentration and viscosity grades of HPMC on the floating ability and drug …


Land For Whom? Diversity, Land Trusts, And Farmers And Gardeners From Marginalized Backgrounds In New England, U.S., Julie C. Keller, Blake Harrison, Corey Lang Jan 2024

Land For Whom? Diversity, Land Trusts, And Farmers And Gardeners From Marginalized Backgrounds In New England, U.S., Julie C. Keller, Blake Harrison, Corey Lang

Environmental and Natural Resource Economics Faculty Publications

Land trusts in the U.S. play an important role in preventing the loss of farmland to development. Farmers seeking affordable land may benefit from these conservation efforts. Yet the distribution of these benefits across farmer groups is unclear. This study asks: To what extent are land trusts meeting the needs of farmers and gardeners from marginalized backgrounds? Interviews with land trust staff and focus groups with marginalized farmers and gardeners in New England, USA reveal discrepancies in how diversity and land access are discussed. Access to adequate, affordable land was a serious concern among farmers and gardeners, yet few land …


Sacred Discrimination: Latvian Lutheran Clergy Against Ordination Of Women, Ģirts Rozners Jan 2024

Sacred Discrimination: Latvian Lutheran Clergy Against Ordination Of Women, Ģirts Rozners

Occasional Papers on Religion in Eastern Europe

This paper examines the resistance of contemporary Latvian Lutheran clergy to the ordination of women, rooted in historical factors and theological conservatism. It discusses the impact of Latvia's tumultuous history on theological discourse, revealing how practical concerns and traditionalism influenced the decision against women's ordination. Through clergy interviews, it uncovers a range of arguments used to justify this stance, exposing elements of sexism and neoconservatism. The clergy's prioritization of community identity over societal progress is evident, as they align with hierarchical leadership and resist external influences. This study highlights the disconnect between theological tradition and modern ethics, illustrating how theology …


"We Don’T Need No Ordination": Analysis Of The Discourse On Women’S Ordination In Latvian Online Media From 1999 To 2022, Emīls Zavelis, Sofija Anna Kozlova Jan 2024

"We Don’T Need No Ordination": Analysis Of The Discourse On Women’S Ordination In Latvian Online Media From 1999 To 2022, Emīls Zavelis, Sofija Anna Kozlova

Occasional Papers on Religion in Eastern Europe

This paper analyzes the discourse on women's ordination in the Evangelical Lutheran Church of Latvia (ELCL) as represented in Latvian online media from 1999 to 2022. The study combines qualitative and quantitative methodologies to examine the argumentative strategies employed by different discourse producers. The findings reveal significant differences in the use of gender equality concepts and other argumentative patterns between those in favor and those against women's ordination. These differences can be attributed to the distinct sociocultural contexts and epistemic grounds of the ELCL and the Evangelical Lutheran Church of Latvia Worldwide (ELCLW). The paper highlights the need for a …


The Differential Proteomic Response To Ischemic Stroke In Appalachian Subjects Treated With Mechanical Thrombectomy, Christopher J. Mclouth, Benton Maglinger, Jacqueline A. Frank, Kristin J. Hazelwood, Jordan P. Harp, Will Cranford, Shivani Pahwa, David Dornbos Iii, Amanda L. Trout, Ann M. Stowe, Justin F. Fraser, Keith R. Pennypacker Jan 2024

The Differential Proteomic Response To Ischemic Stroke In Appalachian Subjects Treated With Mechanical Thrombectomy, Christopher J. Mclouth, Benton Maglinger, Jacqueline A. Frank, Kristin J. Hazelwood, Jordan P. Harp, Will Cranford, Shivani Pahwa, David Dornbos Iii, Amanda L. Trout, Ann M. Stowe, Justin F. Fraser, Keith R. Pennypacker

Neurology Faculty Publications

Introduction The Appalachia region of North America is known to have significant health disparities, specifically, worse risk factors and outcomes for stroke. Appalachians are more likely to have comorbidities related to stroke, such as diabetes, obesity, and tobacco use, and are often less likely to have stroke interventions, such as mechanical thrombectomy (MT), for emergent large vessel occlusion (ELVO). As our Comprehensive Stroke Center directly serves stroke subjects from both Appalachian and non-Appalachian areas, inflammatory proteomic biomarkers were identified associated with stroke outcomes specific to subjects residing in Appalachia. Methods There were 81 subjects that met inclusion criteria for this …


Screening Incarcerated Women For Opioid Use Disorder, Michele Staton, Martha Tillson, Mary M. Levi, Matthew Webster, Carrie B. Oser, Carl Leukefeld Jan 2024

Screening Incarcerated Women For Opioid Use Disorder, Michele Staton, Martha Tillson, Mary M. Levi, Matthew Webster, Carrie B. Oser, Carl Leukefeld

Behavioral Science Faculty Publications

The overall aim of the present study is to examine the utility of the DSM OUD Checklist and the NM-ASSIST screening tools to identify symptoms consistent with OUD among incarcerated women in county jails. This study contributes to the existing literature because research on screening and assessment approaches for incarcerated women has been limited. The focus of the current study is to describe the screening procedures and study recruitment for a larger parent study focused on increasing treatment linkages. Study findings indicate a positive correlation between indicators of OUD using the two screening tools, as well as a high degree …


Assessing Understory Vegetation Response And Fuel Load Levels Following Silvicultural Treatments In Headwaters Forest Reserve, Humboldt County, California, Kyle James Railton, Madison Tatar Jan 2024

Assessing Understory Vegetation Response And Fuel Load Levels Following Silvicultural Treatments In Headwaters Forest Reserve, Humboldt County, California, Kyle James Railton, Madison Tatar

Undergraduate Scholarship

Old growth forests are important for ecological resilience, especially in temperate rainforest ecosystems. There are presently 3,000 acres of old growth forest within the 7,472 acres of the Headwaters Forest Reserve (HFR) in Humboldt County, California. Remaining acres were subjected to unsustainable logging practices, such as clearcutting, that resulted in a severely overstocked second-growth forest. The Bureau of Land Management (BLM) created a management plan for the reserve to restore previously harvested areas to their historic condition. The goal for the BLM is to accelerate growth rates of residual forests, to align them on a trajectory of achieving old-growth forest …


Examining Coastal Redwood Forest Understory Vegetation As Socio-Ecological Indicators Of Health And Resilience In Headwaters Forest Reserve, California, Rhiannon Lopez Jan 2024

Examining Coastal Redwood Forest Understory Vegetation As Socio-Ecological Indicators Of Health And Resilience In Headwaters Forest Reserve, California, Rhiannon Lopez

Undergraduate Scholarship

Coastal redwood old-growth forests are widely considered as some of the most ecologically significant landscapes, rich in both cultural and environmental history. These forests have provided essential habitat for threatened species and support a complex web of life for flora, fauna, and Indigenous people since time immemorial. However, the forced removal of Indigenous peoples, loss of Indigenous stewardship and the prioritization of Western management objectives–such as intensive logging– have resulted in habitat degradation and fragmentation, severely disrupting ecological cycles. Approximately 95% of Coastal redwood old-growth forests have been lost to extractive industries, with the remaining 5% mostly held in conservation …


Abnormalities In Pharyngeal Arch-Derived Structures In Satb2-Associated Syndrome, Yuri A. Zarate, Katherine Bosanko, Nada Derar, Jennifer L. Fish Jan 2024

Abnormalities In Pharyngeal Arch-Derived Structures In Satb2-Associated Syndrome, Yuri A. Zarate, Katherine Bosanko, Nada Derar, Jennifer L. Fish

Pediatrics Faculty Publications

SATB2-associated syndrome (SAS, glass syndrome, OMIM#612313) is a neurodevelopmental autosomal dominant disorder with frequent craniofacial abnormalities including palatal and dental anomalies. To assess the role of Satb2 in craniofacial development, we analyzed mutant mice at different stages of development. Here, we show that Satb2 is broadly expressed in early embryonic mouse development including the mesenchyme of the second and third arches. Satb2-/- mutant mice exhibit microglossia, a shortened lower jaw, smaller trigeminal ganglia, and larger thyroids. We correlate these findings with the detailed clinical phenotype of four individuals with SAS and remarkable craniofacial phenotypes with one requiring mandibular distraction in …


The Impact Of The National Breeding Index For Dairy Cows On The Beef Merit Of Their Progeny, Shauna Mulhall Jan 2024

The Impact Of The National Breeding Index For Dairy Cows On The Beef Merit Of Their Progeny, Shauna Mulhall

Theses

The expansion of the dairy herd, along with enhanced fertility in dairy cows, and increased adoption of sexed semen technologies, has resulted in a growing number of beef cattle originating from the dairy sector. Breeding programs in dairy cows have been primarily focused on improving fertility and milk production, with limited attention given to progeny intended for beef production. To establish a sustainable dairy and beef sector, enhancing the performance and efficiency of beef calves from dairy herds is crucial. This thesis aims to assess how current breeding strategies in Irish dairy farming impact downstream beef merit. The thesis will …


Improving The Most Gg Model To Predict Environmental Impact And Improve On Farm Grass Growth Prediction, Laeticia Bonnard Jan 2024

Improving The Most Gg Model To Predict Environmental Impact And Improve On Farm Grass Growth Prediction, Laeticia Bonnard

Theses

The increasing need for highly productive and eco-friendly agriculture is driving demand for decision-support tools to assist agricultural operators in managing systems more precisely and sustainably. Although grasslands provide a cost-effective option for ruminant production and can offer many environmental benefits (biodiversity, carbon storage etc.), grasslands management is challenging due to the inherent climate and soil sensitivities and their potential negative impact on water quality. In Ireland, the Moorepark Saint Gilles Grass Growth (MoSt GG) model was developed for the dual purposes of research-based simulations and on-farm grass growth prediction for farmers. The objective of this thesis was to improve …


Optima: Optimal Protein For Optimal Ageing, Eunice Ti-Oluwani Olaniyan Jan 2024

Optima: Optimal Protein For Optimal Ageing, Eunice Ti-Oluwani Olaniyan

Theses

The older adult population is increasing globally, and this is associated with an increased prevalence of chronic diseases. This, together with increased consumer concern regarding the overconsumption of animal proteins, has led to a need to consider alternative dietary strategies for older adults. This study developed novel plant protein blends using combinations of pumpkin, pea ad rice protein isolates, and investigated their amino acid profiles, protein content and bioactive properties to identify optimal blends for functional food formulation. A major objective of this study was to formulate protein-rich products. It was hypothesised that blending multiple sources of plant proteins in …


Envisioning Munster Technological University As A Trauma-Sensitive University, Marie Singleton Jan 2024

Envisioning Munster Technological University As A Trauma-Sensitive University, Marie Singleton

Theses

Trauma-informed care works on the assumption that individuals utilizing relevant services have experienced trauma (Palmer, 2020). The trauma-informed approach focuses on providing empathy and compassion to create healing relationships and ensure accessibility for all service users. This approach is aimed at understanding, identifying, and addressing trauma in organisations by making changes to practices, policies, and procedures (Champine et al., 2022). Substance Abuse and Mental Health Services Administration (SAMHSA) (2014:10) states that ‘trauma results from an event, serie s of events, or set of circumstances that is experienced by an individual as physically or emotionally harmful or threatening and that has …


Improving Long-Term Sustainable Profit For Beef Producers And Processors Through Genetic Selection, Stephen Connolly Jan 2024

Improving Long-Term Sustainable Profit For Beef Producers And Processors Through Genetic Selection, Stephen Connolly

Theses

The thesis served to quantify the phenotypic performance of animals differing in genetic merit based on the Irish Terminal Breeding Index. The data in the thesis included performance metrics of interest to both the primary producer and the meat processing industry. It is anticipated that the findings of the thesis will instil a level of confidence among primary producers and meat processors whereby the current terminal breeding objective is generating beef cattle with superior carcass performance, and improved farm margins along with a more desirable animal for meat processors. The impact of genetic differences among animals for performance traits on …


Relationships Between Cliff Swallows (Petrochelidon Pyrrhonota) And Feather Coloration, Morphological Characteristics, And Colony Size, Sonja M. Brandt Jan 2024

Relationships Between Cliff Swallows (Petrochelidon Pyrrhonota) And Feather Coloration, Morphological Characteristics, And Colony Size, Sonja M. Brandt

Master's Theses or Doctor of Nursing Practice

Environmental stressors such as drought and roadways can strongly affect breeding birds. Cliff Swallows (Petrochelidon Pyrrhonota) are aerial insectivores that experience mortality from car collisions, because they often nest and hunt under bridges and road culverts. Individual traits that may convey social information, such as body condition, or feather coloration, might be associated with better coping around cars and other environmental stressors. Few studies have investigated relationships between coloration and other environmental stressors, such as water loss due to drought near the colony and foraging areas. Colony size can be heavily affected by various environmental factors such as water level …


The Physiological And Behavioral Effects Of A Centrally Administered Oxytocin Receptor Antagonist On Maternal Buffering In Infant Guinea Pigs, Kendra A. Carter Jan 2024

The Physiological And Behavioral Effects Of A Centrally Administered Oxytocin Receptor Antagonist On Maternal Buffering In Infant Guinea Pigs, Kendra A. Carter

Browse all Theses and Dissertations

This study investigates the role of endogenous oxytocin (OT) in maternal buffering of infant stress responses in guinea pigs. Infants received intracerebroventricular infusion of an oxytocin receptor antagonist (OTA) or artificial cerebrospinal fluid (aCSF) immediately before being placed with the mother in a novel environment for 60 minutes. Blood cortisol levels and behavioral responses, including vocalizations and crouching, were measured throughout the testing period. Results indicate only a significant effect of time, with cortisol levels increasing with time spent in the novel environment. Otherwise, no significant differences were observed either physiologically or behaviorally between aCSF and OTA conditions. This study …


Retracing Their Tracks: The Rediscovery And Memorialization Efforts Of The National Orphan Train Project, Joanna Lockwood Jan 2024

Retracing Their Tracks: The Rediscovery And Memorialization Efforts Of The National Orphan Train Project, Joanna Lockwood

Master's Theses or Doctor of Nursing Practice

Between 1854 and 1929, the placement system that became known as the orphan trains in modern scholarship, removed neglected and orphaned children from the dangerous streets of New York City and attempted to place them into homes across America that would benefit their futures. While this charity-run program was well-intentioned, the estimated two hundred fifty thousand children of the orphan trains experienced a mix of encounters upon their placements. Although many homes provided a caring environment for these children, others did not. After years of struggle during their early childhood, numerous children had not only lost their parents and were …


Bacterial Metallophores As Potential Agents In Heavy Metal Bioremediation, Mohammed Ahmed Jan 2024

Bacterial Metallophores As Potential Agents In Heavy Metal Bioremediation, Mohammed Ahmed

Electronic Theses and Dissertations

Heavy metal pollution is a significant threat to human health and the environment. Bacteria have evolved diverse mechanisms to adapt to these pollutants, including the secretion of small molecules called metallophores. Bacterial metallophores are metabolites excreted in response to toxic metal stress to protect the bacterial cell. These small molecules have shown versatility in potential applications for the remediation of heavy metals. This research focuses on constructing a library of bacterial metallophores, which will facilitate the assessment of their metal-binding properties when exposed to different metals. The study has two main areas of investigation: Firstly, it involves isolating and characterizing …


Astrocytic Mitochondrial Transfer To Brain Endothelial Cells And Pericytes In Vivo Increases With Aging, Gopal V. Velmurugan, Hemendra J. Vekaria, Samir P. Patel, Patrick G. Sullivan, W. Brad Hubbard Jan 2024

Astrocytic Mitochondrial Transfer To Brain Endothelial Cells And Pericytes In Vivo Increases With Aging, Gopal V. Velmurugan, Hemendra J. Vekaria, Samir P. Patel, Patrick G. Sullivan, W. Brad Hubbard

Neurology Faculty Publications

Intercellular mitochondrial transfer (IMT) is an intriguing biological phenomenon where mitochondria are transferred between different cells and notably, cell types. IMT is physiological, occurring in normal conditions, but also is utilized to deliver healthy mitochondria to cells in distress. Transferred mitochondria can be integrated to improve cellular metabolism, and mitochondrial function. Research on the mitochondrial transfer axis between astrocytes and brain capillaries in vivo is limited by the cellular heterogeneity of the neurovascular unit. To this end, we developed an inducible mouse model that expresses mitochondrial Dendra2 only in astrocytes and then isolated brain capillaries to remove all intact astrocytes. …