Digital Commons Network^™

Role Of Vitamins A, C, D, E In Cancer Prevention And Therapy: Therapeutic Potentials And Mechanisms Of Action, Wamidh H. Talib, Dima Abdulraheem Ahmed Jum’Ah, Zeena Shamil Attallah, Mohanned Sami Jallad, Lina T. Al Kury, Rawan Wamidh Hadi, Asma Ismail Mahmod

All Works

Cancer, a leading global cause of mortality, arises from intricate interactions between genetic and environmental factors, fueling uncontrolled cell growth. Amidst existing treatment limitations, vitamins have emerged as promising candidates for cancer prevention and treatment. This review focuses on Vitamins A, C, E, and D because of their protective activity against various types of cancer. They are essential as human metabolic coenzymes. Through a critical exploration of preclinical and clinical studies via PubMed and Google Scholar, the impact of these vitamins on cancer therapy was analyzed, unraveling their complicated mechanisms of action. Interestingly, vitamins impact immune function, antioxidant defense, inflammation, …

Multiplex Immunofluorescence Captures Progressive Immune Exhaustion With Advancing Penile Squamous Cell Cancer Stage, Filip Ionescu, Jonathan Nguyen, Carlos Moran Segura, Mahati Paravathaneni, G Daniel Grass, Peter Johnstone, Niki M Zacharias, Curtis A Pettaway, Xin Lu, Youngchul Kim, Junmin Whiting, Jasreman Dhillon, Steven A Eschrich, Juskaran Chadha, Keerthi Gullapalli, Gabriel Roman Souza, Hiroko Miyagi, Brandon J Manley, Philippe E Spiess, Jad Chahoud

Faculty, Staff and Student Publications

Penile squamous cell carcinoma (PSCC) is a rare and deadly malignancy. Therapeutic advances have been stifled by a poor understanding of disease biology. Specifically, the immune microenvironment is an underexplored component in PSCC and the activity of immune checkpoint inhibitors observed in a subset of patients suggests immune escape may play an important role in tumorigenesis. Herein, we explored for the first time the immune microenvironment of 57 men with PSCC and how it varies with the presence of human papillomavirus (HPV) infection and across tumor stages using multiplex immunofluorescence of key immune cell markers. We observed an increase in …

Applications Of Bioinformatics For Exploring The Genomic Landscape Of Cancer And Characterizing Treatment Response For Precision Oncology, Sharon Freshour

Arts & Sciences Graduate Student Theses and Dissertations

While the integration of next-generation sequencing and bioinformatics into cancer research has been immeasurably useful for advancing the field, there are still many gaps in understanding the genomic landscape of cancer due to the complexity of cancer development and evolution. Likewise, there are still many challenges for identifying and applying effective therapeutics for cancer treatment. Thus, there remains a need to continue characterizing the genomic landscape of cancer, to explore the evolution of cancer, and to understand how tumors respond to treatment. The projects described in this dissertation cover several applications of next-generation sequencing, bioinformatic analysis, and database resources for …

Aging Fly Cell Atlas Identifies Exhaustive Aging Features At Cellular Resolution, Kenneth A Wilson, Sudipta Bar, Eric B Dammer, Enrique M Carrera, Brian A Hodge, Tyler A U Hilsabeck, Joanna Bons, George W Brownridge, Jennifer N Beck, Jacob Rose, Melia Granath-Panelo, Christopher S Nelson, Grace Qi, Akos A Gerencser, Jianfeng Lan, Alexandra Afenjar, Geetanjali Chawla, Rachel B Brem, Philippe M Campeau, Hugo J Bellen, Birgit Schilling, Nicholas T Seyfried, Lisa M Ellerby, Pankaj Kapahi

Faculty, Staff and Students Publications

Dietary restriction (DR) delays aging, but the mechanism remains unclear. We identified polymorphisms in mtd, the fly homolog of OXR1, which influenced lifespan and mtd expression in response to DR. Knockdown in adulthood inhibited DR-mediated lifespan extension in female flies. We found that mtd/OXR1 expression declines with age and it interacts with the retromer, which regulates trafficking of proteins and lipids. Loss of mtd/OXR1 destabilized the retromer, causing improper protein trafficking and endolysosomal defects. Overexpression of retromer genes or pharmacological restabilization with R55 rescued lifespan and neurodegeneration in mtd-deficient flies and endolysosomal defects in fibroblasts from patients with lethal loss-of-function …

Exploiting The Carboxylate-Binding Pocket Of Β-Lactamase Enzymes Using A Focused Dna-Encoded Chemical Library, Suhyeorn Park, Jiayi Fan, Srinivas Chamakuri, Murugesan Palaniappan, Kiran Sharma, Xuan Qin, Jian Wang, Zhi Tan, Allison Judge, Liya Hu, Banumathi Sankaran, Feng Li, B V Venkataram Prasad, Martin M Matzuk, Timothy Palzkill

Faculty, Staff and Students Publications

β-Lactamase enzymes hydrolyze and thereby provide bacterial resistance to the important β-lactam class of antibiotics. The OXA-48 and NDM-1 β-lactamases cause resistance to the last-resort β-lactams, carbapenems, leading to a serious public health threat. Here, we utilized DNA-encoded chemical library (DECL) technology to discover novel β-lactamase inhibitors. We exploited the β-lactamase enzyme-substrate binding interactions and created a DECL targeting the carboxylate-binding pocket present in all β-lactamases. A library of 10

Pcl And Dmso2 Composites As Biomaterials For Additive Manufacturing, Jae-Won Jang

Dissertations and Theses

Tissue engineering provides a viable solution to address the shortage of organ donors for allograft transplants. Its primary objective is the replacement or regeneration of damaged tissues which is developed by combining living cells, a scaffold, and a bioreactor. Among these components, the scaffold serves as an artificial extracellular matrix (ECM), which plays a pivotal role in supporting cell adhesion, proliferation, differentiation, and overall tissue development. Additive manufacturing (AM), commonly referred to as three-dimensional (3D) printing, emerges as a promising method for scaffold fabrication due to its capability to create complex and repeatable geometries.

Polycaprolactone (PCL) has been one of …

Reactivation Of The G1 Enhancer Landscape Underlies Core Circuitry Addiction To Swi/Snf, Katerina Cermakova, Ling Tao, Milan Dejmek, Michal Sala, Matthew D Montierth, Yuen San Chan, Ivanshi Patel, Courtney Chambers, Mario Loeza Cabrera, Dane Hoffman, Ronald J Parchem, Wenyi Wang, Radim Nencka, Eveline Barbieri, H Courtney Hodges

Faculty, Staff and Student Publications

Several cancer core regulatory circuitries (CRCs) depend on the sustained generation of DNA accessibility by SWI/SNF chromatin remodelers. However, the window when SWI/SNF is acutely essential in these settings has not been identified. Here we used neuroblastoma (NB) cells to model and dissect the relationship between cell-cycle progression and SWI/SNF ATPase activity. We find that SWI/SNF inactivation impairs coordinated occupancy of non-pioneer CRC members at enhancers within 1 hour, rapidly breaking their autoregulation. By precisely timing inhibitor treatment following synchronization, we show that SWI/SNF is dispensable for survival in S and G2/M, but becomes acutely essential only during G1 phase. …

Covid-19 Convalescent Plasma Therapy Decreases Inflammatory Cytokines: A Randomized Controlled Trial, Feben Habtehyimer, Xianming Zhu, Andrew D Redd, Kelly A Gebo, Alison G Abraham, Eshan U Patel, Oliver Laeyendecker, Thomas J Gniadek, Reinaldo E Fernandez, Owen R Baker, Malathi Ram, Edward R Cachay, Judith S Currier, Yuriko Fukuta, Jonathan M Gerber, Sonya L Heath, Barry Meisenberg, Moises A Huaman, Adam C Levine, Aarthi Shenoy, Shweta Anjan, Janis E Blair, Daniel Cruser, Donald N Forthal, Laura L Hammitt, Seble Kassaye, Giselle S Mosnaim, Bela Patel, James H Paxton, Jay S Raval, Catherine G Sutcliffe, Matthew Abinante, Kevin S Oei, Valerie Cluzet, Marie Elena Cordisco, Benjamin Greenblatt, William Rausch, David Shade, Amy L Gawad, Sabra L Klein, Andrew Pekosz, Shmuel Shoham, Arturo Casadevall, Evan M Bloch, Daniel Hanley, Aaron A R Tobian, David J Sullivan

Faculty, Staff and Student Publications

This study examined the role that cytokines may have played in the beneficial outcomes found when outpatient individuals infected with SARS-CoV-2 were transfused with COVID-19 convalescent plasma (CCP) early in their infection. We found that the pro-inflammatory cytokine IL-6 decreased significantly faster in patients treated early with CCP. Participants with COVID-19 treated with CCP later in the infection did not have the same effect. This decrease in IL-6 levels after early CCP treatment suggests a possible role of inflammation in COVID-19 progression. The evidence of IL-6 involvement brings insight into the possible mechanisms involved in CCP treatment mitigating SARS-CoV-2 severity.

Chemical Catalysis Guides Structural Identification For The Major In Vivo Metabolite Of The Bet Inhibitor Jq1, Secondra Holmes, Prashi Jain, Kenneth Guzman Rodriguez, Jade Williams, Zhifeng Yu, Christian Cerda-Smith, Errol L G Samuel, James Campbell, John Michael Hakenjos, Diana Monsivais, Feng Li, Srinivas Chamakuri, Martin M Matzuk, Conrad Santini, Kevin R Mackenzie, Damian W Young

Faculty, Staff and Students Publications

The bromodomain inhibitor (+)-JQ1 is a highly validated chemical probe; however, it exhibits poor in vivo pharmacokinetics. To guide efforts toward improving its pharmacological properties, we identified the (+)-JQ1 primary metabolite using chemical catalysis methods. Treatment of (+)-JQ1 with tetrabutylammonium decatungstate under photochemical conditions resulted in selective formation of an aldehyde at the 2-position of the thiophene ring [(+)-JQ1-CHO], which was further reduced to the 2-hydroxymethyl analog [(+)-JQ1-OH]. Comparative LC/MS analysis of (+)-JQ1-OH to the product obtained from liver microsomes suggested (+)-JQ1-OH as the major metabolite of (+)-JQ1. The 2-thienyl position was then substituted to generate a trideuterated (−CD3, (+)-JQ1-D) …

Teaching-Learning-Doing (Post)Qualitative Inquiry: Relational, Digital, Nest-Making, Candice Kuby, Soern Finn Menning, Karin Murris, Jayne Osgood, Claire Walsh

School of Law, Humanities and Social Sciences

This Dream Team session is a space to explore relationally and collectively thee teaching-learning-doing of (post) qualitative inquiry. We focus our session on foureaspects as a common thread which will be offered to participants as a series ofeinterwoven intra-ventions. Throughout there will be invitations and provocations fore attendees to engage with during the session. In addition, we experiment with videoein relation to four aspects (and invite participants to as well) to explore the digital ine relation to teaching- learning-doing (post) qualitative inquiry.

Western Regional News, North American Bird Bander

North American Bird Bander

No abstract provided.

Bander's Marketplace, North American Bird Bander

North American Bird Bander

No abstract provided.

Analysis Of A Sample Of Tennessee Warblers Window-Killed During Spring Migration In Manitoba, Spencer G. Sealy

North American Bird Bander

No abstract provided.

Books, North American Bird Bander

North American Bird Bander

No abstract provided.

Pesticide Contamination And Eggshell Characteristics For Mountain Bluebirds In Colorado, Steven E. Den

North American Bird Bander

No abstract provided.

Active Netting To Capture Nesting Mourning Doves, David Edward Blockstein

North American Bird Bander

No abstract provided.

Bander's Marketplace, North American Bird Bander

North American Bird Bander

No abstract provided.

Western Regional News, North American Bird Bander

North American Bird Bander

No abstract provided.

Books, North American Bird Bander

North American Bird Bander

No abstract provided.

Noosing Adult Cormorants For Banding, Geoffrey G. Hogan

North American Bird Bander

No abstract provided.

Banders' Marketplace, North American Bird Bander

North American Bird Bander

No abstract provided.

Notice Concerning Auxiliary Marking"", North American Bird Bander

North American Bird Bander

No abstract provided.

Western Regional News, North American Bird Bander

North American Bird Bander

No abstract provided.

Return Of A Red-Eyed Vireo: A Transient Or Breeder?, Spencer G. Sealy, Heidi E. Den Haan

North American Bird Bander

No abstract provided.

Winter Recovery, In Guatemala, Of A Warbling Vireo Banded In Southern Manitoba, And An Examination Of Other Recoveries From The Wintering Range, Spencer G. Sealy

North American Bird Bander

No abstract provided.

Nesting History Of A Banded Hermaphroditic Chimney Swift, Ralph W. Dexter

North American Bird Bander

No abstract provided.

Banders' Marketplace, North American Bird Bander

North American Bird Bander

No abstract provided.

Bander's Forum, North American Bird Bander

North American Bird Bander

No abstract provided.

Capturing Breeding Male Tree Swallows With Feathers, Robert R. Cohen

North American Bird Bander

No abstract provided.

Where Do Northern (Baltimore") Orioles Spend The Winter?", Spencer G. Sealy

North American Bird Bander

No abstract provided.