Digital Commons Network^™

The Combined Immunohistochemical Expression Of Ambra1 And Sqstm1 Identifies Patients With Poorly Differentiated Cutaneous Squamous Cell Carcinoma At Risk Of Metastasis: A Proof Of Concept Study, Michael H. Alexander, William J. Cousins, Tom Ewen, Andrew P. South, Penny Lovat, Niki Stefanos

Department of Dermatology and Cutaneous Biology Faculty Papers

BACKGROUND: Cutaneous squamous cell carcinoma (cSCC) incidence continues to increase globally with, as of yet, an unmet need for reliable prognostic biomarkers to identify patients at increased risk of metastasis. The aim of the present study was to test the prognostic potential of the combined immunohistochemical expression of the autophagy regulatory biomarkers, AMBRA1 and SQSTM1, to identify high-risk patient subsets.

METHODS: A retrospective cohort of 68 formalin-fixed paraffin-embedded primary cSCCs with known 5-year metastatic outcomes were subjected to automated immunohistochemical staining for AMBRA1 and SQSTM1. Digital images of stained slides were annotated to define four regions of interest: the normal …

Pan-Cancer Proteogenomics Characterization Of Tumor Immunity, Francesca Petralia, Weiping Ma, Tomer M Yaron, Francesca Pia Caruso, Nicole Tignor, Joshua M Wang, Daniel Charytonowicz, Jared L Johnson, Emily M Huntsman, Giacomo B Marino, Anna Calinawan, John Erol Evangelista, Myvizhi Esai Selvan, Shrabanti Chowdhury, Dmitry Rykunov, Azra Krek, Xiaoyu Song, Berk Turhan, Karen E Christianson, David A Lewis, Eden Z Deng, Daniel J B Clarke, Jeffrey R Whiteaker, Jacob J Kennedy, Lei Zhao, Rossana Lazcano Segura, Harsh Batra, Maria Gabriela Raso, Edwin Roger Parra, Rama Soundararajan, Ximing Tang, Yize Li, Xinpei Yi, Shankha Satpathy, Ying Wang, Maciej Wiznerowicz, Tania J González-Robles, Antonio Iavarone, Sara J C Gosline, Boris Reva, Ana I Robles, Alexey I Nesvizhskii, D R Mani, Michael A Gillette, Robert J Klein, Marcin Cieslik, Bing Zhang, Amanda G Paulovich, Robert Sebra, Zeynep H Gümüş, Galen Hostetter, David Fenyö, Gilbert S Omenn, Lewis C Cantley, Avi Ma'ayan, Alexander J Lazar, Michele Ceccarelli, Pei Wang, Clinical Proteomic Tumor Analysis Consortium

Faculty, Staff and Student Publications

Despite the successes of immunotherapy in cancer treatment over recent decades, less than <10%-20% cancer cases have demonstrated durable responses from immune checkpoint blockade. To enhance the efficacy of immunotherapies, combination therapies suppressing multiple immune evasion mechanisms are increasingly contemplated. To better understand immune cell surveillance and diverse immune evasion responses in tumor tissues, we comprehensively characterized the immune landscape of more than 1,000 tumors across ten different cancers using CPTAC pan-cancer proteogenomic data. We identified seven distinct immune subtypes based on integrative learning of cell type compositions and pathway activities. We then thoroughly categorized unique genomic, epigenetic, transcriptomic, and proteomic changes associated with each subtype. Further leveraging the deep phosphoproteomic data, we studied kinase activities in different immune subtypes, which revealed potential subtype-specific therapeutic targets. Insights from this work will facilitate the development of future immunotherapy strategies and enhance precision targeting with existing agents.

Managing Saline And Sodic Soils And Irrigation Water, Burdette Barker, Grant Cardon, Matt Yost, Melanie Stock, Earl Creech, Jody Gale

All Current Publications

Salt is an important factor in plant and soil management. Excessive salt concentrations in soil can cause water to be less available to plants because of the osmotic forces of salt in the soil water. Excessive concentrations of different ions can also be toxic to plants. In agricultural soils and irrigation water, salts are typically described in two ways: (1) total salt concentration or salinity, and (2) sodicity, or the concentration of sodium relative to other cations (positively charged atoms or compounds). Understanding the effect of both total salinity and sodicity on plants and soils, along with management and reclamation …

Factors Influencing Local Control After Mr-Guided Stereotactic Body Radiotherapy (Mrgsbrt) For Adrenal Metastases, Rupesh Kotecha, Tugce Kutuk

All Publications

Purpose: Stereotactic body radiotherapy (SBRT) is an effective treatment for adrenal gland metastases, but it is technically challenging and there are concerns about toxicity. We performed a multi-institutional pooled retrospective analysis to study clinical outcomes and toxicities after MR-guided SBRT (MRgSBRT) using for adrenal gland metastases.

Methods and materials: Clinical and dosimetric data of patients treated with MRgSBRT on a 0.35 T MR-Linac at 11 institutions between 2016 and 2022 were analyzed. Local control (LC), local progression-free survival (LPFS), distant progression-free survival (DPFS) and overall survival (OS) were estimated using Kaplan-Meier method and log-rank test.

Results: A total of 255 …

Genetic Algorithm For Automated Parameterization Of Network Hamiltonian Models Of Amyloid Fibril Formation, Gianmarc Grazioli, Andy Tao, Inika Bhatia, Patrick Regan

Faculty Research, Scholarly, and Creative Activity

The time scales of long-time atomistic molecular dynamics simulations are typically reported in microseconds, while the time scales for experiments studying the kinetics of amyloid fibril formation are typically reported in minutes or hours. This time scale deficit of roughly 9 orders of magnitude presents a major challenge in the design of computer simulation methods for studying protein aggregation events. Coarse-grained molecular simulations offer a computationally tractable path forward for exploring the molecular mechanism driving the formation of these structures, which are implicated in diseases such as Alzheimer’s, Parkinson’s, and type-II diabetes. Network Hamiltonian models of aggregation are centered around …

Pd-1 Inhibition Combined With Intensity-Modulated Radiotherapy, To Better Serve Patients With Retroperitoneal Lymph Node Metastases From Gastrointestinal Cancer, Michael Chuong

All Publications

Background: Gastrointestinal (GI) cancer is the most frequent kind of cancer to involve the retroperitoneal lymph nodes (RPLNs). Radiotherapy (RT) is common treatment of RPLN metastases in patients with GI cancer, while RT is local. Meanwhile, most patients have extra-retroperitoneal metastases. Immunotherapy plus RT have showed effective in advanced non-small cell lung cancer. However, whether the combination therapy is effective on GI cancer with RPLN metastases. In our study, we would estimate the effect of programmed death-1 (PD-1) inhibition in association with intensity modulated radiation therapy (IMRT).

Methods: Metastatic GI cancer patients with RPLN who were treated at a single …

Vkorc1 Polymorphisms And Complete Resistance To Vitamin K Antagonists: About Two Cases., Ilham Benyamna, Houda El Fissi, Fadoua Bouzid, Abdelhamid El Mousadik, Najat Alif

BioMedicine

Complete resistance to vitamin K antagonists is a rare but serious. It can complicate therapeutic management, especially when direct oral anticoagulants cannot be used. Some single mutations in the VKORC1 gene have been identified in patients partially or completely resistant to vitamin K antagonists. We report the cases of two women in their fifties who presented an unexplained peripheral venous thrombosis. The aetiological assessment did not show any abnormalities. Genetic testing showed that both patients had the VKORC1 5417GG genotype. The VKORC1 3673 genotype was GG in case 1 and GA in case 2. The two patients showed complete resistance …

Ethanol Leaf Extracts Of Anogeissus Leiocarpus In Antioxidants And Hepatotoxic Effects Of Escherichia Coli Infected Swiss Mice, Fred Coolborn Akharaiyi, Chioma Bertha Ehis-Eriakha, Peter Taiwo Olagbemide, Stephen Eromosele Akemu

BioMedicine

Introduction: Diseases caused by bacteria can be managed with medicinal plants with rightful dosage that will not affect body physiology and organs.

Aim: This research aimed to evaluate the antioxidants and the effects of Anogeisus leiocarpus on liver function.

Materials and Methods: Ethanol leaf extracts were processed for antioxidants and hepatotoxic effects using animal models. Group one (negative control) was given access to water and regular feed, group two (positive control) was dosed with 107 CFU/ml of Escherichia coli O157:H7, and groups 3-6 were dosed with 107 E. coli O157:H7 for 3 days and treated with extract concentrations of 12, …

Biomedicines In Longevity And Aging The Quest To Resist Biological Decline, Raymond D D Raymond

BioMedicine

Aging is considered part of the natural process of life, however in recent years medical literature has started to show that specific facets of aging are beginning to be understood and those specific factors may even be considered preventable with various measures as this paper will demonstrate. Aging is also considered the number one cause of poor quality of life, disease, disability, and death, so the importance of understanding the aging process and how to control certain aspects of it cannot be underestimated when age related suffering is factored in. The causes of aging are now becoming well understood, and …

Gender, Graduate School Stage, And The Impostor Phenomenon, John-Scott B. Kelley, Angela T. Barlow

Journal of Graduate Education Research

The impostor phenomenon (IP) includes five central factors: (a) a sense of fraudulence or phoniness; (b) a fear of failure and discovery; (c) compensatory perfectionism (i.e., procrastination and/or over-preparation); (d) interpersonal anxiety; and (e) externalized success and/or discounted positive feedback. After the final stage, the process starts over with reinforced vigor, creating a self-reinforcing cycle in which success is associated with psychological suffering. IP was initially used to describe the reports of high-achieving women, but recent studies have shown that IP is experienced across genders. Additionally, while graduate school is an achievement-oriented environment with many characteristics that could promote IP, …

Psychosocial Aspects Of Academic Life, Kimberley Sartain

Journal of Graduate Education Research

No abstract provided.

Full Issue

Journal of Graduate Education Research

No abstract provided.

Attitudes Of Intercollegiate Athletes Toward Transgender Individuals And Transgender Athletic Participation, Austin R. Anderson, Eric Knee, Alison Fridley

Journal of Issues in Intercollegiate Athletics

Debate around the participation of transgender athletes in competitive sport has been increasing recently. These debates, and the discourse surrounding transgender athletes, have had wide ranging consequences, including changes to gender participation policies by sport governing bodies. While policy changes are being made, little data exists around the attitudes of athletes on the participation of transgender persons. This study explores the attitudes of intercollegiate student-athletes on transgender individuals and transgender collegiate athletic participation, using a sample of three collegiate athletic programs. Findings suggest that attitudes towards transgender persons and political ideology are significant factors in the acceptance of transgender athletic …

Medical School Watercooler Newsletter - February 28, 2024, Lindsey Lyle

Watercooler Newsletter

This is the February 28, 2024 edition of the Frederick P. Whiddon College of Medicine's newsletter - Watercooler.

Contents Include:

• Meet a Med Student: Emily Cleveland
• MAA to host Student Career Exploration Mixer
• Elliott joins microbiology and immunology faculty

Educating The Whole Child: The Transformative Power Of Self-Efficacy, Hannah Wallenta

Graduate Teacher Education

This paper examined research on the teaching strategies that impact self-efficacy and student achievement. This topic was explored through a multitude of quantitative and qualitative studies which provided a comprehensive understanding of overall benefits of increasing self-efficacy, teachers' roles in development, and multitude of strategies used in the classroom to support teachers in creating student learning experiences that promote self-efficacy. This research goes beyond what structures are needed for student success and delves into the methods used to support the whole child from a psychological perspective that prepares them for success outside of the classroom. Further research is needed to …

Commonwealth Times 2024-02-28

Commonwealth Times, 1969-

No abstract provided.

Come, Let Us Reason Together, Hon. Kent A. Jordan, James J. Brudney

Jurist in Residence Lectures

In his lecture, Judge Kent A. Jordan emphasizes the critical importance of civil discourse in addressing complex legal and social issues. He explores the dangers of abandoning reasoned debate for heated rhetoric and the negative impacts this can have on the legal profession and society at large.

2024 February 28 - Undergraduate Curriculum Council Minutes, Undergraduate Curriculum Council, East Tennessee State University

Undergraduate Curriculum Council Minutes

No abstract provided.

Ethics And Best Practices Of Studying Contemporary Human Populations, Mayowa Adegboyega, Xinjun Zhang

Human Biology Open Access Pre-Prints

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The Hbcu Experience: Why I Chose My Hbcu And How It Helped Me Prepare For My Career, Illinois Mathematics And Science Academy, Angela Richardson

Educational Panels

Panel/Event Coordinator: Angela Richardson

Moderated by: Dr. Anita White

Panelists:

• Justin Alfred : Southern University and A&M College, Baton Rouge. Civil Engineering / Computer science. Lockheed Martin.
• Joseph Bertrand ‘20 : Senior at North Carolina Agricultural and Technical State University, Greensboro, NC. Mechanical Engineering.
• Darryl Burrell Jr. : Morgan State University, Baltimore, MD.Civil Engineering. City of Houston,Houston Public Works.
• Dr. Garry J. Kennebrew Jr. ‘04 : Morehouse College, Atlanta BS Biology Loyola University Chicago Stritch School of Medicine.Doctorate in Medicine.
• Jaida Lewis ‘15 : Texas Southern University Chemistry. Owner, Poised Lips by Jai Program Coordinator, CVS Health.
• Ja’Nae …

Physical Properties Of Odorants Affect Behavior Of Trained Detection Dogs During Close-Quarters Searches, Daniel Mejia, Lydia Burnett, Nicholas Hebdon, Peter Stevens, Alexis Shiber, Clay Cranston, Lauryn Degreeff, Lindsay D. Waldrop

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

Trained detection dogs have a unique ability to find the sources of target odors in complex fluid environments. How dogs derive information about the source of an odor from an odor plume comprised of odorants with different physical properties, such as diffusivity, is currently unknown. Two volatile chemicals associated with explosive detection, ammonia (NH₃, derived from ammonium nitrate-based explosives) and 2-ethyl-1-hexanol (2E1H, associated with composition C4 plastic explosives) were used to ascertain the effects of the physical properties of odorants on the search behavior and motion of trained dogs. NH₃ has a diffusivity 3.6 times that of …

Stat5 Induces Androgen Receptor (Ar) Gene Transcription In Prostate Cancer And Offers A Druggable Pathway To Target Ar Signaling, Cristina Maranto, Lavannya Sabharwal, Vindhya Udhane, Samuel P. Pitzen, Braedan Mccluskey, Songyan Qi, Christine O'Connor, Savita Devi, Scott Johnson, Kenneth Jacobsohn, Anjishnu Banerjee, Kenneth A. Iczkowski, Liang Wang, Scott M. Dehm, Marja T. Nevalainen

Department of Pharmacology, Physiology, and Cancer Biology Faculty Papers

Androgen receptor (AR) drives prostate cancer (PC) growth and progression, and targeting AR signaling is the mainstay of pharmacological therapies for PC. Resistance develops relatively fast as a result of refueled AR activity. A major gap in the field is the lack of understanding of targetable mechanisms that induce persistent AR expression in castrate-resistant PC (CRPC). This study uncovers an unexpected function of active Stat5 signaling, a known promoter of PC growth and clinical progression, as a potent inducer of AR gene transcription. Stat5 suppression inhibited AR gene transcription in preclinical PC models and reduced the levels of wild-type, mutated, …

The Clash Of Sustainable Development Goals With The Reality In Egypt: The Role Of The Media On The Perception Of Clean Energy Implementation., Nourhan Salah Hamed Ebrahim

Theses and Dissertations

Media has promoted sustainable development goals as essential for a country's social, economic, environmental, and political development. However, the SDGs may collide with the reality and impede development due to certain circumstances and challenges that might hinder the country’s ability to achieve these goals the same way they planned for. Besides, such challenges are not being covered in the media agenda, addressed by the government, or even been tackled in previous research. Thus, this study aims to examine whether there is a conflict between the SDGs and the reality in Egypt, focusing on SDG 7 (Clean Energy) by analyzing the …

Deeply Mourning Prof. Allen Joseph Bard—The Worldwide Famous Electrochemist, Chinese Society Of Electrochemistry (Cse)

Journal of Electrochemistry

No abstract provided.

Lichen Ecosystem Services And A Study From The Nj Pine Barrens, Gregory Turner

Sustainability Research & Practice Seminar Presentations

Professor Greg Turner of the WCU Biology Department presents Lichen Ecosystem Services and a Study from the New Jersey Pine Barrens.

February 28, 2024, Faculty, Illinois Wesleyan University

2023-2024

No abstract provided.

Resolution Of Respect: Conrad Alan Istock (1936–2023), Steven Hecht Orzack, Julia Bell, Judith Bronstein, William Etges, Shripad Tuljakpurkar

Biological Sciences Faculty Publications and Presentations

Conrad Istock was an important contributor to the emerging field of evolutionary ecology starting in the 1960s. His best-known work in this area blended theory and field and laboratory studies of pitcher-plant mosquitoes. Conrad died March 8, 2023, in Ithaca, New York, USA.

Hmzdupfinder: A Robust Computational Approach For Detecting Intragenic Homozygous Duplications From Exome Sequencing Data, Haowei Du, Zain Dardas, Angad Jolly, Christopher M Grochowski, Shalini N Jhangiani, He Li, Donna Muzny, Jawid M Fatih, Gozde Yesil, Nursel H Elçioglu, Alper Gezdirici, Dana Marafi, Davut Pehlivan, Daniel G Calame, Claudia M B Carvalho, Jennifer E Posey, Tomasz Gambin, Zeynep Coban-Akdemir, James R Lupski

Faculty, Staff and Students Publications

Homozygous duplications contribute to genetic disease by altering gene dosage or disrupting gene regulation and can be more deleterious to organismal biology than heterozygous duplications. Intragenic exonic duplications can result in loss-of-function (LoF) or gain-of-function (GoF) alleles that when homozygosed, i.e. brought to homozygous state at a locus by identity by descent or state, could potentially result in autosomal recessive (AR) rare disease traits. However, the detection and functional interpretation of homozygous duplications from exome sequencing data remains a challenge. We developed a framework algorithm, HMZDupFinder, that is designed to detect exonic homozygous duplications from exome sequencing (ES) data. The …

Bellwethers Of Change: Population Modelling Of North Pacific Humpback Whales From 2002 Through 2021 Reveals Shift From Recovery To Climate Response, Ted Cheeseman, Jay Barlow, Jo Marie Acebes, Katherina Audley, Lars Bejder, Catlin Birdsall, O. S. Bracamontes, Amanda L. Bradford, Josie Byington, John Calambokidis, Rachel Cartwright, Jen Cedarleaf, A. J. Garcia Chavez, Jens Currie, R. C. De Castro, Joelle De Weerdt, Nicole Doe, Thomas Doniol-Valcroze, Karina Dracott, Olga Filatova, Rachel Finn, Kiirsten R. Flynn, John Ford, Astrid Frisch-Jordan, Chris Garbriele, Beth Goodwin, Craig Hayslip, Jackie Hildering, Marie C. Hill, Jeff K. Jacobsen

Faculty, Staff, and Students

For the 40 years after the end of commercial whaling in 1976, humpback whale populations in the North Pacific Ocean exhibited a prolonged period of recovery. Using mark–recapture methods on the largest individual photo-identification dataset ever assembled for a cetacean, we estimated annual ocean-basin-wide abundance for the species from 2002 through 2021. Trends in annual estimates describe strong post-whaling era population recovery from 16 875 (± 5955) in 2002 to a peak abundance estimate of 33 488 (± 4455) in 2012. An apparent 20% decline from 2012 to 2021, 33 488 (± 4455) to 26 662 (± 4192), suggests the …

Automated Workflow For Redox Potentials And Acidity Constants Calculations From Machine Learning Molecular Dynamics, Feng Wang, Jun Cheng

Journal of Electrochemistry

Redox potentials and acidity constants are key properties for evaluating the performance of energy materials. To achieve computational design of new generation of energy materials with higher performances, computing redox potentials and acidity constants with computational chemistry have attracted lots of attention. However, many works are done by using implicit solvation models, which is difficult to be applied to complex solvation environments due to hard parameterization. Recently, ab initio molecular dynamics (AIMD) has been applied to investigate real electrolytes with complex solvation. Furthermore, AIMD based free energy calculation methods have been established to calculate these physical chemical properties accurately. However, …