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If1 Promotes Cellular Proliferation And Inhibits Oxidative Phosphorylation In Mouse Embryonic Fibroblasts Under Normoxia And Hypoxia, Lothar Lauterboeck, Sung Wook Kang, Donnell White, Rong Bao, Parnia Mobasheran, Qinglin Yang

School of Graduate Studies Faculty Publications

ATP synthase inhibitory factor subunit 1 (IF1) is an inhibitory subunit of mitochondrial ATP synthase, playing a crucial role in regulating mitochondrial respiration and energetics. It is well-established that IF1 interacts with the F1 sector of ATP synthase to inhibit the reversal rotation and, thus, ATP hydrolysis. Recent evidence supports that IF1 also inhibits forward rotation or the ATP synthesis activity. Adding to the complexity, IF1 may also facilitate mitophagy and cristae formation. The implications of these complex actions of IF1 for cellular function remain obscure. In the present study, we found that IF1 expression was markedly upregulated in hypoxic …

Quantitative Shotgun Proteomic Analysis Of Bacteria After Overexpression Of Recombinant Spider Miniature Spidroin, Masp1, Kathryn Randene, J. Alexander H. Mendoza, Michael Ysit, Craig Vierra

Pacific Faculty Work

Spider silk has extraordinary mechanical properties, displaying high tensile strength, elasticity, and toughness. Given the high performance of natural fibers, one of the long-term goals of the silk community is to manufacture large-scale synthetic spider silk. This process requires vast quantities of recombinant proteins for wet-spinning applications. Attempts to synthesize large amounts of native size recombinant spidroins in diverse cell types have been unsuccessful. In these studies, we design and express recombinant miniature black widow MaSp1 spidroins in bacteria that incorporate the N-terminal and C-terminal domain (NTD and CTD), along with varying numbers of codon-optimized internal block repeats. Following spidroin …

March 21, 2024, The Daily Mississippian

Daily Mississippian (all digitized issues)

No abstract provided.

The Difference Between Males And Females In First Language Acquisition, Haya Alotaibi

Sociology Between the Gaps: Forgotten and Neglected Topics

No abstract provided.

Women’S Lack Of Top Leadership Positions In The Public Sector Despite Having Similar Qualifications As Men, Ashley Rodgers

Walden Dissertations and Doctoral Studies

No abstract provided.

Investigating The Use Of Finerenone In Children With Chronic Kidney Disease And Proteinuria: Design Of The Fiona And Open-Label Extension Studies., Franz Schaefer, Giovanni Montini, Hee Gyung Kang, Johan Vande Walle, Joshua Zaritsky, Michiel F. Schreuder, Mieczyslaw Litwin, Andrea Scalise, Helen Scott, James Potts, Pablo Iveli, Stefanie Breitenstein, Bradley A. Warady

Manuscripts, Articles, Book Chapters and Other Papers

INTRODUCTION: Proteinuria is a modifiable risk factor for chronic kidney disease (CKD) progression in children. Finerenone, a selective, non-steroidal, mineralocorticoid receptor antagonist (MRA) has been approved to treat adults with CKD associated with type 2 diabetes mellitus (T2DM) following results from the phase III clinical trials FIDELIO-DKD (NCT02540993) and FIGARO-DKD (NCT02545049). In a pre-specified pooled analysis of both studies (N = 13,026), finerenone was shown to have an acceptable safety profile and was efficacious in decreasing the risk of adverse kidney and cardiovascular outcomes and of proteinuria.

OBJECTIVE: FIONA and the associated open-label extension (OLE) study aim to demonstrate that …

Cataloging The Phylogenetic Diversity Of Human Bladder Bacterial Isolates, Jingjie Du, Mark Khemmani, Thomas Halverson, Adriana Ene, Roberto Limeira, Lana Tinawi, Baylie R. Hochstedler-Kramer, Melline Fontes Noronha, Catherine Putonti, Alan J. Wolfe

Biology: Faculty Publications and Other Works

Background: Although the human bladder is reported to harbor unique microbiota, our understanding of how these microbial communities interact with their human hosts is limited, mostly owing to the lack of isolates to test mechanistic hypotheses. Niche-specific bacterial collections and associated reference genome databases have been instrumental in expanding knowledge of the microbiota of other anatomical sites, such as the gut and oral cavity. Results: To facilitate genomic, functional, and experimental analyses of the human bladder microbiota, we present a bladder-specific bacterial isolate reference collection comprising 1134 genomes, primarily from adult females. These genomes were culled from bacterial isolates obtained …

Mapping Sca1 Regional Vulnerabilities Reveals Neural And Skeletal Muscle Contributions To Disease, Lisa Duvick, W Michael Southern, Kellie A Benzow, Zoe N Burch, Hillary P Handler, Jason S Mitchell, Hannah Kuivinen, Udaya Gadiparthi, Praseuth Yang, Alyssa Soles, Carrie A Sheeler, Orion Rainwater, Shannah Serres, Erin B Lind, Tessa Nichols-Meade, Yun You, Brennon O'Callaghan, Huda Y Zoghbi, Marija Cvetanovic, Vanessa C Wheeler, James M Ervasti, Michael D Koob, Harry T Orr

Duncan NRI Faculty and Staff Publications

Spinocerebellar ataxia type 1 (SCA1) is a fatal neurodegenerative disease caused by an expanded polyglutamine tract in the widely expressed ataxin-1 (ATXN1) protein. To elucidate anatomical regions and cell types that underlie mutant ATXN1-induced disease phenotypes, we developed a floxed conditional knockin mouse (f-ATXN1146Q/2Q) with mouse Atxn1 coding exons replaced by human ATXN1 exons encoding 146 glutamines. f-ATXN1146Q/2Q mice manifested SCA1-like phenotypes including motor and cognitive deficits, wasting, and decreased survival. Central nervous system (CNS) contributions to disease were revealed using f-ATXN1146Q/2Q;Nestin-Cre mice, which showed improved rotarod, open field, and Barnes maze performance by 6-12 weeks of age. In contrast, …

A Prognostic Model For Schistosoma Japonicum Infection-Associated Liver Hepatocellular Carcinoma: Strengthening The Connection Through Initial Biological Experiments, Shuyan Sheng, Bangjie Chen, Ruiyao Xu, Yanxun Han, Deshen Mao, Yuerong Chen, Conghan Li, Wenzhuo Su, Xinyang Hu, Qing Zhao, Scott Lowe, Yuting Huang, Wei Shao, Yong Yao

Student Publications

Background: Numerous studies have shown that Schistosoma japonicum infection correlates with an increased risk of liver hepatocellular carcinoma (LIHC). However, data regarding the role of this infection in LIHC oncogenesis are scarce. This study aimed to investigate the potential mechanisms of hepatocarcinogenesis associated with Schistosoma japonicum infection.

Methods: By examining chronic liver disease as a mediator, we identified the genes contributing to Schistosoma japonicum infection and LIHC. We selected 15 key differentially expressed genes (DEGs) using weighted gene co-expression network analysis (WGCNA) and random survival forest models. Consensus clustering revealed two subgroups with distinct prognoses. Least Absolute Shrinkage and Selection …

Gut Microbiota Composition Is Associated With The Efficacy Of Delta-24-Rgdox In Malignant Gliomas, Natalie M Meléndez-Vázquez, Teresa T Nguyen, Xuejun Fan, Andrés R López-Rivas, Juan Fueyo, Candelaria Gomez-Manzano, Filipa Godoy-Vitorino

Faculty, Staff and Student Publications

Glioblastoma, the most common primary brain tumor, has a 6.8% survival rate 5 years post diagnosis. Our team developed an oncolytic adenovirus with an OX-40L expression cassette named Delta-24-RGDOX. While studies have revealed the interaction between the gut microbiota and immunotherapy agents, there are no studies linking the gut microbiota with viroimmunotherapy efficacy. We hypothesize that gut bacterial signatures will be associated with oncolytic viral therapy efficacy. To test this hypothesis, we evaluated the changes in gut microbiota in two mouse cohorts: (1) GSC-005 glioblastoma-bearing mice treated orally with indoximod, an immunotherapeutic agent, or with Delta-24-RGDOX by intratumoral injection and …

Anti-Inflammatory, Antipyretic, And Analgesic Potential Of Chitin And Chitosan Derived From Cockroaches (Periplaneta Americana) And Termites, Khushbakht Asad, Sumaira Shams, Eliana Ibáñez-Arancibia, Patricio R De Los Ríos-Escalante, Farhad Badshah, Farooq Ahmad, Muhammad Salman Khan, Asar Khan

Faculty, Staff and Student Publications

The chitin and chitosan biopolymers are extremely valuable because of their numerous industrial and pharmacological uses. Chitin and chitosan were extracted from the exoskeleton of Periplaneta americana (cockroaches) and termites using various acid and alkali techniques. The extraction process involves an initial demineralization step, during which integument dry powder was subjected to 500 mL (2.07 mol/L) of concentrated HCl at 100 degrees Celsius for 30 min, followed by meticulous rinsing with distilled water to restore the pH to its baseline. Deproteinization was conducted at 80 degrees Celsius using 500 mL (1 mol/L) of NaOH solution, which was repeated for 24 …

Gut Microbiota Composition Is Associated With The Efficacy Of Delta-24-Rgdox In Malignant Gliomas, Natalie M Meléndez-Vázquez, Teresa T Nguyen, Xuejun Fan, Andrés R López-Rivas, Juan Fueyo, Candelaria Gomez-Manzano, Filipa Godoy-Vitorino

Faculty, Staff and Student Publications

Glioblastoma, the most common primary brain tumor, has a 6.8% survival rate 5 years post diagnosis. Our team developed an oncolytic adenovirus with an OX-40L expression cassette named Delta-24-RGDOX. While studies have revealed the interaction between the gut microbiota and immunotherapy agents, there are no studies linking the gut microbiota with viroimmunotherapy efficacy. We hypothesize that gut bacterial signatures will be associated with oncolytic viral therapy efficacy. To test this hypothesis, we evaluated the changes in gut microbiota in two mouse cohorts: (1) GSC-005 glioblastoma-bearing mice treated orally with indoximod, an immunotherapeutic agent, or with Delta-24-RGDOX by intratumoral injection and …

Silvopastoral Agroforestry In Upland And Lowland Uk Grassland: Tree Growth And Animal Performance, W R. Eason, R Lavender, R O. Clements, C Duller, E Gill, M Hislop

IGC Proceedings (1977-2023)

Trees, individually protected from herbivore damage using plastic shelters, were planted at two densities (100 and 400 stems/ha) into sheepgrazed pasture in upland and lowland UK grassland sites in 1988. Tree and animal performance were compared with conventional forestry (no sheep) and pasture (no tree) systems. Effects on tree growth and survival are highly species and site dependent although some treatment effects did emerge. Tree shelters encouraged rapid early height growth compared to forestry controls although in some cases tree form was also adversely affected. Generally tree performance within agroforestry treatments was better at the higher planting density. Eight years …

Use Of Annual Self-Reseeding Legumes In An Oak Forest In Central Italy, P Talamucci, G Argenti, A Pardini, S Piemontese, N Stagliano

IGC Proceedings (1977-2023)

A trial has been carried out for three years in an oak coppice geometrically thinned out of Mediterranean Central Italy, about the functioning of a pastoral system made by four different resources: native pasture, subterranean clover (Trifolium brachycalycinum Katzn. et Morley) in pure stand, strips thinned out and firebreaks improved with oversown subclover, all grazed by sheep. Subclover increased the forage yield and improved the palatabilty of the pasture and the regularity of grazing; the higher biomass intake by animals reduced the quantity of dried biomass in summer and contributed to keep low either fire hazards or flame diffusion …

The Effects Of Shelterbelts On Adjacent Pastures And Soils In A Temperate Climate, A G. Gillingham, M F. Hawke

IGC Proceedings (1977-2023)

Two trials were conducted to differentiate the direct (exposure) from the indirect (modified soil fertility due to nutrient transfer by grazing animals) effects of farm shelterbelts on associated pasture growth.Soil from close to “unmanaged”shelterbelts with dense shelter to ground level had relatively high potassium (K) levels and, in a glasshouse situation, provided more pasture growth than soil from further distances, or from adjacent to “managed” shelterbelts. Pasture grown in boxes of a common soil implanted at increasing distances from a shelterbelt also produced highest growth rates close to shelter. These results generally explain the pattern of resident pasture growth, except …

Temperate Pasture And Sheep Performance Under Radiata Pine And In Open Pasture, K M. Pollock, R J. Lucas, D B. Pownall, S E. Thomson

IGC Proceedings (1977-2023)

Forage production from years 4 to 6 of an agroforestry system at 400-600 trees per ha and pasture alone were compared for a temperate sub-humid environment. Forage production was best for lucerne pasture followed by phalaris/clover and cocksfoot/clover, and least for ryegrass/clover and the ryegrass only pastures. Total pasture production in the agroforest relative to the open pasture was from 10% more for phalaris to 20% less for lucerne. Sheep carrying capacity over all pastures was 14.6 sheep/ha in the open and 11.8 sheep/ha in the agroforest and varied by ±15% according to the pasture types.

Grazing Management Of Tagasaste (Chamaecytisus Proliferus) For Sheep And Cattle Production In Southern Australia, N J. Edwards, G M. Allen, D M. Mcneill, C M. Oldham

IGC Proceedings (1977-2023)

Direct grazing of hedgerows of tagasaste (Chamaecytisus proliferus) by sheep or cattle appear to be very robust systems. Tagasaste persists under a continuous grazing regime with cattle such that plant regrowth maintained between 5 and 10 cm in length produces in excess of 215 kg of animal liveweight/ha/year. This level of production is also sustained within a rotational grazing regime. Under both grazing systems cattle production within a year is highly seasonal, with liveweight gains from young cattle peaking at 1.0-1.5 kg/head/day in winter and spring, but dropping to maintenance only by late summer-autumn. Sheep, like cattle, can …

Shrub Palatability To Rusa Deer (Cervus Timorensis Russa) In New Caledonia, C Corniaux, S Le Bel, J M. Sarrailh

IGC Proceedings (1977-2023)

The objective of this study was to determine a palatability scale of five shrub legumes to rusa deer during the dry season in New Caledonia. Acacia ampliceps and Samanea saman remain low in acceptability. Gliricidia sepium is more palatable but quite less than Leucaena leucocephala (native cultivar) and Calliandra calothyrsus (San Ramon). Therefore, since the regression of Leucaena leucocephala population, Calliandra calothyrsus could be very promising to replace it in the deer diet. On the other hand, Acacia ampliceps seems to be the most interesting shrub legume to plant in the west coast, where soil erosion, due to successive droughts …

Soil Nutrient Redistribution Pattern About The Tree In A Silvopastoral System, L C. Nwaigbo, H G. Miller, A R. Sibbald, G Hudson

IGC Proceedings (1977-2023)

The objective of this paper is to report the effect of animal-tree interactions on soil nutrient redistribution pattern in a grazed silvopastoral experiment site at Glensaugh, in NE Scotland. Scots pine (Pinus sylvestris L) tree species were planted in square lattice arrangements at 5 m x 5 m, spacing (400 stems/ha) on plots replicated over three blocks in Randomized Complete Block design on a predominantly rye grass (Lolium perenne L) pasture which was grazed by sheep yearly from April to October. Included in the design were grazed pasture plots without trees (Control). Soil samples were collected from around …

Trees For Shelter: The Implications In Agroforestry System, L C. Nwaigbo, A R. Sibbald, G Hudson

IGC Proceedings (1977-2023)

The objective of this study was to determine the horizontal and vertical variations in soil penetration resistance (PR) observed at tree-scale in silvopastoral plots that were grazed by sheep with and without trees. Sycamore trees (Acer pseudoplatanus L) were planted in the spring of 1988 at 10 m x 10 m spacing (100 stems/ha) at Glensaugh NE of Scotland on plots replicated over three blocks in Randomized Complete Block design on a predominantly rye grass (Lolium perenne L) pasture. Included in the design were pasture plots without trees (Control). The experiment is grazed by sheep yearly from April to …

A Silvopastoral System In The North Atlantic Zone Of Costa Rica: Combining Indigenous Timber Species With Dairy Pasture Swards, A Moulaert- Quiros, J P. Mueller, M Villarreal, R Piedra, L Villalobos

IGC Proceedings (1977-2023)

The objective of this study was to design, establish and monitor a silvo pastoral experiment on a dairy farm in the northern Atlantic region of Costa Rica. Indigenous timber species, Vochysia guatemalensis and Hyeronima alchorneoides were used together with or without the tropical pasture legume, Arachis pintoi in a split plot design with a 4 x 2 x 2 factorial arrangement. First year establishment was good for the tree component (2.3 to 10 % mortality) but poor for A. pintoi (4 to 5 % of sward). Poor legume establishment was attributed to lax grazing management and excess competition from existing …

Productivity Of Three Tree Legumes Grazed By Cattle, R C. Gutterridge

IGC Proceedings (1977-2023)

Three tree legumes Albizia chinensis, Leucaena leucocephala K 636 and Tipuana tipu were grown in conjunction with the grass Brachiaria decumbens and grazed by weaner cattle at a stocking rate of 2.5 animals per hectare for a total of 447 days. Albizia was best adapted to this acidic, poorly drained site in south east Queensland giving the highest yields of edible dry matter, high survival rate and moderate liveweight gains of 0.45 kg/head/day. The productivity of L. leucocephala K 636 was always poor but it gave the highest liveweight gains of all treatments in the first 280 days of grazing, …

Morphological And Physiological Response Of Planeleaf Willow (Salix Planifolia Pursh.) To Simulated Browsing, L Xu, J L. Dodd, M A. Smith, Q D. Skinner, W A. Laycock

IGC Proceedings (1977-2023)

Morphological and physiological responses of planeleaf willow (Salix planefolia Pursh.) to simulated browsing were studied under controlled conditions. The treatments consisted of every combination of three clipping intensities (30%, 60% and 90% of current twigs length removal) and three clipping timings (late winter, early spring and mid-summer). Increased clipping intensity stimulated bud activation; increased total leaf area and leaf size and increased the length of current year’s twigs. Mid-summer browsing increased the total number of leaves, the number of current twigs and decreased the length of current twigs. Higher browsing intensity resulted in higher photosynthetic rate of recently matured …

Psyllid Resistance In The Leucaena Genus, B F. Mullen, H M. Shelton, F Gabunada, W W. Stur

IGC Proceedings (1977-2023)

Leucaena species varied from highly susceptible to highly resistant in their response to the psyllid insect (Heteropsylla cubana) at 2 sites, a high psyllid environment at Brisbane, Australia and a moderate psyllid environment at Los Baños, Philippines. L. leucocephala was the most susceptible species. There was considerable intraspecific variation in psyllid resistance within L. collinsii, L. diversifolia and L. pallida. Plant reponse to psyllid challenge between environments was highly correlated but not linear. The higher psyllid challenge environment at Brisbane permitted greater discrimination between species and fewer Leucaena species were ranked as psyllid resistant.

Fodder Production Of Multipurpose Trees In A Maize Farming System Of Subhumid Southern Africa, B H. Dzowela, L Hove, P L. Mafongoya

IGC Proceedings (1977-2023)

Ways to integrate fodder production into maize production systems under Southern Africa’s subhumid conditions have been studied. One way involves the planting of tree and grass fodder hedges along soil conservation contour-bunds in maize fields. Management factors that influence the productivity of maize and fodder components in the system are fodder spp., side-pruning and deferment of wet season cutting. The other option involves interplanting tree fodders with maize through the use of a coppicing tree such as Acacia angustissima. Practical applications of these methods in mixed crop-livestock production systems are discussed.

Differences In Chemical Composition Among Provenances Of Browse Species In A Subhumid Environment: Relation To Use As Supplements, B H. Dzowela, L Hove, P L. Mafongoya

IGC Proceedings (1977-2023)

Studies were initiated in a subhumid Southern African environment to assess the chemical composition and nutritive value of Acacia angustissima (Mill.) Kuntze, Calliandra calothyrsus Meiss. and Leucaena species. The objective was to determine variability in crude protein (CP) concentration, acid detergent fibre (ADF), neutral detergent fibre (NDF), and soluble and insoluble proanthocyanidins from fodder samples of species, subspecies and provenances. A wide range was found in these parameters. The implications of these chemical composition factors, especially proanthocyanidins, on the use of these browse fodders in livestock feeding systems are discussed.

Tree Forages As Nitrogen Source For Ruminants In Sahelian Agro-Silvipastoral Systems, S T. Fall, B M. Doreau

IGC Proceedings (1977-2023)

Tree forages contribute up to 80% DM and 100% nitrogen content in the ruminants diet in the Sahel during the dry season. However, crude protein (CP) concentration in browse within a wide range and variation factors remain to be elucidated for better integration of browses in ruminants feeding systems. Variation factors of CP content and in vivo digestibility of four Sahelian browse species were investigated to appreciate the potential as nitrogen sources in Sahelian ruminant feeding systems. Seasonal variations in nitrogen content were evaluated in different plants part of A. raddiana, B. rufescens, B. aegyptiaca and G. senegalensis harvested in …

Introduction Of Multipurpose Tree Species For Improving The Productivity Of Grasslands In The Tropics, N G. Hegde, C Sall, T Nolan, J Connolly

IGC Proceedings (1977-2023)

To improve the productivity of the Sahelian pastures, 10 different tree species were introduced and cattle, sheep and goats were allowed to graze. After a year of establishment, survival rate of different species ranged from 6% to 68%. Acacia senegal, Acacia seyal, Acacia nilotica, Combretum aculeata and Prosopis cineraria performed well and the impact of browsing by goats was severe.

Evaluation Of Animal Productivity In A Sustainable Grazing System Based On The Use Of Trees In Association With Pastures, J M. Iglesias Gomez

IGC Proceedings (1977-2023)

Young bulls of different breeds ( Zebu, Holstein and F-1 strain Holstein Zebu) were grazing together on a meadow composed of Guinea grass (Panicum maximum), Brachiaria (Brachiaria decumbens) and a mixture of natural pastures, all in association with Leucaena (Leucaena leucocephala) sown at a density of 555 plants per ha in order to evaluate the potential of that system for beef production. A completely randomized design was used. The meadow was rotationally grazed at stocking rate of 3.6 animal per ha. During the 18 months of fattening the best results were obtained with the Zebu type. …

Changes In Understorey Pasture Composition In Agroforestry Regimes In New Zealand, M F. Hawke, A G. Gillingham

IGC Proceedings (1977-2023)

Long term Agroforestry trials were established in the North and South Islands of New Zealand between 1971-76. They compared a range of final tree stockings of Pinus radiata planted into pasture with open pasture control plots and were measured for tree growth parameters and agricultural production. This paper presents the results of pasture species changes over the period of tree age 10-22 years. Pasture species composition under Pinus radiata changed with time, dependent primarily on the rate of canopy closure. Changes occurred relatively rapidly in high tree stocked areas of 400 stems per hectare (sph) in the North Island trials …