Digital Commons Network^™

Copper Toxicity And Calcium Supplementation On Nkx2.5 And Mt2 Gene Expression In Zebrafish Embryos, Sahib Mohammad Bakir, Theodore B. Henry, Richard D. Handy

School of Biological and Marine Sciences

Background: Heavy metals are pervasive pollutants in aquatic ecosystems, posing significant threats to the survival and development of aquatic organisms, particularly during their early life stages. While copper is necessary for fish growth and maturation, elevated levels of dissolved copper in water can lead to adverse effects on early developmental stages. The aim of this study was to evaluate the expression pattern of the nkx2.5 gene and the potential of Mt2 expression as a biomarker for metal toxicity. Methods: The study assessed the expression levels of the nkx2.5 gene at different time points during embryonic development, focusing particularly on its …

Profiling Of Voltage-Gated Sodium Channel Subtype Expression And Resulting Susceptibility To Targeted Osmotic Lysis In Cancer, Spencer Gerard Lemoine

LSU Doctoral Dissertations

Cancer is a disease characterized by a wide array of dysregulated cellular function, activity, and growth. At times, specific dysregulations associated with cancer can be exploited as avenues of potential treatment. The sodium handling mechanisms and their associated proteins in cancer have received closer attention recently closely as such a potential mechanism to exploit for treating advanced cancer. Some novel treatments aim to inhibit the expression or activity of these sodium handling proteins in an attempt to prevent the cancer from advancing or becoming more aggressive; however, such approaches have had limited success. Targeted osmotic lysis (TOL) is a new …

Next Directions In The Neuroscience Of Cancers Arising Outside The Cns, Moran Amit, Corina Anastasaki, Robert Dantzer, Ihsan Ekin Demir, Benjamin Deneen, Karen O Dixon, Mikala Egeblad, Erin M Gibson, Shawn L Hervey-Jumper, Hubert Hondermarck, Claire Magnon, Michelle Monje, Shorook Na'ara, Yuan Pan, Elizabeth A Repasky, Nicole N Scheff, Erica K Sloan, Sebastien Talbot, Kevin J Tracey, Lloyd C Trotman, Manuel Valiente, Linda Van Aelst, Varun Venkataramani, Humsa S Venkatesh, Paola D Vermeer, Frank Winkler, Richard J Wong, David H Gutmann, Jeremy C Borniger

Faculty, Staff and Students Publications

The field of cancer neuroscience has begun to define the contributions of nerves to cancer initiation and progression; here, we highlight the future directions of basic and translational cancer neuroscience for malignancies arising outside of the central nervous system.

Bi-Allelic Acbd6 Variants Lead To A Neurodevelopmental Syndrome With Progressive And Complex Movement Disorders, Rauan Kaiyrzhanov, Aboulfazl Rad, Sheng-Jia Lin, Aida Bertoli-Avella, Wouter W Kallemeijn, Annie Godwin, Maha S Zaki, Kevin Huang, Tracy Lau, Cassidy Petree, Stephanie Efthymiou, Ehsan Ghayoor Karimiani, Maja Hempel, Elizabeth A Normand, Sabine Rudnik-Schöneborn, Ulrich A Schatz, Marc P Baggelaar, Muhammad Ilyas, Tipu Sultan, Javeria Raza Alvi, Manizha Ganieva, Ben Fowler, Ruxandra Aanicai, Gulsen Akay Tayfun, Abdulaziz Al Saman, Abdulrahman Alswaid, Nafise Amiri, Nilufar Asilova, Vorasuk Shotelersuk, Patra Yeetong, Matloob Azam, Meisam Babaei, Gholamreza Bahrami Monajemi, Pouria Mohammadi, Saeed Samie, Selina Husna Banu, Jorge Pinto Basto, Fanny Kortüm, Mislen Bauer, Peter Bauer, Christian Beetz, Masoud Garshasbi, Awatif Hameed Issa, Wafaa Eyaid, Hind Ahmed, Narges Hashemi, Kazem Hassanpour, Isabella Herman, Sherozjon Ibrohimov, Ban A Abdul-Majeed, Maria Imdad, Maksudjon Isrofilov, Qassem Kaiyal, Suliman Khan, Brian Kirmse, Janet Koster, Charles Marques Lourenço, Tadahiro Mitani, Oana Moldovan, David Murphy, Maryam Najafi, Davut Pehlivan, Maria Eugenia Rocha, Vincenzo Salpietro, Miriam Schmidts, Adel Shalata, Mohammad Mahroum, Jawabreh Kassem Talbeya, Robert W Taylor, Dayana Vazquez, Annalisa Vetro, Hans R Waterham, Mashaya Zaman, Tina A Schrader, Wendy K Chung, Renzo Guerrini, James R Lupski, Joseph Gleeson, Mohnish Suri, Yalda Jamshidi, Kailash P Bhatia, Barbara Vona, Michael Schrader, Mariasavina Severino, Matthew Guille, Edward W Tate, Gaurav K Varshney, Henry Houlden, Reza Maroofian

Faculty, Staff and Students Publications

The acyl-CoA-binding domain-containing protein 6 (ACBD6) is ubiquitously expressed, plays a role in the acylation of lipids and proteins and regulates the N-myristoylation of proteins via N-myristoyltransferase enzymes (NMTs). However, its precise function in cells is still unclear, as is the consequence of ACBD6 defects on human pathophysiology. Using exome sequencing and extensive international data sharing efforts, we identified 45 affected individuals from 28 unrelated families (consanguinity 93%) with bi-allelic pathogenic, predominantly loss-of-function (18/20) variants in ACBD6. We generated zebrafish and Xenopus tropicalis acbd6 knockouts by CRISPR/Cas9 and characterized the role of ACBD6 on protein N-myristoylation with myristic acid alkyne …

Induction Of Viral Mimicry Upon Loss Of Dhx9 And Adar1 In Breast Cancer Cells, Kyle A Cottrell, Sua Ryu, Jackson R Pierce, Luisangely Soto Torres, Holly E Bohlin, Angela M Schab, Jason D Weber

2020-Current year OA Pubs

UNLABELLED: Detection of viral double-stranded RNA (dsRNA) is an important component of innate immunity. However, many endogenous RNAs containing double-stranded regions can be misrecognized and activate innate immunity. The IFN-inducible ADAR1-p150 suppresses dsRNA sensing, an essential function for adenosine deaminase acting on RNA 1 (ADAR1) in many cancers, including breast. Although ADAR1-p150 has been well established in this role, the functions of the constitutively expressed ADAR1-p110 isoform are less understood. We used proximity labeling to identify putative ADAR1-p110-interacting proteins in breast cancer cell lines. Of the proteins identified, the RNA helicase DHX9 was of particular interest. Knockdown of DHX9 in …

Intestinal Cell Diversity And Treatment Responses In A Parasitic Nematode At Single Cell Resolution, Rahul Tyagi, Bruce A Rosa, Amanda Swain, Maxim N Artyomov, Douglas P Jasmer, Makedonka Mitreva

2020-Current year OA Pubs

BACKGROUND: Parasitic nematodes, significant pathogens for humans, animals, and plants, depend on diverse organ systems for intra-host survival. Understanding the cellular diversity and molecular variations underlying these functions holds promise for developing novel therapeutics, with specific emphasis on the neuromuscular system's functional diversity. The nematode intestine, crucial for anthelmintic therapies, exhibits diverse cellular phenotypes, and unraveling this diversity at the single-cell level is essential for advancing knowledge in anthelmintic research across various organ systems.

RESULTS: Here, using novel single-cell transcriptomics datasets, we delineate cellular diversity within the intestine of adult female Ascaris suum, a parasitic nematode species that infects animals …

Vista Checkpoint Inhibition By Ph-Selective Antibody Sns-101 With Optimized Safety And Pharmacokinetic Profiles Enhances Pd-1 Response, Thomas Thisted, Yoshiko Takeuchi, Robert D Schreiber, Et Al.

2020-Current year OA Pubs

VISTA, an inhibitory myeloid-T-cell checkpoint, holds promise as a target for cancer immunotherapy. However, its effective targeting has been impeded by issues such as rapid clearance and cytokine release syndrome observed with previous VISTA antibodies. Here we demonstrate that SNS-101, a newly developed pH-selective VISTA antibody, addresses these challenges. Structural and biochemical analyses confirmed the pH-selectivity and unique epitope targeted by SNS-101. These properties confer favorable pharmacokinetic and safety profiles on SNS-101. In syngeneic tumor models utilizing human VISTA knock-in mice, SNS-101 shows in vivo efficacy when combined with a PD-1 inhibitor, modulates cytokine and chemokine signaling, and alters the …

A Comparative Analysis Of Serpind1 In A Genomic Lens In Sharks, Vallabh Korivi, Samuel Angel

Undergraduate Student Symposium

This research investigates differences in wound healing between sharks and humans, focusing on key components in the healing pathway. Sharks are known for their remarkable lesion-healing capabilities. Genes such as SERPIND1 play crucial roles in tissue remodeling for sharks while playing a role in cancer metastasis and inflammation in humans. The SERPIND1 gene codes for plasminogen activator inhibitor 1 (PAI-1), which is involved in blood clotting. This clotting process is vital for repairing dermal wounds, sealing off the damaged area, and preventing further blood loss. Comparative genomic analysis reveals distinctions between human and shark SERPIND1 genes at different levels such …

A Comparison Of The Impact Of Colonial Heritage On The Development Of Former Western And Soviet Union Colonies, Sophia Wehle

Undergraduate Student Symposium

How successful were countries colonized by the West in developing compared to countries colonized by the Soviet Union. This paper presented three hypotheses: Former Soviet colonies will be more economically developed than former Western colonies, Former Soviet colonies will be more politically developed than former Western colonies, Former Soviet colonies will be more socially developed than former Western colonies. These hypotheses are derived from the theory that the types of colonialism that the Soviet Union and Western countries practiced were different, meaning that Western European nations created colonies to extract resources from them, rather than to form permanent bonds. This …

A Quantitative Analysis Of Sudoku Variant Puzzles, Nhat Yen Nhi Nguyen

Undergraduate Student Symposium

Sudoku is a globally popular number puzzle game whose appeal lies in its simplicity and capacity to challenge logical and analytical skills, transcending cultural barriers and finding applications in various fields. The game is believed to bring many benefits to the players, including stimulating the mind, as well as developing their ability to make decisions (Uniyal, 2022). This research delves into evolving preferences by analyzing publicly available data from Logic Masters Deutschland (LMD), a leading German organization dedicated to expanding Sudoku logic puzzles through competitions and events (www.logic-masters.de). The dataset comprises a wealth of puzzle-related information, including puzzle names, author …

A1 Vs A2 Milk Variants And Their Impact On Cysteine Metabolism In Autism-Spectrum Disorder, Esha Patel, Peter Kim

Undergraduate Student Symposium

Dairy cow milk stands as a vital source of nutrients encompassing proteins, carbohydrates, fats, and micronutrients. This nutritional complexity is heightened by the choice between A1 and A2 milk types. This research probes the distinctions between A1 and A2 milk and their potential implications for cysteine metabolism within the context of autism spectrum disorder (ASD). This study focuses on BCM7, a bioactive opioid peptide exclusively released during digestion of A1 milk, and its impact on mitochondrial respiration of macrophage-like THP1 cells.

Using the Seahorse XF for mitochondrial assessment, oxygen consumption rate (OCR), extracellular acidification rate (ECAR), basal respiration, proton leak, …

Alcohol Use Among High School Students In Select Florida School Districts, A Comparison Of Pre-Covid And Covid Years, (2019, 2021), Marlee Dauphin, Roohika Rastogi

Undergraduate Student Symposium

Background: The widespread concern about alcohol use among high school students underscores the urgent need for effective prevention and education programs. Comparing underage drinking statistics before and during the COVID-19 pandemic is essential for understanding patterns of behavior during this unique time period. This knowledge can potentially illuminate changes in risk factors and guide targeted interventions to protect the well-being of adolescents in the post-pandemic era.

Methods: The Youth Risk Behavior Survey (YRBS) data was used to analyze alcohol use behaviors in six Florida counties. We used student responses to examine alcohol-use initiation and whether they were currently drinking at …

Assessing Cardiologists' User Experience With Electronic Medical Records And Generative Artificial Intelligence Visualization, Colin Pulickathadam, David Giordano

Undergraduate Student Symposium

Cardiovascular Diseases (CVDs) are a leading cause of death worldwide, accounting for 17.3 million deaths per year. Artificial intelligence has gained prominence in recent years, and there is a significant interest in exploring how artificial intelligence can improve patient outcomes and decrease hospitalization rates. This study will identify the most critical values that cardiologists consider when evaluating patients’ charts, including factors such as medical history, diagnostic results, and current medications. Additionally, the study explores cardiologists' attitudes towards the integration of AI-generated imagery based on these critical values to enhance the efficiency and quality of patient care. A comprehensive survey will …

Assessing Sociodemographic Impact On Mental Health Experience In University Students, Kaitlin Bohorquez

Undergraduate Student Symposium

The COVID-19 pandemic helped shift mental health conversations from taboo to embraced among younger generations. With this shift, young people may be more willing to report on their mental health status, aiding professionals in understanding the relationship between mental health and overall health status. Research supports that factors vary among youth mental health experiences, but gaps in knowledge on the extent sociodemographic factors contribute still exists. This study investigates associations between the mental health experiences (MHE) and sociodemographic factors among college students. This knowledge can inform how to craft mental health programs to suit the needs of diverse university student …

Assessing The Impacts Of Social Media In Educating Individuals On The Importance Of Influenza Prevention, Alianys Rodriguez, Daniella Martinez

Undergraduate Student Symposium

Social media serves as a powerful tool for organizations to spread information to the public. This research, conducted in collaboration with Broward Regional Health Planning Council (BRHPC), a nonprofit organization that has an online presence on Facebook, Instagram, X, and LinkedIn, investigates the benefits of using social media as a platform to educate and raise awareness regarding the importance of influenza vaccines among individuals and communities. In the realm of public health, individuals must understand the importance of influenza vaccinations, as a way to prevent the spread of the seasonal flu and minimize its impacts on different communities. A total …

Assessment Of Brain Function And Soccer Penalty Kick Performance In Collegiate Soccer Players, Toniann Brezina

Undergraduate Student Symposium

BACKGROUND: The purpose of this research study is to assess National Collegiate Athletic Association (NCAA) men’s and women’s soccer players brain function while taking penalty kicks wearing an electroencephalogram (EEG) band and a heart rate monitor. An EEG is a device used to assess brain activity and will record four types of brain wave activities: alpha, beta, theta, and delta. Heart rate data provides additional insight during strenuous activity. Minimal research exists on EEG and soccer penalty kicks, but multiple published studies discuss high activity in the beta wave along with a low heart rate indicating a focused but relaxed …

Assessment Of Wearable Vert Technology In Collegeiate Volleyball Athletes, Nicole Stanchfield

Undergraduate Student Symposium

The purpose of this research study is to assess the use of the VERT device with (NCAA Division II Volleyball players. The VERT is a wearable and portable device that measures multiple variables including workload, jump height, frequency, intensity, and impact. Previous research indicates accurate data overall on jump performance which can then be applied to player work loads and injury risk. NCAA Division II Volleyball players included in this study will use the VERT device for one collegiate volleyball season, including practices and games. A typical season includes between 4-5 practices in a week and 1-3 games per week. …

Bees And Bvocs: Characterization Of Scents From Flowers And What Bees Leave Behind, Chase Pagley, Dana Kraska, Maria Castellon

Undergraduate Student Symposium

Insect pollinators, such as bees, are an essential component to their ecosystem and surrounding plant life. Flowers emit odors, also known as biological volatile organic compounds (BVOCs), to attract bees to themselves during pollination. Additionally, when bees visit flowers, they are known to leave “scent marks” to communicate with other hive members which flowers to select or avoid for pollination. While BVOCs have been identified for many flowering species, there has not been extensive work analyzing odors from flowers that are popular with bee pollinators, such as Bidens alba (Spanish Needle). Furthermore, studies have not explored which odors are scent …

Burst Size Determination Of A Newly Discovered Yersinia Pestis Bacteriophage, Jewel Mousa

Undergraduate Student Symposium

The escalating global resistance to antibiotics has called for innovative alternatives to combat bacterial infections. Bacteriophages, viruses that can specifically target and destroy antibiotic-resistant bacteria, have emerged as a promising potential solution in phage therapy. This research focuses on a newly discovered phage capable of infecting Yersinia pestis, the causative agent of the bubonic plague, through the lytic cycle. In the lytic cycle, bacteriophages infect and replicate within bacterial cells, causing the cells to burst and release newly formed phages. Specifically, this study aims to characterize this recently discovered phage, utilizing experiments to determine its burst size, which indicates …

Changes In The Nse And S100b Levels In Plasma Following Treatment With Peripheral Neuropathy-Inducing Chemo Drugs, Shriya Selvakumar, Priya Vontikommu

Undergraduate Student Symposium

Peripheral Neuropathy (PN) is a common chemotherapy-induced side effect that impacts 50% of all patients who receive chemotherapy. Treatment with Bortezomib (BTZ), Cisplatin (CIS), and Vincristine (VIN) has been established as the most common triggers of Chemotherapy-Induced Peripheral Neuropathy (CIPN) among cancer patients. Therefore, it was hypothesized that identifying diagnostic/prognostic biomarkers that can accurately predict the onset or progression of CIPN would help to determine the eligibility of the above-mentioned drugs for chemotherapy use and also would help to proactively prepare the patients for protective care. Therefore, our initial goal was to elucidate the molecular mechanisms underlying the onset and …

Characterization Of Exosomes From Melanoma Cells, Kishan Goli

Undergraduate Student Symposium

Melanoma is a specific type of skin cancer associated with the melanocytes developing into malignancy. Melanocytes produce a pigment that provides protection from the sun’s ultraviolet radiation. Melanocytes produce this pigment when ultraviolet radiation hits the skin, causing damage to the epidermis. When the epidermis is damaged, it triggers a signal to produce melanin to protect against ultraviolet radiation. However, too much exposure can cause DNA damage within the epidermis, especially within melanocytes, leading to the inhibition of regulated mitosis. Hence, non-regulated mitosis can lead to excessive melanocyte production, which may result in melanoma. Exosomes are vesicles generated by all …

Characterizing Visual Literacy In Biochemistry: Interpreting The Symbolic Language Embedded Within Biochemistry Representations, Sahil Sheth, Manav Patel

Undergraduate Student Symposium

Representations play a key role in communicating concepts and phenomena in courses such as chemistry, biology, and biochemistry. While representations have the potential to help students develop conceptual understanding of a subject, integrating multiple ideas to interpret and use the representations can be challenging. For example, biochemistry students are faced with the unique challenge of having to combine their knowledge of both chemistry and biology to make sense of complex biological structures and processes. Using the Visual Literacy Framework proposed by Schönborn and Anderson, the objective of our study is to investigate the symbolism embedded within biochemistry representations and how …

Christian Monasticism And The Making Of Modern Medicine In London, Nicole Shaker

Undergraduate Student Symposium

Christian monasteries were the first formal healthcare settings in London. They defined doctors as Jesus-imitators, hospitals as inclusive shelters, and medicine as the holistic treatment of mental, spiritual, and physical maladies. Today, London’s medical students are taught to strive towards Christian values like empathy and patience and London’s model of free healthcare represents inclusivity and generosity. But because medicine has evolved away from faith, London’s healthcare ultimately displays a watered-down image of its roots in love-based monastic service. This paper will examine how three values of monastic medicine in London during the Late Middle Ages— agape love, hospitality, and holism— …

Climate Change Attitudes About Human Responsibility Among The Nsu Community, Anshu Jasani

Undergraduate Student Symposium

This research is about how people self-report climate change attitudes about human responsibility. Self-reports precede actions (Critchfield & Perone, 1990), so a predictive model of attitudes is important. Ransdell (2016) surveyed a similar NSU sample in 2015 and found that 62% reported a belief that climate change is mostly human-caused. The present study is a replication with some additional questions. As before, all with NSU email addresses were sent the survey once, without any incentive or follow-up. In this survey, the main goal was to predict Question 6 which showed participants' views on climate change as mostly human caused. The …

Comparative Genomics Across Ganoderma Species To Identify Anti-Cancer Producing Secondary Metabolite Clusters, Abril Chao, Omobolanle Ayangade, Rajendra Pangeni Ph.D., Braham Dhillon Ph.D.

Undergraduate Student Symposium

Ganoderma lucidum is a mushroom-forming fungus that has been used in traditional Chinese medicine for over 2,000 years. G. lucidum, also known as lingzhi or reishi, is consumed in several Asian cultures to promote health and longevity. Bioactive molecules with anticancer properties have been isolated and characterized primarily from the species Ganoderma lucidum, and the cytotoxic potential of other species in the genus Ganoderma remains relatively unclear. Bioactive molecules isolated from mushroom fruiting bodies are demonstrated to have cytotoxicity against different cancer cell lines. Triterpenes and polysaccharides are the two major groups of compounds that exhibit antitumor and …

Comparing Methodologies In 2-2'-Bipyridine Synthesis, Valentina El Khouri Faieta, Emily Andersen, Cameron Martinez

Undergraduate Student Symposium

The purpose of this study is to determine the most economical and efficient way to synthesize functionalized bipyridine adducts for future incorporation into ruthenium complexes. These complexes show promise in treating bladder cancer and their extensive conjugated system demonstrates strong photovoltaic activity.

Two distinct approaches will be contrasted. The first method uses several disubstituted 2-bromopyridine substrates in homo-coupling reactions to create functionalized bipyridines, at the expense of long reactions times. The second route utilizes unsubstituted 2,2’-bipyridines and functionalizes them in a symmetrical pattern using a microwave synthesizer, to attempt to drastically shorten reaction times.

The first mode utilizes deactivating groups …

Comparing The Effects Of Invasive Waterhyacinth On Aquatic Communities, Maria José Gacharná Ferro, Colby Holbrook, Reginald Kempson Iii, Andrew Kuntz, Layla Lacelle, Claire Scobie, Indaya Byer

Undergraduate Student Symposium

Waterhyacinth is an invasive freshwater species that rapidly grows across water bodies, creating dense mats that disrupt local ecosystems. Our goal was to measure the impact of waterhyacinth on aquatic communities. We tested the hypotheses that waterhyacinth supported different fish and invertebrate species than native aquatic plants, used more dissolved oxygen (DO), and provided different levels of support to abundant invertebrates like amphipods. The fish communities under both plant groups were collected using a 1m2 throwtrap and a backpack electrofisher. This allowed for comparison between fish communities and method efficiency. We compared invertebrate community assemblages by collecting samples of roots …

Conserved Domains In Promoters Of Differentially Expressed Genes Under High Night Temperature Stress In Rice: How To Find Them And What Can They Tell Us?, Emalynn Tobias, Jenna Handal, Paul Lup, Michael Egozi

Undergraduate Student Symposium

Crop plant response to stress involves changes in gene expression patterns. The complex process of regulation of gene expression involves cis- and trans-acting components. One of the key steps in understanding changes in gene expression associated with a plant’s response to stress begins with identifying “conserved domains” in the promoters of differentially expressed genes (DEGs). By providing binding sites for transcription factors, the conserved domains can play a crucial role in gene regulation. In this study, we aim to identify the cis-regulatory elements (CREs) in the promoters of 149 DEGs that were identified in the transcriptomic analysis of two rice …

Determining The Function Of The Protein Of Interest 2qru Using Bioinformatic Tools And Wet-Lab Experiments, Samhita Doranala, Michelle Milman, Marissa Bonsangue, Eliezer Gurovich

Undergraduate Student Symposium

Proteins are complex macromolecules that play a vital role in the facilitation of biological processes. Protein research and documentation are crucial for future advancements. They aid in developing medications, disease diagnosis and treatment, and the understanding of structural biology. Thousands of proteins have been stored in the Protein Data Bank (PDB) since 1971. Although their structures are known, many of their functions have yet to be determined.

This study was conducted in order to shed light on the biological role of a previously uncharacterized protein of interest (POI) with the PDB ID: 2QRU. A two-pronged approach involving both bioinformatics and …

Determining The Optimal Infection Temperature And Burst Size Of Gordonia Rubripertincta Bacteriophage, Genesis Aguilar, Bianca A. Gonzalez, Sarah Moya, Jose C. Padron, Dani Sheil

Undergraduate Student Symposium

Antibiotic resistance represents a significant global health crisis, especially within hospitals, creating a necessity for the search for alternative therapeutic agents to combat drug-resistant pathogens. One source of treatment is phages specifically targeting G. rubripertincta, a gram-positive, aerobic actinomycete bacterium which has shown intrinsic resistance to some antibiotics. Furthermore, G. rubripertincta can cause endocarditis and CNS infections in immunocompromised individuals. Several phages such as Alyssamiracle, Fribs8, Genamy16, and NovaSharks, have been discovered that successfully target G. rubripertincta. The goal of this research is to determine the optimal infection temperature and the burst size of these phases. By growing …