Digital Commons Network^™

Annotation Of Three Actinobacteriophages: Tuktuk, Shamu, And Megatron06, Lauren Fisk, Kylie Foss, Khloe Hester, Yuki Kobayashi, Madelin Kvatek, Elsie Larson, Joshua Lewis, Madilyn Manary, Laura Nelsen, Thomas Ostrem, Cayli Penner, Sydney Rehmke, Taylor Rentschler, Adriana Sandbulte, Dane Shoenborn, Jonas Tiensvold, Shayler Van Gelder, Kallie Van Kley, Megan Vangarven, Brianna Winford, Byron Noordewier, Sara S. Tolsma

Celebration of Research

We annotated three newly discovered bacteriophages. TukTuk and Shamu were isolated on the host Microbacterium folorium and Megatron06 on Mycobacterium smegmatis. Based on based on gene-content similarity (GCS) of 35% or higher to sequenced bacteriophages present in the Actinobacteriophage database, phagesDB, TukTuk was assigned to cluster EB, Shamu to cluster EA, and Megatron06 to Cluster H1. Here we report a summary of our annotation findings along with one in-depth analysis of an aspect of our annotation for each phage.

The Gut Microbiome In Chronic Diseases, Emily Mcdonald, Katlyn Bombei, Madelyn Weber

Celebration of Research

Cardiovascular disease, chronic kidney disease, diabetes, and various mental health conditions have been shown to be associated with gut microbiota alterations that may contribute to the development and pathogenesis of those diseases. Cardiovascular disease, inflammatory bowel disease and diabetes have been associated with a lack of butyrate- producing bacteria which are responsible for lowering blood pressure, limiting atherosclerosis, abdominal pain, and lowering insulin resistance. Medications for individuals with schizophrenia may decrease bacteria with anti-inflammatory properties. Potential research avenues may be directed at investigating the di@erences between gut microbiomes between men and women to cultivate unique and targeted treatment and prevention …

Mousing Around With Bacteriophages: ​Using Antibodies Raised In Balb/C Mice To Analyze Phages​, Grace Anderson, Jewel Bergstrom, Samuel Brandt, Maria Garcia, Sadie Gilmeister, Aimee Hulstein, Erika Mckenney, Tyler Nhan, Cayli Penner, Taylor M. Rentschler, Jessica Slight, Anneka Sterk, Jacie Vanderwaal, Elliana Zerr, Sara S. Tolsma

Celebration of Research

Bacteriophages are viruses that infect bacteria and can be used in phage therapy to combat bacterial infections resistant to traditional antibiotics. One current limitation of phage therapy is the lack of understanding about phage genetics and biochemistry. We can learn more about bacteriophages by identifying patterns in their protein structure. In our experiments, we immunized BALB/c mice using whole phage or ~71>kDa proteins extracted from Sbash and Island3. We tail-bled the mice to collect antibodies raised against these phages, then used ELISA assays to detect anti-phage protein antibodies in mouse serum. We are using our antibodies and Western blot …

Interventions To Reduce Risk Of Perineal Tearing, Bailey Varpness, Kylie Lyman, Sandra Muyskens

Celebration of Research

Perineal tearing is one of the most common injuries that can be sustained during vaginal delivery, it can be physically and mentally debilitating for the mother to endure. Preventing this injury can lead to an easier healing process for the mother, allowing her to have a more impactful postpartum journey. There is debate as to whether warm compresses, perineal massage, and alternative birthing positions decrease the incidence of perineal tearing. This review of literature focuses on how these interventions work and if they are effective at decreasing the risk for perineal tearing during vaginal delivery.

Discovering Gene Functions In Mycobacteriophage Sbash Using A Genetic Screen, Erin Brouwer, Daiki Jonouchi, Byron Noordewier, Sara S. Tolsma

Celebration of Research

Sbash is a temperate bacteriophage, which was isolated on the host, Mycobacterium smegmatis mc2 155 from soil collected in South Africa. It is classified as cluster I and sub-cluster I2. Its genome consists of 55,832 base pairs and 89 protein-coding genes of which only 25 genes were assigned a function by bioinformatic analysis. We are using a genetic screen to uncover the functions of phage genes for which function is unknown. To begin to uncover the functions of the protein products of the genes in Sbash’s genome, we cloned each gene into the pExTra plasmid and assayed each phage gene …

Comparisons Of Spiritual Well-Being, Psychological Well-Being, And Suicidal Ideation Between Usa And Taiwanese College Students, Pi-Ming Yeh Associate Professor, Cheng-Huei Chiao Professor, Jennchang Liou Professor

Epsilon Sigma at-Large Research Conference

Background: In Taiwan, there were 7,038 reports of attempted suicide among people aged 24 or younger in 2018. Anxiety disorders are the most common mental illness in the U.S., affecting 40 million adults in the United States age 18 and older.

Purpose: In this study, we compared United States (US) and Taiwanese college students’ spiritual well-being, psychological well-being, and suicidal ideation.

Methods: A total of 661 college students (481 from the US and 180 from Taiwan) were recruited to complete the survey study. This was a cross-sectional comparative research design. SPSS 28.0 was used to do the data analysis. Chi-square …

Tension Activation Of Mechanosensitive Two-Pore Domain K+ Channels Traak, Trek-1, And Trek-2., Ben Sorum, Trevor Docter, Vincent Panico, Robert A Rietmeijer, Stephen G Brohawn

Cooper Medical School of Rowan University Departmental Research

TRAAK, TREK-1, and TREK-2 are mechanosensitive two-pore domain K+ (K2P) channels that contribute to action potential propagation, sensory transduction, and muscle contraction. While structural and functional studies have led to models that explain their mechanosensitivity, we lack a quantitative understanding of channel activation by membrane tension. Here, we define the tension response of mechanosensitive K2Ps using patch-clamp recording and imaging. All are low-threshold mechanosensitive channels (T10%/50% 0.6-2.7 / 4.4-6.4 mN/m) with distinct response profiles. TRAAK is most sensitive, TREK-1 intermediate, and TREK-2 least sensitive. TRAAK and TREK-1 are activated broadly over a range encompassing nearly all physiologically relevant …

Poster Abstracts For 14th Annual University Of Dayton And Miami Valley Hospital Healthcare Symposium, Udayton

Pre-Health Resource Center Campus Communications

No abstract provided.

Spsc Minutes 2024-04-11, Northern Illinois University

SPS Council Archives

No abstract provided.

Individual Differences In Cannabis Use Disorder With Implications For Endocannabinoid Modulation In Therapeutics Development, Erin L. Martin

MUSC Theses and Dissertations

Cannabis use disorder (CUD) is increasingly prevalent in the United States, but there is no effective pharmacological means to treat it. The endocannabinoid (eCB) system has emerged as a candidate therapeutic target demonstrating some evidence of efficacy in treating CUD. However, clinical trials evaluating eCB-modulating therapeutics have historically undervalued individual differences that could contribute to variation in treatment outcome (e.g. sex, comorbid psychiatric illness). To address this gap in the literature, the present set of studies (a) compared plasma eCB tone in groups underrepresented in treatment trials for CUD (females, individuals with comorbid major depressive disorder; MDD/CUD) with males or …

Scholastic Minutes 04/11/2024, Scholastic Committee

Scholastic Committee

No abstract provided.

The Comment, April 11, 2024, Bridgewater State University

The Comment

No abstract provided.

Patterns, Drivers, And Implications Of Postfire Delayed Tree Mortality In Temperate Conifer Forests Of The Western United States, Sebastian Busby, Cody Evers, Andrés Holz

Geography Faculty Publications and Presentations

Conifer forest resilience may be threatened by increasing wildfire activity and compound disturbances in western North America. Fire refugia enhance forest resilience, yet may decline over time due to delayed mortality—a process that remains poorly understood at landscape and regional scales. To address this uncertainty, we used high-resolution satellite imagery (5-m pixel) to map and quantify delayed mortality of conifer tree cover between 1 and 5 years postfire, across 30 large wildfires that burned within three montane ecoregions in the western United States. We used statistical models to explore the influence of burn severity, topography, soils, and climate moisture deficit …

Spectator 2024-04-11, Editors Of The Spectator

The Spectator

No abstract provided.

Sexual Assault And The Impact On Identity And Mothering: Women's Lived Experiences, Madelin Cerullo

Counseling and Psychology Dissertations

Literature on traumatic events, particularly those of a sexual nature, suggests that these events have an impact on one’s wellbeing and ability to parent. However, limited literature centers a women’s experience, with little attention focused on identity formation, the role of mothering, and healing as an outcome (Babcock et al., 2015; Clifford et al., 2020. Much extant literature focuses on predetermined themes that narrow participants’ reports and fail to capture lived experiences (Carothers Bert et al., 2009) with emphasis on significant disruptions to lives because of the traumatic event (Clifford et al., 2020). When literature considers these topics as part …

Far Out: The Extended Denial Of Public Access To Psychedelic Therapeutics, Andrew R. Waldeck

Texas A&M Journal of Property Law

The United States patent regime is designed to promote dissemination of information that undergirds a particular innovation. To incentivize disclosure, inventors are granted a time-limited right to exclude others from practicing the invention, thereby affording the inventor a period in which to commercialize and financially benefit from their inventive contribution. The disclosure provides information sufficient for one of skill in the relevant art to make and use the invention, and the public may freely do so upon the patent’s expiry. Global advancement of human medicine is fundamentally intertwined with the United States patent system; medical progress largely depends upon the …

Enhancement Of Applied Rice Breeding Programs: Exploring Factors Impacting Genomic Selection Accuracy And Discovery Of A Novel Gene For Grain Shape, Maria G. Montiel

LSU Doctoral Dissertations

Plant breeding is a crucial tool in addressing global challenges such as climate change, population growth, and the need for more resilient crops. Rice serves as a staple food for a significant portion of the global population and has served as a model crop for genetic and genomic studies. Genomic selection (GS) has become valuable tool in plant breeding, allowing breeders to predict breeding lines’ performance based on their genotypes. The training (TS) set is a central component of genomic selection and the optimization of the training set is crucial for efficient implementation of genomic selection. The first two objectives …

Research On 3d Printing Resin Exposure Properties And Its Application On Centrifugal Microfluidic Platform Based On Fluorescence Detection, Zheng Qiao

LSU Doctoral Dissertations

This dissertation encapsulates significant advancements in the field of SLA 3D printing and centrifugal microfluidics. Central to the research is the development of a novel mathematical model for predicting trapped resin thickness in SLA 3D printing, a groundbreaking contribution that addresses a critical aspect of printing intricate structures. This model, the first to establish a mathematical relationship for resin thickness, is rooted in a comprehensive study of the resin curing process. The research leverages the concept of 'critical dosage' for resin curing, leading to a more refined and theoretically grounded approach for calculating curing thickness. Experimentation further validates the model, …

2024 Undergraduate Research Symposium Program, Coastal Carolina University

Undergraduate Research Symposium Programs

15th Annual Undergraduate Research Symposium, April 10-11, 2024.

Engineering Mesenchymal Stromal Cells To Suppress Inflammation And Increase Beta-Cell Mass For Treating Type 1 Diabetes, Dhruva Patel

MUSC Theses and Dissertations

Type 1 diabetes (T1D) is a life-threatening autoimmune disease where the immune system destroys the insulin producing beta-cells in the pancreas. There is no cure for the disease, and the current treatment involves life-long, daily injection of insulin. An ideal therapy for T1D will be the suppression of autoimmunity and increased functional beta-cell mass. The immune regulatory cytokine IL10 can suppress inflammation in various autoimmune diseases. On the other hand, several growth factors and peptide hormones are known to promote an increase in beta-cell mass, through neogenesis and beta-cell proliferation. However, efficient induction of increased beta-cell mass requires preferential and …

The Current - Volume 34 Issue 12, Nova Southeastern University

Student Newspapers

No abstract provided.

Examining Adverse Childhood Experiences And Black Youth's Engagement In A Hospital-Based Violence Intervention Program Using Administrative Data, Laura A. Voith, Amy Korsh-Williams

Faculty Scholarship

Background: Stemming from poverty and systemic racism, Black youth are disproportionately represented in hospital-based violence intervention programs (HVIPs) due to greater violence exposure. HVIPs are a critical intervention that have been shown to reduce rates of reinjury in urban hospitals and trauma centers across the United States; however, they are plagued by low enrollment and engagement rates. Few studies have examined factors related to engagement, particularly among Black youth. Methods: Guided by Trauma Theory and Critical Race Theory, this study uses a retrospective cohort design. Between-group differences of adverse childhood experiences (ACEs) among engaged youth compared to nonengaged youth who …

Enhancing Nsclc Recurrence Prediction With Pet/Ct Habitat Imaging, Ctdna, And Integrative Radiogenomics-Blood Insights, Sheeba J Sujit, Muhammad Aminu, Tatiana V Karpinets, Pingjun Chen, Maliazurina B Saad, Morteza Salehjahromi, John D Boom, Mohamed Qayati, James M George, Haley Allen, Mara B Antonoff, Lingzhi Hong, Xin Hu, Simon Heeke, Hai T Tran, Xiuning Le, Yasir Y Elamin, Mehmet Altan, Natalie I Vokes, Ajay Sheshadri, Julie Lin, Jianhua Zhang, Yang Lu, Carmen Behrens, Myrna C B Godoy, Carol C Wu, Joe Y Chang, Caroline Chung, David A Jaffray, Ignacio I Wistuba, J Jack Lee, Ara A Vaporciyan, Don L Gibbons, John Heymach, Jianjun Zhang, Tina Cascone, Jia Wu

Faculty, Staff and Student Publications

While we recognize the prognostic importance of clinicopathological measures and circulating tumor DNA (ctDNA), the independent contribution of quantitative image markers to prognosis in non-small cell lung cancer (NSCLC) remains underexplored. In our multi-institutional study of 394 NSCLC patients, we utilize pre-treatment computed tomography (CT) and 18F-fluorodeoxyglucose positron emission tomography (FDG-PET) to establish a habitat imaging framework for assessing regional heterogeneity within individual tumors. This framework identifies three PET/CT subtypes, which maintain prognostic value after adjusting for clinicopathologic risk factors including tumor volume. Additionally, these subtypes complement ctDNA in predicting disease recurrence. Radiogenomics analysis unveil the molecular underpinnings of these …

Virtual Gaming, Actual Damage: Video Game Design That Intentionally And Successfully Addicts Users Constitutes Civil Battery, Allison Caffarone

Duke Law & Technology Review

In recent years, there has been increased academic interest in both the neurological effects of compulsive gaming and the potential tort liability of game developers who scientifically engineer games in order to addict users. Scholars from various disciplines are currently debating the scope and potential solutions to the problems associated with Gaming Disorder, now a globally recognized illness. This article contributes to this discussion by offering a multidisciplinary analysis of the scope of video game addiction, its neurological bases, and its relation to the legal rights and responsibilities of victims and game developers. In addition, this article explores the practical …

Shared Genomic Segments Analysis Identifies Mhc Class I And Class Iii Molecules As Genetic Risk Factors For Juvenile Idiopathic Arthritis, Cecile N. Avery, Nicole D. Russell, Cody J. Steely, Aimee O. Hersh, John F. Bohnsack, Sampath Prahalad, Lynn B. Jorde

Markey Cancer Center Faculty Publications

Juvenile idiopathic arthritis (JIA) is a complex rheumatic disease encompassing several clinically defined subtypes of varying severity. The etiology of JIA remains largely unknown, but genome-wide association studies (GWASs) have identified up to 22 genes associated with JIA susceptibility, including a well-established association with HLA-DRB1. Continued investigation of heritable risk factors has been hindered by disease heterogeneity and low disease prevalence. In this study, we utilized shared genomic segments (SGS) analysis on whole-genome sequencing of 40 cases from 12 multi-generational pedigrees significantly enriched for JIA. Subsets of cases are connected by a common ancestor in large extended pedigrees, increasing the …

The Holobiont, Food Justice, And Gaia 2.0 A Post-Human(Ist) Approach To Functional Medicine, Rosalynn A. Vega

Anthropology Faculty Publications

Functional medicine is a personalized and holistic approach to treating chronic disease. In this article, I build upon posthumanist literature by examing how functional medicine practitioners are decentering and destabilizing what it means to be human. Functional medicine discourse on the holobiont, which considers the human as an assemblage of different microbial species, reframes the “humananimal” (see Nayar 2018) as the “humicrobe.” I engage Gaia 2.0 (see Lenton and Latour 2018) when analyzing the interconnectivity, interdependence, and mutualism of all life. My approach to interconnectivity interweaves both functional medicine descriptions of systems biology and Luhmann’s (2012) approach to system’s theory …

April 11, 2024, The Daily Mississippian

Daily Mississippian (all digitized issues)

No abstract provided.

The World Health Organization Was Born As A Normative Agency: Seventy-Five Years Of Global Health Law Under Who Governance, Lawrence O. Gostin, Benjamin Mason Meier, Safura Abdool Karim, Judith Bueno De Mesquita, Gian Luca Burci, Danwood Chirwa, Alexandra Finch, Eric A. Friedman, Roojin Habibi, Sam F. Halabi, Tsung-Ling Lee, Brigit Toebes, Pedro Villarreal

Georgetown Law Faculty Publications and Other Works

The World Health Organization (WHO) was born as a normative agency and has looked to global health law to structure collective action to realize global health with justice. Framed by its constitutional authority to act as the directing and coordinating authority on international health, WHO has long been seen as the central actor in the development and implementation of global health law. However, WHO has faced challenges in advancing law to prevent disease and promote health over the past 75 years, with global health law constrained by new health actors, shifting normative frameworks, and soft law diplomacy. These challenges were …

Charting The Cellular Biogeography In Colitis Reveals Fibroblast Trajectories And Coordinated Spatial Remodeling, Paolo Cadinu, Kisha N Sivanathan, Aditya Misra, Rosalind J Xu, Davide Mangani, Evan Yang, Joseph M Rone, Katherine Tooley, Yoon-Chul Kye, Lloyd Bod, Ludwig Geistlinger, Tyrone Lee, Randall T Mertens, Noriaki Ono, Gang Wang, Liliana Sanmarco, Francisco J Quintana, Ana C Anderson, Vijay K Kuchroo, Jeffrey R Moffitt, Roni Nowarski

Faculty, Staff and Student Publications

Gut inflammation involves contributions from immune and non-immune cells, whose interactions are shaped by the spatial organization of the healthy gut and its remodeling during inflammation. The crosstalk between fibroblasts and immune cells is an important axis in this process, but our understanding has been challenged by incomplete cell-type definition and biogeography. To address this challenge, we used multiplexed error-robust fluorescence in situ hybridization (MERFISH) to profile the expression of 940 genes in 1.35 million cells imaged across the onset and recovery from a mouse colitis model. We identified diverse cell populations, charted their spatial organization, and revealed their polarization …

Seminoles, Soldiers, And Settlers: Identity And Power On The Florida Frontier, Jean Louise Lammie

USF Tampa Graduate Theses and Dissertations

This project uses an historical archaeological approach to analyze the processes of cultural negotiation between disparate social groups living in a remote borderland in the early to mid-nineteenth centuries. Florida, first colonized by the Spanish in the 1500s was passed to the United States in 1821 as part of the young nation’s nascent Manifest Destiny. The Seminoles experienced multiple colonial conflicts, dealt with new diseases, and selected aspects of European culture to incorporate into their daily practices of lifeways.

Archaeological studies of culture change tend to focus on the product of colonial interactions rather than the processes of change that …