Digital Commons Network^™

Genetically Modified Organisms: Progress Or Blight?, Martin Lopez

Capstone Projects and Master's Theses

This project explores the origins of genetically modified organisms otherwise known as GMOs. This project also seeks to provide in detail the harmful effects GMO crops could potentially have on human beings and the environment's biodiversity. It is with hope that this project is able to educate and encourage the public on other alternatives for food sources such as, farmers' markets, plant breeding, and at home gardening.

Benefits Of Environmental Education For Low-Income Elementary School Students, Victoria Denoyer

Capstone Projects and Master's Theses

This study analyzes the benefits of environmental education for low-income elementary school students. As the world is seeing a turbulent downfall in the state of the environment, fostering a healthy and eager generation to find solutions to this crisis is essential. Through analysis using a literature review and qualitative interviews and surveys with in-service teachers and outreach educators, the results determine there are benefits to implementing environmental education for low-income elementary school students. These benefits extend beyond environmental agency and profoundly impact the cognitive, mental, and physical development of low-income students.

A Cultural Communication Perspective On Hispanic Students’ College Experiences, Yanrong (Yvonne) Chang

Communication Faculty Publications

Guided by the theoretical assumption that college life is a culturally situated, symbolic process of interaction between students and various others, this qualitative study attempts to explore how minority students’ home culture, which is different from the dominant college culture, helps them make sense of their college experiences. The respondent interviewing method was used to obtain Hispanic students’ responses to questions that allow them to share their experiences, which were centered around student-professor interactions. A total of 112 Hispanic college students from a comprehensive university in South Texas participated in the study. Results confirmed the significance of examining minority students’ …

Assessing Health Implications Of Monarch Butterfly Populations And Behaviors, Kira Goldman, Kelsey E. Fisher Dr.

Honors Scholar Theses

Pollinator populations have seen massive declines over the past decade due to anthropogenic activity and climate change posing serious threats to the health of humans and ecosystems (Ulyshen & Horn, 2023). As agents responsible for the production of about 35% of micronutrient rich food crops necessary for adequate nutrition to lead healthful lives and prevent nutrition associated diseases, pollinator decline is a public health concern (Aizen et al., 2009). Monarch butterflies (Danaus plexippus) serve as bio indicators for the health of pollinator populations because of their analogous habitat and food resources shared with many other pollinator species, including bees and …

Understanding The Role Of Minor Intron Splicing In Spermatogenesis, Jade Rosado, Kazumasa Takemoto, Rahul Kanadia

Honors Scholar Theses

The minor spliceosome is composed of the unique small nuclear RNAs (snRNA), U11, U12, U4atac, and U6atac, and is necessary for the splicing of less than 0.5% of introns, termed minor introns. Minor intron containing genes (MIGs) regulate diverse processes, one of which is spermatogenesis. Specifically, 111 testis-specific MIGs have been identified, suggesting that minor splicing is necessary for spermatogenesis. To interrogate the role of minor splicing in spermatogenesis, we conditionally ablated the Rnu11 gene, which encodes for the U11 snRNA, in the developing testes via Stra8-iCre, creating a Rnu11^Flx/Flx::Stra8-Cre⁺ mutant. We found, at postnatal …

Analysis Of Antibiotic Sensitivity Patterns In Pasteurellaceae Family Isolates: A Microbial And Molecular Investigation, Jillian Barron

Honors Scholar Theses

Bacteria in the Pasteurellaceae family are known to cause disease in both human and animal species. Like all species of bacteria, the pathogens in the Pasteurellaceae family evolve and acquire antimicrobial-resistant traits. Antimicrobial resistance of bacteria is a significant public health concern, as treatment of resistant pathogens becomes more difficult, if not impossible, with current medical capabilities. In this project, phenotypic and genotypic analyses were performed on Pasteurellaceae family isolates sourced from the Connecticut Veterinary Medical Diagnostic Laboratory. Phenotypic antibiotic sensitivity patterns of each isolate were assessed against a panel of antibiotics through a Kirby-Bauer disk diffusion test. Disk diffusion …

The Association Between Aerobic Exercise And Changes In Hdl Quality Induced By Egg Consumption – A Post-Hoc Analysis Of A Randomized, Controlled, Crossover Dietary Intervention Study, Rachael Woodruff, Catherine J. Andersen

Honors Scholar Theses

Abstract

Objective: Daily intake of whole eggs and aerobic exercise have independently been shown to increase high-density lipoprotein cholesterol (HDL-C) and measures of HDL function, suggesting cardioprotective effects of these lifestyle interventions. However, it is unknown whether exercise volume can synergistically or differentially impact diet responses and egg effects on HDL. Therefore, we evaluated whether exercise volume was associated with distinct effects of consuming different egg-based diets on measures of HDL size, function, and composition.

Methods: Twenty-six healthy men and women completed a 16-week randomized, controlled, crossover intervention study which aimed to investigate the effects of egg-free, egg-white, and whole-egg …

Archaeal Diversity In The Anna's Hummingbird Microbiome, Lauren E. Chance

Honors Scholar Theses

The microbial communities that are present in and on vertebrates are collectively called the microbiome. The composition of a microbiome is dependent upon the host, the environment, and evolution. There has been extensive research on the bacterial composition of host-associated microbiomes, however, there has been much less work on the archaeal composition of host-associated microbiomes. Archaea have previously been assumed to primarily exist in extreme environments, but this may not be true and has been influenced by their generally low abundance and methodological difficulties in detection. It is possible they are consistent members of diverse host-associated microbiomes.

Archaea-specific PCR primers …

Investigating Effects Of Co-Culturing With Vibrio Fischeri On Secondary Metabolite Production Of Hawaiian Bobtail Squid Symbionts, Mariam A. Zedan

Honors Scholar Theses

Microbes are a fruitful source of natural product drug discovery since many of their secondary metabolites show defensive activity and have unique structures. However, extracting and elucidating the structure of those compounds can be challenging in vitro due to particular environmental and nutritional requirements of some bacteria. Those requirements may include proximity to other organisms that the microbes have symbiotic relationships with. Under those conditions, the organism may activate silent biosynthetic gene clusters (BGCs) responsible for production of certain secondary metabolites not typically expressed otherwise. One method of BGC induction is co-culturing with other microbes since this mimics the natural …

My Daily Grounds: A Journal Of Creative Alchemy, Kathleen A. Donovan

Creativity and Change Leadership Graduate Student Master's Projects

My Daily Grounds: A Journal of Creative Alchemy is a project intended to renew artistic confidence and prepare a mindset for a writing retreat in Ireland in June 2024 by revisiting childhood memories and exploring the impact of early domestic abuse through a visual journaling process. This endeavor aims to stir up the creative voice and find authenticity in creation by gathering and clarifying personal data visually, following the Creative (CPS) approach to unlock new insights and ideas for writing. The resulting essays will illuminate the unseen effects of trauma and are intended for publication, reflecting a co-creative process between …

Mutational Landscape Of Noncoding Ultraconserved Elements In Human Cancers: Functional Relevance And Clinical Utility, Recep Bayraktar

Dissertations and Theses (Open Access)

The mutational landscape of phylogenetically ultraconserved elements (UCEs), especially those in noncoding DNA regions (ncUCEs), and their functional and clinical relevance in cancers remain poorly characterized. In this thesis, we perform a systematic analysis of whole-genome sequencing (WGS) data from the International Cancer Genome Consortium (ICGC) and in-house targeted sequencing of 13,736 UCEs and demonstrate that ncUCE somatic alterations are pervasive. Using a genome-wide multiplexed CRISPR knockout screen in colorectal cancer (CRC) cell lines, we show that the loss of several mutated ncUCEs impacts cancer cell proliferation. In-depth functional studies in vitro and in vivo further reveal that UCE_11311 is …

Factors That Contribute To Challenging Behaviors With Early Childhood Students, Jennifer N. Robinson

Electronic Theses and Dissertations

There has been an increasing number of students being referred to mental health services due to behavioral problems they exhibit at school. Behavioral and social concerns impede academic success for the individual child as well as their classroom peers. Many factors can contribute to challenging behaviors in early childhood students within rural and high-poverty communities, an elementary school in the South-Atlantic region of the United States is experiencing some of those factors. The purpose of this qualitative research study is to investigate some of the possible factors which contribute to challenging behaviors and how those factors impact academic achievement. The …

Coastal-Ocean And Estuarine Acidification In Long Bay, South Carolina: A Comparison Of In-Situ Water Quality Monitoring With Measured Carbonate System Parameters, Mary Olsen

Electronic Theses and Dissertations

One of the major water-quality issues impacting our coast and estuaries is coastal-ocean and estuarine acidification. Due to a relative dearth of data in the southeastern United States it is increasingly difficult to determine the full extent and intensity of this problem. This study provides the first characterization of coastal-ocean and estuarine acidification in Long Bay, SC by attempting to leverage long-term water quality data sets at two coastal-ocean pier sites using in-situ YSI EXO sondes deployed at both the surface and bottom waters along with two estuarine sites sampled by volunteer monitors using Orion star multi-meters. Discrete samples (120) …

The Influence Of Researcher Disturbance On Raccoon Predation Of American Alligator Nests, Clarissa Tuten

Electronic Theses and Dissertations

Raccoons (Procyon lotor) are a dominant predator of American alligator (Alligator mississippiensis) nests in the southeastern United States, using a combination of olfactory, visual, and tactile cues to identify nest locations. Studies on alligator nesting ecology typically require researchers to create paths through marsh habitat, potentially introducing visual and olfactory cues raccoons may use to locate nests. The purpose of this study was to evaluate the effect of human visitation to alligator nests on the frequency of raccoon nest predation at two sites in coastal South Carolina, Tom Yawkey Wildlife Center (TYWC) and Santee Coastal Reserve (SCR). We hypothesized that …

Cooperative And Bifunctional Adsorbent-Catalyst Materials For In-Situ Vocs Capture-Conversion, Sukanta K. Mondal, Peter Aina, Ali A. Rownaghi, Fateme Rezaei

Chemical and Biochemical Engineering Faculty Research & Creative Works

Volatile Organic Compounds (VOCs) Are Gases that Are Emitted into the Air from Products or Processes and Are Major Components of Air Pollution that Significantly Deteriorate Air Quality and Seriously Affect Human Health. Different Types of Metals, Metal Oxides, Mixed-Metal Oxides, Polymers, Activated Carbons, Zeolites, Metal-Organic Frameworks (MOFs) and Mixed-Matrixed Materials Have Been Developed and Used as Adsorbent or Catalyst for Diversified VOCs Detection, Removal, and Destruction. in This Comprehensive Review, We First Discuss the General Classification of VOCs Removal Materials and Processes and Outline the Historical Development of Bifunctional and Cooperative Adsorbent-Catalyst Materials for the Removal of VOCs from …

Cross-Pollination: Building A Co-Taught Course To Examine Art And Sex Through The Lens Of Botany, Christopher T. Martine, Diamanda A. Zizis, Anna K. Kell

Faculty Journal Articles

Driven by overlapping interests in plants, art, and diversity in sex expression, Anna Kell (Department of Art and Art History) and Chris Martine (Department of Biology) developed a course that integrates the perspectives of a visual artist and a botanist. Art & Sex Through the Lens of Botany seeks to impart the importance of making connections across disciplines and the value of visual literacy across academic lines. The course introduces foundational concepts in each field and encourages students to integrate and explore these different systems of knowledge and their intersections. In addition to developing fluencies related to both general botany …

Tripeptide-Assisted Gold Nanocluster Formation For Fe3+ And Cu2+ Sensing, Jonghae Youn, Peiyuan Kang, Justin Crowe, Caleb Thornsbury, Peter Kim, Zhenpeng Qin, Jiyong Lee

Chemistry Faculty Publications and Presentations

Fluorescent gold nanoclusters (AuNCs) have shown promise as metal ion sensors. Further research into surface ligands is crucial for developing sensors that are both selective and sensitive. Here, we designed simple tripeptides to form fluorescent AuNCs, capitalizing on tyrosine’s reduction capability under alkaline conditions. We investigated tyrosine’s role in both forming AuNCs and sensing metal ions. Two tripeptides, tyrosine–cysteine–tyrosine (YCY) and serine–cysteine–tyrosine (SCY), were used to form AuNCs. YCY peptides produced AuNCs with blue and red fluorescence, while SCY peptides produced blue-emitting AuNCs. The blue fluorescence of YCY- and SCY-AuNCs was selectively quenched by Fe3+ and Cu2+, whereas red-emitting YCY-AuNC …

Public Horticulture: Process And Design Of The Lincoln Botanical Garden, Brad Kindler

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Beginning in 2012, the local non-profit, The Lincoln Botanical Garden, began organizing the conceptual design and creation of a glass conservatory within a botanical garden in Lincoln, Nebraska. These proposed urban enhancements seek to fill a gap in garden amenities in the city by providing year-round access to public horticulture programming and education.

This project, Public Horticulture: Process and Design of the Lincoln Botanical Garden, documents these efforts and makes design recommendations for a themed Glacial Erratic Garden that could be constructed within the botanical garden.

Advisors: Ellen Paparozzi and Sam Wortman

Stories Of Motivation And Values Of Small-Scale Farmers In Jamaica And The United States, Raquel Taylor

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Small-scale farmers add to global and local food systems through various means such as connecting communities to local food and support economies. Small-scale farms can address issues related to food and nutritional security. However, farming can be a challenging career to engage in, especially for those who are small-scale farmers. Matters related to succession, higher paying off-farm employment, policies, and more can make it challenging to engage in farming. Leaving the question of what would motivate someone to engage in small-scale farming. Motivation can be viewed through multiple lenses; this study chose to use self-determination theory to understand the motivations …

Impact Of Plant Defenses And Aphid-Associated Microbes In Modulating Sorghum–Sugarcane Aphid Interactions, Edith Ikuze

Department of Entomology: Dissertations, Theses, and Student Research

Sorghum (Sorghum bicolor) is a vital cereal crop globally, facing challenges from invasive pests such as the sugarcane aphid (SCA; Melanaphis sacchari). To elucidate mechanisms of sorghum resistance to SCA, we examined the role of plant defenses and the influence of aphid-associated microbes in modulating sorghum defenses. Lignin is a complex heterogenous polymer present in nearly all plant cell walls that plays a critical role in protecting plants from various stresses. In this study, we investigated the role of the caffeoyl coenzyme-A O-methyltransferase (CCoAOMT), one of the core enzymes in monolignol biosynthesis pathway that leads to lignin …

Breaking The Rule Of Silence: Childbirth And Gendered Power In Efuru And The Joys Of Motherhood, Sunday Elliott Uguru

Department of English: Dissertations, Theses, and Student Research

This study examines the thematic preoccupation of childbirth in the formative period of feminist discourse in African literature through a critical study of selected novels of Igbo women of southeastern Nigeria. The novels studied represent the earliest published African texts in English by women. The period under focus falls within the emerging stage of Nigerian literary tradition in its written form with a dominant presence of men. This study investigates the women novelists' perspective toward the failure of male authored works to represent women's childbirth experience. Through a critical reading of Flora Nwapa's Efuru and Buchi Emecheta's The Joys of …

A Fish Out Of Water: An Examination Of Self-Identified Introverted Business Students' Perceptions Of Their Leadership Ability, Katie J. Jolicoeur

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Current American cultural and societal ideals favor personalities that are outgoing, positive, and talkative in nature, due to the rise of the Culture of Personality (Wilmot, 2019; Kim, 2012; Cain, 2013). Beginning with the industrial revolution and expanding rapidly midcentury, parents began to view children who are quiet as less favorable than those who are more gregarious, thus children and parents began forced socialization at an earlier age (Wilmot, 2019; Cain, 2013). Very little, if any, research has been conducted to understand the impact that the cultural ideal of favoring extraversion has upon undergraduate students seeking a degree in business, …

Next-Generation Crop Monitoring Technologies: Case Studies About Edge Image Processing For Crop Monitoring And Soil Water Property Modeling Via Above-Ground Sensors, Nipuna Chamara

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Artificial Intelligence (AI) has advanced rapidly in the past two decades. Internet of Things (IoT) technology has advanced rapidly during the last decade. Merging these two technologies has immense potential in several industries, including agriculture.

We have identified several research gaps in utilizing IoT technology in agriculture. One problem was the digital divide between rural, unconnected, or limited connected areas and urban areas for utilizing images for decision-making, which has advanced with the growth of AI. Another area for improvement was the farmers' demotivation to use in-situ soil moisture sensors for irrigation decision-making due to inherited installation difficulties. As Nebraska …

Vibratory Noise Impacts The Sensory Ecology Of A Funnel-Weaving Spider, Brandi June Pessman

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Animals experience their world through diverse sensory systems, leveraging information from prey, predators, and potential mates to inform behavioral decisions. Environmental noise disrupts information via spectral or amplitude masking, degradation, or distortion, potentially leading to costly errors in responses. Critically, urbanization has accelerated the proliferation of novel noise sources, triggering significant shifts in communication, foraging, and predator-prey dynamics among urban wildlife. While vertebrate studies have illuminated urban animals’ flexibility to anthropogenic acoustic noise, a comprehensive understanding requires broader investigations across diverse animal taxa and sensory modalities. To address these gaps, my dissertation explored the impacts of anthropogenic substrate-borne (vibratory) noise …

Healing In The Workplace: School Counselors, Trauma, And Growth, Judith L. Sigler

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

An autoethnographic study on the experience of a Midwest rural school counselor represents a personal and professional perspective on trauma, burnout, and posttraumatic growth. Content includes rural adversity, grief, and career progression. The research aimed to examine professional and private adversities to inform and improve the practice of professional school counselors.

The following research questions were addressed:

1. How did personal and secondary trauma impact a rural school counselor’s professional trajectory?
2. What insight can be offered to other school counselors or educators experiencing the challenges that come with this work and, sometimes, that come from sources that are not from work …

Genome-Scale Metabolic Modeling Of Chitin-Degrading Microbial Systems, Aimee Kristin Kessell

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

As a major component of fungal cell walls and exoskeletons of invertebrates, chitin is widespread in soils, constituting the second most abundant biopolymer in nature. Composed of N-acetyl-D-glucosamine chains, it serves as a vital source of nutrients, including both carbon and nitrogen, for the growth of microorganisms. A solid understanding of the microbial degradation of chitin is critical for predicting their impacts on biogeochemical cycling in soil ecosystems. Organisms that degrade biopolymers (degraders) produce energetically expensive extracellular enzymes to break down complex organic carbons into simpler labile forms that are sharable with other species, including those that do not contribute …

Charge And Strain Engineering Of Ferroic And Correlated Oxides, Yifei Hao

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

This dissertation presents a comprehensive study of the charge and strain effects on the electronic and magnetic properties of epitaxial ferroic and correlated oxide thin films and heterostructures. We perform the planar Hall effect to quantify the magnetocrystalline anisotropy (MCA) in epitaxial La_1-xSr_xMnO₃ films (x = 0.2 and 0.33) deposited on SrTiO₃ substrates. The biaxial magnetic anisotropy energy of L_0.67Sr_0.33MnO₃ is about 17% larger than La_0.8Sr_0.2MnO₃. By comparing with the theoretically predicted values, we have decoupled the doping and strain effects on …

The Relationship Between Sex Education And Women’S Sexual Liberation And Exploration: A Phenomenological Study, Jessica Ann Boyles

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Sex education in schools is a pivotal yet contentious aspect of adolescent development, with enduring impacts on individuals' sexual exploration and liberation. However, the types of sex education offered have varied over time, often disseminating misinformation, and reinforcing traditional gender norms. Particularly for women, sex education can perpetuate harmful stereotypes, limit sexual agency, and contribute to disparities in sexual and reproductive health. This qualitative study aims to address the gap of intersecting sex education and sexual identity by examining the relationship between women's sexual liberation and exploration in adulthood and the sex education they received. Drawing from a phenomenological approach, …

The Sexual Trauma Of The Female Body: Violence Against Women In Contemporary Spanish And Latin American Cultures, Lara Armenteros Garrido

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

This dissertation focuses on the idea that women’s bodies have a sexual stigma attached to them as a consequence of different forms of heteropatriarcal violence inflicted on them. From violence in public spaces, such as street harassment or the sexual exploitation of women, to the intimacy of the relationship, like intimate partner violence or the orgasm gap, it becomes increasingly difficult for women to escape violence. Through the analysis of films, documentaries, narratives, and the testimonies of women from Spain and Latin America, I argue there is still a lack of adequate sexual and emotional knowledge to help complete agency …

Sacrificial Lambs: The Perils Of Childhood In Shakespeare, Benjamin Sidney Reed

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

“Sacrificial Lambs: The Perils of Childhood in Shakespeare” utilizes childhood studies to read five Shakespearean plays and how they portray child characters in perilous situations such as kidnapping, banishment, and murder. It argues these dangers emulate real circumstances young people faced in the early modern period, including the boy actors playing these roles. The dissertation’s key research questions include how children in these plays exhibit agency in the face of danger, how they resist adult systems of power, and how audiences in the sixteenth and seventeenth centuries may have responded to these portrayals of imperiled and rebellious youths. The Introduction …