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In Silico Conformation Analysis Of The Disordered Regions Of Proteins Associated With The Β-Catenin Destruction Complex And P53, Clifford Chen

Honors Scholar Theses

Cancer is a class of diseases caused by an accumulation of genetic mutations and abnormalities during cell division. These errors are caused by a variety of factors such as the inhibition of cell cycle control proteins such as p53 that prevent cells with incorrectly replicated chromosomes from segregating as well as genetic and environmental susceptibilities that further cause DNA alteration. Proteins like p53 are tumor suppressor proteins which prevent the growth of cancer cells by regulating the growth of cells as they pass through the cell cycle. Tumor suppressor proteins p53, Axin, Adenomatous polyposis coli (APC), and β-catenin were analyzed …

3d Bioprinting The Tumor Microenvironment For Immunotherapy Development, Tykeem Manor, Molly Leyda, John Szarejko, Douglas Myers, John M. Perry

Research at Children's Mercy Month 2024

The tumor microenvironment (TME) is a complex and dynamic system that plays a crucial role in tumor progression, immune evasion, and therapy resistance. The TME is composed of various cell types, including tumor cells, stromal cells, and immune cells, such as macrophages. Macrophages are versatile myeloid cells that can exhibit both protumoral and anti-tumoral functions, depending on their phenotype and the TME context. Classically activated M1 macrophages exhibit proinflammatory and tumoricidal functions and have demonstrated promising anti-tumor effects in immunotherapy research. Conversely, alternatively activated M2 macrophages have wide ranging functions in tissue repair and immune regulation. Tumor associated M2 macrophages …

Uni Impact: Faculty/Staff Scholarly Publications And Featured Awards Newsletter, V2i1, 2023, Rod Library. Research & Sponsored Programs. Uni Foundation. University Of Northern Iowa.

UNI Impact: Faculty/Staff Scholarly Publications and Featured Awards Newsletter

Highlights the scholarship, research, and other scholarly work produced by University of Northern Iowa faculty, students, and staff.

This issue covers the period of January - June 2023.

Gender. Identity. Property?, Eliot T. Tracz

UC Law SF Journal on Gender and Justice

No abstract provided.

Aged 539 Graduate Internship Report - Clovis East High School, Kaylee C. Santos

Agricultural Education: Graduate Internship Reports

This internship/project report includes documentation required in meeting the quality criteria for secondary-level programs of instruction in agriculture. The documents are concurrently used for the Agriculture Incentive Grant review process at Clovis East High School conducted by representatives of the California Department of Education. The supporting material includes information to receive state and local funding, outline the goals and objectives of the program, along with an overview of Clovis East High School, the agriculture program and the community. The project includes the revision of the Agrisciene Pathway Capstone Course Curriculum and Pacing Guide. This pacing guide will allow educators to …

Elucidating The Impact Of Casting Solvent On Vanadium Ion Transport In Solution-Cast Ionomer Biocomposites Containing Lignin, Zhongyu Wang

All Theses

This paper attempts to investigate the influence of solvent choice to the Nafion-lignin proton exchange membranes that used in the vanadium redox flow batteries. The influence of two different solvents: DMF and DMSO were investigated in this paper. Results show that when using DMSO as solvent, Nafion-lignin membranes' vanadium ion cross over can be greatly reduced in comparing with membranes prepared from DMF. Also, membranes prepared from DMSO also show higher water uptake ability and lower ion exchange capacity than the membranes prepared from DMF. This paper has also investigated the influence of lignin type to the membranes. Results show …

Illinois State University, One Hundred And Sixty-Fifth Annual Commencement, May 2024, Illinois State University

Commencement Programs

Illinois State University
One Hundred and Sixty-Fifth Annual Commencement
May 2024

Continual Learning For An Ever Evolving And Intelligent Malware Classification System, Mohammad Saidur Rahman

Theses

Malware classification poses unique challenges for continual learning (CL) systems, driven by the daily influx of new samples and the evolving nature of malware threats that exploit new vulnerabilities. Antivirus vendors encounter hundreds of thousands of unique software pieces daily, encompassing both malicious and benign files. Over its operational life, a malware classifier can accumulate more than a billion samples. Training malware classification system with only new samples and classes leads to catastrophic forgetting (CF), where the system forgets previously learned data distribution. While retraining with all old and new samples effectively combats CF, it is computationally expensive and necessitates …

Revitalizing The Rochester Gorge Strategic Urban Site Planning To Enhance Water Quality In The Lower Genesee River, Stephen Adam Stoker

Theses

The project began with investigating a regional asset of interest: the Genesee River. Initial research into this area revealed that the river suffers from poor water quality. The causes and effects of poor water quality were explored, and it was determined that excess nutrients – namely phosphorus – play a critical role in the water quality of the Genesee River and present downstream risk at the river’s point of outflow in Lake Ontario. An examination of peer-reviewed literature, contemporary examples in the built environment where similar water quality issues were addressed, and existing watershed design frameworks were conducted to understand …

Changes In Zooplankton Community Structure In A Wetland Metacommunity Following Homogenization From Flooding, Kevin Peteroy

Theses, Dissertations and Culminating Projects

Wetlands in urban areas provide ecosystem services that benefit the environment and society. Through these services, wetlands are able to help mitigate consequences of human activities such as flooding by increasing permeable surface coverage and contaminant filtering from polluted runoff. One such type of wetland, vernal pools, provide important temporary habitat for many organisms, including myriad zooplankton species. For planktonic organisms, flooding events might be disruptive or allow them to disperse to areas that would be otherwise inaccessible. I observed changing abiotic conditions and zooplankton communities in 20 vernal pools at a wetland in Wayne, New Jersey, USA over a …

Limited Interspecific Gene Flow In The Evolutionary History Of The Icefish Genus Chionodraco, Luca Schiavon, Santiago G. Ceballos, Michael Matschiner, Emiliano Trucchi, Mario La Mesa, Emilio Riginella, Magnus Lucassen, Felix C. Mark, Kevin Bilyk, Rafaella Franch, Andreas Wallberg, Elisa Boscari, Lorenzo Zane, Chiara Papetti

Department of Biology Faculty Scholarship and Creative Works

Hybridization and introgression are recognized as mechanisms promoting genetic variability during evolutionary radiations. We examined the impact of introgression in the process of speciation, focusing on the Antarctic icefish genus Chionodraco. Our analyses confirmed that the three Chionodraco species (Chionodraco hamatus, Chionodraco myersi, and Chionodraco rastrospinosus) were genetically distinctive, despite signals of past interspecific gene flow between C. hamatus and C. myersi that likely occurred during interglacial periods. However, in this study, no recent hybrids were identified. The lack of contemporary hybridization may be due to life-history traits and the type of marker used in the analysis. Our study emphasizes …

Interpretive Divergence In The New York Court Of Appeals, Ethan J. Leib

Journal of Legislation

This Article focuses attention on the New York Court of Appeals, which is decidedly formalist about contract interpretation but decidedly contextualist about statutory interpretation. It explores some recent exemplary cases to show where the New York Court of Appeals tends to land in what turns out to be, for this court at least, two different battlefields in the law of interpretation. Finding that there is “interpretive divergence” between statutory and contract cases, the Article then reflects on the practice of divergence more generally, revisiting assumptions about why anyone might have thought harmonization was sensible in the first place.

Optimization Of Collagen And Cell Concentrations For Engineered Tissues, Avery Fiser

Chemical Engineering Undergraduate Honors Theses

Improvements regarding the drug discovery process are necessary due to less than 1% of drugs making it from the initial discovery phase to patient treatment in clinics. One method of enhancing this process involves improving the relevance and dependability of in vitro testbeds utilized during pre-clinical testing. Improvement along these factors can provide a more relevant view for eventual drug behavior in patients. This additional relevance can allow for a reduced timeline in drug development for improving patient care. A consistent and morphologically sound baseline engineered tissue is vital to the creation of successful experimental models. Parameters such as cell …

Restricting Bioenergetic Efficiency Enhances Longevity And Mitochondrial Redox Capacity In Drosophila Melanogaster, Analisa L. Taylor, Olga Dubuisson, Pritika Pandey, Elizabeth R.M. Zunica, Bolormaa Vandanmagsar, Wagner S. Dantas, Alyssa Johnson, Christopher L. Axelrod, John P. Kirwan

Division of Basic Science Faculty Publications

Mitochondria are essential for survival and as such, impairments in organelle homeostasis significantly accelerate age-related morbidity and mortality. Here, we determined the contribution of bioenergetic efficiency to life span and health span in Drosophila melanogaster utilizing the mitochondrial uncoupler BAM15. Life span was determined in flies fed a normal diet (ND) or high fat diet (HFD) supplemented with vehicle or BAM15. Locomotor function was determined by negative geotaxis assay in middle-aged flies fed vehicle or BAM15 under ND or HFD conditions. Redox capacity (high-resolution respirometry/fluorometry), citrate synthase (enzyme activity), mtDNA content (qPCR), gene expression (qPCR), and protein expression (western blot) …

Integration Of Adverse Childhood Experiences In Adulthood Through Dance Movement Therapeutic Techniques, Kimberly Hoj

Expressive Therapies Capstone Theses

Expanding knowledge about how to self-regulate when faced with challenging circumstances has the potential to benefit individuals and communities, especially in our post-pandemic environment with increasing needs for mental health support. This thesis provides a psychoeducational basis for use of dance movement therapeutic techniques to integrate adverse childhood experiences. The literature review refines an understanding of big T and little t trauma as it relates to mental illness, polyvagal theory, nervous system regulation, the window of tolerance, body armoring, somatic psychotherapy, trauma- informed care, adverse childhood experiences and post-traumatic growth.

This thesis culminates in a personal arts-based autoethnography informed by …

Gardner-Webb Honors Experience, July 14-19, Introduces Qualified High School Students To College Life, Office Of University Communications

Gardner-Webb NewsCenter Archive

The Honors Experience at Gardner-Webb University this summer invites students to explore an array of topics through innovative learning techniques. Offered July 14-19, the program gives high-achieving high school students an opportunity to experience all aspects of life on the GWU campus from attending classes and chapel to staying in residence halls and eating at the many campus dining options.

Dance/Movement Therapy And The Beginnings Of Decolonizing The Post-Restraint Process: A Literature Review, Marissa Jax

Expressive Therapies Capstone Theses

Restraints are used to ensure and provide safety across populations and in a multitude of settings. It is also acknowledged that restraints are at a high risk of causing trauma for all involved in the restraint process (Cui, et al., 2021; Dotson et al., 2022; Rose & Slack, 2016; Singal et al., 2023). There is an abundance of research on how the body is affected by trauma (Barnstaple & Dieterich-Hartwell, 2022; Levine, 2010; Maté, 2022; Menakem, 2017; Mullan, 2023; van der Kolk, 2014). Trauma from restraints can disproportionately affect people of color and indigenous peoples (Dotson et al., 2022; Linklater, …

Dystopian Novels And The Greater Boston Area: A Reflection On Privilege, Leonersy J. Guerrero

English Honors Theses

Dystopian fiction has long been a vehicle for exploring societal issues and envisioning potential futures. Although many view the genre as a form of escapism, these novels have the potential to make grand statements about the nature of humanity. From George Orwell's 1984 to Octavia Butler's Parable of the Sower, these narratives serve as a reflection of our current societal standing and project potential consequences of our collective actions. However, within the realm of dystopian literature, there exists a significant gap in equal representation of the experiences and unique perspectives of the Black community. Specifically, within the context of …

Exploring Peer Perspectives On Autistic Students: A Vignette Study, Justin Linville

Electronic Theses and Dissertations

A 2x2 design was undertaken to determine the effects of vignette character gender and autism disclosure status on social distance scores, reading comprehension performance, and false memory induction. The introduction focuses on the theoretical threads taken from the autism, false memory, and gender literatures and how they combine to create the underpinning of the current design. A second more controlled study was compelled by initial false impressions of the deleterious effects of fast completion times. This second study resulted in lengthened completion times and improved vignette comprehension. Two-way MANOVAs with gender and autism disclosure serving as independent variables on the …

Detection Of Ochyromera Ligustri (Coleoptera: Curculionidae: Curculioninae) In Ligustrum Spp. (Oleaceae) Using Newly Developed Pcr Primers, Ilgoo Kang, Amy Roda, Brandi Misiaszek, Tanner Sparks, Rodrigo Diaz

Faculty Publications

Ligustrum spp. (Oleaceae) have become invasive species in the US and negatively affect native plant diversity and richness in forests. Ochyromera ligustri (Coleoptera: Curculionidae) is considered a potential biological control agent in the US because adults feed on the foliage and larvae are seed-feeders of Ligustrum spp. To discover the relationships between O. ligustri and Ligustrum spp., fruit dissections or rearing and field observations are required. In the current research project, novel PCR primers were developed to rapidly detect the DNA of O. ligustri in molecular analyses without rearing and observation. The developed PCR primers worked even with 0.01 ng …

Mapping And Omics Integration: Towards Precise Rice Disease Resistance Breeding, John Christian Ontoy, Jong Hyun Ham

Faculty Publications

Rice (Oryza sativa), as a staple crop feeding a significant portion of the global population, particularly in Asian countries, faces constant threats from various diseases jeopardizing global food security. A precise understanding of disease resistance mechanisms is crucial for developing resilient rice varieties. Traditional genetic mapping methods, such as QTL mapping, provide valuable insights into the genetic basis of diseases. However, the complex nature of rice diseases demands a holistic approach to gain an accurate knowledge of it. Omics technologies, including genomics, transcriptomics, proteomics, and metabolomics, enable a comprehensive analysis of biological molecules, uncovering intricate molecular interactions within the rice …

Exploring Pathways Toward Open-Hardware Ecosystems To Safeguard Genetic Resources For Biomedical Research Communities Using Aquatic Model Species, Yue Liu, Jack C. Koch, Lucía Arregui, Allyssa Oune, Sarah Bodenstein, Maria T. Gutierrez-Wing, Terrence R. Tiersch

Faculty Publications

Development of reliable germplasm repositories is critical for preservation of genetic resources of aquatic species, which are widely utilized to support biomedical innovation by providing a foundational source for naturally occurring variation and development of new variants through genetic manipulations. A significant barrier in repository development is the lack of cryopreservation capability and reproducibility across the research community, posing great risks of losing advances developed from billions of dollars of research investment. The emergence of open scientific hardware has fueled a new movement across biomedical research communities. With the increasing accessibility of consumer-level fabrication technologies, such as three-dimensional printers, open …

Sexual Orientation-Based Microaggressions And Cannabis Use Outcomes Among Sexual Minority Individuals: The Impact Of Negative Affect And Coping-Motivated Cannabis Use, Evan M. Threeton, Paige E. Morris, Julia D. Buckner

Faculty Publications

Introduction: Sexual minority individuals report significantly more cannabis use and problems than their heterosexual peers, possibly due to their frequent experiences with sexual orientation-based microaggressions. As a result, sexual minority individuals may turn to cannabis use to cope with the negative affect associated with these experiences. No known studies have tested this hypothesis; therefore, the current study tested if sexual orientation microaggressions are positively associated with negative affect (anxiety and depression), cannabis coping motives, and cannabis-related outcomes (frequency, problems); if coping motives are positively associated with cannabis-related outcomes; and if negative affect and cannabis use to cope with negative affect …

Kat8 Beyond Acetylation: A Survey Of Its Epigenetic Regulation, Genetic Variability, And Implications For Human Health, Lindsey Yoo, David Mendoza, Allison J. Richard, Jacqueline M. Stephens

Division of Basic Science Faculty Publications

Lysine acetyltransferase 8, also known as KAT8, is an enzyme involved in epigenetic regulation, primarily recognized for its ability to modulate histone acetylation. This review presents an overview of KAT8, emphasizing its biological functions, which impact many cellular processes and range from chromatin remodeling to genetic and epigenetic regulation. In many model systems, KAT8’s acetylation of histone H4 lysine 16 (H4K16) is critical for chromatin structure modification, which influences gene expression, cell proliferation, differentiation, and apoptosis. Furthermore, this review summarizes the observed genetic variability within the KAT8 gene, underscoring the implications of various single nucleotide polymorphisms (SNPs) that affect its …

Transitioning From A Research Protocol To A Scalable Applied Pathway For Xenopus Laevis Sperm Cryopreservation At A National Stock Center: The Effect Of Cryoprotectants, Lucía Arregui, Jack C. Koch, Terrence R. Tiersch

Faculty Publications

Sperm cryopreservation is a critical tool for safeguarding and managing valuable genetic resources. Protocols for cryopreservation of Xenopus laevis sperm were available but lacking sperm quality evaluation and scalability and the outcomes were inconsistent. The goal of this study was to begin developing a center-level cryopreservation pathway for this species by integrating French straws as containers that would facilitate germplasm repository development. The objectives were to analyze the effect of: (1) three sperm concentrations (33, 50, and 100 × 106 sperm/mL) on post-thaw fertilization, (2) three final concentrations (2.5%, 5%, and 10%) of dimethyl sulfoxide, methanol, and dimethylformamide (DMFA) on …

Energy Compensation For Crop Growth Under Plastic Mulching: Theories, Models, And Limitations, Dianyuan Ding, Ting Li, Lihong Wu, Xi Zhang, Ying Zhao, Hao Feng, Chao Zhang, Ole Wendroth

Faculty Publications

Plastic film mulching (PM) is a useful agronomic means to adapt to the environmental conditions of dryland agriculture and improve crop production. To improve the theoretical framework of PM technology, this study focuses on the interaction between the soil temperature change caused by PM and crop growth. The definition, action mechanism, and simulation of the compensatory effect of PM on growing degree days are introduced to reveal the effect of soil temperature under PM on crop development and growth. Our summary shows that the strength of the warming effect changes with the growth and development of crops, strengthening during the …

Song And Genetic Divergence Within A Subspecies Of White-Crowned Sparrow (Zonotrichia Leucophrys Nuttalli), Amy Rongyan Luo, Sara Lipshutz, Jennifer Phillips, Robb T. Brumfield, Elizabeth Perrault Derryberry

Faculty Publications

Animal culture evolves alongside genomes, and the two modes of inheritance-culture and genes-interact in myriad ways. For example, stable geographic variation in culture can act as a reproductive barrier, thereby facilitating genetic divergence between "cultural populations."White-crowned sparrows (Zonotrichia leucophrys) are a well-established model species for bird song learning and cultural evolution, as they have distinct, geographically discrete, and culturally transmitted song types (i.e., song dialects). In this study, we tested the hypothesis that divergence between culturally transmitted songs drives genetic divergence within Nuttall's white-crowned sparrows (Z. l. nuttalli). In accordance with sexual selection theory, we hypothesized that cultural divergence between …

Associations Of Epigenetic Age Acceleration With Cvd Risks Across The Lifespan: The Bogalusa Heart Study, Xiao Sun, Wei Chen, Alexander C. Razavi, Mengyao Shi, Yang Pan, Changwei Li, Maria Argos, Brian T. Layden, Martha L. Daviglus, Jiang He, Owen T. Carmichael, Lydia A. Bazzano, Tanika N. Kelly

Clinical Research Faculty Publications

Although epigenetic age acceleration (EAA) might serve as a molecular signature of childhood cardiovascular disease (CVD) risk factors and further promote midlife subclinical CVD, few studies have comprehensively examined these life course associations. This study sought to test whether childhood CVD risk factors predict EAA in adulthood and whether EAA mediates the association between childhood CVD risks and midlife subclinical disease. Among 1,580 Bogalusa Heart Study participants, we estimated extrinsic EAA, intrinsic EAA, PhenoAge acceleration (PhenoAgeAccel), and GrimAge acceleration (GrimAgeAccel) during adulthood. We tested prospective associations of longitudinal childhood body mass index (BMI), blood pressure, lipids, and glucose with EAAs …

The Cospar Planetary Protection Policy For Missions To Icy Worlds: A Review Of History, Current Scientific Knowledge, And Future Directions, P. T. Doran, A. Hayes, O. Grasset, A. Coustenis, O. Prieto-Ballesteros, N. Hedman, O. Al Shehhi, E. Ammannito, M. Fujimoto, F. Groen, J. E. Moores, C. Mustin, K. Olsson-Francis, J. Peng, K. Praveenkumar, P. Rettberg, S. Sinibaldi, V. Ilyin, F. Raulin, Y. Suzuki, K. Xu, L. G. Whyte, M. Zaitsev, J. Buffo, G. Kminek, B. Schmidt

Faculty Publications

Recent discoveries related to the habitability and astrobiological relevance of the outer Solar System have expanded our understanding of where and how life may have originated. As a result, the Icy Worlds of the outer Solar System have become among the highest priority targets for future spacecraft missions dedicated to astrobiology-focused and/or direct life detection objectives. This, in turn, has led to a renewed interest in planetary protection concerns and policies for the exploration of these worlds and has been a topic of discussion within the COSPAR (Committee on Space Research) Panel on Planetary Protection. This paper summarizes the results …

Complementing Model Species With Model Clades, Makenzie E. Mabry, R. Shawn Abrahams, Ihsan A. Al-Shehbaz, William J. Baker, Simon Barak, Michael S. Barker, Russell L. Barrett, Aleksandra Beric, Samik Bhattacharya, Sarah B. Carey, Gavin C. Conant, John G. Conran, Maheshi Dassanayake, Patrick P. Edger, Jocelyn C. Hall, Yue Hao, Kasper P. Hendriks, Julian M. Hibberd, Graham J. King, Daniel J. Kliebenstein, Marcus A. Koch, Ilia J. Leitch, Frederic Lens, Martin A. Lysak, Alex C. Mcalvay, Michael T.W. Mckibben, Francesco Mercati, Richard C. Moore, Klaus Mummenhoff, Daniel J. Murphy, Lachezar A. Nikolov, Michael Pisias, Eric H. Roalson

Faculty Publications

Model species continue to underpin groundbreaking plant science research. At the same time, the phylogenetic resolution of the land plant tree of life continues to improve. The intersection of these 2 research paths creates a unique opportunity to further extend the usefulness of model species across larger taxonomic groups. Here we promote the utility of the Arabidopsis thaliana model species, especially the ability to connect its genetic and functional resources, to species across the entire Brassicales order. We focus on the utility of using genomics and phylogenomics to bridge the evolution and diversification of several traits across the Brassicales to …