Digital Commons Network^™

The Clinical Utility And Diagnostic Implementation Of Human Subject Cell Transdifferentiation Followed By Rna Sequencing, Shenglan Li, Sen Zhao, Jefferson C Sinson, Aleksandar Bajic, Jill A Rosenfeld, Matthew B Neeley, Mezthly Pena, Kim C Worley, Lindsay C Burrage, Monika Weisz-Hubshman, Shamika Ketkar, William J Craigen, Gary D Clark, Seema Lalani, Carlos A Bacino, Keren Machol, Hsiao-Tuan Chao, Lorraine Potocki, Lisa Emrick, Jennifer Sheppard, My T T Nguyen, Anahita Khoramnia, Paula Patricia Hernandez, Sandesh Cs Nagamani, Zhandong Liu, Christine M Eng, Brendan Lee, Pengfei Liu

Faculty, Staff and Students Publications

RNA sequencing (RNA-seq) has recently been used in translational research settings to facilitate diagnoses of Mendelian disorders. A significant obstacle for clinical laboratories in adopting RNA-seq is the low or absent expression of a significant number of disease-associated genes/transcripts in clinically accessible samples. As this is especially problematic in neurological diseases, we developed a clinical diagnostic approach that enhanced the detection and evaluation of tissue-specific genes/transcripts through fibroblast-to-neuron cell transdifferentiation. The approach is designed specifically to suit clinical implementation, emphasizing simplicity, cost effectiveness, turnaround time, and reproducibility. For clinical validation, we generated induced neurons (iNeurons) from 71 individuals with primary …

De Novo Genome Assembly For The Coppery Titi Monkey (Plecturocebus Cupreus): An Emerging Nonhuman Primate Model For Behavioral Research, Susanne P Pfeifer, Alexander Baxter, Logan E Savidge, Fritz J Sedlazeck, Karen L Bales

Faculty, Staff and Students Publications

The coppery titi monkey (Plecturocebus cupreus) is an emerging nonhuman primate model system for behavioral and neurobiological research. At the same time, the almost entire absence of genomic resources for the species has hampered insights into the genetic underpinnings of the phenotypic traits of interest. To facilitate future genotype-to-phenotype studies, we here present a high-quality, fully annotated de novo genome assembly for the species with chromosome-length scaffolds spanning the autosomes and chromosome X (scaffold N50 = 130.8 Mb), constructed using data obtained from several orthologous short- and long-read sequencing and scaffolding techniques. With a base-level accuracy of ∼99.99% in chromosome-length …

The Long Non-Coding Rna Gene C027288.3 Plays A Role In Human Endometrial Stromal Fibroblast Decidualization, Rupak Thapa, Kevin Marmo, Liang Ma, Donald S Torry, Brent M Bany

2020-Current year OA Pubs

During the secretory phase of the menstrual cycle, endometrial fibroblast cells begin to change into large epithelial-like cells called decidual cells in a process called decidualization. This differentiation continues more broadly in the endometrium and forms the decidual tissue during early pregnancy. The cells undergoing decidualization as well as the resulting decidual cells, support successful implantation and placentation during early pregnancy. This study was carried out to identify new potentially important long non-coding RNA (lncRNA) genes that may play a role in human endometrial stromal fibroblast cells (hESF) undergoing decidualization in vitro, and several were found. The expression of nine …

A Genetic Association Study Of Circulating Coagulation Factor Viii And Von Willebrand Factor Levels, Paul S. De Vries, Paula Reventun, Michael R. Brown, Adam S. Heath, Jennifer E. Huffman, Ngoc Quynh Le, Allison Bebo, Jennifer A. Brody, Gerard Temprano-Sagrera, John Blangero

School of Medicine Publications

Coagulation factor VIII (FVIII) and its carrier protein von Willebrand factor (VWF) are critical to coagulation and platelet aggregation. We leveraged whole-genome sequence data from the Trans-Omics for Precision Medicine (TOPMed) program along with TOPMed-based imputation of genotypes in additional samples to identify genetic associations with circulating FVIII and VWF levels in a single-variant meta-analysis, including up to 45 289 participants. Gene-based aggregate tests were implemented in TOPMed. We identified 3 candidate causal genes and tested their functional effect on FVIII release from human liver endothelial cells (HLECs) and VWF release from human umbilical vein endothelial cells. Mendelian randomization was …

Increasing Motivation In Stem Education: A Self-Talk Classroom Intervention, Tiffany L. Copeland, Richard Allen, Sofia Pham, Kristin Gagnier

Research Day

INTRODUCTION: This study examines a social-emotional learning intervention that teaches positive self-talk to high school students from underrepresented minority groups in science, technology, engineering, and math (STEM). The setting of this study is a place-based program that immerses students in hands-on STEM activities while located in Philadelphia’s life science hub. Before taking a science assessment, students will learn to say self-talk statements such as “I will give my best effort” and “I can achieve a high score.”

METHODS: This mixed method quasi-experimental design compares pre- and post-program data using an ANOVA. Student science performance will be measured using a probe …

Characterizing Potentially Harmful Environmental Legacy Issues Within A Declining Midwest City: A Street Sediment Study Of Northeastern Terre Haute, Indiana, Katelyn Adams '24, Ken Brown

Senior Scholarly and Creative Symposium

Urban settings face unprecedented environmental challenges that include legacy issues as well as emerging pollution problems (e.g., micro-plastics) that continually impact the public health of its communities. Determining the baseline abundances of potentially harmful contaminants in soil, sediment, air, and water is essential to protecting residents. This study examines anthropogenic pollution in street sediments collected from three neighborhoods located in northeast Terre Haute, Indiana. Using stereomicroscopy, SEM/EDS, pXRF analyses, and geospatial analysis (GIS), the goal of this work is to characterize the types of pollutants present and place important constraints on their potential sources.

Stereomicroscopy on nine street sediment samples …

Peer Educator Recognition & Awards Banquet, April 17, 2024 [Program], University Of Northern Iowa. Undergraduate Studies Program.

Graduation with Distinction Posters

The program for the 2024 Peer Educator Recognition & Awards Banquet held on April 17, 2024.

"Fearfully And Wonderfully Made": The Reconciliation Of The Lgbtqia+ Community To American Christianity, Madalyn Sailors '24

Senior Scholarly and Creative Symposium

I explore in this paper the ways in which churches in the United States treat LGBTQIA+ issues and members in their congregations. To do so, I will focus on several case studies, including the Metropolitan Community Church, the Episcopal Church, the Roman Catholic Church, the Church of Jesus Christ of Latter-Day Saints, and the United Methodist Church. These examples range from socially affirming to conservative and from traditional to contemporary worship styles. I plan to research the social landscapes of these churches and propose possible solutions for how other faith spaces can create more inclusive membership. I also address my …

Towards A Sustainable Planet: Review Of ‘Global Environmental Politics’ By Kütting And Herman (2018), Mona Safizadeh

Markets, Globalization & Development Review

Global Environmental Politics by Kütting and Herman (2018) explores our planet's complex challenges, urging collective action for sustainability. Across 13 chapters, the book explores sustainable consumption, the role of technology, and the impact of evolving economies on global politics. This comprehensive guide emphasizes the interconnectedness of environmental, social, and economic factors, offering insights for stakeholders to navigate toward a healthier future.

Can Marketing Transcend Entrenched Gender Biases?, Thinh Nguyen

Markets, Globalization & Development Review

Through a feminist lens, Maclaran and Chatzidakis (2022) challenge conventional assumptions about gender, emphasizing its performative nature shaped by social practices rather than inherent traits. This commentary extends analyses of key themes such as gender performativity, the male gaze, and subject-object binaries within marketing. It critiques how marketing strategies reinforce existing power imbalances and systemic biases rooted in historical narratives. The writing also reflects on media interpretations of gender issues through films like 'Turning Red' and 'Barbie'. By contextualizing gendered marketing within broader societal frameworks, this writing contributes to ongoing dialogues in media studies, sociology, and gender studies, highlighting the …

Rapid Increase Of Female But Not Male Obesity: Analysis Of The 2023 Vanuatu Health Transition Project Survey On Aneityum, Matthew Christian, Olivia Lasalle, Zhiqiao Huang, Hannah Chen, Ricky Chen, J. Koji Lum

Binghamton University Undergraduate Journal

Globally, obesity rates are continuing to increase and countries in the midst of modernization are most vulnerable. Developing nations are undergoing a health transition alongside rapid economic modernization. The nation of Vanuatu, like other Pacific island countries, is experiencing such a transition marked by decreased cases of infectious disease and increased incidence of chronic and non-communicable diseases. Aneityum is a small and sparsely populated island in Vanuatu and is behind more developed islands in its transition. This present study is the latest in a multi-year project examining health in Vanuatu as it undergoes a health transition with an increased prevalence …

Measurement Of Transmission Efficiency Of Blue Light Blocking Devices, Jada Lee

Honors Theses

Technology and light sources have experienced a revolution in recent years leading to the production of light emitting diode (LED) bulbs. White-light LED bulbs undergo degradation over time, leading to a rise in color temperature and a proportional increase in the emission of blue light from these bulbs. The small size of LEDs makes them the optimal choice for electronic devices because of their limited screen size. This means that blue light now exists in red, green, and blue solid-state illumination systems that did not exist a decade ago. It is debated if blue light induces toxic effects on the …

Post-Mucilage Status Of The Teleost Fish Assemblages In The Sea Of Marmara, Mustafa Sari, Uğur Karadurmuş

Turkish Journal of Zoology

A frightening mucilage event occurred in the Sea of Marmara (SoM) from November 2020 to July 2021, leaving devastating effects on maritime industries, fishing activities and the benthic ecosystem. The mucilage led to intense fish mortalities, particularly affecting species like smelt, anchovy, and picarel, with severe consequences for benthic ecosystems and various marine species. This study aims to analyze the possible long-term effects of the recent mucilage disaster on species diversity and biomass by assessing the current status of teleost fish assemblages in the SoM. The data set is based on bottom trawl surveys carried out at 52 points at …

Occlusal Surface Variations In Genetically-Identified Specimens Of The Genus Apodemus (Mammalia: Rodentia) Distributed In The Northern Anatolia Region And Three Turkish Islands: Gökçeada, Marmara Island And Bozcaada, Duygu Korkmaz, Engin Selvi̇, Nuri Yi̇ği̇t, Ercüment Çolak

Turkish Journal of Zoology

A total of 134 Apodemus samples, whose genetic diagnoses had been previously conducted, were morphologically examined from 39 localities in Northern Anatolia, Thrace, Gökçeada, Bozcaada and the Marmara Island. The variation boundaries of dental variations in the distribution areas of five Apodemus species (Apodemus flavicollis, Apodemus witherbyi, Apodemus sylvaticus, Apodemus uralensis, Apodemus mystacinus) included in the research were determined. The defining morphological characters of Apodemus species, which have a complex taxonomic status, and their variations according to regions were identified using samples that had undergone genetic diagnoses. It was determined whether geographical barriers such as the Marmara Sea, the Bosphorus …

The Common Wall Lizard Podarcis Muralis (Reptilia: Lacertidae) Shows Diverse Food Preferences And Intraspecific Difference: A Study Case From Bulgaria, Emiliya Vacheva, Borislav Naumov

Turkish Journal of Zoology

The Common wall lizard (Podarcis muralis) has the largest range among the genus Podarcis. Although there are a number of studies on its diet, data on regional level remains insufficient, particularly from a purely ecological perspective. We investigated 194 individual faecal samples of P. muralis obtained from two localities in western Bulgaria with the aim of clarifying the composition of the trophic spectrum of the species, as well as the intra-population characteristics in terms of age and sex. In one study site we compared the realized trophic niche (prey items from the faecal pellets) with the fundamental (invertebrates collected via …

Sexual Size Dimorphism Of Darevskia Schaekeli (Sauria: Lacertidae), Seyyed Saeed Hosseinian Yousefkhani, Saeed Morvari

Turkish Journal of Zoology

Sexual dimorphism has been established as one of the important evolutionary results in the process of sexual selection between males and females of different species. Distinct morphological features between males and females can also be seen in reptiles. Lizards of the genus Darevskia show sexual dimorphism, as Darevskia schaekeli was studied as an example. The morphological traits investigated between males and females of this species showed that head length, trunk length, length of forelimbs and hindlimbs as well as the number of femoral pores are more in males than in females. All these morphological traits, which were more in males …

Class Of 2024 Commencement Program, State University Of New York College At Cortland

Commencement Programs

May Commencement, Graduate Ceremony

Improving Initial Management Of Acute Musculoskeletal Pain In The Emergency Department, Cynthia Y. Norris

Cohort XII

Background/Problem Statement: Acute musculoskeletal pain is one of the major chief complaints amongst Emergency Department (ED) visits each year. In the United States, approximately 41.9 million annual ED visits are musculoskeletal pain related (Fontánez, et al., 2021). Yet, data statistics demonstrate at least 40% of patients reported their pain remained unchanged during their presentation to an ED (Bussey et al., 2020).

Aims/Methods: At University Medical Center, site of the quality improvement project, a total of 91,455 ED annual visits were reported in the latest data reports (El Paso County Hospital District 2022 Annual Report, 2022). This paper chronicles the implementation …

Examining Social And Academic Integration Of International Students From Saudi Arabia And Mexico Attending Universities In The United States, Faisal Alqadiri

Theses & Dissertations

This study examined the problems and challenges of social and academic integration in U.S. higher education institutions for Saudi Arabian and Mexican undergraduate international students. The purpose of this quantitative, cross-sectional, descriptive, and causal-comparative study was to examine the relationship among (a) demographic variables (i.e., age, gender, education, marital status, nationality, and previous academic performance), (b) academic integration, (c) social integration, and (d) intent to drop out among Saudi and Mexican students studying at U.S. colleges and universities. In addition, the study compared whether Saudi and Mexican female students were less integrated academically and socially than their male counterparts. Previous …

Exploration Of Teachers’ Opinions Regarding The Instructional And Extracurricular Activities To Develop Transformational Leadership Qualities In Middle And Secondary School Students In Saudi Arabia, Mona Alkathiri

Theses & Dissertations

Research Focus. The purpose of the current study was to examine the opinions of KSA teachers regarding the appropriate instructional, extra-curricular, and understanding activities that pertain to the development of leadership, social skills, and personal traits of KSA secondary school students.

Research Methods. This study employed a qualitative, case study research design as an exploratory method of analyzing how transformational leadership principles could be the central organizing principle reorganizing the Kingdom of Saudi Arabia (KSA) public school system by 2030. A purposive sample of 18 active secondary school participants were recruited from a population of teachers in middle and secondary …

Women In Weights: Contraindications Of Cycle Syncing In Osteosarcopenia, Erin Underwood

University Honors College

The purpose of this scientific research was to examine the holistic health practice of women’s cycle syncing utilizing an in-depth analysis scope of non-pharmacological practices to prevent osteosarcopenia. Osteosarcopenia is the duplicate diagnosis of osteoporosis, low bone mineral density, and sarcopenia, low muscle mass, co-existing together. Cycle syncing is a media-popularized women’ reproductive health practice promoting to balance and regulate hormonal cycles holistically. This practice causes concern for fitness professionals because each menstrual cycle phase is treated differently regarding exercise and nutrition intake, which directly impacts energy levels, hormonal peaks, and overall bodily function in women. The focus of this …

Mental Health And Traumatic Occupational Exposure In Wildland Fire Dispatchers, Robin M. Verble, Rachel Granberg, Seth Pearson, Charlene Rogers, Roman Watson

Biological Sciences Faculty Research & Creative Works

Wildland Fire Dispatchers Play a Key Role in Wildland Fire Management and Response Organization; However, to Date, Wildland Fire Studies Have Largely Focused on the Physical Hazards And, to a Lesser Extent, Mental Health Hazards of Wildland Firefighting Operational Personnel, and Dispatcher Studies Have Primarily Focused on 911 and Police Dispatchers. Studies of Other Dispatchers Have Provided Some Limited Insight into Potential Strains Impacting This Workforce, Including Work-Related Fatigue, Burnout, and Traumatic Exposure. However, the Specific Job Hazards that Are Faced by Wildland Fire Dispatchers Are Poorly Understood. in 2023, We Conducted a Cross-Sectional Survey of 510 Wildland Fire Dispatchers …

Effectiveness Of Marine Species At Risk Conservation Within The Unep Regional Seas Programme: Taking Stock And Charting Future Courses, Olga Koubrak

PhD Dissertations

The impending biodiversity crises demands urgent, effective action. The transboundary nature of many marine species at risk makes international law a necessary tool in this endeavour. The United Nations Environment Programme and its Regional Seas Programme consists of 18 individual progammes spanning the globe and bringing together 143 countries in regional collaborations. This research project evaluates potential effectiveness of four programmes within the Regional Seas Programme relative to each other on twelve elements looking at legal and institutional structure, as well as regional implementation. The four case studies cover the North-East Atlantic, Mediterranean, East Africa, and Caribbean regions. These programmes …

The Benefits Of Using Open-Note Assessments, Logan Schaefers

Master of Education Program Theses

This action research study investigated the impact of open-note assessments on student learning as measured by the student’s overall score on unit assessments inside of a 9th grade Algebra 1 classroom. This study included 332 freshmen: an experimental group (open-note assessments) of 72 students and a control group of 260 students. Findings of this study did not show a statistically significant increase in the scores of students using open-note assessments. However, when looking at the different quartiles of test scores, the overwhelming evidence by the researcher revealed the benefits that open-note assessments had on student’s academic performance resulting in better …

Alcohol Reward Memory In Drosophila: Investigating Changes In Neural Activity In Response To Cues Alcohol Associations And Their Role In Regulating Other Alcohol-Related Behaviors., Marieke Guitink

Honors Projects in Biological and Biomedical Sciences

With more than 200 health conditions associated with Alcohol Use Disorder, excessive drinking can take a serious toll on an individual's health, mental health, and relationships. Despite significant disruptions, there are a few treatments for Alcohol Use Disorder that support long-term abstinence. To develop effective long-term treatments for Alcohol Use Disorder, it is essential to obtain an understanding of the underlying complex changes that alcohol has on the brain, however, the complexity of the mammalian nervous system has precluded researchers from achieving this. Drosophila melanogaster is a powerful model organism for investigating how alcohol hijacks neural circuits within the brain …

Understanding The Public Reaction To Major United States Environmental Policies Through Twitter, Ryan Giammarco

Honors Projects in Data Science

An increased focus on access to general data as well as a continued lack of usable environmental data have resulted in an odd phenomenon where the public does not have the opportunity to understand their environment on a deep level. The goal of this research is to understand, as a result, how people both talk and feel about certain environmental changes, particularly those in the realm of politics. Through word clouds and sentiment analysis performed with historical Twitter data collected between 2010 and 2022, we can identify the general trends in both conversation and feeling as they relate to a …

Cover And Contents

Turkish Journal of Zoology

No abstract provided.

Evaluating The Integration Of Traditional And Western Medicine In Rural Ghana: The Role Of Healers And The Government, T. Nicole Lemon

Anthropology Undergraduate Senior Theses

A question of pressing importance for the healthcare system in Ghana is the integration between biomedical physicians, who are unreachable for many rural citizens, and traditional healers, who fill in the gaps in access for rural and non-rural citizens seeking care, and are oftentimes the preferred choice. The care offered by each system is derived from differing paradigms, with the biomedical related to Westernized practices and the healing related to holistic, traditional approaches. Integration of these systems would allow for more acknowledgment and communication between these two different kinds of providers, which in turn would improve the quality of care …

Uncovering Novel Small Regulatory Rna In Protostome, Sweta Khanal

Dissertations

Small RNAs play pivotal roles in post-transcriptional gene regulation across diverse phylum of protostomes. In this study, we investigate the functional significance of atypical miRNAs, mirtron miR-1017 in Drosophila. Through ectopic expression in neuronal cells, we demonstrate that miR-1017 extends lifespan by targeting its host transcript, acetylcholine receptor Dα2, and influencing its splicing. This novel trans-regulatory function suggests a mechanism for mirtron evolution, highlighting the interplay between splicing and post-transcriptional regulation. Additionally, we profile small RNA populations in the polychaete developmental model Capitella teleta, shedding light on the small RNA landscape in annelid worms. Our analysis reveals a rich …

Advancing Sentiment Analysis Through Emotionally-Agnostic Text Mining In Large Language Models (Llms), Jay Ratican, James Hutson

Faculty Scholarship

The conventional methodology for sentiment analysis within large language models (LLMs) has predominantly drawn upon human emotional frameworks, incorporating physiological cues that are inherently absent in text-only communication. This research proposes a paradigm shift towards an emotionallyagnostic approach to sentiment analysis in LLMs, which concentrates on purely textual expressions of sentiment, circumventing the confounding effects of human physiological responses. The aim is to refine sentiment analysis algorithms to discern and generate emotionally congruent responses strictly from text-based cues. This study presents a comprehensive framework for an emotionally-agnostic sentiment analysis model that systematically excludes physiological indicators whilst maintaining the analytical depth …