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Development And Application Of Small-Molecule Probes Targeting Ghrelin O-Acyltransferase, Jacob Moose May 2024

Development And Application Of Small-Molecule Probes Targeting Ghrelin O-Acyltransferase, Jacob Moose

Dissertations - ALL

Ghrelin is a 28-amino acid peptide hormone that has wide-ranging physiological effects, including on metabolism, adipogenesis, insulin regulation, and addiction. Ghrelin offers an intriguing option for treatment of pathophysiologies related to these effects, as it requires a unique post-translational octanoyl modification to bind and activate its cognate receptor, growth hormone secretagogue 1a (GHSR). The enzyme responsible for this modification, ghrelin O-acyltransferase (GOAT) therefore offers the ability to modulate ghrelin signaling through inhibition of the maturation pathway. Due to a lack of structural or mechanistic information about GOAT, pharmaceutical development of molecules capable of GOAT inhibition has been desired, but few …

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Engineering The Signal Resolution Of A Paper-Based Cell-Free Glutamine Biosensor With Genetic Engineering, Metabolic Engineering, And Process Optimization, Tyler J. Free, Joseph P. Talley, Chad D. Hyer, Catherine J. Millar, Joel S. Griffitts, Bradley Charles Bundy May 2024

Engineering The Signal Resolution Of A Paper-Based Cell-Free Glutamine Biosensor With Genetic Engineering, Metabolic Engineering, And Process Optimization, Tyler J. Free, Joseph P. Talley, Chad D. Hyer, Catherine J. Millar, Joel S. Griffitts, Bradley Charles Bundy

Faculty Publications

Specialized cancer treatments have the potential to exploit glutamine dependence to increase patient survival rates. Glutamine diagnostics capable of tracking a patient’s response to treatment would enable a personalized treatment dosage to optimize the tradeoff between treatment success and dangerous side effects. Current clinical glutamine testing requires sophisticated and expensive lab-based tests, which are not broadly available on a frequent, individualized basis. To address the need for a low-cost, portable glutamine diagnostic, this work engineers a cell-free glutamine biosensor to overcome assay background and signal-to-noise limitations evident in previously reported studies. The findings from this work culminate in the development …

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Agroecology And Soil Stewardship: Values And Techniques Of Smallholder Farmers In Bernalillo County, Stephanie Olivas May 2024

Agroecology And Soil Stewardship: Values And Techniques Of Smallholder Farmers In Bernalillo County, Stephanie Olivas

Geography ETDs

Agrarian movements around the world use agroecology to build sovereignty and steward dynamic ecosystems. Research has shown that agroecological farmers steward more resilient crops, more resilient soil biomes, and greater biodiversity than conventional agriculture. GIS and remote sensing offer many tools to detect the impacts of these farmers on the environment, but it is less clear how such technologies fit into agroecological goals. This study asks: what values, experiences and knowledge do smallholder producers in Bernalillo County embody in their soil stewardship practices? Also, what experience or knowledge do smallholder producers in Bernalillo County have about remote sensing, and would …

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Reconstructing Hope And Resilience Among Kenyan Adolescent Immigrants, Julius Mwangi May 2024

Reconstructing Hope And Resilience Among Kenyan Adolescent Immigrants, Julius Mwangi

Doctor of Ministry Projects and Theses

The study "Reconstructing Hope and Resilience Among Kenyan Adolescent Immigrants" explores Kenyan immigrant families' challenges in the United States and their impact on their adolescent children. It proposes a community-led mentoring program incorporating emotional intelligence competencies and faith to support adolescents' success. Additionally, the study advocates for a new immigrant parent-mentoring program to help them navigate the complex legal process to accelerate their immigration and successful acculturalization. The research contains selected Bible narratives that identify parental responsibilities towards their children and discourses on their successful utility or lack thereof. The study argues that parents and the community have a responsibility …

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Supporting Science Identity Development With Course-Based Interventions: Strategies To Improve Undergraduate Student Persistence In Stem, Mariah Maxwell May 2024

Supporting Science Identity Development With Course-Based Interventions: Strategies To Improve Undergraduate Student Persistence In Stem, Mariah Maxwell

Dissertations - ALL

Science identity can be described as the part of oneself that relates to science, and is made up of four components: competence, performance, recognition, and interest. Science identity is important because students who have a strong science identity tend to persist longer in STEM majors and are more likely to attain a scientific career post-graduation than students with a weak science identity. Despite its importance for undergraduate persistence and career attainment, many students struggle to identify as a scientist, especially those who belong to groups that have historically been underserved in STEM. Therefore, exploring the ability of course-based interventions to …

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Women's Wings In Rebel Groups, Heidi Marie Stallman May 2024

Women's Wings In Rebel Groups, Heidi Marie Stallman

Dissertations - ALL

The proceeding dissertation is a collection of three articles exploring the phenomenon of women’s wings in rebel groups. The articles ask different questions and utilize diverse methodological tools. The three abstracts are below. Paper 1 exposes the patterns of women’s wings in rebel groups through a large-N analysis of 372 rebel groups existing between 1946 and 2015. Scholarship in rebel governance has begun to identify trends of how women organize and participate in rebel groups. Patterns of women’s participation in conflict vary widely in scope, purpose, and form between, within, and after conflict. This paper focuses on patterns of women’s …

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Investigating Vocal Development In Humpback Whales: Caller Identification, Acoustic And Movement Behavior, And Comparisons Across Age, Julia Marie Zeh May 2024

Investigating Vocal Development In Humpback Whales: Caller Identification, Acoustic And Movement Behavior, And Comparisons Across Age, Julia Marie Zeh

Dissertations - ALL

Studying variation in individual behavior can elucidate drivers related to factors like age, social context, and the environment. Differences in behavior with age are a product of survival needs and developmental differences as animals physiologically mature and modify their behaviors in response to experience. Learning is of particular interest across taxa because it is fundamental to the development of complex behaviors, from problem-solving to social hierarchies to animal songs and human language. Humpback whales (Megaptera noveaeangliae) exhibit a variety of complex and culturally transmitted behaviors across their range, including a diverse acoustic repertoire. Despite decades of research on adult behavior, …

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Three Essays On Representation, Participation, And Conflict In Environmental Justice Councils, Graham Ambrose May 2024

Three Essays On Representation, Participation, And Conflict In Environmental Justice Councils, Graham Ambrose

Dissertations - ALL

In this dissertation, representation, participation, and conflict are examined in state-mandated environmental justice councils. A novel dataset is constructed from council meeting minutes to explore patterns across these key concepts at the individual-level, meeting-level, and council level over time. The first essay examines how meeting-level factors – such as participant diversity, attributes of the meeting, and context – associate with meeting-level participation measured as different types of communication. This essay builds on participation research in collaborative governance, exploring participation through the lens of communication. Leveraging multiple OLS regressions, associations between meeting-level factors and participation through communication are explored. The results …

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Representation Of People With Disabilities In Legislatures: A Proposed Model Of Approval Representation, Yohannes Takele Zewale May 2024

Representation Of People With Disabilities In Legislatures: A Proposed Model Of Approval Representation, Yohannes Takele Zewale

Dissertations - ALL

This research examines the effect of the right to legislative representation for people with disabilities in the fields of Political Science and Law through a combination of doctrinal analysis and interviews with 12 individuals with disabilities. These individuals are disability rights advocates, politicians, and leaders of disability organizations from Kenya, Uganda, Rwanda, Zimbabwe, and Egypt, which are the only jurisdictions that have constitutionally recognized descriptive representation of people with disabilities in their legislatures. The research investigates the legal issues and challenges that people with disabilities face in achieving descriptive representation in their legislatures. Additionally, the research identifies gaps and deficiencies …

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Fabrication And Characterization Of 2d And 3d Shape-Memory Actuated Silk Wrinkled Surface Topographies For Biomedical Applications, Elizabeth Oguntade May 2024

Fabrication And Characterization Of 2d And 3d Shape-Memory Actuated Silk Wrinkled Surface Topographies For Biomedical Applications, Elizabeth Oguntade

Dissertations - ALL

Although biomaterials surface modification and smart materials science are heavily studied areas, the interface of the two has received substantially less investigation. Without this fundamental understanding, progress in biomaterials science at the interface of cells and dynamic functional materials will be hindered, preventing innovation in diverse biomedical applications. Thus, the goal of this dissertation is to acquire a fundamental understanding on the dynamic effects of shape-memory polymer (SMP) activation on biopolymer surface coatings, and to explore the potential biomedical application of this novel, material platform. To better understand the synergistic effects of surface modification and dynamic, shape memory activation, the …

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Climate Justice Before The Anthropocene: How Inclement Weather Shaped British And Irish Romanticism, Lauren Cooper May 2024

Climate Justice Before The Anthropocene: How Inclement Weather Shaped British And Irish Romanticism, Lauren Cooper

Dissertations - ALL

Climate Justice Before the Anthropocene examines British and Irish Romantic literature through the lens of climate. The tail end of the Little Ice Age and two volcanic eruptions halfway around the world contributed to severe and unpredictable climate patterns in the British Isles between 1790 and 1820 that exacerbated existing social inequities and economic distresses. In poems and novels that engaged with this extended climate crisis, the project argues, we encounter an emergent discourse of environmental justice that undercuts the celebrations of “nature,” the advocacy of human and animal rights, and the defenses of art that scholars so often identify …

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Geometric-Templated Cardiac Organoids For Drug Developmental Toxicity Screening, Shiyang Sun May 2024

Geometric-Templated Cardiac Organoids For Drug Developmental Toxicity Screening, Shiyang Sun

Dissertations - ALL

Nowadays 9 of every 10 women take at least one medicine during their pregnancy. However, unfortunately, many pregnant women have to face a severe problem that there is not enough evidence to prove the safety of their medication, and an appropriate medication might cause birth defects or even infant death. Researchers need an accurate and reliable drug developmental platform. Compared to the traditional animal and cell models, organoids have the ability to highly mimic the human organ developmental process, which allow researchers investigate the developmental toxicity to human development with organoid platform. The goal of this work is to explore …

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The Use Of Instructional Materials In Elementary Science Classrooms, Mary Jo Hoeft May 2024

The Use Of Instructional Materials In Elementary Science Classrooms, Mary Jo Hoeft

Dissertations - ALL

This mixed methods study explored how elementary teachers reported using a commercially published instructional unit to plan and deliver science instruction in their elementary classrooms. Of particular focus is what elements of the program teachers eliminated during planning and instruction, what outside materials teachers added to instruction, what modifications they made to the materials, and their rationales for these changes to the prescribed program. This study consisted of two phases. The first was a survey of elementary science teachers who taught the Smithsonian Science for the Classroom units. Data collected from this phase informed the subsequent interview phase. I used …

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Turbulent Landscapes: Reading The Borders Of Contemporary Latinx And Indigenous Literatures, Florencia Lauria May 2024

Turbulent Landscapes: Reading The Borders Of Contemporary Latinx And Indigenous Literatures, Florencia Lauria

Dissertations - ALL

Engaging with contemporary Latinx and Indigenous border literatures, films, and other cultural productions, my dissertation considers the tensions between Latinx migration and Indigenous sovereignty, claiming that their uneasy relation demands sustained critical and political attention. While Latinx cross-border migration and Indigenous peoples’ sovereignty struggles share important connections in the context of a colonial racial capitalist system of oppression, they cannot be easily collapsed into each other. Expanding upon Marisol de la Cadena’s concept of “partial connection,” my dissertation counters theoretical discourses that insist on commonality as the basis for Indigenous and Latinx intragroup solidarities. Moving beyond Gloria Anzaldúa’s borderlands concept …

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“But Is It Worth The Maintenance?”: Exploring The Ways Adolescent Preservice Teachers Develop Teaching Identities And Implement Pedagogical Practices, Tiffany Hamm May 2024

“But Is It Worth The Maintenance?”: Exploring The Ways Adolescent Preservice Teachers Develop Teaching Identities And Implement Pedagogical Practices, Tiffany Hamm

Dissertations - ALL

The climate crisis is an environmental issue that affects people of all walks of life but has disproportionate effects on those living in impoverished communities. The climate crisis therefore is not only an issue of environmental justice, but also social justice. However, incorporating environmental issues within education has typically been left to science classrooms. Teaching for environmental social justice, however, has the potential to extend beyond science content and science classrooms, and instead be incorporated in content across all academic disciplines. This dissertation is a teaching experiment that explored the ways adolescent preservice teachers from various disciplines develop their teaching …

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An Analysis Of The Musical Gestures In Osvaldo Golijov’S The Dreams And Prayers Of Isaac The Blind And Their Relationship To The Kabbalah Sefirot, Jeremy Gdaniec May 2024

An Analysis Of The Musical Gestures In Osvaldo Golijov’S The Dreams And Prayers Of Isaac The Blind And Their Relationship To The Kabbalah Sefirot, Jeremy Gdaniec

LSU Doctoral Dissertations

Osvlado Golijov’s The Dreams and Prayers of Isaac the Blind, scored for klezmer clarinet and string quartet, remains one of his most influential works since its premier in 1994. Inspiration for the work came from the writings of Rabbi Isaac the Blind, a prominent 13th century figurehead in the branch of Jewish mysticism known as Kabbalah. In Kabbalah, symbols known as the sefirot are emanations of creation and the key to unlocking the divine secrets of the God-head. The analysis presented in this document combines musical gesture theory with Kabbalistic concepts for new perspectives on Golijov’s …

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Molecular Dynamics Insights Into The Structural Behavior And Interactions Of Complex Biological Systems, Shenghan Song May 2024

Molecular Dynamics Insights Into The Structural Behavior And Interactions Of Complex Biological Systems, Shenghan Song

Chemistry and Chemical Biology ETDs

All-atom molecular dynamics (MD) simulations are essential for examining the structural and dynamic aspects of proteins at high resolution, complementing experiments limited by resolution, size, and complexity. Advancements in algorithms and hardware have enhanced the role of computational tools in scientific research, allowing for more accurate simulations of proteins with standard residues. However, simulating real biological systems, which include non-standard residues such as lipid membranes, remains challenging despite the assistance of tools like CHARMM-GUI. My research initially focused on simulating the PICK1 BAR dimer using steered MD simulations to investigate its structure and dynamics. Simulation works expanded to include systems …

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Directional Dopant Migration By ‘Atomic Trapping’ In Core/Multi-Shell Quantum Dots, Chun Chu May 2024

Directional Dopant Migration By ‘Atomic Trapping’ In Core/Multi-Shell Quantum Dots, Chun Chu

Dissertations - ALL

The study of transition metal ion-doped semiconductor nanocrystals (NCs) has attracted increasing attention due to their unique optical, electronic, and magnetic properties which sparked considerable interest towards a wide range of applications in material science, renewable energy and biological systems. Besides the size- and shape-dependent behavior, doped nanomaterials exhibit new and/or enhanced optical properties, which are sensitive to the dopant location or dopant distribution throughout the solid crystal lattice. Our work primarily focuses on understanding and elucidating the mechanism of dopant ion migration inside the NCs during post-synthetic treatments, such as shell passivation and high-temperature annealing. Mn(II) dopants migration behavior …

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Curing Bodies With Bodies: Renyao (Human-Derived Drugs) In Late Imperial China, Erqi Cheng May 2024

Curing Bodies With Bodies: Renyao (Human-Derived Drugs) In Late Imperial China, Erqi Cheng

Dissertations - ALL

This dissertation explores the philosophical and socioeconomic context of late imperial Chinese pharmacology and medical therapies from the sixteenth to the eighteenth centuries. It examines the rise of human-derived drugs (renyao) in the sixteenth century and their subsequent decline in the late seventeenth and eighteenth centuries, influenced by a complex interplay of changes in social mores, urban tastes, commercial publishing, and philosophical ideologies. By drawing from a diverse range of sources including bencao (materia medica) works, formularies, Daoist texts, government documents, biji (belles lettres), novels, collected verses, and local gazetteers, the dissertation argues that Chinese medical culture in the Ming …

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Evaluating The Clustering Behavior Of Antimicrobial Peptoids For Application In Implantable Medical Devices, Anand Sunil Wadurkar May 2024

Evaluating The Clustering Behavior Of Antimicrobial Peptoids For Application In Implantable Medical Devices, Anand Sunil Wadurkar

Theses - ALL

Treating biofilm infections on medical implants is a significant healthcare challenge, often requiring device removal and potentially leading to amputations. This study investigates the efficacy of biocompatible peptoids as a novel strategy to combat these infections. This study employs atomistic simulations and small-angle X-ray scattering to investigate the self-assembly behavior of four peptoid sequences (TM1, TM4, TM6, TM22). Peptoids forming supramolecular assemblies display enhanced complexation with polyanionic microgels, promising for antimicrobial coatings on implantable devices. Atomistic simulations and experimental data analysis reveal a strong link between peptoid sequence, hydrophobicity, and propensity for self-assembly. The highly hydrophobic TM4 forms large multimeric …

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Revelations Of Mary Shelley: Death, Immortality, And The Creator-Creature Relationship In Frankenstein, Yuqing Liu May 2024

Revelations Of Mary Shelley: Death, Immortality, And The Creator-Creature Relationship In Frankenstein, Yuqing Liu

Theses - ALL

This thesis aims to explore the cultural, and societal backgrounds, and interpersonal relationships that influenced Mary Shelley in crafting Frankenstein, and to understand how these elements are mirrored in the novel. It endeavors to examine the linkage between the intentions that authors embed within their works and the realm of reality. Analyzing Shelley's inspirations and motives through the prism of her social context and personal connections, this thesis delves into the Romantic and Gothic literary traits manifested in Frankenstein. Through this inquiry, a creative work inspired by Frankenstein was developed, intending to serve as a vessel for reflection on philosophical …

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Empowerment Or Entrapment? A Critical Analysis Of Microfinance Operations Among Female Borrowers In Kitui County, Kenya, Eric Kioko Musembi May 2024

Empowerment Or Entrapment? A Critical Analysis Of Microfinance Operations Among Female Borrowers In Kitui County, Kenya, Eric Kioko Musembi

Theses - ALL

“Empowerment or Entrapment? A Critical Analysis of Microfinance Operations among Female Borrowers in Kitui County, Kenya” is a research project that aims to understand the dynamics of microfinance loan expectations and their implications for women. For this study, one cannot help but borrow or get insights from those who have written about the culture of Africa. To a greater extent, they all touch on the anthropological aspect of Africa. I use anthropological/ cultural theoretical perspectives, political economy, and Afro-feminism as my leading theories. I hypothesize that where women are typically engaged in heavy domestic chores and are undervalued and under-compensated …

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Mental Health Problems Of The Firstborn Children In China, Jianye Zou May 2024

Mental Health Problems Of The Firstborn Children In China, Jianye Zou

Theses - ALL

The aim of this thesis is to investigate the negative psychological impact that first-born children experience when facing the impending arrival of younger siblings in their families and the factors of these problems in China. In addition, this thesis helps the children prepare for the process of fitting in the new family structure via relevant artworks. Based on the unique situation, such as the only-child policy and culture of Chinese society, this thesis analyzes the factors affecting first-born children during the transition from being an only child to having siblings during the arrival of a second child within the family. …

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Public Lands And Capitalism: The Case Of The Adirondack Park, Weston (Trent) Fenner May 2024

Public Lands And Capitalism: The Case Of The Adirondack Park, Weston (Trent) Fenner

Dissertations - ALL

This dissertation examines the tensions that surround land management in the Adirondack Park of New York State (NYS), while paying special attention to how the market intrudes into these conflicts. To look at these tensions, this project explores contests over the regulation of public and private land, conflicts over how wilderness is imagined and managed, and the controversies that flow from efforts to alleviate high-use problems in the Adirondack Park. These contests are the basis of the following research questions which reveal the impact of land management conflict in the Adirondack Park, and expose how the market influences park management …

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Twice As Good: A Constructivist Grounded Theory Study On The Socialization Process Of Black Middle-Class Families And How This Shapes The Parent-Child Relationship, Dominique Nicole Walker May 2024

Twice As Good: A Constructivist Grounded Theory Study On The Socialization Process Of Black Middle-Class Families And How This Shapes The Parent-Child Relationship, Dominique Nicole Walker

Dissertations - ALL

The purpose of this study was to describe and explain the socialization process of Black middle-class families and explore how this shapes the parent-child relationship. The methodology that was used for this study was constructivist grounded theory. Also included within this methodology were theories and frameworks such as: Narrative Family therapy, contextual therapy, and the Intersectionality framework. Using semi-structured interviews, participants were asked to reflect upon their middle-class upbringing and to share how their socialization process shaped their relationship with their parents, both in the past and currently. There was an array of themes that were discovered from the results. …

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Multi-Scale Analysis Of The Structure-Mechanics Relationship Of Mycelium-Based Bio-Composites, Libin Yang May 2024

Multi-Scale Analysis Of The Structure-Mechanics Relationship Of Mycelium-Based Bio-Composites, Libin Yang

Dissertations - ALL

The construction industry faces significant challenges due to the environmental impact of traditional materials like steel and concrete, whose production is energy-intensive and contributes to environmental pollution. This issue is exacerbated by the growing global demand for construction materials, driven by an increasing population. Concurrently, the agricultural and wood industrial sectors produce vast byproducts, such as straw, husk, coir, sawdust, and wood chips. They are often considered as wastes and disposed of through methods like burning. Such a reckless treatment releases greenhouse gases that further lead to environmental degradation. Mycelium, the vegetative part of fungi, presents a natural, promising solution …

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“Rude, Misshapen, Monstrous Rabblement”: Race, Disability, And Gender In Early Modern England, Deyasini Dasgupta May 2024

“Rude, Misshapen, Monstrous Rabblement”: Race, Disability, And Gender In Early Modern England, Deyasini Dasgupta

Dissertations - ALL

My first chapter, “‘Ugly Monstrous Shapes’: Reading Signs of Alterity,” looks at presentations of “othered” female bodies in Book I of Edmund Spenser’s The Faerie Queene. This chapter draws our attention to various old, deformed, or otherwise dysfunctional bodies. In doing so, it not only emphasizes the necessity for reading but also listening to the “ugly monstrous shapes, that elsewhere no man may” find. Drawing attention to metaphor, rhyme, and non-verbal sounds produced by the disabled/disabling bodies in the poem, this chapter locates Spenser’s racialization of bodies in his careful cultivation of sympathy. By using sound as a disability structure, …

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Fate, Composition And Formation Of Unknown N-Nitrosamines In Wastewater And Wastewater-Impacted Waters, Changcheng Pu May 2024

Fate, Composition And Formation Of Unknown N-Nitrosamines In Wastewater And Wastewater-Impacted Waters, Changcheng Pu

Dissertations - ALL

N-Nitrosamines form as byproducts during oxidative water treatment or occur as impurities in consumer and industrial products. N-Nitrosamines and their precursors pose significant concerns for water utilities exploiting wastewater-impacted water supplies, as many N-nitrosamines are potential human carcinogens and have genotoxicity. To date, research on N-nitrosamines in environmental engineering has primarily focused on the occurrence, sources, formation, and control of N-nitrosodimethylamine (NDMA) in the broad context of drinking water and wastewater treatment. However, NDMA and the few other N-nitrosamines of current interest only constitute a fraction of the larger pool of total N-nitrosamines (TONO) in source waters and wastewater, while …

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From Haiti To Durban: Repairing The Crime Against Humanity, Aimee Beatrice Shukuru May 2024

From Haiti To Durban: Repairing The Crime Against Humanity, Aimee Beatrice Shukuru

Theses - ALL

Black people have been at the center of the liberation struggle since the transatlantic slave trade that saw black people as property. The various forms of resistance within the struggle have taken many shapes, from jumping off the slave ships in the Atlantic to breaking tools and refusing to work to outright organized resistance that led to a revolution in Haiti that defeated slavery, colonization, and white supremacy that made capital accumulation based on the dehumanization of black people possible. Because the transatlantic slave trade was a global project, black people have demanded nothing short of an international recognition of …

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Under The Skin Of Us: The Unfailing Narrative Potential Of Beauty And The Beast, Xiaoyue Lu May 2024

Under The Skin Of Us: The Unfailing Narrative Potential Of Beauty And The Beast, Xiaoyue Lu

Theses - ALL

This thesis represents an initial exploration into the reasons behind the enduring creative vitality of the Beauty and the Beast motif among creators. It offers a forward-looking perspective on the untapped narrative potential of this motif, based on an analysis of the evolution of character archetypes and derivative works, primarily in film, over time. The thesis begins by tracing the evolution of Beauty and the Beast from its earliest fairy tales to subsequent major film adaptations. It then examines why Belle and the Beast, as characters, have continued to captivate audiences and readers across changing eras, setting them apart from …

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