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Targeting Trpc3: A Promising Therapeutic Strategy For Alzheimer's Disease, Jiaxing Wang

Alternative Theses and Dissertations (AETDs)

Alzheimer's disease (AD) is a progressive neurological disorder characterized by extracellular β-amyloid (Aβ) plaques and intracellular tau tangles. The calcium (Ca2+) hypothesis of AD proposes that disrupted intracellular Ca2+ regulation underlies AD pathology, contributing to inflammation, oxidative stress, impaired autophagy, neurodegeneration, and cognitive dysfunction. Store-operated Ca2+ entry (SOCE) maintains ER Ca2+ stores, involving transient receptor potential canonical (TRPC) and Orai channels. Specifically, we speculated that dysregulated functions of TRPC3 contribute to the pathogenesis of AD. We believe that targeting TRPC3 with a pharmacologically feasible agent could prove beneficial in halting cognitive decline. We previously reported compound JW-65, a novel, metabolically …

Animal Science Research Report 2024 (Complete Report), Department Of Animal Science

Animal Science Research Report 2024

This is the complete proceedings of the South Dakota State University, Department of Animal Science Research Report 2024. The report contains research on ruminant, non-ruminant, and meat science.

The Effects Of Nano-Curcumin On Growth Performance, Feed Utilization, Blood Biochemistry, Disease Resistance, And Gene Expression In European Seabass (Dicentrarchus Labrax) Fingerlings, Samyah Justaniah, Ahd. A. Mansour, Ayat H. Al-Tarawni, Ahab El-Haroun, Mohhamed Bodrul Munir, Sultan Ayesh M. Saghir, Zulhisyam Abdul Kari, G. Tellez-Isaias, Walter G. Bottje, Ammar Mohammed Hamood Al-Farga, El-Sayed Hemdan Eissa

Poultry Science Faculty Publications and Presentations

Phytochemicals are used in fish farming to reduce stress and combat diseases during intensification. Recently, nanotechnology has represented a paradigm shift in the aquatic feed industry to improve the solubility, availability, and efficacy of phytochemicals. The purpose of this research was to investigate the effects of dietary supplementation with nano-curcumin (CUNE) on the growth, feed utilization, body composition, blood biochemistry, antioxidant status, disease resistance to Vibrio parahaemolyticus (V. parahaemolyticus), and the expression of insulin-like growth factor 1 (IGF-1), growth hormone (GH), interleukin-10 (IL-10), and interlukin-1β (IL-1β) genes in European Seabass (Dicentrarchus …

Identifying Dietary Timing Of Organic Trace Minerals To Reduce The Incidence Of Osteomyelitis Lameness In Broiler Chickens Using The Aerosol Transmission Model, Khawla Alharbi, Andi Asnayanti, Anh Dang Trieu Do, Ruvindu Perera, Layla Al-Mitib, Abdulkarim Shwani, Marco A. Rebollo, Michael T. Kidd, Adnan Ali Khalaf Alrubaye

Poultry Science Faculty Publications and Presentations

Our prior research demonstrated a 20% to 25% reduction in bacterial chondronecrosis with osteomyelitis (BCO) lameness in broilers with organic Zn, Mn, and Cu (Availa^® ZMC) supplementation. Expanding on this, we investigated the optimal timing for Availa^® ZMC feeding to mitigate BCO lameness and reduce feed additive costs in the poultry industry. In this study, we compared the application of 0.15% Availa^® ZMC for 56 days, the first 28 days, and the last 28 days. The experimental design was a randomized block design involving 1560 one-day-old chicks distributed across two wire-floor pens as BCO source infection and …

Gender Disparity In Students’ Performance Of Science And Mathematics: Evidence From Nationwide Study In Pakistan, Sadia Muzaffar Bhutta, Aisha Naz Ansari, Sohail Ahmad

Institute for Educational Development, Karachi

Gender disparity in science and mathematics performance is being researched internationally. In a complex education system, like Pakistan, studying the difference in isolation may not generate holistic understanding. Therefore, this paper investigates changes in trends of performance in Science and Mathematics for both genders across public and private schools. Data were gathered from a nationwide sample of elementary grades students (n=15,391) using SATs/MATS. Independent t-test and two-way-ANOVA were employed to analyse data. Results revealed that girls outperformed boys in Science (p<0.01) while no gender differences in performance were observed in Mathematics. Gender and school systems demonstrated significant interaction effect only in mathematics, unveiling that girls perform better in public schools while this difference diminished in the private school system. The results show implications for employing gender-fair policies and practices in science and mathematics education.

Lucid Dream: Embracing Uncertainty, Zhiyun Zhang

Masters Theses

Glass is a tool, a projection surface, a medium for optical phenomena. It creates a tangible link between physical space and perception, transcending the physical realm to reshape our sensory perceptions and resonate within our psyche, deepening our understanding. Inspired by cosmic and natural phenomena, my installations narrate the contemplation of the moon and the appreciation of rainbows. They reference historic optical technologies, incorporating glass, mirrors, light, darkness, and space to produce an encounter that extends beyond personal experience, resonating with our collective unconscious, uniting us in profound ways. The blurred boundaries between observer and observed, reality and illusion, sensory …

Transitioning To Regenerative Agriculture One French Fry At A Time, Alexia Brunet Marks

Nevada Law Journal

No abstract provided.

Bridging The Divide: Does The Eu's Ai Act Offer Code For Regulating Emergent Technologies In America?, Renee Henson

Missouri Law Review

The European Union (“EU”) has taken the remarkable step of coming to agreement to implement the first-of-its-kind comprehensive legislation regarding artificial intelligence (“AI”), the AI Act. The AI Act adopts a risk-based approach to address diverse AI system applications and potential harms associated with AI technology. The AI Act categorizes AI systems based on risk levels, ranging from unacceptable to minimal, with corresponding regulatory requirements tailored to mitigate associated risks. Efforts are underway in the United States to establish AI regulatory frameworks, as demonstrated by the Bipartisan Framework for U.S. Act, proposed by Senators Josh Hawley (R-MO) and Richard Blumenthal …

Loose Canons: The Supreme Court's New Interpretive Methodology, Jacob Wood

Missouri Law Review

Just over fifty years ago, Congress enacted the Clean Water Act (“CWA”) to address burning rivers and bacteria infested waterways that plagued the United States. The objective of the Act was to “restore and maintain the chemical, physical, and biological integrity of the Nation’s waters.” The judicial branch is responsible for reviewing and interpreting the law, and through such review, the United States Supreme Court has been tasked with interpreting the CWA more than any piece of environmental legislation. In Sackett v. EPA, it did so using a new interpretive approach: canonism.

Using Intellectual Property To Regulate Artificial Intelligence, Dennis D. Crouch

Missouri Law Review

This Article examines the complex relationship between intellectual property (“IP”) rights and the regulation of artificial intelligence (“AI”). It advances two primary claims: First, while IP plays a role in guiding innovative behaviors in AI development, it does not serve as an effective mechanism for direct regulation of AI. This claim is based on the observation that IP rights, such as patents and copyrights, are primarily designed to incentivize innovation and protect creative works, while lacking the levers necessary to address the broader societal implications of AI technology. The narrow focus of IP rights on rewarding creators makes them ill-suited …

Artificial Intelligence And Antibody Genus Claims, Thomas R. Langdon

Missouri Law Review

Antibodies are the guard dogs of the human immune system. They travel through the bloodstream, sniffing out foreign invaders (antigens), binding to them, and preventing them from harming the body. Instead of having a nose, four legs, and a tail, antibodies are Y-shaped proteins comprised of amino acids that viciously protect their hosts. Think of the tips of the “Y” as mouths that can bite certain antigens and lock them in place, rendering them harmless. Antibodies have the ability to identify a plethora of antigens to bind to and neutralize; “[s]ome researchers have estimated that the theoretical number of different …

Radiogenomics-Based Risk Prediction Of Glioblastoma Multiforme With Clinical Relevance, Xiaohua Qian, Hua Tan, Xiaona Liu, Weiling Zhao, Michael D Chan, Pora Kim, Xiaobo Zhou

Faculty, Staff and Student Publications

Glioblastoma multiforme (GBM)is the most common and aggressive primary brain tumor. Although temozolomide (TMZ)-based radiochemotherapy improves overall GBM patients' survival, it also increases the frequency of false positive post-treatment magnetic resonance imaging (MRI) assessments for tumor progression. Pseudo-progression (PsP) is a treatment-related reaction with an increased contrast-enhancing lesion size at the tumor site or resection margins miming tumor recurrence on MRI. The accurate and reliable prognostication of GBM progression is urgently needed in the clinical management of GBM patients. Clinical data analysis indicates that the patients with PsP had superior overall and progression-free survival rates. In this study, we aimed …

Neuropsychological Intra-Individual Variability In Traumatic Brain Injury: Clinical Utility, Classification, And Associations With Outcomes, Jeremy A. Feiger

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Traumatic brain injury (TBI) survivors experience a range of sequelae including neurobiological brain changes, cognitive impairment, psychological difficulties, and functional changes. Neuropsychological assessment after injury is used to characterize current strengths and weaknesses to inform prognosis and interventions. There is currently no reliable and consistent method of assessing and classifying injuries in the post-acute and chronic phases of moderate to severe TBI (msTBI) that is associated with long term psychosocial outcomes. This study aimed to combine the use of within-person variability on neuropsychological test batteries and in cognitive response latency, known as intra-individual variability (IIV), with a novel cognitive phenotyping …

Thermochromic Yarns’ Application In Fever Detection For Young Children, Sibei Xia, Chuanlan Liu, Yanbo Zhang, Bruce Cameron

Faculty Publications

The existing literature emphasizes the significance of and needs for developing body temperature monitoring devices that can consistently and discreetly assess the temperatures of young children. Such products could offer a method to check children’s body temperature and alleviate parents’ concerns over fever. However, research gaps and challenges exist in preserving material flexibility, conducting tests in a controlled setting that mimics body temperature, and investigating consumer perceptions of this type of functional textile product. Therefore, this research aimed to investigate functional textiles using thermochromic yarns for young children’s body temperature detection, particularly focusing on testing prototypes in a controlled environment …

Male Song Structure Predicts Offspring Recruitment To The Breeding Population In A Migratory Bird, Rachael A. Disciullo, Anna M. Forsman, Robert R. Fitak, John Hunt, Pirmin Nietlisbach, Charles F. Thompson, Scott K. Sakaluk

Faculty Publications – Biological Sciences

Bird song is a classic example of a sexually selected trait, but much of the work relating individual song components to fitness has not accounted for song typically being composed of multiple, often-correlated components, necessitating a multivariate approach. We explored the role of sexual selection in shaping the complex male song of house wrens (Troglodytes aedon) by simultaneously relating its multiple components to fitness using multivariate selection analysis, which is widely used in insect and anuran studies but not in birds. The analysis revealed significant variation in the form and strength of selection acting on song across different …

A Case Of Charcot-Marie-Tooth Disease Type 2o (Cmt 20) Associated With Dync1h1 And Slc12a6 Mutations, Tehreem Arshad, Muhammad Jawad Hassan, Arsalan Ahmad

Pakistan Journal of Neurological Sciences (PJNS)

Charcot-Marie-Tooth disease (Charcot Marie-Tooth) comprises of a variety of hereditary diseases affecting the peripheral nervous system. Charcot Marie-Tooth2 is a subgroup caused by defects in the axon of the peripheral nerve cells. Charcot Marie-Tooth Type 2O is an uncommon type that affects less than one in a million people and is linked to specific genetic abnormalities. We report a case study of a 66-year-old individual with Charcot Marie-Tooth Type 2O, diagnosed on clinical presentation and identified through genetic analysis. Electromyography and nerve conduction studies supported the diagnosis of an axonal sensorineural neuropathy. A comprehensive genetic neuropathies panel led to the …

A Living Storyscape, Vivian Combariza

Masters Theses

Advocating for a transformative shift, the following proposes a new cultural setting that fosters diverse voices and dialogue, moving away from the singular narratives imposed by traditional curator-visitor dynamics. By drawing inspiration from nature's complexity and mirroring museums with ecosystems, the aim of this thesis is to emulate the adaptability and interconnectedness of natural ecosystems to develop flexible and inclusive environments, encouraging community engagement and shared learning experiences. This new design paradigm fosters a deeper connection with nature and other beings despite the physical separation. Embracing the collaboration and flexibility of nature, cultural institutions can regain relevance as vibrant centers …

Impact Of Low-Load Resistance Exercise With And Without Blood Flow Restriction On Muscle Strength, Endurance, And Oxidative Capacity: A Pilot Study, Brett H. Davis, James E. Stampley, Joshua Granger, Matthew C. Scott, Timothy D. Allerton, Neil M. Johannsen, Guillaume Spielmann, Brian A. Irving

Clinical Research Faculty Publications

Low-load resistance exercise (LLRE) to failure can increase muscle mass, strength, endurance, and mitochondrial oxidative capacity (OXPHOS). However, the impact of adding blood flow restriction to low-load resistance exercise (LLBFR) when matched for volume on these outcomes is incompletely understood. This pilot study examined the impact of 6 weeks of single-legged LLBFR and volume-matched LLRE on thigh bone-free lean mass, strength, endurance, and mitochondrial OXPHOS. Twenty (12 males and 8 females) untrained young adults (mean ± SD; 21 ± 2 years, 168 ± 11 cm, 68 ± 12 kg) completed 6 weeks of either single-legged LLBFR or volume-matched LLRE. Participants …

Echoes Of Nature: Using Biodata Sonification To Model Electrical Communication In Plants, Penelope Anna Alioshin

Electrical Engineering

The use of biodata sonification as a tool to visualize biological data is a response to recent developments in the intersection of art and technology. This project explores the intersection by converting the electrical impulses given off by plants into audio signals. By processing and amplifying the voltage difference between two electrodes on a plant, its communication is quantified. The goal is to use this communication model as a tool to increase ecological awareness as well as improve machine learning models. A deeper understanding of non-human communication systems, specifically those that employ superposition decision matrices, could help to drastically increase …

Matrix Approximation And Image Compression, Isabella R. Padavana

Master's Theses

This thesis concerns the mathematics and application of various methods for approximating matrices, with a particular eye towards the role that such methods play in image compression. An image is stored as a matrix of values with each entry containing a value recording the intensity of a corresponding pixel, so image compression is essentially equivalent to matrix approximation. First, we look at the singular value decomposition, one of the central tools for analyzing a matrix. We show that, in a sense, the singular value decomposition is the best low-rank approximation of any matrix. However, the singular value decomposition has some …

Parent Strategies For Antiracist Parenting In Families Of Multiracial And Asian Descent, Matlida Tavares

Doctoral Dissertations

The Pew Research Center’s analysis of United States census data depicts and increasing number of individuals identifying as more than one race. From 2010 to 2020, the population had nearly doubled to 13.5 million. This is particularly evident in states with large populations of Asian American communities such as California, where nearly two million people identify as multiracial and people identifying as both White and Asian making up the largest portion of the 61% increase the state saw since 2010 (Henderson, 2022). Despite claims that an increase in multiracial individuals signifies an end of racism, multiracial children, navigating complex ethnic …

Event-Free Survival Of Maralixibat-Treated Patients With Alagille Syndrome Compared To A Real-World Cohort From Gala., Bettina E. Hansen, Shannon M. Vandriel, Pamela Vig, Will Garner, Douglas B. Mogul, Kathleen M. Loomes, David A. Piccoli, Elizabeth B. Rand, Irena Jankowska, Piotr Czubkowski, Dorota Gliwicz-Miedzińska, Emmanuel M. Gonzales, Emmanuel Jacquemin, Jérôme Bouligand, Lorenzo D'Antiga, Emanuele Nicastro, Henrik Arnell, Björn Fischler, Étienne Sokal, Tanguy Demaret, Susan Siew, Michael Stormon, Saul J. Karpen, Rene Romero, Noelle H. Ebel, Jeffrey A. Feinstein, Amin J. Roberts, Helen M. Evans, Shikha S. Sundaram, Alexander Chaidez, Winita Hardikar, Sahana Shankar, Ryan T. Fischer, Florence Lacaille, Dominique Debray, Henry C. Lin, M Kyle Jensen, Catalina Jaramillo, Palaniswamy Karthikeyan, Giuseppe Indolfi, Henkjan J. Verkade, Catherine Larson-Nath, Ruben E. Quiros-Tejeira, Pamela L. Valentino, Maria Rogalidou, Antal Dezsőfi, James E. Squires, Kathleen Schwarz, Pier Luigi Calvo, Jesus Quintero Bernabeu, Andréanne N Zizzo, Gabriella Nebbia, Pinar Bulut, Ermelinda Santos-Silva, Rima Fawaz, Silvia Nastasio, Wikrom Karnsakul, María Legarda Tamara, Cristina Molera Busoms, Deirdre A. Kelly, Thomas Damgaard Sandahl, Carolina Jimenez-Rivera, Jesus M. Banales, Quais Mujawar, Li-Ting Li, Huiyu She, Jian-She Wang, Kyung Mo Kim, Seak Hee Oh, Maria Camila Sanchez, Maria Lorena Cavalieri, Way Seah Lee, Christina Hajinicolaou, Chatmanee Lertudomphonwanit, Orith Waisbourd-Zinman, Cigdem Arikan, Seema Alam, Elisa Carvalho, Melina Melere, John Eshun, Zerrin Önal, Dev M. Desai, Sabina Wiecek, Raquel Borges Pinto, Victorien M. Wolters, Jennifer Garcia, Marisa Beretta, Nanda Kerkar, Jernej Brecelj, Nathalie Rock, Eberhard Lurz, Niviann Blondet, Uzma Shah, Richard J. Thompson, Binita M. Kamath, Global Alagille Alliance (Gala) Study Group

Manuscripts, Articles, Book Chapters and Other Papers

BACKGROUND AND AIMS: Alagille syndrome (ALGS) is characterized by chronic cholestasis with associated pruritus and extrahepatic anomalies. Maralixibat, an ileal bile acid transporter inhibitor, is an approved pharmacologic therapy for cholestatic pruritus in ALGS. Since long-term placebo-controlled studies are not feasible or ethical in children with rare diseases, a novel approach was taken comparing 6-year outcomes from maralixibat trials with an aligned and harmonized natural history cohort from the G lobal AL agille A lliance (GALA) study.

APPROACH AND RESULTS: Maralixibat trials comprise 84 patients with ALGS with up to 6 years of treatment. GALA contains retrospective data from 1438 …

Sars-Cov-2 Envelope Protein Regulates Innate Immune Tolerance., Eric S. Geanes, Rebecca Mclennan, Stephen H. Pierce, Heather Menden, Oishi Paul, Venkatesh Sampath, Todd Bradley

Manuscripts, Articles, Book Chapters and Other Papers

Severe COVID-19 often leads to secondary infections and sepsis that contribute to long hospital stays and mortality. However, our understanding of the precise immune mechanisms driving severe complications after SARS-CoV-2 infection remains incompletely understood. Here, we provide evidence that the SARS-CoV-2 envelope (E) protein initiates innate immune inflammation, via toll-like receptor 2 signaling, and establishes a sustained state of innate immune tolerance following initial activation. Monocytes in this tolerant state exhibit reduced responsiveness to secondary stimuli, releasing lower levels of cytokines and chemokines. Mice exposed to E protein before secondary lipopolysaccharide challenge show diminished pro-inflammatory cytokine expression in the lung, …

A Delphi Panel To Build Consensus On Assessing Disease Severity And Disease Progression In Adult Patients With Hypophosphatasia In The United States., K M Dahir, Eric T. Rush, S Diaz-Mendoza, P S Kishnani

Manuscripts, Articles, Book Chapters and Other Papers

BACKGROUND: Hypophosphatasia (HPP) is an inborn error of metabolism with a variable presentation. We conducted a modified Delphi panel to obtain expert consensus on knowledge gaps regarding disease severity and progression in adult patients with HPP.

METHODS: Healthcare professionals (HCPs) with experience managing adult patients with HPP were recruited to participate in a 3-round Delphi panel (round 1: paper survey and 1:1 interview; rounds 2-3: email survey). Panelists rated the extent of their agreement with statements about disease severity and progression in adult patients with HPP. Consensus was defined as ≥ 80% agreement.

RESULTS: Ten HCPs completed round 1; nine …

Best Practice Of Peritoneal Dialysis-Associated Gram-Negative Peritonitis In Children: Insights From The International Pediatric Peritoneal Dialysis Network Registry., Dagmara Borzych-Dużałka, Rebeca Same, Alicia Neu, Hui Kim Yap, Enrico Verrina, Sevcan A Bakkaloglu, Francisco Cano, Hiren Patel, Maria Szczepańska, Łukasz Obrycki, Ana Paula Spizzirri, Lisa Sartz, Karel Vondrak, Anabella Rebori, Gordana Milosevski-Lomic, Eugene Yu-Hin Chan, Biswanath Basu, Andrea Lazcano Pezo, Ariane Zaloszyc, Vimal Chadha, Franz Schaefer, Bradley A. Warady

Manuscripts, Articles, Book Chapters and Other Papers

INTRODUCTION: Gram-negative peritonitis (GNP) is associated with significant morbidity in children receiving long-term peritoneal dialysis (PD) and current treatment recommendations are based on limited data.

METHODS: Analysis of 379 GNP episodes in 308 children (median age 6.9 years, interquartile range [IQR]: 3.0-13.6) from 45 centers in 28 countries reported to the International Pediatric Peritoneal Dialysis Network registry between 2011 and 2023.

RESULTS: Overall, 74% of episodes responded well to empiric therapy and full functional recovery (FFR) was achieved in 82% of cases. In vitro bacterial susceptibility to empiric antibiotics and lack of severe abdominal pain at onset were associated with …

Effects Of Food Supplementation And Helminth Removal On Space Use And Spatial Overlap In Wild Rodent Populations, Janine Mistrick, Jasmin S.M. Veitch, Shannon M. Kitchen, Samuel Clague, Brent C. Newman, Richard J. Hall, Sarah A. Budischak, Kristian M. Forbes, Megan E. Craft

Biological Sciences Faculty Publications and Presentations

• Animal space use and spatial overlap can have important consequences for population-level processes such as social interactions and pathogen transmission. Identifying how environmental variability and inter-individual variation affect spatial patterns and in turn influence interactions in animal populations is a priority for the study of animal behaviour and disease ecology. Environmental food availability and macroparasite infection are common drivers of variation, but there are few experimental studies investigating how they affect spatial patterns of wildlife.
• Bank voles (Clethrionomys glareolus) are a tractable study system to investigate spatial patterns of wildlife and are amenable to experimental manipulations. We conducted …

Optimizing Central Venous Access Simulation Training To Surgical Education Trends: A Hybrid Approach Of 3d Anatomy Technology And Flipped Classroom, Ung-Kai Ting, Ming-Hsien Chiang, Yung-Chih Wang, Chin-Sheng Lin, Jiunn-Tay Lee, Yuan-Ming Tsai

Journal of Medical Education

Purpose: Central venous catheterization is a critical skill in perioperative and critical care medicine. During their postgraduate-year training (PGY), postgraduate trainees are expected to perform central venous catheter (CVC) insertion. Despite learning anatomy in medical school, the demanding nature of the hospital environment requires continuous reinforcement of their knowledge. This pilot study examines the efficacy of three-dimensional (3D) anatomy technology in teaching vascular anatomy for CVC insertion through a flipped classroom approach. Methods: An educational 3D anatomy platform "Complete Anatomy" was utilized to create central-line vascular models to teach vascular anatomy. Trainees completed an online course and a self-assessment before …

Do Cognitive Resources Matter For Eudaimonia? The Role Of Inhibitory Control In Psychological Well-Being, Wei Xing Toh, Hwajin Yang

Research Collection School of Social Sciences

Although past work has investigated the psychosocial and biological correlates of psychological well-being (PWB), little is known regarding the cognitive underpinnings of PWB. Given that prior research has predominantly relied on indices of general cognitive abilities and PWB, we examined the relations between Ryff’s (1989) six-factor model of PWB and inhibitory control (i.e. the ability to actively maintain pertinent goals by resisting interference from irrelevant information or prepotent responses). Using structural equation modelling (N = 170), based on three inhibition measures and six facets of PWB, we found that better inhibitory control was associated with greater personal growth, when cognitive, …

Subjective Socioeconomic Status Moderates How Resting Heart Rate Variability Predicts Pain Response, Jacinth Jia Xin Tan, Chin Hong Tan, Michael W. Kraus

Research Collection School of Social Sciences

Higher resting heart rate variability (HRV)—an index of more flexible response to environmental stressors, including noxious stimuli—has been linked to reduced perception of experimentally induced pain. However, as stress responses are adapted to one’s chronic environments, we propose that chronic exposure to threats captured by one’s subjective socioeconomic status (SSS) may shape different adaptations that produce distinct pain responses linked to higher resting HRV. Specifically, lower SSS individuals with more threat exposures may prioritize threat detection by upregulating sensitivity to stressors, such as acute pain. Therefore, higher HRV would predict greater perceived acute pain among lower SSS individuals. In contrast, …

Scaffold-Free Development Of Multicellular Tumor Spheroids With Spatial Characterization Of Structure And Metabolic Radial Profiles, Shelby N. Bess, Gaven K. Smart, Matthew J. Igoe, Timothy J. Muldoon

Biomedical Engineering Faculty Publications and Presentations

Purpose

In vitro assays are essential for studying cellular biology, but traditional monolayer cultures fail to replicate the complex three-dimensional (3D) interactions of cells in living organisms. 3D culture systems offer a more accurate reflection of the cellular microenvironment. However, 3D cultures require robust and unique methods of characterization.

Methods

The goal of this study was to create a 3D spheroid model using cancer cells and macrophages, and to demonstrate a custom image analysis program to assess structural and metabolic changes across spheroid microregions.

Results

Structural characterization shows that cells at the necrotic core show high normalized fluorescence intensities of …