Open Access. Powered by Scholars. Published by Universities.®

Digital Commons Network™

Open Access. Powered by Scholars. Published by Universities.®

Discipline
Institution
Keyword
Publication Year
Publication
Publication Type
File Type

Articles 71941 - 71970 of 820923

Full-Text Articles in Entire DC Network

An Efficient Method For Recognition Of Human-In-Motion Action Based On Foot-Lift Features, Khin Cho Tuna, Hla Myo Tunb Jun 2024

An Efficient Method For Recognition Of Human-In-Motion Action Based On Foot-Lift Features, Khin Cho Tuna, Hla Myo Tunb

ASEAN Journal on Science and Technology for Development

In the age of Industry 4.0, recognition of human moving actions becomes an essential element in smart surveillance systems at public places. The requirement of a large number of frames, view dependency and the requirement of big database are still challenging issues for human action recognition in real-time applications. This study proposes an efficient method for basic moving actions, “Walking” and “Running”, based on foot-lift features aiming at the recognition of ongoing moving action by reducing the number of frames required. The plane of moving path and foot-lift are estimated during the action by means of BLOB analysis. The performance …

Go to article

Strikingly Attractive: Social Experiences Of Male Senior High School Students With Pretty Privilege, Vhinx Jazen G. Laguyo, Janoah Jamin L. Abibuag, Skylhar B. Custodio, Danica Joyce B. Tagure Jun 2024

Strikingly Attractive: Social Experiences Of Male Senior High School Students With Pretty Privilege, Vhinx Jazen G. Laguyo, Janoah Jamin L. Abibuag, Skylhar B. Custodio, Danica Joyce B. Tagure

DLSU Senior High School Research Congress

Physical attractiveness is often associated with various advantages, leading to the concept of "pretty privilege.” This study aimed to explore the social experiences of male Senior High School students who are considered physically attractive or have pretty privileges. This paper used a phenomenological design and obtained data through semi-structured interviews that were analyzed through thematic analysis. The findings revealed a range of positive and negative experiences that the male students encountered. Some of these social experiences are receiving compliments, being given more opportunities, being called a womanizer, and being expected to be smart and always look attractive.

Go to article

Investigating Chatgpt's Performance In Students' Academic Writing, Robian M. Esmas, Peter Ivan A. Florendo, Joellianne Grace B. Somontina, Diana Nicole F. Malantic, Jackylyn L. Beredo Jun 2024

Investigating Chatgpt's Performance In Students' Academic Writing, Robian M. Esmas, Peter Ivan A. Florendo, Joellianne Grace B. Somontina, Diana Nicole F. Malantic, Jackylyn L. Beredo

DLSU Senior High School Research Congress

As Artificial Intelligence (AI) continues to evolve, its integration into various domains has become increasingly notable. One prominent example is ChatGPT, a software tool that has gained popularity as an academic writing assistant. Despite its popularity, ChatGPT has sparked controversies due to concerns about potential misuse and exploitation. Studies have identified several writing challenges students face, especially those learning English as a foreign language (EFL), including grammar and spelling, vocabulary, paraphrasing, and referencing. This study evaluates ChatGPT 3.5's effectiveness in addressing these specific writing skills. Participants composed of Senior High School (SHS) students were assigned an academic writing task with …

Go to article

Initial Assessment And Floral Inventory Of V Ascular Plant Species In Angat Dam Watershed, Barangay Kabayunan, Doña Remedios Trinidad, Bulacan, Angela Mae Y. Coloma, Hannah Jelyn P. De Chavez, Ma. Hanielyn Q. Gadin, Sophia Ysabel A. Ronas Jun 2024

Initial Assessment And Floral Inventory Of V Ascular Plant Species In Angat Dam Watershed, Barangay Kabayunan, Doña Remedios Trinidad, Bulacan, Angela Mae Y. Coloma, Hannah Jelyn P. De Chavez, Ma. Hanielyn Q. Gadin, Sophia Ysabel A. Ronas

DLSU Senior High School Research Congress

The Philippines faces significant environmental challenges leading to rapid biodiversity loss. The Angat Watershed, vital for unique fauna and flora, maintains water quality for the Angat Hydroelectric Dam, supplying Metro Manila and nearby provinces. However, its flora is understudied, hindering plant protection. This study aims to assess and inventory vascular flora in Barangay Kabayunan, Angat Watershed, Doña Remedios Trinidad, Bulacan. Using purposive sampling and morphological assessment, 41 species (22 shrubs, 11 herbs, five trees, three epiphytes) from 38 genera and 24 families were collected, including 12 Philippine endemics, 20 natives, and one naturalized species. They were preserved in herbarium sheets …

Go to article

Exploration Of Carabao Mango Peel As A Source Of Natural Dye For Use In Cotton Fabrics, Nathaniel F. De Guzman, Colonel Sherlon Vance Iii V. Gonzales, Julienne Skye L. Lim, Christopher James C. Yap Jun 2024

Exploration Of Carabao Mango Peel As A Source Of Natural Dye For Use In Cotton Fabrics, Nathaniel F. De Guzman, Colonel Sherlon Vance Iii V. Gonzales, Julienne Skye L. Lim, Christopher James C. Yap

DLSU Senior High School Research Congress

The textile industry faces challenges in its dyeing processes due to the toxicity of synthetic dyes, significantly endangering the environment and human health. This study explores the use of natural dyes as a sustainable substitute for synthetic dyes in the textile industry, aiming to mitigate these environmental and health risks. The peels of Carabao mango (Mangifera indica), which are abundant in the Philippines, were utilized as a sustainable and eco-friendly alternative source of natural dyes. An aqueous extraction technique was employed to obtain natural dyes from mango peels. A synthetic dye, the Master fast dye, was used as a reference …

Go to article

Gold In Agricultural Waste: Sugarcane (S. Officinarum L.) Bagasse Cellulose As A Component For Starch-Based Bioplastic, Ellyza Marie C. Violante, Mayleen A. Alvarez Jun 2024

Gold In Agricultural Waste: Sugarcane (S. Officinarum L.) Bagasse Cellulose As A Component For Starch-Based Bioplastic, Ellyza Marie C. Violante, Mayleen A. Alvarez

DLSU Senior High School Research Congress

Hailing as the largest crop by volume of production in the Philippines, sugarcane (Saccharum officinarum L.) is consequently one of the nation’s topmost sources of agricultural residue. Unfortunately, the recovery efforts to employ sugarcane bagasse (SCB) as a biofuel were nullified due to the costliness of the proposition. Hence, sugar mill subsidiaries persisted with the improper aquatic disposal of SCB in Batangas. In response to this, the researchers harnessed the high cellulose content of this biomass in a bioplastic film, with the objective of establishing a research ground for SCB recycling, whilst promoting the relentless pursuit of sustainable communities. This …

Go to article

Decision In Art. 78 Proceeding - Green, Yvette (2024-05-03) Jun 2024

Decision In Art. 78 Proceeding - Green, Yvette (2024-05-03)

Decisions in Art. 78 Proceedings

No abstract provided.

Go to article

The Correlation Between Students’ Academic Workload And Salivary Ph Levels, Yves Zidane J. Biscocho, Lorenzo Miguel S. Carpio, Giovanni Paolo C. Salceda Jun 2024

The Correlation Between Students’ Academic Workload And Salivary Ph Levels, Yves Zidane J. Biscocho, Lorenzo Miguel S. Carpio, Giovanni Paolo C. Salceda

DLSU Senior High School Research Congress

Stress has been known to have negative and positive effects on humans. Studies have shown that academic stress, which comes from overwhelming academic demands, causes various negative effects on the well-being of students. Salivary cortisol levels can also be a predictor of academic stress. Concurrently, salivary pH levels are affected by changes in the salivary cortisol, and it can be inferred that salivary pH levels can predict the increase in academic workloads. To prove this relationship between students’ academic workloads and salivary pH levels, the researchers conducted a study utilizing a prospective research design. The data was collected from sixteen …

Go to article

"And If Thy Right Hand Offend Thee, Cut It Off": Human Sterilization, The Press & Universities, And "The Science Of Surgery" In Oregon, 1904-1940, Audrey Hart Jun 2024

"And If Thy Right Hand Offend Thee, Cut It Off": Human Sterilization, The Press & Universities, And "The Science Of Surgery" In Oregon, 1904-1940, Audrey Hart

Chronos

No abstract provided.

Go to article

Gardner-Webb Team Partners With Kudvumisa Foundation In Eswatini/Swaziland, Office Of University Communications Jun 2024

Gardner-Webb Team Partners With Kudvumisa Foundation In Eswatini/Swaziland, Office Of University Communications

Gardner-Webb NewsCenter Archive

Kudvumisa means “to praise” in the language of the people in Eswatini/Swaziland. It’s also the name chosen by Kudvumisa Foundation, an organization that provides medical clinics to people in this African county where medical care is lacking and HIV/Aids is prevalent.

Go to article

Transforming Orthopedic Surgery: Autonomous Image-Guided Techniques For Femur Fracture Robotic Surgery, Marzieh Sadat Saeedi-Hosseiny Jun 2024

Transforming Orthopedic Surgery: Autonomous Image-Guided Techniques For Femur Fracture Robotic Surgery, Marzieh Sadat Saeedi-Hosseiny

Theses and Dissertations

Long-bone fractures, particularly femur fractures, are prevalent and necessitate surgical intervention due to traumatic forces. Manual reduction procedures pose challenges, including excessive traction forces and reliance on limited X-ray imaging, resulting in malalignment-related complications and repeated surgeries. Current methods for femur fracture reduction are prone to inaccuracies, prolonged surgical times, and postoperative complications. This research introduces image-guided methods to automate a surgical robotic system, Robossis, to enhance femur alignment accuracy, reduce procedure times, and improve patient outcomes. To address these challenges, a marker-based approach utilizing a few X-ray images is developed to detect the spatial relative position of the femur …

Go to article

Under Pressure: The Interplay Of Hypertension And White Matter Hyperintensities With Cognition In Chronic Stroke Aphasia, Jade Hannan, Natalie Busby, Rebecca Roth, Janina Wilmskoetter, Roger Newman-Norlund Ph.D., Chris Rorden, Leonardo Bonilha, Julius Fridriksson Ph.D. Jun 2024

Under Pressure: The Interplay Of Hypertension And White Matter Hyperintensities With Cognition In Chronic Stroke Aphasia, Jade Hannan, Natalie Busby, Rebecca Roth, Janina Wilmskoetter, Roger Newman-Norlund Ph.D., Chris Rorden, Leonardo Bonilha, Julius Fridriksson Ph.D.

Faculty Publications

While converging research suggests that increased white matter hyperintensity load is associated with poorer cognition, and the presence of hypertension is associated with increased white matter hyperintensity load, the relationship among hypertension, cognition and white matter hyperintensities is not well understood. We sought to determine the effect of white matter hyperintensity burden on the relationship between hypertension and cognition in individuals with post-stroke aphasia, with the hypothesis that white matter hyperintensity load moderates the relationship between history of hypertension and cognitive function. Health history, Fazekas scores for white matter hyperintensities and Wechsler Adult Intelligence Scale Matrix Reasoning subtest scores for …

Go to article

Design And Fabrication Of Passive Microfluidic Devices For Enhancing Membrane-Based Biosensors, Tanner N. Wells Jun 2024

Design And Fabrication Of Passive Microfluidic Devices For Enhancing Membrane-Based Biosensors, Tanner N. Wells

Theses and Dissertations

Microfluidic devices and systems have demonstrated wide applicability in sample processing and analysis. The ability to integrate sample processing and analysis into a single platform is a key benefit of microfluidic technologies. This dissertation describes the design and fabrication two types of microfluidic devices developed to enhance membrane-based biosensors. First is a device for collecting particles into a highly confined region, locally enhancing their concentration. Second is a device for size-based particle separation capable of separating differently sized particles into separate streams. Both types of devices are fabricated with a thin top layer, providing a platform for synthetic membrane-based technologies. …

Go to article

The Design, Prototyping, And Validation Of A New Wearable Sensor System For Monitoring Lumbar Spinal Motion In Daily Activities, Brianna Bischoff Jun 2024

The Design, Prototyping, And Validation Of A New Wearable Sensor System For Monitoring Lumbar Spinal Motion In Daily Activities, Brianna Bischoff

Theses and Dissertations

Lower back pain is a widespread problem affecting millions worldwide, because understanding its development and effective treatment remains challenging. Current treatment success is often evaluated using patient-reported outcomes, which tend to be qualitative and subjective in nature, making objective success measurement difficult. Wearable sensors can provide quantitative measurements, thereby helping physicians improve care for countless individuals around the world. These sensors also have the potential to provide longitudinal data on daily motion patterns, aiding in monitoring the progress of treatment plans for lower back pain. In this work it was hypothesized that a new wearable sensor garment that makes use …

Go to article

Human Service Professionals’ Experiences Using Mindfulness-Based Interventions To Support Depression And Suicidal Ideation Treatment, Luke Oluwadamilare Joseph Jun 2024

Human Service Professionals’ Experiences Using Mindfulness-Based Interventions To Support Depression And Suicidal Ideation Treatment, Luke Oluwadamilare Joseph

Walden Dissertations and Doctoral Studies

Depression and suicide ideation are mental health disorders that have seen a sharp increase recently and impact the global population, with adverse effects on the public health of the entire United States. Researchers have shown that mindfulness-based interventions (MBIs) are an evolving phenomenon used in addressing common mental challenges, such as depression and suicide, resulting in improved psychosocial and psychological stability. The purpose of this generic study was to explore the experiences of human service professionals (HSPs) who use MBI to support clients with depression and suicidal ideation in Chicago. Seven purposively sampled HSPs were the participants in the study. …

Go to article

Petit ’26 Earns Noaa Hollings Scholarship, Keith Testa Jun 2024

Petit ’26 Earns Noaa Hollings Scholarship, Keith Testa

UNH Today Archive

No abstract provided.

Go to article

The Aquatic Microbial Environment Shapes The Gut Microbiota, Brain, And Behavior Of Larval Amphibians, Kyle Emerson Jun 2024

The Aquatic Microbial Environment Shapes The Gut Microbiota, Brain, And Behavior Of Larval Amphibians, Kyle Emerson

Electronic Theses and Dissertations

Microbial communities comprising bacteria, viruses, fungi, and protists live within and on the surfaces of animal hosts. These microbial communities exist in symbiosis with the host, and heavily influence host physiology, development, health, and fitness. Gut-dwelling microbes (i.e., gut microbiota) contribute to host neurodevelopment through a bidirectional Microbiota-Gut-Brain (MGB) axis. Evidence of the MGB axis has been primarily derived from studies that use germ-free (GF) models, which commonly display altered neurophysiology and behavior compared to conventionally raised counterparts. Almost all studies of the MGB axis have used mammalian models in a biomedical framework, leaving a knowledge gap regarding the role …

Go to article

Boom, Shake The Room: Seismic Surveys Affect Behaviour And Survival Of Western Rock Lobster, Simon De Lestang, Jason How, Kelvin Rushworth, Christine Erbe Jun 2024

Boom, Shake The Room: Seismic Surveys Affect Behaviour And Survival Of Western Rock Lobster, Simon De Lestang, Jason How, Kelvin Rushworth, Christine Erbe

Fisheries Research Articles

Seismic studies are conducted to investigate subterranean structures for, among other things, indications of petroleum and mineral deposits. Within the marine environment, these studies use sub-surface airguns to create powerful sound waves that penetrate the substrate, with their variable reflection time and strength being used to model seabed densities, structure, and composition. There is a growing body of work examining the effects of seismic surveys on marine fauna, primarily marine mammals and to a lesser extent fishes and invertebrates. Recently published studies on the southern rock lobster have shown these intense sound waves can affect lobster physiology and short-term behaviour. …

Go to article

The Mrnacalc Webserver Accounts For The N1-Methylpseudouridine Hypochromicity To Enable Precise Nucleoside-Modified Mrna Quantification, Sarah E. Krul, Sean J. Hoehn, Carlos E. Crespo-Hernández Jun 2024

The Mrnacalc Webserver Accounts For The N1-Methylpseudouridine Hypochromicity To Enable Precise Nucleoside-Modified Mrna Quantification, Sarah E. Krul, Sean J. Hoehn, Carlos E. Crespo-Hernández

Faculty Scholarship

Nucleoside-modified messenger RNA (mRNA) technologies necessarily incorporate N1-methylpseudouridine into the mRNA molecules to prevent the over-stimulation of cytoplasmic RNA sensors. Despite this modification, mRNA concentrations remain mostly determined through the measurement of UV absorbance at 260 nm wavelength (A260). Herein, we report that the N1-methylpseudouridine absorbs approximately 40% less UV light at 260 nm than uridine, and its incorporation into mRNAs leads to the under-estimation of nucleoside-modified mRNA concentrations, with 5%–15% error, in an mRNA-sequence-dependent manner. We therefore examined the RNA quantification methods and developed the mRNACalc webserver. It accounts for the molar absorption coefficient of modified nucleotides at 260 …

Go to article

An Inducible Genetic Tool To Track And Manipulate Specific Microglial States Reveals Their Plasticity And Roles In Remyelination, Kia M Barclay, Nora Abduljawad, Zuolin Cheng, Min Woo Kim, Lu Zhou, Jin Yang, Justin Rustenhoven, Jose A Mazzitelli, Leon C D Smyth, Dvita Kapadia, Simone Brioschi, Wandy Beatty, Jinchao Hou, Naresha Saligrama, Marco Colonna, Guoqiang Yu, Jonathan Kipnis, Qingyun Li Jun 2024

An Inducible Genetic Tool To Track And Manipulate Specific Microglial States Reveals Their Plasticity And Roles In Remyelination, Kia M Barclay, Nora Abduljawad, Zuolin Cheng, Min Woo Kim, Lu Zhou, Jin Yang, Justin Rustenhoven, Jose A Mazzitelli, Leon C D Smyth, Dvita Kapadia, Simone Brioschi, Wandy Beatty, Jinchao Hou, Naresha Saligrama, Marco Colonna, Guoqiang Yu, Jonathan Kipnis, Qingyun Li

The Brown Foundation: Institute of Molecular Medicine

Recent single-cell RNA sequencing studies have revealed distinct microglial states in development and disease. These include proliferative region-associated microglia (PAM) in developing white matter and disease-associated microglia (DAM) prevalent in various neurodegenerative conditions. PAM and DAM share a similar core gene signature. However, the extent of the dynamism and plasticity of these microglial states, as well as their functional significance, remains elusive, partly due to the lack of specific tools. Here, we generated an inducible Cre driver line, Clec7a-CreERT2, that targets PAM and DAM in the brain parenchyma. Utilizing this tool, we profiled labeled cells during development and in several …

Go to article

Slow Proliferation Of Bap1-Deficient Uveal Melanoma Cells Is Associated With Reduced S6 Signaling And Resistance To Nutrient Stress, Vivian Chua, Melisa Lopez-Anton, Mizue Terai, Ryota Tanaka, Usman Baqai, Timothy J Purwin, Jelan I Haj, Francis J Waltrich, Isabella Trachtenberg, Kristine Luo, Rohith Tudi, Angela Jeon, Anna Han, Inna Chervoneva, Michael A Davies, Julio A Aguirre-Ghiso, Takami Sato, Andrew E Aplin Jun 2024

Slow Proliferation Of Bap1-Deficient Uveal Melanoma Cells Is Associated With Reduced S6 Signaling And Resistance To Nutrient Stress, Vivian Chua, Melisa Lopez-Anton, Mizue Terai, Ryota Tanaka, Usman Baqai, Timothy J Purwin, Jelan I Haj, Francis J Waltrich, Isabella Trachtenberg, Kristine Luo, Rohith Tudi, Angela Jeon, Anna Han, Inna Chervoneva, Michael A Davies, Julio A Aguirre-Ghiso, Takami Sato, Andrew E Aplin

Faculty, Staff and Student Publications

Uveal melanoma (UM) is the deadliest form of eye cancer in adults. Inactivating mutations and/or loss of expression of the gene encoding BRCA1-associated protein 1 (BAP1) in UM tumors are associated with an increased risk of metastasis. To investigate the mechanisms underlying this risk, we explored the functional consequences of BAP1 deficiency. UM cell lines expressing mutant BAP1 grew more slowly than those expressing wild-type BAP1 in culture and in vivo. The ability of BAP1 reconstitution to restore cell proliferation in BAP1-deficient cells required its deubiquitylase activity. Proteomic analysis showed that BAP1-deficient cells had decreased phosphorylation of ribosomal S6 and …

Go to article

Role Of Ethanolamine Utilization And Bacterial Microcompartment Formation In Listeria Monocytogenes Intracellular Infection, Ayan Chatterjee, Karan Gautam Kaval, Danielle A Garsin Jun 2024

Role Of Ethanolamine Utilization And Bacterial Microcompartment Formation In Listeria Monocytogenes Intracellular Infection, Ayan Chatterjee, Karan Gautam Kaval, Danielle A Garsin

Faculty, Staff and Student Publications

Ethanolamine (EA) affects the colonization and pathogenicity of certain human bacterial pathogens in the gastrointestinal tract. However, EA can also affect the intracellular survival and replication of host cell invasive bacteria such as Listeria monocytogenes (LMO) and Salmonella enterica serovar Typhimurium (S. Typhimurium). The EA utilization (eut) genes can be categorized as regulatory, enzymatic, or structural, and previous work in LMO showed that loss of genes encoding functions for the enzymatic breakdown of EA inhibited LMO intracellular replication. In this work, we sought to further characterize the role of EA utilization during LMO infection of host …

Go to article

Predicting Ph At In-Situ Temperature For Aquatic Environments, Sarah E. Bartoloni Jun 2024

Predicting Ph At In-Situ Temperature For Aquatic Environments, Sarah E. Bartoloni

USF Tampa Graduate Theses and Dissertations

pH is typically measured at a constant temperature and, as needed, converted to in-situ temperature using a system of thermodynamic equations. Using this equilibrium approach, adjustment of pH to in situ conditions requires two measured CO2 system parameters (pH, total alkalinity, dissolved inorganic carbon, or CO2 fugacity). Currently, when pH is the only measured CO2 system parameter, linear models are used to adjust the pH to in-situ temperature. Although many studies have attempted to optimize CO2 equilibrium constants (K1 and K2) for the CO2 system over a range of conditions, very little prior work has experimentally examined which CO2 system …

Go to article

Manipulating Colloidal Particles Using Chemical Gradients And Top-Down Control, Mark Nichols Mcdonald Jun 2024

Manipulating Colloidal Particles Using Chemical Gradients And Top-Down Control, Mark Nichols Mcdonald

Theses and Dissertations

Colloidal particles provide the ideal building blocks for the next generation of microdevices, such as advanced sensors and precision drug delivery systems. However, many such applications require the use of top-down (i.e. humanly controllable) forces to manipulate colloidal particles with single-particle precision, and current methods can only achieve such precision for small numbers of particles at a time. To address this challenge, we propose using chemical forces in combination with existing top-down techniques to enable the control of larger numbers of particles simultaneously. Controlling colloids using chemical reactions is a novel technique not typically utilized. Due to its distinct difference …

Go to article

Painful Shoulder Syndrome And Upper Limb Function In Competitive Swimmers, Beata Pożarowszczyk-Kuczko, Martyna Kumorek, Dariusz Mroczek, Filipe Clemente, Wioletta Dziubek Jun 2024

Painful Shoulder Syndrome And Upper Limb Function In Competitive Swimmers, Beata Pożarowszczyk-Kuczko, Martyna Kumorek, Dariusz Mroczek, Filipe Clemente, Wioletta Dziubek

Baltic Journal of Health and Physical Activity

Introduction. Swimming is an athletic discipline that largely involves the upper limbs. Due to the driving and propulsive function, there is a heavy involvement of almost all muscles of the arm. Painful shoulder syndrome (PSS) is considered to be one of the main causes of interference with an effective and correct movement of the underwater stroke, which is a fundamental element of functionality in this discipline. The aim of this study was to evaluate whether PSS affects upper limb function in competitive swimmers. Material and methods. Thirty-two swimmers aged 17–24 years with at least 2nd sport class participated in the …

Go to article

Outcomes Of Subsequent Antilymphoma Therapies After Second-Line Axicabtagene Ciloleucel Or Standard Of Care In Zuma-7, Armin Ghobadi, Et Al. Jun 2024

Outcomes Of Subsequent Antilymphoma Therapies After Second-Line Axicabtagene Ciloleucel Or Standard Of Care In Zuma-7, Armin Ghobadi, Et Al.

2020-Current year OA Pubs

The optimal management of patients with relapsed/refractory large B-cell lymphoma (LBCL) after disease progression or lack of response to second-line (2L) therapy remains unclear. Here, we report outcomes among patients who received subsequent antilymphoma therapy per investigator discretion separately by their randomized 2L arm in ZUMA-7, namely axicabtagene ciloleucel (axi-cel) vs standard of care (SOC). Progression-free survival (PFS) and overall survival (OS) were calculated from 3L therapy initiation. In the SOC arm, 127 of 179 randomized patients (71%) received 3L therapy. Median PFS among those who received 3L cellular immunotherapy (n = 68) vs those who did not (n = …

Go to article

Limited Nerve Regeneration Across Acellular Nerve Allografts (Anas) Coincides With Changes In Blood Vessel Morphology And The Development Of A Pro-Inflammatory Microenvironment, Jesús A Acevedo Cintrón, Daniel A Hunter, Lauren Schellhardt, Deng Pan, Susan E Mackinnon, Matthew D Wood Jun 2024

Limited Nerve Regeneration Across Acellular Nerve Allografts (Anas) Coincides With Changes In Blood Vessel Morphology And The Development Of A Pro-Inflammatory Microenvironment, Jesús A Acevedo Cintrón, Daniel A Hunter, Lauren Schellhardt, Deng Pan, Susan E Mackinnon, Matthew D Wood

2020-Current year OA Pubs

The use of acellular nerve allografts (ANAs) to reconstruct long nerve gaps (>3 cm) is associated with limited axon regeneration. To understand why ANA length might limit regeneration, we focused on identifying differences in the regenerative and vascular microenvironment that develop within ANAs based on their length. A rat sciatic nerve gap model was repaired with either short (2 cm) or long (4 cm) ANAs, and histomorphometry was used to measure myelinated axon regeneration and blood vessel morphology at various timepoints (2-, 4- and 8-weeks). Both groups demonstrated robust axonal regeneration within the proximal graft region, which continued across …

Go to article

Frontotemporal Dementia-Like Disease Progression Elicited By Seeded Aggregation And Spread Of Fus, Sonia Vazquez-Sanchez, Britt Tilkin, Fatima Gasset-Rosa, Sitao Zhang, Diana Piol, Melissa Mcalonis-Downes, Jonathan Artates, Noe Govea-Perez, Yana Verresen, Lin Guo, Don Cleveland, James Shorter, Sandrine Da Cruz Jun 2024

Frontotemporal Dementia-Like Disease Progression Elicited By Seeded Aggregation And Spread Of Fus, Sonia Vazquez-Sanchez, Britt Tilkin, Fatima Gasset-Rosa, Sitao Zhang, Diana Piol, Melissa Mcalonis-Downes, Jonathan Artates, Noe Govea-Perez, Yana Verresen, Lin Guo, Don Cleveland, James Shorter, Sandrine Da Cruz

Department of Biochemistry and Molecular Biology Faculty Papers

RNA binding proteins have emerged as central players in the mechanisms of many neurodegenerative diseases. In particular, a proteinopathy of fused in sarcoma (FUS) is present in some instances of familial Amyotrophic lateral sclerosis (ALS) and about 10% of sporadic Frontotemporal lobar degeneration (FTLD). Here we establish that focal injection of sonicated human FUS fibrils into brains of mice in which ALS-linked mutant or wild-type human FUS replaces endogenous mouse FUS is sufficient to induce focal cytoplasmic mislocalization and aggregation of mutant and wild-type FUS which with time spreads to distal regions of the brain. Human FUS fibril-induced FUS aggregation …

Go to article

Tenovin-1, A Selective Sirt1/2 Inhibitor, Attenuates High-Fat Diet-Induced Hepatic Fibrosis Via Inhibition Of Hsc Activation In Zdf Rats, Swati Sharma, Sreevarsha Gali, Amit Kundu, Jae Hyeon Park, Jae-Sung Kim, Hyung Sik Kim Jun 2024

Tenovin-1, A Selective Sirt1/2 Inhibitor, Attenuates High-Fat Diet-Induced Hepatic Fibrosis Via Inhibition Of Hsc Activation In Zdf Rats, Swati Sharma, Sreevarsha Gali, Amit Kundu, Jae Hyeon Park, Jae-Sung Kim, Hyung Sik Kim

2020-Current year OA Pubs

Type 2 diabetes mellitus (T2DM) increases the risk of non-alcoholic fatty liver disease (NAFLD) progression to advanced stages, especially upon high-fat diet (HFD). HFD-induced hepatic fibrosis can be marked by oxidative stress, inflammation, and activation of hepatic stellate cells. Sirtuin 1/2 (SIRT1/2), NAD-dependent class III histone deacetylases, are involved in attenuation of fibrosis. In our conducted research, TGF-β1-activated LX-2 cells, free fatty acid (FFA)-treated simultaneous co-culture (SCC) cells, and HFD-induced hepatic fibrosis in Zucker diabetic fatty (ZDF) rats, a widely used animal model in the study of metabolic syndromes, were used to evaluate the protective effect of Tenovin-1, a SIRT1/2 …

Go to article

Rigor And Reproducibility In Human Brain Organoid Research: Where We Are And Where We Need To Go, Soraya O Sandoval, Gerarda Cappuccio, Karina Kruth, Sivan Osenberg, Saleh M Khalil, Natasha M Méndez-Albelo, Krishnan Padmanabhan, Daifeng Wang, Mark J Niciu, Anita Bhattacharyya, Jason L Stein, André M M Sousa, Elisa A Waxman, Elizabeth D Buttermore, Dosh Whye, Carissa L Sirois, Cross-Iddrc Human Stem Cell Consortium, Aislinn Williams, Mirjana Maletic-Savatic, Xinyu Zhao Jun 2024

Rigor And Reproducibility In Human Brain Organoid Research: Where We Are And Where We Need To Go, Soraya O Sandoval, Gerarda Cappuccio, Karina Kruth, Sivan Osenberg, Saleh M Khalil, Natasha M Méndez-Albelo, Krishnan Padmanabhan, Daifeng Wang, Mark J Niciu, Anita Bhattacharyya, Jason L Stein, André M M Sousa, Elisa A Waxman, Elizabeth D Buttermore, Dosh Whye, Carissa L Sirois, Cross-Iddrc Human Stem Cell Consortium, Aislinn Williams, Mirjana Maletic-Savatic, Xinyu Zhao

Faculty, Staff and Students Publications

Human brain organoid models have emerged as a promising tool for studying human brain development and function. These models preserve human genetics and recapitulate some aspects of human brain development, while facilitating manipulation in an in vitro setting. Despite their potential to transform biology and medicine, concerns persist about their fidelity. To fully harness their potential, it is imperative to establish reliable analytic methods, ensuring rigor and reproducibility. Here, we review current analytical platforms used to characterize human forebrain cortical organoids, highlight challenges, and propose recommendations for future studies to achieve greater precision and uniformity across laboratories.

Go to article

First Prev Next Last