Digital Commons Network^™

Anonymized Identity Recognition And Classification Using Privacy Preserving Facial Encoding, Manas Sanjay Pakalapati

USF Tampa Graduate Theses and Dissertations

The need for sharing large-scale datasets to train deep neural network models, particularly in healthcare, raises significant data security and privacy concerns. To address these issues, methods such as data encryption or encoding are utilized. These techniques can encrypt the data and make it unreadable to humans, while still retaining its usefulness for training models.

In this study, we investigate various image encoding techniques designed to protect privacy by making images unrecognizable while still retaining their usefulness for model training. Our investigation utilized publicly available facial databases and focused on evaluating the trade-offs inherent in image encoding techniques, with a …

Evaluation Of A Clincally-Integrated Community Health Worker Program To Support Adults With Cardiovascular Conditions: Guided By The Prism Framework, Cynthia Orofo

Dissertations

Chronically ill individuals with cardiovascular diseases (CVDs) have been extensively challenged by the complexities of disease management. Cardiovascular disease remains the leading cause of death in the U.S. Although clinically integrated community health worker (cCHW) support interventions have been increasingly implemented to help manage CVDs, a comprehensive analysis of interventions implemented in real-world settings is lacking. The purpose of this study was to observe and evaluate a cCHW support intervention in a real clinical setting serving adults with CVD using an implementation science perspective, guided by the PRISM Framework.

Discovery Of Runs-Of-Homozygosity Diplotype Clusters And Their Associations With Diseases In Uk Biobank, Ardalan Naseri, Degui Zhi, Shaojie Zhang

Faculty, Staff and Student Publications

Runs-of-homozygosity (ROH) segments, contiguous homozygous regions in a genome were traditionally linked to families and inbred populations. However, a growing literature suggests that ROHs are ubiquitous in outbred populations. Still, most existing genetic studies of ROH in populations are limited to aggregated ROH content across the genome, which does not offer the resolution for mapping causal loci. This limitation is mainly due to a lack of methods for the efficient identification of shared ROH diplotypes. Here, we present a new method, ROH-DICE (runs-of-homozygous diplotype cluster enumerator), to find large ROH diplotype clusters, sufficiently long ROHs shared by a sufficient number …

Novel Adaptations In Iron Regulation Acquired During Chronic Fungal Cf Infections, Daniel R. Murante

Dartmouth College Ph.D Dissertations

Chronic fungal infections are highly recalcitrant to treatment; we postulated that as populations persist, increasing genetic diversity is reflected in phenotypic heterogeneity, contributing to treatment inefficacies. The study of evolutionary patterns is underrepresented in chronic fungal infections, and to supplement this body of knowledge, we leveraged isolates acquired from four individuals with chronic fungal-dominated cystic fibrosis infections. We evaluated in-host evolution through a whole-genome sequencing approach, comparing multiple isolates obtained from each subject's sputum and bronchoalveolar lavage fluid. Our analysis found non-synonymous mutations that arose in parallel across the independent infections in the gene MRS4, which encodes a mitochondrial …

Meta-Theoretic Competence For Computational Agent-Based Modeling, Hillary Swanson, Uri Wilensky

Instructional Technology and Learning Sciences Faculty Publications

In the U.S. context, science standards encourage educators to engage students in modeling practices, including computational modeling. While much work has investigated the productivity of computational modeling with respect to students' development of scientific content knowledge, less work has focused on students' development of knowledge and skills for participation in computational modeling practices. A first step in understanding how these practices develop is examining students' activity in the context of computational modeling environments with attention to the productive moves they make. These moves can provide insight into the knowledge they bring to their learning, which may be foundational to the …

What Happened To The Geometry? Examining Spatial And Mathematical Concepts In Computational Toys And Kits For Young Children, Jody Clarke-Midura, Jessica F. Shumway, Anahita Ashineh, Alireza Zandi, Whitney Clawson

Instructional Technology and Learning Sciences Faculty Publications

This paper explores the design of computational toys and kits for young children (ages 4-7) as tools for learning integrated mathematical, spatial, and computational thinking concepts. Specifically, we examine how the design features of the toys and kits represent the concepts of rotation on a point and spatial orientation of the agent. We examine toys and kits sold commercially, developed through research, and used in early childhood classrooms. Our findings indicate that the mathematical and spatial concepts are overlooked in some designs. Prior research examined toys for their affordances related to computational thinking, the present study contributes to understanding of …

Aso Author Reflections: Isolated Margin Positivity In Localized Pancreatic Ductal Adenocarcinoma And Operative Margin Revision, Lee M. Ocuin

Faculty Scholarship

No abstract provided.

Evaluating The Effects Of Cheatgrass On Western Burrowing Owls, Kaylee R. Draughon

Theses and Dissertations

There has been a global decline of specialist species observed in recent decades due to the impacts of climate change, invasive species, and habitat loss. Habitat loss and degradation may lead to a mismatch between habitat attractiveness and actual quality, otherwise known as an ecological trap. Ecological traps occur when an organism is constrained by its evolutionary past to select for cues that no longer accurately predict habitat quality. Specialist species are more susceptible to ecological traps due to greater reliance on and fidelity to historic sites and resources. The burrowing owl (Athene cunicularia), a specialist bird species adapted to …

Multiple Omics Levels Of Chronic Lymphocytic Leukemia, Aleksander Turk, Eva Čeh, George A Calin, Tanja Kunej

Faculty, Staff and Student Publications

Chronic lymphocytic leukemia (CLL) is a lymphoproliferative malignancy characterized by the proliferation of functionally mature but incompetent B cells. It is the most prevalent type of leukemia in Western populations, accounting for approximately 25% of new leukemia cases. While recent advances, such as ibrutinib and venetoclax treatment have improved patient outlook, aggressive forms of CLL such as Richter transformation still pose a significant challenge. This discrepancy may be due to the heterogeneity of factors contributing to CLL development at multiple -omics levels. However, information on the omics of CLL is fragmented, hindering multi-omics-based research into potential treatment options. To address …

Study Of Prognostic Splicing Factors In Cancer Using Machine Learning Approaches, Mengyuan Yang, Jiajia Liu, Pora Kim, Xiaobo Zhou

Faculty, Staff and Student Publications

Splicing factors (SFs) are the major RNA-binding proteins (RBPs) and key molecules that regulate the splicing of mRNA molecules through binding to mRNAs. The expression of splicing factors is frequently deregulated in different cancer types, causing the generation of oncogenic proteins involved in cancer hallmarks. In this study, we investigated the genes that encode RNA-binding proteins and identified potential splicing factors that contribute to the aberrant splicing applying a random forest classification model. The result suggested 56 splicing factors were related to the prognosis of 13 cancers, two SF complexes in liver hepatocellular carcinoma, and one SF complex in esophageal …

Protocol For Seahorse Analysis Of Ex Vivo Mouse Brown And White Adipose Tissues, Fenfen Wang, Phu M Huynh, Yu A An

Faculty, Staff and Student Publications

The mitochondrial stress test is a gold-standard approach for assessing adipose tissue physiological functions and pathological changes. Here, we present a protocol for conducting Seahorse assays using ex vivo mouse brown and white adipose depots. We describe steps for rehydrating the cartridge, preparing freshly harvested fat depots, placing them onto an islet capture plate, and incubating them in a non-CO2 incubator. We then detail procedures for adding mitochondrial stressor solutions and conducting the mitochondrial stress test using the Seahorse XFe24 Analyzer. For complete details on the use and execution of this protocol, please refer to An et al.1.

Comparative Genomics Of Anax (Odonata: Aeshnidae), Ying Fei Lin

Theses and Dissertations

Insect migration is an important phenomenon characterized by the long-distance travel of various species in search of resources, safety, breeding, and/or suitable climates. However, relatively little is known about insect migration patterns and the genetic factors associated with them. Three closely related species of dragonflies (Odonata: Aeshnidae), Anax junius, A. strenuus, and A. walsinghami, form a clade of migratory and non-migratory species and present a natural laboratory to study the evolution of genes that may be related to muscle developments and insect migration. In this study, we sequence the whole genomes of these three Anax species to present a resource …

Exploring Seizure Signal Processing And Methods To Characterize Seizure-Like Activity In Mouse Brains, Benjamin Kearsley

Undergraduate Honors Theses

Status Epilepticus (SE) is a dangerous type of seizure that is difficult to treat and can have permanent or fatal consequences. Developing methods to properly process and classify SE in Local Field Potential (LFP) signals are important steps towards being able to predict SE in clinic and save lives. This thesis explores methods for the seizure data processing LFP data with the goal of gaining a deeper understanding of the effect of brain region and tissue preparation paradigm on power output in specific frequency ranges. The brain regions compared are the neocortex and hippocampus, and the preparation paradigms are 4AP …

Enzymological Revisionism: Employing Transient State Approaches To Define The Catalytic Sequences Of Poorly Understood Systems, Madison M. Smith

Dissertations

Flavin is a molecule composed of a tricyclic isoalloxazine ring linked to a ribityl. Flavins are utilized as a cofactor by many enzymes to facilitate a myriad of chemistries. Flavoproteins were first discovered in the late 1800 s and have since been an interest to researchers, largely due to the unique spectrophotometric characteristics that make it a valuable reporter molecule. Flavin cofactors (typically either flavin adenine dinucleotide (FAD) or flavin mononucleotide (FMN)) are chemically versatile as the isoalloxazine ring can form one and two-electron reduced states. The reduced states are prone to react with molecular oxygen and numerous observations have …

Synthesis Of Streptococcus Pneumoniae Serotypes 6a And 6b Di- And Trisaccharides As Propargyl Glycosyl Antigens For Glycoconjugate Vaccines, Tianyao Meng

Theses and Dissertations

Streptococcus pneumoniae (Spn) is a globally prevalent Gram-positive pathogen responsible for invasive pneumococcal diseases (IPD). Among its over 90 serotypes, Spn serotypes 6A (Sp6A) and 6B (Sp6B) are two of the most common causes of IPD around the world and are more frequently isolated from infections than most of the other serotypes. Our group has previously developed a method for making immune responses to bacterial glycans T cell-dependent, involving attachment of short synthetic glycans to a virus-like nanoparticle (VLP), and in combination with a natural killer T cell (NKT cell) adjuvant. This strategy enhanced immune responses by facilitating T cell …

When John Locke Meets Lao Tzu: The Relationship Between Intellectual Property, Biodiversity, And Indigenous Knowledge And The Implications For Food Security, Paolo Davide Farah, Marek Prityi

Duke Environmental Law & Policy Forum

No abstract provided.

Adolescent Obesogenic Behaviors: The Influence Of Race/Ethnicity, Generation Status, Acculturation, And Tme Lived Outside The United States, Tien D. Vo, Alisha H. Redelfs, Mackay Graper, Lori A. Spruance

Journal of Social, Behavioral, and Health Sciences

Children living with obesity are at higher risk for chronic disease. Dietary behaviors, sedentary time, and physical activity contribute to the risk of developing obesity. The purpose of this research was to identify associations between acculturation and race/ethnicity influences on obesogenic behaviors in middle school students. A national convenience sample of diverse U.S. children (11–14) was recruited to participate in an online survey (N = 615). Dietary behaviors, including fruit and vegetable consumption (F/V) and sugar-sweetened beverage consumption (SSB), as well as screen time and physical activity, were quantified. Multivariable regression models (linear and logistic) were built. We analyzed …

The Academic Influence Of The Camino De Santiago: A Bibliometric Analysis Of Doctoral Research And Indexed Manuscripts, Silvia Díaz-De La Fuente, Virginia Ahedo, María Pilar Alonso Abad, José Manuel Galán

International Journal of Religious Tourism and Pilgrimage

The Camino de Santiago is one of the most recognised cultural pilgrimage routes in the world. Notably, its current influence transcends the strictly inspirational and religious aspects, constituting also a socio-economic, cultural, historical, heritage and tourism cornerstone. This paper analyses, from an innovative bibliometric perspective, the influence of the Camino de Santiago on the academic literature in the period from 1980 to the present. For this purpose, two data sources have been used comparatively: manuscripts indexed in Scopus and doctoral theses. The results reveal a growth of its academic relevance in the last decade, especially in indexed publications. The analysis …

The Association Between Mental Health And Fibromyalgia In African Americans, Shalika J. Tisinger

Walden Dissertations and Doctoral Studies

Depression and fibromyalgia can often exacerbate each other and are known to be bidirectional disorders. Chronic pain from fibromyalgia often leads to depression, and depression can cause pain to become worse and affect the nervous system, causing pain. The purpose of this quantitative, binary logistic regression study is to determine if there is an association between mental health and fibromyalgia in African Americans while controlling for cardiovascular disease, chronic kidney disease, arthritis, education level (ie. Some high school, high school graduate, some college or higher), diet, poverty-income-ratio, PIR, diabetes, gender, and age. The socio-ecological model included in this cross-sectional study …

Empathy And Political Skill: Improving Salespeople’S Value Enhancing Behavior Performance, Megan C. Good, Charles H. Schwepker Jr.

Journal of Applied Marketing Theory

Little is known about the interface between empathy and political skill in sales. This study develops a model to reflect these characteristics and their impact on performance. Results from a study of 240 B2B salespeople contradict the a priori assumption that empathy may not be positively related to sales performance and challenge existing stereotypes that salespeople are not empathetic. Importantly, the findings show sellers employing empathy can generate enhanced outcomes. Also, this study extends our understanding that utilizing political influence spreads the benefit of empathy beyond the impact itself. Politically skilled B2B salespeople can better understand and display their empathy …

The Association Between Mental Health And Fibromyalgia In African Americans, Shalika J. Tisinger

Walden Dissertations and Doctoral Studies

Depression and fibromyalgia can often exacerbate each other and are known to be bidirectional disorders. Chronic pain from fibromyalgia often leads to depression, and depression can cause pain to become worse and affect the nervous system, causing pain. The purpose of this quantitative, binary logistic regression study is to determine if there is an association between mental health and fibromyalgia in African Americans while controlling for cardiovascular disease, chronic kidney disease, arthritis, education level (ie. Some high school, high school graduate, some college or higher), diet, poverty-income-ratio, PIR, diabetes, gender, and age. The socio-ecological model included in this cross-sectional study …

Elucidating Immune-Related Gene Transcriptional Programs Via Factorization Of Large-Scale Rna-Profiles, Shan He, Matthew M Gubin, Hind Rafei, Rafet Basar, Merve Dede, Xianli Jiang, Qingnan Liang, Yukun Tan, Kunhee Kim, Maura L Gillison, Katayoun Rezvani, Weiyi Peng, Cara Haymaker, Sharia Hernandez, Luisa M Solis, Vakul Mohanty, Ken Chen

Faculty, Staff and Student Publications

Recent developments in immunotherapy, including immune checkpoint blockade (ICB) and adoptive cell therapy (ACT), have encountered challenges such as immune-related adverse events and resistance, especially in solid tumors. To advance the field, a deeper understanding of the molecular mechanisms behind treatment responses and resistance is essential. However, the lack of functionally characterized immune-related gene sets has limited data-driven immunological research. To address this gap, we adopted non-negative matrix factorization on 83 human bulk RNA sequencing (RNA-seq) datasets and constructed 28 immune-specific gene sets. After rigorous immunologist-led manual annotations and orthogonal validations across immunological contexts and functional omics data, we demonstrated …

A Case Study Of Layered Border Security And Traffic Problems At Laredo, Texas Port Of Entry, Christopher S. Castacio

Walden Dissertations and Doctoral Studies

Securing the border is the United States Government's top priority to keep terrorists, illegal weapons, nuclear weapons, human smuggling, and drug smuggling from entering the United States during the continuous crossing of legitimate international trades and legitimate travelers. The primary purpose of this qualitative research study was to examine layered U.S. border security and analyze the vehicle and pedestrian flow of traffic problems in Laredo, Texas, Port of Entry. Qualitative methodology was used in this study using seven interviews and open-ended questionnaires. This study's theories were the environment criminology theory, which explored and analyzed the criminal act's environment; and the …

A Case Study Of Layered Border Security And Traffic Problems At Laredo, Texas Port Of Entry, Christopher S. Castacio

Walden Dissertations and Doctoral Studies

Securing the border is the United States Government's top priority to keep terrorists, illegal weapons, nuclear weapons, human smuggling, and drug smuggling from entering the United States during the continuous crossing of legitimate international trades and legitimate travelers. The primary purpose of this qualitative research study was to examine layered U.S. border security and analyze the vehicle and pedestrian flow of traffic problems in Laredo, Texas, Port of Entry. Qualitative methodology was used in this study using seven interviews and open-ended questionnaires. This study's theories were the environment criminology theory, which explored and analyzed the criminal act's environment; and the …

Comparing The Genomes Of Multidrug And Vancomycin-Resistant Enterococcus Faecium From A Florida Wastewater Treatment Plant, Eleanor Brodrick

USF Tampa Graduate Theses and Dissertations

Vancomycin-resistant Enterococcus (VRE) are a serious health threat, causing 50,000 infections and 5,000 deaths each year in the United States (CDC, 2019). Multidrug-resistant VRE are becoming more prevalent, and treatment options for infections caused by these organisms are limited (Arias et al., 2010; CDC, 2019). Multidrug resistant VRE have been previously isolated from wastewater in Brazil, Canada, and Portugal (Araújo et al., 2010; de_Farias et al., 2022; Sanderson et al., 2019). Thirteen vancomycin-resistant Enterococcus faecium isolated from three stages of a Florida wastewater treatment plant were fully resistant to the following antibiotics: vancomycin, ampicillin, ciprofloxacin, erythromycin, tetracycline, and nitrofurantoin, and …

Jinetes De La Tormenta: The Invasive Influence Of Gender Constructs On The Journey Towards Selfhood And Societal Progress, Marissa Nicole Luquette

Theses and Dissertations

The novel Jinetes de la tormenta, written by Spanish author, Javier Castañeda de la Torre, presents gender as an obstacle to individuals in the ongoing process of self-discovery (as it relates to personal identity) and as the underlying source of human discontent in the technological age. Jinetes represents gender as a hindrance to the developing self through masculine and feminine binaries in European colonial languages and through the performative behavioral expectations tethered to the gendered body the theoretical works of Jacques Lacan and Hélène Cixous. The theoretical works of Jacques Lacan and Hélène Cixous establish the argument surrounding linguistic gender …

Women Gaze Back: Affect And Apparatic Disintegration In Funeral Parade Of Roses And Perfect Blue, Jessi Ivie

Theses and Dissertations

This thesis explores how films Funeral Parade of Roses (1969) by Toshio Matsumoto and Perfect Blue (1997) by Satoshi Kon subvert the male gaze of the camera by having the female main characters look back at the audience. The characters Mima (Perfect Blue) and Eddie (Funeral Parade) take control of the camera to subvert the hierarchical relationship between apparatus, audience, and characters. Through the lenses of apparatus and affect theory, these films show how the world of film includes the audience instead of precluding them. Experimental editing and heightened emotional situations unsettle the audience's voyeuristic position, bringing them closer to …

Cellular Energy Cycle Mediates An Advection-Like Forward Cell Flow To Support Collective Invasion, Jian Zhang, Jenna Mosier, Yusheng Wu, Logan Waddle, Paul V. Taufelele, Wenjun Wang, Heng Sun, Cynthia A. Reinhart-King

Biomedical Engineering Faculty Publications and Presentations

Collective cell migration is a model for nonequilibrium biological dynamics, which is important for morphogenesis, pattern formation, and cancer metastasis. The current understanding of cellular collective dynamics is based primarily on cells moving within a 2D epithelial monolayer. However, solid tumors often invade surrounding tissues in the form of a stream-like 3D structure, and how biophysical cues are integrated at the cellular level to give rise to this collective streaming remains unclear. Here, it is shown that cell cycle-mediated bioenergetics drive a forward advective flow of cells and energy to the front to support 3D collective invasion. The cell division …

Immunogenicity Of A Plasmodium Vivax Circumsporozoite Protein Vaccine, Eric Cruz Murphy

USF Tampa Graduate Theses and Dissertations

Malaria is a disease of global public health importance. Improving treatment methods focus on novel antimalarial drugs to combat parasite resistance and the development of vaccines with effective adjuvants. The current vaccine RTS,S/AS01 is moderately effective only against Plasmodium falciparum. There is no vaccine for Plasmodium vivax to date. The development and implementation of a vaccine against P. vivax would inhibit sporozoite infection of hepatocytes, block development into the blood stage and confer future immune protection. The circumsporozoite protein (CSP) is the most abundant on the surface of sporozoites and protein variants VK210 and VK247 are leading vaccine candidates for …

Protocol For Preclinical Evaluation Of Locoregionally Delivered Car T Cells In Patient-Derived Xenograft Models Of Brain Tumors, Benjamin Draper, Chantelle Bowers, Eleni Vassalou, Ailsa Greppi, John Anderson, Nabil Ahmed, Michael D Taylor, Laura K Donovan

Faculty, Staff and Students Publications

Here, we present a protocol for preclinical evaluation of locoregionally delivered CAR T cells in patient-derived xenograft models of primary, metastatic, and recurrent brain tumors. We provide instructions for isolating peripheral blood mononuclear cells (PBMCs), producing CAR T cells in conjunction with locoregional delivery, and preclinical trial design and analysis involving CAR T cells. Additionally, we describe comprehensive preclinical readouts and guidelines for critical endpoint sample collections. In line with clinical trial procedures, our protocol broadens available treatment modalities for direct clinical translation. For complete details on the use and execution of this protocol, please refer to Donovan et al …